• Within the resolution of these immunodetection techniques, we show that proteins encoded by the SIR3, SIR4, and RAP1 genes colocalize in a statistically significant manner with Y' telomere-associated DNA sequences. (rupress.org)
  • In most eukaryotes studied, ubiquitin is encoded by two classes of genes: (i) The first comprises genes coding for a single copy of ubiquitin fused to ribosomal proteins (r-proteins), most commonly eL40 and eS31. (123dok.net)
  • The SEB1/SBH1 and the SSO genes encode components of the protein secretory machinery functioning at the opposite ends, ER translocation and exocytosis, respectively, of the secretory pathway in Saccharomyces cerevisiae. (vtt.fi)
  • Overexpression of these genes can rescue temperature‐sensitive (ts) growth defect of many sec mutants impaired in protein secretion. (vtt.fi)
  • Interestingly, KlSSO1 and KlSEB1 show similar enhancing effect on production of a secreted protein as the SSO and SEB1 genes of S. cerevisiae when overexpressed. (vtt.fi)
  • AMP-activated protein kinase (AMPK) is a master metabolic regulator for controlling cellular energy homeostasis. (rcsb.org)
  • The secreted recombinant fusion protein was detected in supernatant by SDS-PAGE and confirmed by western blot analysis using anti-HSA and anti-L7/L12 antibodies. (ac.ir)
  • Background S. cerevisiae Rad 51 protein (400 aa, 43 kDa) is a functional and structural homolog of E.coli RecA and human Rad51 proteins and plays a central role in DNA homologous recombination and recombination repair by promoting homologous DNA strand exchange reaction. (agrisera.com)
  • Samples: Recombinant Rad51 protein of Saccharomyces cerevisiae , analyzed by SDS-PAGE and visualized by Coomasie stain (1) , 10 ng of recombinant Rad51 protein of Saccharomyces cerevisiae (2), c rude extract of Saccharomyces cerevisiae strain BY4741 (3) were separated on a 12.5 % SDS-PAGE and blotted to a membrane using wet transfer. (agrisera.com)
  • Yet, Cdc34S130AS167A expression to a level comparable to the endogenous protein led to a uniform G1 arrest at restrictive temperature, like the arrest observed in the control strain cdc34-2ts. (unimib.it)
  • Cell fractionation analysis as well as studies using a yeast strain that is conditionally deficient in glycosylation demonstrate that TIP1 is a heavily modified membrane‐associated protein. (researchwithrutgers.com)
  • Here, we increased the secretion of a heterologous α-amylase, a model protein used for studying the protein secretory pathway in yeast, by moderately overexpressing SEC16, which is involved in protein translocation from the endoplasmic reticulum to the Golgi apparatus. (dtu.dk)
  • The clustering of telomeres and colocalization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae. (rupress.org)
  • This suggests that telomeres are clustered in vegetatively growing cells, and that proteins essential for telomeric silencing are concentrated at their sites of action, i.e., at telomeres and/or subtelomeric regions. (rupress.org)
  • Localization of telomeres and telomere-associated proteins in telomerase-negative Saccharomyces cerevisiae. (le.ac.uk)
  • Ubiquitin is a small eukaryotic protein of 76 amino acids whose name (i.e., it occurs ubiquitously) results from its remarkable evolutionary conservation [1]. (123dok.net)
  • Pulsed-SILAC experiments reveals that NatA-deficient strains consistently increase degradation of ribosomal proteins compared to wild type. (lu.se)
  • Local folding coupled to RNA binding in the yeast ribosomal protein L30. (expasy.org)
  • It is generated by proteolytic cleavage of precursor proteins in which it is fused either to itself, constituting a polyubiquitin precursor of head-to-tail monomers, or as a single N-terminal moiety to ribosomal proteins. (123dok.net)
  • Understanding the role of the ubiquitin fused to ribosomal proteins becomes relevant, as these proteins are practically invariably eS31 and eL40 in the different eukaryotes. (123dok.net)
  • We conclude that ubiquitin might serve as a cis-acting molecular chaperone that assists in the folding and synthesis of the fused eL40 and eS31 ribosomal proteins. (123dok.net)
  • Ubiquitin fusion to other r-proteins, such as P1 or P2, and even to non-ribosomal proteins such as actin, has been reported in diverse genera of rare single-celled algae as, for example, Bigelowiella [6]. (123dok.net)
  • The immunogenic B. abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. (ac.ir)
  • In order to construct yeast expressing vector for the tHSA-L7/L12 fusion protein, the L7/L12 ribosomal gene was amplified by PCR. (ac.ir)
  • The reference genome sequence of Saccharomyces cerevisiae: Then and now. (joplink.net)
  • Using pattern searches and position-dependent matrices, we have extracted the AT-hook motifs present in a non-redundant protein sequence database. (embl.de)
  • We have classified these motifs into three types according to their sequence similarity and have found that they are prevalent in many eukaryotic nuclear proteins in single or multiple copies. (embl.de)
