• These nucleic acid probes play an important role in cloning new genes encoding low-abundance proteins. (creative-proteomics.com)
  • For example, genes encoding many important drug proteins used in the clinic. (creative-proteomics.com)
  • HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
  • Bioinformatic and comparative genomic analyses allowed us to identify genes encoding three candidate antibacterial effectors located within SPI-6 and two candidate effectors located within SPI-19. (frontiersin.org)
  • Of note, the genes encoding these effectors and immunity proteins are widely distributed in Salmonella genomes, suggesting a relevant role in interbacterial competition and virulence. (frontiersin.org)
  • We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
  • However, identification and functional analysis of such proteins is hampered by the lack of mutational systems in mycoplasmas and by a perceived limitation in translating recombinant mycoplasma genes containing UGA (Trp) codons in other eubacteria. (huji.ac.il)
  • To investigate further the potential role of mutated protein kinases in the development of TGCT and to characterize the prevalence and patterns of point mutations in these tumors, we have sequenced the coding exons and splice junctions of the annotated protein kinase family of 518 genes in a series of seven seminomas and six nonseminomas. (ox.ac.uk)
  • Comparative genomic hybridization and LOH analysis using SNP arrays demonstrated that the 13 TGCTs mutationally screened through the 518 protein kinase genes were uniformly aneuploid with consistent chromosomal gains on 12p, 8q, 7, and X and losses on 13q, 18q, 11q, and 4q. (ox.ac.uk)
  • These sequences are usually linked to M protein genes rather than the similar mrp or enn genes. (cdc.gov)
  • We report on the cloning and sequencing of two Tranosema rostrale ichnovirus (TrIV) genes, and assess their relatedness to TrV1, the gene encoding the most abundant TrIV transcript in last-instar Choristoneura fumiferana larvae parasitized by T. rostrale . (gc.ca)
  • in fact, the non-coding regions of all three genes show higher sequence identity than the coding regions, pointing to their common origin. (gc.ca)
  • The blocks of A. oryzae -specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. (nature.com)
  • The 37-Mb genome was predicted to contain a total of 12,074 genes encoding proteins with a length greater than 100 amino acid residues (see Methods). (nature.com)
  • These genes each encode proteins involved in pathways previously found essential for breast cancer development. (genomeweb.com)
  • Mendelian Randomization Analysis Identified Potential Genes Pleiotropically Associated with Polycystic Ovary Syndrome. (nih.gov)
  • Birgit Eisenhaber's research interest is focused on the discovery of molecular functions of previously uncharacterized proteincoding genes with special focus in proteins' posttranslational modifications. (a-star.edu.sg)
  • Next Generation Sequencing (NGS) is one technique that can measure one or more genetic variations as a laboratory diagnostic test, such as when used as a companion in vitro diagnostic test. (cms.gov)
  • CDC uses a de novo assembly approach for whole-genome sequence-based emm subtype determination. (cdc.gov)
  • Sequencing of the A. oryzae genome was accomplished using the whole-genome shotgun (WGS) approach. (nature.com)
  • Phylogenetic analysis of the three aspergilli using the whole-genome data showed that A. nidulans branched off earlier than A. oryzae and A. fumigatus 5 . (nature.com)
  • Analysis of worldwide sequence mutations in Zika virus proteins E, NS1, NS3 and NS5 from a structural point of view. (bvsalud.org)
  • We present here an analysis of all ZIKV sequences available in Genbank up to April 2016, studying the mutations in the whole polyprotein and their possible structural implications for the proteins E, NS1, NS3 and NS5. (bvsalud.org)
  • Here, using atomistic molecular dynamics simulation, we study the correlations between the RBD dynamics with physically distant residues in the spike protein, and provide a deeper understanding of their role in the infection, including the prediction of important mutations and of distant allosteric binding sites for therapeutics. (biorxiv.org)
  • Significant research effort for drug design against COVID-19 is focused on the receptor binding domain of the spike protein, although this region is prone to mutations causing resistance against therapeutics. (biorxiv.org)
