• In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. (wikipedia.org)
  • This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. (nih.gov)
  • Several of the SNF and SWI genes of Saccharomyces cerevisiae encode proteins that are involved in the regulation of transcriptional activation. (bdbiosciences.com)
  • The retinoblastoma protein, Rb, stimulates the transcription of a number of genes. (bdbiosciences.com)
  • In particular, we will discuss the modifications in intrinsic and extrinsic apoptotic pathways caused by proteins encoded by HPV early genes. (mdpi.com)
  • Consequently, while Smad protein are intrinsically transcriptional activators, the transcriptional end result of buy BV-6 their focus on genes often depends upon the transcriptional companions connected with Smads [24]. (molecularcircuit.com)
  • Significantly, binding of 53BP1 to methyl K810 occurs on E2 promoter binding factor target genes and allows pRb activity to be effectively integrated with the DNA damage response. (ox.ac.uk)
  • During expression of protein-coding genes, pre-mRNAs are transcribed in the nucleus and undergo several RNA-processing steps. (biomedcentral.com)
  • Pocket proteins Rb, p107 and p130 play central roles in cell cycle progression and differentiation. (eu.org)
  • The LxDLFD motif ( LIG_RB_pABgroove_1 ) mediates binding to a highly conserved deep groove formed in the interface between the A and B subdomains of the Rb, p107 and p130 pocket domains. (eu.org)
  • A phosphorylation downstream to the core motif acts as a switch that binds to a positively charged pocket only present in p107/p130. (eu.org)
  • This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)
  • This gene encodes the GA-binding protein transcription factor, beta subunit. (nih.gov)
  • The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4 , is also a component of several other protein complexes involved in chromatin remodeling. (nih.gov)
  • Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
  • It is found as a subunit of protein complexes that are in involved in the enzymatic modification of histones including the Mi2 and Sin3 histone deacetylase complexes and the polycomb repressive complex 2. (lookformedical.com)
  • Oxr1p belongs to the group of TLDc domain containing proteins that are highly conserved in higher eukaryotes. (upstate.edu)
  • The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes. (lookformedical.com)
  • These data suggest that various nuclear mRNA export pathways, which may be indicated by different adaptor RNA binding proteins, exist in higher eukaryotes. (biomedcentral.com)
  • The LxCxE motif binds to all pocket proteins and is present in chromatin regulators such as HDAC and KDM5A and in viral proteins. (eu.org)
  • Pocket proteins play essential roles in cell cycle progression, quiescence and differentiation, and their functional disruption is associated with human cancer. (eu.org)
  • Thus, DP-4 forms a heterodimer with E2F subunits, binds to the E2F site and associates with pocket proteins including pRb. (ox.ac.uk)
  • Retinoblastoma-like 1 (p107), also known as RBL1, is a protein that in humans is encoded by the RBL1 gene. (wikipedia.org)
  • LIG_RB_pABgroove_1 (LxDLFD), LIG_RB_LxCxE_1 (LxCxE) and LIG_RBL1_LxSxE_2 (LxSxE).The LxDLFD motif binds to a deep groove formed between the A and B subdomains and is present in E2F family transcription factors (E2F1-5). (eu.org)
  • Addgene: Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties. (addgene.org)
  • Endoplasmic reticulum (ER) is an essential organelle responsible for protein synthesis, folding, post-translational modification of proteins and protein trafficking in eukaryotes ( 8 ). (spandidos-publications.com)
  • Among oncogenic viruses are those in the Polyomaviridae family, whose members infect an array of vertebrate species, including birds, humans, nonhuman primates, bovids, rodents, and sea lions ( 1 , 2 ). (cdc.gov)
  • They further link to the activation of protein kinase C- (PKC-) induced generation of reactive oxygen species (ROS) [ 6 , 7 ], which further mediates the activation of downstream transcription factor nuclear factor kappa-light-chain enhancer of activated B cells (NF- κ B). Thus, the main treatments of DN refer to modulate glycemic and blood pressure through insulin and RAS inhibitors. (hindawi.com)
