• The diagnosis of many infectious diseases, both viral and bacterial, may include the use of reverse transcriptase-polymerase chain reaction (RT-PCR). (medscape.com)
  • Next-generation sequencing analyses revealed the expression of TRPV1 in several native breast cancer tissues, which was subsequently validated via reverse transcriptase-polymerase chain reaction. (dovepress.com)
  • At the end of 8 weeks, all rats were sacrificed to obtain the kidney tissues for histological examination and reverse transcription polymerase chain reaction (RT-PCR) was used to analyze the transcriptional levels of nephrin and podocin genes. (biomedcentral.com)
  • Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
  • EXPERIMENTAL DESIGN: To identify the genes involved in pancreatic cancer metastasis, we analyzed the gene expression profiles between highly metastatic Colo357L3.6pl and parental Colo357FG pancreatic cancer cell lines using cDNA microarrays and confirmed differential gene expression by reverse transcription-PCR, Western blotting, and immunologic analysis of 54 samples from pancreatic cancer patients. (duke.edu)
  • Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
  • The initial PCR reaction amplifies a region of the HCV genome (External primers). (cdc.gov)
  • In some PCR reactions multiple sequences have identical nucleotide sequences, for example if five PCR products have identical sequences a 5 will be found in this column. (cdc.gov)
  • The discovery of this reaction earned Kary Mullis, a US scientist, the Nobel Prize for chemistry in 1993. (island.lk)
  • A: Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of FGK gene expression. (xenbase.org)
  • Pooled analysis of 16 studies (3818 patients) estimated a sensitivity of 87.8% (95% CI 81.5% to 92.2%) for an initial reverse-transcriptase PCR test. (bmj.com)
  • Is the Subject Area "Reverse transcriptase-polymerase chain reaction" applicable to this article? (plos.org)
  • We report the correlation of results of reverse transcriptase polymerase chain reaction (RT-PCR) and immunofluorescent serologic testing for SARS-CoV in 1,048 cases investigated for SARS in the first 5 weeks after the first-generation diagnostic tests became available in Hong Kong. (cdc.gov)
  • The genome of the virus is a long chain of four 'letters' of the genetic alphabet called RNA (most genomes, such as ours, are DNA). (island.lk)
  • Vomitus and stool samples from affected passengers were tested for norovirus by reverse transcriptase-polymerase chain reaction. (cdc.gov)
  • Hepatitis C RNA: Testing for HCV RNA by reverse-transcriptase ¬polymerase-chain reaction (RT-PCR) amplification of the 5' noncoding region was performed on anti-HCV positive samples. (cdc.gov)
  • Samples found to be negative for HCV RNA were extracted a second time by the same procedure with an additional incubation at 50 degrees Celsius for 45 minutes with 25 units of reverse transcriptase (Boehringer Mannnheim, Inndianapolis) and 10 units of RNAsin (Boehringer Mannnheim). (cdc.gov)
  • HLA-G expression was analysed in thyroid tissue taken from six patients with GD and one with HT by reverse transcriptase polymerase chain reaction. (bmj.com)
  • These results were validated by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting, confirming a concentration-dependent increase in HMOX1 and a decrease in THBS1 expression in ASC following iAs exposure. (nih.gov)
  • 26. Monitoring of minimal residual disease (MRD) by real-time quantitative reverse transcription PCR (RQ-RT-PCR) in childhood acute myeloid leukemia with AML1/ETO rearrangement. (nih.gov)
  • NP swabs were collected, RNA extracted, and SARS-CoV-2 testing performed using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). (medrxiv.org)
  • Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
  • PCR or the Polymerase Chain Reaction is a technique, which is widely used in various scientific fields including molecular biology, diagnostics, genetics, forensic science, and paternity testing among others. (brighthub.com)
  • On July 10, the hospital laboratory sent a blood culture isolate to Arizona State Public Health Laboratory (ASPHL) and Yersinia pestis was presumptively identified on July 12 using reverse transcriptase polymerase chain reaction testing. (cdc.gov)
  • A single center study reported that 7.41% of COVID-19 patients re-tested positive for SARS-CoV-2 RNA by real-time reverse transcriptase polymerase chain reaction (RT-PCR) test after discharge 8 , and this finding has challenged the current hospital discharge criteria for containing the pandemic. (nature.com)
  • Objective: To ascertain whether reverse transcriptase polymerase chain reaction (RT-PCR) cycle amplifications until detection, the cycle threshold (Ct), could help inform return to work (RTW) strategies for health care worker s (HCWs) recovering from COVID-19 infection. (cdc.gov)
  • 25. Rapid quantitative detection of BCR-ABL transcripts in chronic myeloid leukemia patients by real-time reverse transcriptase polymerase-chain reaction using fluorescently labeled probes. (nih.gov)
  • The detection of epithelial cells in dried bloodstains by reverse transcriptase-polymerase chain reaction is based on cell- and tissuespecific gene expression. (astm.org)
  • [ 1 ] The resultant complementary DNA is amplified by polymerase chain reaction (PCR). (medscape.com)
  • Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)
  • In this technique, RNA molecule is transcribed into complementary DNA, or cDNA using an enzyme reverse transcriptase and the cDNA is further modified by the PCR technique. (brighthub.com)
  • In PCR, a thermostable DNA polymerase is used to amplify target DNA 2-fold with each temperature cycle. (medscape.com)
  • At this temperature, the DNA polymerase enzyme works well and extends the chain further. (brighthub.com)
  • Generation of the primary antibody repertoire requires V(D)J recombination of hundreds of gene segments in the immunoglobulin heavy chain (Igh) locus. (babraham.ac.uk)
  • Tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) based on reverse transcriptase polymerase chain reaction (RT-PCR) are being used to rule out infection among high-risk persons, such as exposed inpatients and health care workers. (nih.gov)
  • Anaphylaxis and anaphylactic shock (including fatal cases), and other severe acute hypersensitivity reactions have been reported. (nih.gov)
  • The machine heats and cools the test tubes present in the reaction mixture. (brighthub.com)
  • Our findings suggest that such peptides may be used in reversing or halting the neurodegenerative process observed in neurodegenerative diseases, such as the peripheral neuropathy associated with type 2 diabetes mellitus and Alzheimer's and Parkinson's diseases. (aspetjournals.org)
  • Phosphorylation regulates, in a process called state transition, a profound reorganization of the electron transfer chain and remodeling of the thylakoid membranes. (unige.ch)
  • The study evaluated a combined antigen-antibody based test for rapid, sensitive and specific diagnostic methodology comparable to polymerase chain reaction. (uwi.edu)
  • Previous significant hypersensitivity reaction to Synagis. (nih.gov)