  • 6 Significantly, the sites in S protein have been demonstrated to be vulnerable to acquire mutations. (biorxiv.org)
  • Sequencing of STK10 in an additional 40 TGCTs revealed no further mutations. (ox.ac.uk)
  • Mutations found in methyl CpG binding protein 2(MECP2) are associated with Rett syndrome. (cms.gov)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • The coding sequences (CDs) and protein sequences of newly reported dengue strains (DENV 1, DENV 2, DENV 3, and DENV 4) were obtained from the National center for Biotechnology Information (NCBI) nucleotide and protein databases. (als-journal.com)
  • The K a /K s ratio can be used to examine the action of natural selection on protein-coding regions, [5] [6] it provides information about the relative rates of nucleotide substitutions that change amino acids (non-synonymous substitutions) to those that do not change the encoded amino acid (synonymous substitutions). (wikipedia.org)
  • The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
  • Each protein has a characteristic number and sequence of amino acid residues. (creative-proteomics.com)
  • Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
  • Our model, based on time-independent component analysis (tICA) and protein graph connectivity network, was able to identify multiple residues, exhibiting long-distance coupling with the RBD opening dynamics. (biorxiv.org)
  • We applied time-independent component analysis (tICA) and protein connectivity network model, on all-atom molecular dynamics trajectories, to identify key non-RBD residues, playing crucial role in the conformational transition facilitating spike-receptor binding and infection of human cell. (biorxiv.org)
  • This database of trimmed 180 base entries corresponds to the first 50 residues of the mature M protein and the adjacent 10 C terminal residues of the signal sequence. (cdc.gov)
  • Sequence similarity networks of prokaryotic virus DJR MCP. (biomedcentral.com)
  • Protein sequences were clustered by the pairwise sequence similarity using CLANS. (biomedcentral.com)
  • The aim of this study is to bioinformatically correlate the coding sequences of four DENV strains to check their genetic & functional diversity on the basis of the similarity of the sequences. (als-journal.com)
  • The non-structural proteins NS1 and NS5 of all four DENV strains show more than 65 percent similarity index in 3D structure analysis. (als-journal.com)
  • it encodes a 102 amino acid protein showing 79% similarity to TrV1. (gc.ca)
  • Find regions of similarity between this sequence and other sequences using BLAST. (nih.gov)
  • Manual alignment and sequence analysis are facilitated by using group operations and amino acid color-coding reflecting amino acid similarity in physico-chemical and mutational properties, secondary structure propensities, etc. (bio.net)
  • Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)
  • Dynamics of p38 MAP kinase inferred from a structural ensemble using PCA is compared to intrinsic dynamics of the protein modeled using ANM. (bahargroup.org)
  • Workflow for comparative analysis of sequence evolution and structural dynamics is shown. (bahargroup.org)
  • Protein sequencing analysis can directly identify post-translational modifications that cannot be predicted from gene sequences, characterize recombinant proteins, and fragments of natural and recombinant proteins for structural and functional studies. (creative-proteomics.com)
  • In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
  • Our study provides essential structural information of unexplored proteins of the Hom family that can help in a better understanding of H. pylori pathogenesis. (nature.com)
  • Moreover, the structural analysis of ZIKV proteins , together with the mutational landscape of ZIKV and a structure-sequence comparison with other flaviviruses , allows the suggestion of regions that could be exploited as anti- ZIKV targets, including some allosteric sites found in the NS3 and NS5 proteins of DENV. (bvsalud.org)
  • RNA testing looks for changes in gene expression, and protein analysis assesses structural changes in the protein product of the gene. (jax.org)
  • The key focus in on integrating sequence, structural, and computational methods to elucidate key functional regions of viral and human RNA. (a-star.edu.sg)
  • Sequence analysis of the isolated gene predicted a hydrophilic protein, P101, containing three UGA codons and a putative signal peptide with an uncharacteristic cluster of positively charged amino acids near its C terminus. (huji.ac.il)