  • Although the senescent cells remain viable, they show typical changes with enlarged and flattened cell bodies, apoptosis resistance, increased activity of senescence-associated β -galactosidase (SA- β -gal), and upregulation of cyclin-dependent kinase (CDK) inhibitors including p16 INK4A , ARF proteins, and p21 [ 13 - 16 ]. (hindawi.com)
  • Cyclin-dependent kinases (CDKs), are members of the serine-threonine protein kinase family and are responsible for taking control of cell cycle regulation in eukaryotic cells. (spandidos-publications.com)
  • inositol-requiring enzyme 1 (IRE1α), PRKR-like ER kinase (PERK) and activating transcription factor-6 (ATF-6). (spandidos-publications.com)
  • Together, we demonstrate that Ocln is a downstream target of Src kinase and promotes LD secretion by binding to BTN1a1 and XOR. (bvsalud.org)
  • The NEMO protein is required for the activation of the kinase complex. (medscape.com)
  • The various markers that enable assessment of the progression of preneoplastic lesions to spindle cell carcinoma include the p16 protein, which halts the cell cycle and induces apoptosis by pRb-mediated phosphorylation of cyclin-dependent kinase 4 (CDK4). (bvsalud.org)
  • The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. (wikipedia.org)
  • Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. (wikipedia.org)
  • Lysine methylation-dependent binding of 53BP1 to the pRb tumor suppressor. (ox.ac.uk)
  • The retinoblastoma tumor suppressor protein pRb is a key regulator of cell cycle progression and mediator of the DNA damage response. (ox.ac.uk)
  • [ 4 ] The E6 protein product of these high-risk HPVs binds to the tumor suppressor protein p53, which is thought to disrupt the p53-dependent control of the cell cycle. (medscape.com)
  • [ 8 ] Research into the genome of MCV is expanding and specific proteins are being explored for their clinical utility. (medscape.com)
  • Furthermore, because MGMT protein loss may be a result of promoter hypermethylation, it was reported that methylation of the MGMT promoter in gliomas is a useful predictor of the responsiveness to alkylating agents ( 7 ). (aacrjournals.org)
  • Activation of the transcription factor nuclear factor KB (NF-KB) requires the NEMO protein. (medscape.com)
  • Differential pull-down experiments with H3-tails synthesized from l- and d-amino acids uncovered that histone acetyltransferase 1 (HAT1) and retinoblastoma-binding protein 7 (RBBP7) are part of the protein network, which interacts with the unmodified H3-tail. (edu.sa)
  • Users from the TGF-superfamily are TGF-is secreted like a latent precursor: After secretion the pro-domain (latency connected proteins, LAP) binds and inactivate the ligand, permitting its association with inhibitory latent TGF binding protein (LTBPs) that focus on the complicated towards the ECM where in fact the latent TGF-is sequestered. (molecularcircuit.com)
  • The ligand binds a sort II receptor, which phosphorylates somebody type I receptor, which propagates the sign in the cell via phosphorylation of downstream Smad-dependent and -self-employed procedures [20]. (molecularcircuit.com)
  • Proteins that enhance gene expression when associated with ligand bound activated NUCLEAR RECEPTORS. (bvsalud.org)
  • Inactivation of Rb may contribute to many human malignancies including familial retinoblastoma, small-cell lung carcinomas, cervical carcinomas, prostate carcinomas, breast carcinomas, and some forms of leukaemias ( Burkhart,2008 ). (eu.org)
  • Nutlin-3a is an MDM2 inhibitor that is under investigation in preclinical models for a variety of pediatric malignancies, including retinoblastoma, rhabdomyosarcoma, neuroblastoma, and leukemia. (nih.gov)
  • The RBBP1 Tudor domain structure has a significant area of positively charged surface, which reveals a capability of the RBBP1 Tudor domain to bind nucleic acids. (rcsb.org)
  • Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. (lookformedical.com)
  • The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