  • The HMG family of proteins comprises members with multiple HMG domains that bind DNA with low sequence specificity, and members with single HMG domains that recognize specific nucleotide sequences. (embl.de)
  • Because many yeast proteins are similar in sequence and function to those found in other organisms, studies performed in yeast can help us to determine how a particular gene or protein functions in higher eukaryotes (including humans). (jove.com)
  • S. cerevisiae is an attractive model organism due to the fact that its genome has been sequenced, its genetics are easily manipulated, and it is very easy to maintain in the lab. (jove.com)
  • Now that we've learned a bit about S. cerevisiae as an organism, let's discuss what makes it a great model system for research. (jove.com)
  • Finally, the moderate overexpression of SEC16 was shown to improve the secretion of two other recombinant proteins, Trichoderma reesei endoglucanase I and Rhizopus oryzae glucan-1,4-α-glucosidase, indicating that this mechanism is of general relevance. (dtu.dk)
  • Ubiquitin functions as a reversible post-translational modifier of proteins to regulate many different cellular processes such as DNA repair, chromatin dynamics, cell cycle regulation, membrane and protein trafficking, endocytosis, autophagy, but most notably proteasome-dependent protein degradation [2-4]. (123dok.net)
  • Together these proteins shorten the end of the mRNAs, preparing the molecules for degradation. (elifesciences.org)
  • The proteins contain putative N‐terminal signal peptides as well as hydro‐phobic C‐terminal sequences, indicating that the proteins may be membrane bound. (researchwithrutgers.com)
  • A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
  • Here it is demonstrated that a TIP1 homologue, TIR1, which had been previously cloned as SRP1 (serine‐rich protein), is strongly induced by a downshift in growth temperature from 30 to 10°C. We further cloned TIR2, which is transcribed at a low basal level but is increased strongly by cold shock and, to a lesser extent, by heat shock. (researchwithrutgers.com)
  • RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
  • When telomere-associated proteins Sir2p, Sir3p and Rap1p were tagged, the same general trend was seen-Type I survivors retained the senescence/crisis state of protein localization, while Type II survivors were restored to wild type. (le.ac.uk)
  • In contrast to the popular SRP model, RNA seq analysis in combination with qPCR and microscopic data revealed that mRNAs coding for nuclear-related proteins can also localize to ER. (uni-tuebingen.de)
  • By deleting ER-associated RBPs such as Mrn1p, Whi3p, Pbp2p, Slf1p, and Ngr1p, I see an upregulation of mRNAs coding for nuclear-encoded MRPs and at the same time a loss in the protein levels of Mrpl39p, Mrpl44p, and Img2p. (uni-tuebingen.de)
  • A set of nuclear proteins in SACCHAROMYCES CEREVISIAE that are required for the transcriptional repression of the silent mating type loci. (jefferson.edu)
  • Inner nuclear membrane protein LBR preferentially interacts with DNA secondary structures and nucleosomal linker. (medlineplus.gov)
  • However, the pleiotropic nature of these effects has hampered our understanding of the functional impact of protein Nt-acetylation. (lu.se)
  • Furthermore, AT-hook motifs are frequently associated with known functional domains seen in chromatin proteins and in DNA-binding proteins (e.g. histone folds, homeodomains and zinc fingers). (embl.de)
  • Biallelic hypomorphic variants in PPA2 , encoding the mitochondrial inorganic pyrophosphatase 2 protein, have been recently identified in individuals presenting with sudden cardiac death, occasionally triggered by alcohol intake or a viral infection. (nature.com)
  • Saccharomyces cerevisiae (commonly known as baker's yeast) is a single-celled eukaryote that is frequently used in scientific research. (jove.com)
  • Saccharomyces cerevisiae , otherwise known as baker's yeast, is one of the many model organisms studied in laboratories all over the world. (jove.com)
  • In accordance with the high homology level of the secretory pathway proteins in different yeast species, the polyclonal antibodies raised against S. cerevisiae Seb1p, Sso2p and Sec4p can detect homologous proteins in cell lysates of K. lactis and Pichia pastoris, the latter also in Candida utilis. (vtt.fi)
  • Our data are in agreement with a role for Nt-acetylation in promoting stability for parts of the proteome by enhancing the avidity of protein-protein interactions and folding. (lu.se)
  • The interactions of proteins with chromosomal DNA control a variety of cellular processes, including gene transcription, DNA packing, replication, recombination, and DNA repair. (psu.edu)
  • High mobility group (HMG) box domains are involved in binding DNA, and may be involved in protein-protein interactions as well. (embl.de)
  • HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. (embl.de)
  • The testis expressed LC3-II protein, which was located within spermatids at different stages of differentiation and indicated active autophagy. (frontiersin.org)