  • the alphabet is the 20 proteinogenic amino acids for proteins and the sense codons (i.e., the 61 codons that encode amino acids in the standard genetic code ) for aligned protein-coding gene sequences. (wikipedia.org)
  • In fact, substitution models can be developed for any biological characters that can be encoded using a specific alphabet (e.g., amino acid sequences combined with information about the conformation of those amino acids in three-dimensional protein structures [7] [8] ). (wikipedia.org)
  • Protein analyses examine the primary structure (amino acids) as well as the secondary folded structure, more complex tertiary structures, and protein interactions. (jax.org)
  • Comparative analysis of p38 MAP kinase dynamics from experiments (PCA) and theory (ANM). (bahargroup.org)
  • Sequence analysis of the protein kinase gene family in human testicular germ-cell tumors of adolescents and adults. (ox.ac.uk)
  • The protein kinase gene family is the most frequently mutated in human cancer. (ox.ac.uk)
  • Our results do not provide evidence for a mutated protein kinase implicated in the development of TGCT other than KIT. (ox.ac.uk)
  • Topology prediction shows that there are two N-glycosylation sites, three protein kinase C phosphorylation sites, two casein kinase II phosphorylation sites, four N-myristoylation sites, two amidation sites, and one ribosomal protein S11 signature in the RPS14 protein of the giant panda. (geneticsmr.com)
  • Results from comparative analysis of residue conservation, conformational mobility, and coevolutionary patterns for uracil-DNA glycosylase. (bahargroup.org)
  • Comparative analysis of dynamics of drug target proteins and model systems from experiments (PCA) and theory (ANM). (bahargroup.org)
  • Distributed Many-to-Many Protein Sequence Alignment Using Sparse Matrices. (crossref.org)
  • Substitution models are used to calculate the likelihood of phylogenetic trees using multiple sequence alignment data. (wikipedia.org)
  • Multiple sequence alignment (in this case DNA sequences) and illustrations of the use of substitution models to make evolutionary inferences. (wikipedia.org)
  • The majority of substitution models used for evolutionary research assume independence among sites (i.e., the probability of observing any specific site pattern is identical regardless of where the site pattern is in the sequence alignment). (wikipedia.org)
  • By capitalizing on this redundancy we show that, similarly to the alignment of protein sequences, unidentified MS/MS spectra can also be aligned for the identification of modified and unmodified variants of the same peptide. (nih.gov)
  • Subject: ANNOUNCEMENT: Protein Multiple Sequences Editor for Win 3.x/95 Dear All, With the increasing of protein sequence data, multiple alignment is one of the important techniques to understand protein structure-function organization and evolution. (bio.net)
  • ProMSED is an easy-to-use application for Windows 3.x/95 that performs automatic and manual multiple protein sequences alignment, alignment analysis and editing. (bio.net)
  • ProMSED loads up to eight protein families in time, adds sequences to existing alignment, combines sequences from different files, outputs the alignment in two formats, prints the alignment. (bio.net)
  • Tamura K, Dudley J, Nei M, Kumar S. Mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. (als-journal.com)
  • When it was fused with the N-terminally His-tagged protein, it gave rise to accumulation of an expected 22-kDa polypeptide, in good agreement with the predicted molecular weight. (geneticsmr.com)
  • In summary, these data strongly indicate that RA33 and A2 are the same protein, and thus identify on a molecular level a new autoantigen. (jci.org)
  • Introductory data analysis focusing on molecular biology data sets and examples and including basic programming skills using Python and basic statistics skills using R. Students with credit for MBB 243 may not take this course for further credit. (sfu.ca)
  • The purpose of this introductory data analysis course is to teach students in molecular biology or any students who will analyze molecular data, basic knowledge of molecular biology data types, data analysis methods including basic programming skills using Python, and basic statistics skills using R. (sfu.ca)
  • Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)
