Fission yeast Schizosaccharomyces pombeGeneGenomeATCCSubunitKinaseEncodesChromosomesMRNASignal transductionHeterochromatinFamily of proteinsYEASTOrganismTelomereSequenceRecombinant proteinsPathwaysRad51CytokinesisAminoReplicationCerevisiaeSerineUniProtDomains2018MitochondrialCellularTransformation2021MembraneEnzymesFunctionalGeneticDouble-StrandedPerturbationSpeciesInteractionsRegulatoryNuclearApproaches
Fission yeast Schizosaccharomyces pombe6
- In this study, we demonstrate that S-palmitoylation mediated by Erf2-Erf4 and Akr1 palmitoylacyltransferases is required at multiple meiotic stages in the fission yeast Schizosaccharomyces pombe . (life-science-alliance.org)
- Here, we report that Lem2 maintains genome stability in the fission yeast Schizosaccharomyces pombe. (nih.gov)
- Professor Forsburg studies how chromosome duplication and maintenance contributes to overall genome stability using a simple model genetic system, the fission yeast Schizosaccharomyces pombe . (usc.edu)
- The goal of this thesis was to characterize how cells regulate signaling of cytokinesis, both positively and negatively, during cell division using the fission yeast Schizosaccharomyces pombe as a model organism. (umassmed.edu)
- In the fission yeast Schizosaccharomyces pombe, the DNA damage response protein Brc1 binds phospho-histone H2A (γH2A)-marked chromatin during S-phase, but how Brc1 protects genome integrity remains unclear. (thebiogrid.org)
- The well-studied fission yeast Schizosaccharomyces pombe is the focus throughout, but the emerging model S. japonicus a larger, dimorphic species with several desirable characteristics is also covered. (cshlpress.com)
Gene9
- We isolated the Schizosaccharomyces pombe zfs1 gene as a multicopy suppressor of the sterility caused by overexpression of a double-stranded RNase. (elsevierpure.com)
- The deduced zfs1 gene product of 404 amino acids showed similarity to a mouse growth factor-inducible nuclear protein Nup475. (elsevierpure.com)
- S. pombe cells disrupted for the lem2(+) gene (lem2∆) showed slow growth and increased rate of the minichromosome loss. (nih.gov)
- The resulting duplication of the lnp1(+) gene, which encodes an endoplasmic reticulum membrane protein, suppressed lem2∆ phenotypes, whereas the lem2∆ lnp1∆ double mutant showed a severe growth defect. (nih.gov)
- Characterization of the bip gene of Aspergillus awamori encoding a protein with an HDEL retention signal homologous to the mammalian BiP involved in polypeptide secretion. (wikigenes.org)
- Here we describe the isolation and characterization of pfh1 + , a Schizosaccharomyces pombe gene that encodes a Pif1-like protein. (princeton.edu)
- Here we describe the isolation and characterization of pfh1+, a Schizosaccharomyces pombe gene that encodes a Pif1-like protein. (princeton.edu)
- Glutaminase liver isoform, mitochondrial is a protein in humans that is encoded by GLS2 gene. (cusabio.com)
- For complementary functional insights, we predicted Gene Ontology (GO) terms using machine learning methods exploiting protein-network and protein-homology data (NET-FF). (bvsalud.org)
Genome2
- These genetic interactions imply that Lem2 cooperates with the nuclear membrane protein network to regulate genome stability. (nih.gov)
- The genome sequence of Schizosaccharomyces pombe. (genscript.com)
ATCC6
- Mitochondrial distribution and morphology protein 12 OS=Schizosaccharomyces pombe (strain 972 / ATCC. (yeastrc.org)
- Mitochondrial distribution and morphology protein 12 OS=Candida glabrata (strain ATCC 2001 / CBS 138. (yeastrc.org)
- Mitochondrial distribution and morphology protein 12 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109. (yeastrc.org)
- Mitochondrial distribution and morphology protein 12 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS. (yeastrc.org)
- Mitochondrial distribution and morphology protein 12 OS=Candida albicans (strain SC5314 / ATCC MYA-2. (yeastrc.org)
- Lactose regulatory protein LAC9 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / N. (yeastrc.org)
Subunit1
- Disruption of zfs1 reduced both hypersensitivity of the ras1(Val17) mutant to the mating pheromone and uncontrolled mating response caused by mutational activation of Gpa1, the G protein a subunit coupled to the mating pheromone receptors. (elsevierpure.com)
Kinase6
- Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold. (mpg.de)
- 1) We first sought to characterize the positive timing mechanism that signals cytokinesis though a detailed investigation of Sid1p, a protein kinase essential for activation of ring constriction. (umassmed.edu)
- Protein kinases (PKs), MAP kinase kinase (MAPKK) subfamily, fungal PBS2-like proteins, catalytic (c) domain. (umbc.edu)