  • Autophagy is the main cellular process responsible for degrading defective organelles and long-lived proteins. (frontiersin.org)
  • Vac8 controls vacuolar membrane dynamics during different autophagy pathways in Saccharomyces cerevisiae. (ruhr-uni-bochum.de)
  • In Saccharomyces cerevisiae a specialization of the two catalytic subunits (α e α') was shown: α subunit is involved in cell polarity, while α' subunit is linked to cell-cycle regulation and was shown to be fundamental both in G1 phase and in mitosis. (unimib.it)
  • Ubiquitin is a highly conserved small eukaryotic protein. (123dok.net)
  • Most of the seminal studies on the regulatory mechanisms of autophagosome biogenesis were conducted on Saccharomyces cerevisiae or culture cells in vitro ( Hamasaki and Yoshimori, 2010 ). (frontiersin.org)
  • Protein N-terminal (Nt) acetylation is one of the most abundant modifications in eukaryotes, covering ~50-80 % of the proteome, depending on species. (lu.se)
  • Background: Iwr1, a protein conserved throughout eukaryotes, was originally identified by its physical interaction with RNA polymerase (Pol) II. (johnshopkins.edu)
  • The G protein-coupled receptor (GPCR) family is composed of hundreds of members and is expressed in eukaryotes. (tennessee.edu)
  • Interestingly, many proteins found in yeast share similar sequences with proteins from their fellow Eukaryotes. (jove.com)
  • By investigating the function of a given protein in yeast, researchers gain insight into the protein's function in higher eukaryotes, such as us, humans. (jove.com)
  • In particular, among these, S130 and S167, within the catalytic domain of the protein, are highly conserved among Cdc34 homologues in various organisms, and were identified as phosphorylated sites in vivo in a CK2-dependent manner. (unimib.it)
  • The encoded Kluyveromyces proteins are up to 70% identical with the S. cerevisiae homologues at the amino acid level and can functionally replace them. (vtt.fi)
  • Here we employ a multi-dimensional proteomics approach to analyze Saccharomyces cerevisiae lacking NatA activity, which causes global proteome remodeling. (lu.se)
  • The deubiquitination of the PTS1-import receptor Pex5p is required for peroxisomal matrix protein import. (ruhr-uni-bochum.de)
  • ATP-driven processes of peroxisomal matrix protein import. (ruhr-uni-bochum.de)
  • Regulation of peroxisomal matrix protein import by ubiquitination. (ruhr-uni-bochum.de)
  • The AT-hook is a small DNA-binding protein motif which was first described in the high mobility group non-histone chromosomal protein HMG-I(Y). Since its discovery, this motif has been observed in other DNA-binding proteins from a wide range of organisms. (embl.de)
  • It is most interesting that this motif seems to be quite specific to known or predicted chromosomal/DNA-binding proteins, suggesting that it may act as a versatile minor groove tether. (embl.de)
  • We have analyzed the phenotypic effects of a genomic ubi1∆ub-HA ubi2∆ mutant, which expresses a ubiquitin-free HA-tagged eL40A protein as the sole source of cellular eL40. (123dok.net)
  • Saccharomyces cerevisiae expresses three phospholipid hydroperoxide glutathione peroxidases. (microbiologyresearch.org)
  • Principal Findings: Here, we identify Iwr1 in a genetic screen designed to uncover proteins involved in Pol III transcription in S. cerevisiae. (johnshopkins.edu)
  • Specific cloned DNA sequences can be assembled with proteins isolated from cellular extracts to provide details on the binding of each factor and its effect on the overall structure and activity of the complex. (psu.edu)
  • The predicted protein sequences are also consistent with extensive O‐mannosylation as well as glycosyl‐phosphatidyl inositol (GPI) membrane anchoring. (researchwithrutgers.com)
  • Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)
  • These proteins are often homologous, and their similar sequences indicate that the organisms share a common ancestor. (jove.com)
  • 2006), and Cdc34, the E2 enzyme required for the ubiquitination of many cell-cycle proteins (among which Sic1), which is phosphorylated by CK2 on Ser207, Ser216 e Ser282 (Pyerin et al. (unimib.it)
  • Our initial assays with the L. starkeyi secretome expressing differing Tr EGII domains fused to a chimeric Talaromyces emersonii - T. reesei CBHI ( TeTr CBHI) showed that the complete Tr EGII enzyme, including the glycoside hydrolase (GH) 5 domain is required for increased expression level of the fusion protein when linked to CBHI. (biomedcentral.com)
  • Expression of the L1 protein using recombinant DNA technology produces noninfectious virus-like particles (VLPs). (cdc.gov)
  • Here, a Saccharomyces cerevisiae sod1 Δ mutant (defective for the Cu,Zn-superoxide dismutase) was found to be hypersensitive to Cr(VI) toxicity under aerobic conditions, but this phenotype was suppressed under anaerobic conditions. (microbiologyresearch.org)
  • In contrast, S. cerevisiae msra Δ ( mxr1 Δ) and msrb Δ ( ycl033c Δ) mutants defective for peptide methionine sulfoxide reductase (MSR) activity exhibited a Cr sensitivity phenotype, and cells overexpressing these enzymes were Cr-resistant. (microbiologyresearch.org)