  • Our comprehensive molecular profiling approach precisely analyzes DNA, RNA, and protein biomarkers, revealing the highest quality molecular blueprint for evidence-based selection of the most appropriate cancer therapy. (carislifesciences.com)
  • Caris molecular profiling performs thorough molecular testing on DNA, RNA and proteins to identify the biomarkers driving a patient's tumor, and to compare the biomarkers with data from clinical studies around the world. (carislifesciences.com)
  • His group at the Bioinformatics Institute is primarily interested in the interplay of structure and function of RNA in gene regulation and infectious disease using statistical modeling, multi-omics data, and molecular simulations to reveal the structures and functional mechanisms of folded RNA and RNA-protein complexes. (a-star.edu.sg)
  • Increasing the number of sequences from three to seven significantly improved the predictive value of evolutionary computations. (nih.gov)
  • These data validate our hypothesis that detailed evolutionary analyses help predict the consequences of missense amino-acid variants. (nih.gov)
  • In biology, a substitution model , also called models of DNA sequence evolution , are Markov models that describe changes over evolutionary time. (wikipedia.org)
  • Estimates of evolutionary distances (numbers of substitutions that have occurred since a pair of sequences diverged from a common ancestor) are typically calculated using substitution models (evolutionary distances are used input for distance methods such as neighbor joining ). (wikipedia.org)
  • It is also necessary to assume a substitution model to estimate evolutionary distances for pairs of sequences (distances are the number of substitutions that have occurred since sequences had a common ancestor). (wikipedia.org)
  • The methods for sequence analysis of synthetic polymers differ from the sequence analysis of biopolymers (e. g. (wikipedia.org)
  • In fact, when applied to a set of spectra from cataractous lenses proteins from a 93-year old patient, spectral networks were able to capitalize on the highly correlated peaks in spectra from variants of the same peptide to rediscover the modifications identified by database search methods and additionally discovered several novel modification events. (nih.gov)
  • However, for a discrete number of patients whose results are uninformative, it may be possible to get a more definitive answer about the consequences of a variant using additional methods like RNA sequencing, protein analysis and predictive or functional studies. (jax.org)
  • The course covers the basics, principles and limitations for the most common methods for multivariate statistics (analysis of variance, principal component analysis and partial least squares regression), experimental design (super-saturated and factorial designs) and method validation with a focus on practical use. (lu.se)
  • The Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. (frontiersin.org)
  • Highly accurate long-read HiFi sequencing data for five complex genomes. (crossref.org)
  • Nanopore sequencing and the Shasta toolkit enable efficient de novo assembly of eleven human genomes. (crossref.org)
  • 5 , 7 - 9 More specifically, a study analyzing 10,022 SARS-CoV-2 genomes from 68 countries revealed 2969 different missense variants, with 427 variants in the S protein. (biorxiv.org)
  • Phylogenetic analysis of the L-proteins of members of the family Paramyxoviridae. (medscape.com)
  • Despite the small p16 sequence database, our calculations of high conservation correctly predicted loss of cell cycle arrest function in 75% of tested codons, and low conservation correctly predicted wild-type function in 80-90% of codons. (nih.gov)
  • The 180 bp subtype-encoding sequence consists of 60 codons encoding signal sequence (10 codons) plus the mature M protein (50 codons). (cdc.gov)
  • New emm types are assigned for more drastic changes within the first 30 codons encoding the mature M protein. (cdc.gov)
  • The majority of pathogenic variants are identified by DNA analysis. (jax.org)
  • But despite valuable contributions, the road to a understanding of protein behavior is still hurdled by significant difficulties in the extensive identification of unexpected post-translational modifications and highly modified peptides. (nih.gov)
  • Phased diploid genome assembly with single-molecule real-time sequencing. (crossref.org)
  • Hom (Helicobacter outer membrane) family of OMPs in H. pylori consists of four members (HomA, B, C and D). In the H. pylori genome jhp 0870 open-reading frame (ORF) that codes for HomB outer membrane protein is associated with many stomach diseases and is a novel co-marker for peptic ulcer disease (PUD) 9 , 10 . (nature.com)