- The MAPKK subfamily is part of a larger superfamily that includes the catalytic domains of other protein serine/threonine kinases, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. (umbc.edu)
- The mitogen-activated protein (MAP) kinase signaling pathways are important mediators of cellular responses to extracellular signals. (umbc.edu)
- You can click on the 'Example' button to load the protein sequence of Schizosaccharomyces pombe Aurora-related kinase 1. (biocuckoo.org)
Encodes2
- A combination of mutations in Lem2 and Bqt4, which encodes a nuclear membrane protein that anchors telomeres to the nuclear membrane, caused synthetic lethality. (nih.gov)
- TSC2, encodes for the protein tuberin. (medscape.com)
Chromosomes3
- Inner nuclear membrane proteins interact with chromosomes in the nucleus and are important for chromosome activity. (nih.gov)
- the other was due to three different sites in the DNA of S. kambucha that interfere with the development of the spores that inherit S. pombe chromosomes. (elifesciences.org)
- Telomeres are specialized structures at the end of chromosomes and consist of stretches of repetitive DNA (5'-TTAGGG-3' in vertebrates and trypanosomatids) and associated proteins [ 5 ]. (biomedcentral.com)
MRNA1
- Schizosaccharomyces pombe Fe-sulfur cluster assembly protein Isu1 (isu1), mRNA. (genscript.com)
Signal transduction1
- Arrestins (abbreviated Arr) are a family of proteins which are important in the regulation of signal transduction G-protein coupled receptors, which at first was discovered, as part of a conserved two step mechanism for regulating the activity of G protein-coupled receptors (GPCR) in rhodopsin visual system by Ursula Kuhn found Scott court, and β-called Global Martin J. Lohse system and kidney and colleagues. (arrestins.com)
Heterochromatin2
- RNA interference (RNAi) is critical for the assembly of heterochromatin at Schizosaccharomyces pombe centromeres. (rcsb.org)
- We propose that Ser7 promotes cotranscriptional chromatin retention of hRNA by recruiting the RNA-chromatin connector protein Chp1, which facilitates RNAi-dependent heterochromatin formation. (unamur.be)
Family of proteins1
- This intrinsic GTPase activity of ras is stimulated by a family of proteins collectively known as 'GAP' or GTPase-activating proteins. (wikipedia.org)
YEAST3
- Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha , which mate to form viable hybrid diploids that efficiently complete meiosis, but generate few viable gametes. (elifesciences.org)
- have now looked at two species of fission yeast- S. pombe and S. kambucha -that share 99.5% of their DNA sequence. (elifesciences.org)
- The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. (cshlpress.com)
Organism1
- 3) Browse by species You can directly click on the species to browse proteins localized at different localizations for the organism. (biocuckoo.org)
Telomere2
- The importance of telomeric proteins for telomere maintenance has increased our interest in the identification of homologues within the genus Leishmania . (biomedcentral.com)
- The mammalian TRF1 and TRF2 proteins, for example, bind double-stranded telomeres via a Myb-like DNA-binding domain and are involved with telomere length regulation and chromosome end protection. (biomedcentral.com)
Sequence13
- The Ras GTPase-activating proteins are quite large (from 765 residues for sar1 to 3079 residues for IRA2) but share only a limited (about 250 residues) region of sequence similarity, referred to as the 'catalytic domain' or rasGAP domain. (wikipedia.org)
- Note: There are distinctly different GAPs for the rap and rho/rac subfamilies of ras-like proteins (reviewed in reference) that do not share sequence similarity with ras GAPs. (wikipedia.org)
- These sequences represent the protein coding region of the isu1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
- GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. (genscript.com)
- Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MDM12 . (yeastrc.org)
- The C-terminal domain of the 990-amino acid Schizosaccharomyces pombe Spt5 protein, composed of repeats of a nonapeptide motif (consensus sequence TPAWNSGSK), is necessary and sufficient for binding to the capping enzymes in vivo (in a two-hybrid assay) and in vitro. (mskcc.org)
- ClustalW and bl2seq sequence analysis showed that LaTRF shared sequence identity with the Trypanosoma brucei TRF (TbTRF) protein and had the same degree of sequence similarities with the dimerization (TRFH) and the canonical DNA-binding Myb-like domains of both mammalian TRFs. (biomedcentral.com)
- The swiss-prot protein sequence for schizosaccharomyces pombe clr4 is o60016. (shopambitionhustle.com)