  • Southern analysis suggests that each gene maps to a different TrIV genome segment, with homologous sequences apparently present on other segments. (gc.ca)
  • Southern and Northern analyses, using DNA and RNA extracted from infected Cf-124T cells, revealed the presence of both TrV1- and TrV4-carrying genome segments and transcripts, but neither DNA, at least in episomal form, nor mRNA persisted for more than a few days p.i. (gc.ca)
  • The genome of Aspergillus oryzae , a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced. (nature.com)
  • Syntenic analysis of the three aspergilli revealed the presence of syntenic blocks and A. oryzae -specific blocks of sequence (lacking synteny with the two other aspergilli) in a mosaic manner throughout the A. oryzae genome ( Fig. 1 ). (nature.com)
  • Thus, the increase in genome size seems to be due to an A. oryzae lineage-specific acquisition of sequence, rather than loss of sequence in A. nidulans and A. fumigatus . (nature.com)
  • This suggests that the genome size differences are largely due to sequence acquisition in A. oryzae . (nature.com)
  • Opens the Genome Data Viewer, NCBI's genome browser, at the genomic location at which this sequence is annotated. (nih.gov)
  • View this sequence in the context of other features annotated at the same location and browse to other genome regions. (nih.gov)
  • Sequence analysis of five tryptic peptides revealed that their sequences matched corresponding sequences of the A2 protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. (jci.org)
  • Other areas of interest are multi-omics and metagenomic analysis, particularly in the fields of animal health & aquaculture, and human immunology & ageing. (a-star.edu.sg)
  • The sequence patterns can become even more complex, when triads can be distinguished spectroscopically. (wikipedia.org)
  • Finds sub-sequences or patterns in the sequence and highlights the matching regions. (nih.gov)
  • sequences from their bird and mammal orthologs, the teleost versions specifically lack a characteristic QTQDQP phosphorylation site near the N-terminus. (usgs.gov)
  • [2] Substitution models are also necessary to simulate sequence data for a group of organisms related by a specific tree. (wikipedia.org)
  • This makes functional RNA structures challenging to approach with classical biomolecular structure elucidation techniques alone, and calls for new integrative data analysis approaches. (a-star.edu.sg)
  • investigated pathogenesis and immunological response caused by HomB protein in clinical patients and 190 H. pylori strain isolated from patients with peptic ulcer disease (PUD) or gastritis were evaluated for the clinical importance of homB . (nature.com)
  • Lee (2020) and Frésard (2019) demonstrated clinical utility in adding RNA sequencing to genomic testing for cohorts of individuals with undiagnosed Mendelian disease, increasing the diagnostic yield by 18% and 7.5%, respectively. (jax.org)
  • We compare the genetic and functional compatibility of selected gene sequences from four dengue strains by using various bioinformatics tools and software such as BLAST, MEGA 11.0, ProtParam, GOR4 and SWISS Model. (als-journal.com)
  • For this reason, using alternative primers for obtaining the emm sequence is discouraged (see emm typing protocol ). (cdc.gov)
  • Purification and partial sequencing of the nuclear autoantigen RA33 shows that it is indistinguishable from the A2 protein of the heterogeneous nuclear ribonucleoprotein complex. (jci.org)
  • Importantly, identification of P101 by direct analysis of its export function relied neither on prior identification of the mycoplasmal product nor on complete expression of the product from the cloned mycoplasma gene. (huji.ac.il)
  • See reference mRNA sequence for the NEIL2 gene (NM_145043.4). (nih.gov)
  • RNA-sequence analysis revealed that curcumin intake affected hepatic lipid metabolism via the peroxisome proliferator-activated receptor (PPAR) pathway, especially PPARα activation, resulting in increased Apoa2 mRNA expression. (elifesciences.org)
  • Synthetic polymers are a mixture of macromolecules of different length and sequence and are analysed via statistical measures (e. g. the degree of polymerization, comonomer composition or dyad and triad fractions). (wikipedia.org)
  • Multivariate Cox regression analysis demonstrated no independent prognostic significance of stromal Annexin A2 expression. (cancerindex.org)
  • This helps to avoid confounding of the 180-base M protein gene segment by the similar emm- like mrp and enn sequences (see CDC Streptococcus Laboratory GAS bioinformatic pipeline for S. pyogenes external icon ). (cdc.gov)
  • While some effector proteins specifically target bacterial or eukaryotic cells, others can target both types of cells (trans-kingdom effectors). (frontiersin.org)
  • Infection of human cells by the novel coronavirus (SARS-Cov-2) involves the attachment of the receptor binding domain (RBD) of the spike protein to the peripheral membrane ACE2 receptors. (biorxiv.org)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • Functional analyses use stem cells and other biological systems in vitro . (jax.org)
  • Most of the work on substitution models has focused on DNA/ RNA and protein sequence evolution. (wikipedia.org)
  • Amyloidosis is a group of diseases characterized by abnormal aggregation of proteins to form amyloid fibrils, and subsequent deposition in various tissues and organs, which can lead to severe functional failures. (elifesciences.org)
  • The M protein gene ( emm) encodes the cell surface M virulence protein responsible for at least 100 Streptococcus pyogenes M serotypes. (cdc.gov)
  • Through a whole-exome sequencing-based analysis of rare CNVs in 98 high-risk Northern Finnish breast cancer cases, the researchers aimed to tease out the role in breast cancer risk. (genomeweb.com)
  • In search for global principles that may explain the organization of the space of all possible proteins, we study all known protein sequences and structures. (aaai.org)
  • All 4670 clusters were then compared using either a structure metric (when 3D structures are known) or a novel sequence profile metric. (aaai.org)
  • The primary structure of a protein determines how the protein folds into a unique three-dimensional structure secondary, tertiary, and quaternary structures, which in turn determines the biological function of the protein. (creative-proteomics.com)
  • Conversely, broad spectrum therapeutics like drugs and monoclonal antibodies can be generated targeting these key distant regions of the spike protein. (biorxiv.org)
  • Large scale screening of therapeutic molecules and antibodies are underway aiming to target the spike protein and consequently prevent infection. (biorxiv.org)
  • Exhibiting high consistence in sequence and structure, S100 family members are interchangeable in function and they show a wide spectrum of biological processes, including proliferation, apoptosis, migration, inflammation and differentiation and the like. (cancerindex.org)
  • Using the PSI-BLAST procedure we defined 4670 clusters of related sequences in this space. (aaai.org)
  • Of these clusters, 1421 are centered on a sequence of known structure. (aaai.org)
  • In addition, there is limited information regarding the repertoire of effector proteins encoded within T6SS SPI-6 and T6SS SPI-19 gene clusters in S . Dublin. (frontiersin.org)
  • However, limitations of NMR spectroscopy are that it cannot, so far, provide information about the sequence distribution along the chain, like gradients, clusters or a long-range order. (wikipedia.org)
  • H. pylori attaches and colonizes to the human epithelium using some of their outer membrane proteins (OMPs). (nature.com)
  • Outer membrane proteins (OMPs) of H. pylori play a crucial role in the host-pathogen interaction, virulence and pathogenesis. (nature.com)
  • Outer membrane proteins (OMPs) are distinctive features of Gram-negative bacteria. (nature.com)
  • Proteins translocated across the single plasma membrane of mycoplasmas (class Mollicutes) represent important components likely to affect several interactions of these wall-less microbes with their respective hosts. (huji.ac.il)
  • Here we directly analyze a gene encoding a Mycoplasma hyorhinis protein capable of promoting its membrane translocation. (huji.ac.il)
  • TnphoA fusions expressing products that could translocate as much as 48 kDa of the P101 sequence (up to the first UGA codon) across the E. coli plasma membrane. (huji.ac.il)
  • Fusion proteins containing mature P101 sequences expressed mycoplasma epitopes and were found by cell fractionation and detergent phase partitioning to be integral membrane proteins in E. coli, suggesting a lack of signal peptide cleavage in this system. (huji.ac.il)
  • Deciding whether a missense allelic variant affects protein function is important in many contexts. (nih.gov)