- Blastp (protein blast): compares one or more protein query sequences to a subject protein sequence or a database of protein sequences. (shopambitionhustle.com)
- Pfam is a large collection of protein families, represented by multiple sequence alignments and hidden markov models (hmms). (shopambitionhustle.com)
- Blasting online sequence databases is a way to retrieve orthologs for a protein of interest. (shopambitionhustle.com)
- 3) BLAST search could be used to find the specific protein and/or related homologues by sequence alignment. (biocuckoo.org)
- Only one protein sequence in FASTA format is allowed per time. (biocuckoo.org)
Recombinant proteins1
- All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility. (arrestins.com)
Pathways4
- In the last several years, heterozygosity leading to haploinsufficiency for proteins involved in DNA repair pathways was shown to play a role in genomic instability and carcinogenesis after DNA damage is induced. (aacrjournals.org)
- Because the effect of haploinsufficiency for one protein is relatively small, we hypothesize that predisposition to cancer could be a result of the additive effect of heterozygosity for two or more genes, critical for pathways that control DNA damage signaling, repair or apoptosis. (aacrjournals.org)
- In the last few years, mounting evidence suggests that heterozygosity leading to haploinsufficiency for proteins involved in DNA repair pathways plays a role in genomic instability and carcinogenesis. (aacrjournals.org)
- NOTCH proteins regulate signaling pathways involved in cellular differentiation, proliferation and death. (refine.bio)
Rad511
- Other non-telomeric proteins, such as the DNA repair proteins Mre11 and Rad51, also play important roles at telomeres [ 7 , 8 ]. (biomedcentral.com)
Cytokinesis2
- 2) Secondly, we sought to define how cells negatively regulate cytokinesis through investigation of Dma1p, a spindle checkpoint protein implicated in inhibition of cytokinesis. (umassmed.edu)
- We conclude that signaling cytokinesis is tightly coordinated with mitosis in S. pombe by positive signals acting through Sid1p and the SIN, and under certain conditions, negative signals acting through Dma1p. (umassmed.edu)
Amino3
- In each case, the amino acid substitution only leads to small perturbations in the conformation of the protein. (ubc.ca)
- A number of actin-binding proteins, including spectrin, alpha-actinin and fimbrin, contain a 250 amino acid stretch called the actin binding domain (ABD). (embl.de)
- Proteins containing only a single amino terminal CH domain. (embl.de)
Replication2
- and the contribution of chromatin-modifying proteins to replication and repair. (usc.edu)
- Here we report that the non-homologous end-joining (NHEJ) protein Ku becomes critical for survival of replication stress in brc1∆ cells. (thebiogrid.org)
Cerevisiae2
- Unlike the two S. cerevisiae Pif1 subfamily proteins, the S. pombe Pfh1p was essential. (princeton.edu)
- Members of this group include the MAPKKs Polymyxin B resistance protein 2 (PBS2) from Saccharomyces cerevisiae, Wis1 from Schizosaccharomyces pombe, and related proteins. (umbc.edu)
Serine3
- Fatty acylation is a type of protein lipidation with the attachment of saturated and unsaturated fatty acids to the glycine, serine, lysine, or cysteine residues of proteins. (life-science-alliance.org)
- Palmitoylation targets several protein residues, including serine (O-palmitoylation) and cysteine (S-palmitoylation or N-palmitoylation when it occurs at the N-terminal of the protein). (life-science-alliance.org)
- PKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine or tyrosine residues on protein substrates. (umbc.edu)
UniProt1
- You can find most of the protein databases that were available here at the uniprot blast service. (shopambitionhustle.com)
Domains4
- By using NMR spectroscopy, we demonstrate that purified Cdc4p is a monomeric protein with two structurally independent domains, each exhibiting a fold reminiscent of the EF-hand class of calcium-binding proteins. (ubc.ca)
- Chp1 is a large protein with no recognizable domains, apart from its chromodomain. (rcsb.org)
- Actin binding domains present in duplicate at the N-termini of spectrin-like proteins (including dystrophin, alpha-actinin). (embl.de)
- There are 66728 CH domains in 45684 proteins in SMART's nrdb database. (embl.de)
20181
- There are at least six types of lipids including fatty acids, isoprenoids, sterols, phospholipids, glycosylphosphatidylinositol anchors, and lipid-derived electrophiles, which can be covalently attached to proteins ( Chen et al, 2018 ). (life-science-alliance.org)
Mitochondrial1
- Mitochondrial distribution and morphology protein 12 OS=Yarrowia lipolytica (strain CLIB 122 / E 150. (yeastrc.org)
Cellular5
- Posttranslational protein S-palmitoylation regulates the localization and function of its target proteins involved in diverse cellular processes including meiosis. (life-science-alliance.org)
- Together, these results highlight the versatility of the cellular processes in which protein S-palmitoylation participates. (life-science-alliance.org)
- Mutations of LEM-domain proteins are associated with laminopathy, but their cellular functions remain unclear. (nih.gov)
- To address this issue, primary mouse cells, haploinsufficient for one or two proteins, ATM and RAD9, related to the cellular response to DNA damage were examined. (aacrjournals.org)
- Experimental validation identified new proteins involved in cellular aging, showing that these predictions and phenomics data provide a rich resource to uncover new protein functions. (bvsalud.org)
Transformation2
- The results show that cells having low levels of both ATM and RAD9 proteins are more sensitive to transformation by radiation, have different DNA double-strand break repair dynamics and are less apoptotic when compared with wild-type controls or those cells haploinsufficient for only one of these proteins. (aacrjournals.org)
- Another conclusion from most of the cases is that animals or cells haploinsufficient for the specified proteins have higher transformation rates after DNA damage is induced, but when their DNA is not significantly damaged by exogenous sources, tumor development rates are the same as for their wild-type counterparts. (aacrjournals.org)
20211
- In mammalian cells, S-palmitoylate is added by a family of 23 transmembrane zinc finger DHHC (Asp-His-His-Cys)-containing protein acyl transferases and is removed by fatty acyl protein thioesterases ( Malgapo & Linder, 2021 ). (life-science-alliance.org)
Membrane2
- Lem2 and Man1 are conserved members of the LEM-domain nuclear membrane protein family. (nih.gov)
- Ras proteins are membrane-associated molecular switches that bind GTP and GDP and slowly hydrolyze GTP to GDP. (wikipedia.org)
Enzymes1
- Here we review the current knowledge of these enzymes, with an emphasis on the human proteins, and highlight recent discoveries that have implications far beyond the understanding of RNA metabolism itself. (ox.ac.uk)
Functional3
- This phenomenon can be explained if Cdcp4 has more than one essential function or, alternatively, if two mutant proteins assemble to form a functional complex. (ubc.ca)
- Its C-terminal region carried two putative zinc-fingers, both of which should be intact for the protein to be functional as the suppressor. (elsevierpure.com)
- These analyses exposed phenotypes for 3492 mutants, including 124 mutants of 'priority unstudied' proteins conserved in humans, providing varied functional clues. (bvsalud.org)
Genetic1
- However, genetic analysis demonstrated that maintenance of telomeric DNA was not the sole essential function of S. pombe Pfh1p. (princeton.edu)
Double-Stranded1
- proteins that bind specifically to single-stranded G-rich DNA, proteins that bind to double-stranded DNA and proteins that interact with telomeric factors. (biomedcentral.com)
Perturbation1
- Protein dynamics revealed by 19 F NMR protein perturbation experiments. (mpg.de)
Species1
- 2) Browse by multiple localizations With the default setting, you can directly click on the 'Submit' button to browse proteins localized at both centrosome and midbody among 144 species. (biocuckoo.org)
Interactions1
- Based on the structure of Cdc4p, possible models for interallelic complementation including interactions with partner proteins and the formation of a myosin complex with Cdc4p fulfilling the role of both an essential and regulatory light chain are proposed. (ubc.ca)
Regulatory1
- It is important to understand the functions and regulatory mechanisms of protein lipidation, which will advance our understanding of their pathological relevance, leading to strategies for targeting protein lipidation for therapeutic applications. (life-science-alliance.org)
Nuclear3
- A role of Akr1 S-palmitoylating the nuclear fusion protein Tht1 to function in karyogamy is identified. (life-science-alliance.org)
- Western blot and indirect immunofluorescence combined with fluorescence in situ hybridization showed that LaTRF, similarly to hTRF2, is a nuclear protein that also associates with parasite telomeres. (biomedcentral.com)
- The nuclear localization of LaTRF, its association and co-localization with parasite telomeres and its high identity with TbTRF protein, support the hypothesis that LaTRF is a Leishmania telomeric protein. (biomedcentral.com)
Approaches1
- We applied phenomics and machine-learning approaches with Schizosaccharomyces pombe for broad cues on protein functions. (bvsalud.org)