  • Transfected Brm, expressed in cells lacking endogenous protein, can cooperate with the glucocorticoid receptor (GR) in transcriptional activation. (bdbiosciences.com)
  • Singh P, Coe J, Hong W. A role for retinoblastoma protein in potentiating transcriptional activation by the glucocorticoid receptor. (bdbiosciences.com)
  • The latest report E3 Ubiquitin Protein Ligase Mdm2 - Pipeline Review, H1 2020, outlays comprehensive information on the E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)
  • The DNA binding mode and key interaction residues were analyzed in detail based on a model structure of the Tudor domain-dsDNA complex, built by HADDOCK docking using the NMR data. (rcsb.org)
  • The DNA-binding residues are conserved in Tudor domains of the RBBP1 protein family, resulting in conservation of the DNA-binding function in the RBBP1 Tudor domains. (rcsb.org)
  • These 18 residues CCHC zinc finger domains are mainly found in the nucleocapsid protein of retroviruses. (embl.de)
  • Multiple posttranslational modifications (PTMs) of histone proteins including site-specific phosphorylation of serine and threonine residues govern the accessibility of chromatin. (edu.sa)
  • Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. (embl.de)
  • The LxCxE and LxSxE motifs bind to a highly conserved cleft in the B subdomain. (eu.org)
  • Source of all serum proteins is from USDA inspected abattoirs located in the United States. (bdbiosciences.com)
  • Using absorption spectroscopy and NMR we demonstrate that the CCHC motifs each bind one equivalent of zinc with high affinity. (embl.de)
  • We show that full-length and C-terminal Nanos proteins bind to RNA in vitro with high affinity, but with little sequence specificity. (embl.de)
  • Thus, we include a final wild-card position, although the role of this position on binding affinity has not been tested. (eu.org)
  • Extensive interactions in the N-terminal flanking region of E2F increase Rb-binding affinity ( Lee,2002 ). (eu.org)
  • Tissue culture supernatant is purified by either protein A/G or affinity purification methods. (bdbiosciences.com)
  • A retinoblastoma-binding protein that has an affinity for core HISTONES. (lookformedical.com)
  • The effects of POP inhibition and knockdown on the proliferation of cultured human estrogen receptor-positive (ER+) MCF7 and T47D, and ER-negative (ER-) MDA-MB-231 breast cancer cell lines and the MCF12A non-tumorigenic epithelial cell line were tested by analyzing their influence on cell proliferation (WST-1 assay), cell viability (trypan blue exclusion assay), and cell cycle arrest (cell cycle analysis, cell cycle regulator proteins expression). (cancerindex.org)
  • It promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. (researchandmarkets.com)
  • 11. Amsel, A. & Roussel, J. Motivational properties of ences in post-transcriptional processes spark interest in the development of frustration: I. Effect on a running response of the (protein redistribution, degradation), pharmacotherapies that selectively reg- addition of frustration to the motivational com- plex. (lu.se)
  • Electrostatic interactions mediate the binding of the Tudor domain with DNA, which is consistent with NMR experiments performed at high salt concentration. (rcsb.org)
  • As the assembly was carried out in the absence of ATP, this suggests that formation of the holoenzyme from the individual subcomplexes is solely driven by protein-protein interactions at the interface. (upstate.edu)
  • Unlike reversible disassembly, which requires ATP hydrolysis on V 1 to break protein-protein interactions, Oxr1p mediated disassembly of V-ATPase is ATP independent and therefore a novel mode of activity regulation. (upstate.edu)
  • Alternatively nuclear receptor coactivators can function as adaptor proteins that bring nuclear receptors into close proximity with transcriptional complexes. (bvsalud.org)
  • Analysis of nanos mutants reveals that a small, evolutionarily conserved, C-terminal region is essential for Nanos function in vivo, while no other single portion of the Nanos protein is absolutely required. (embl.de)
  • nanos mutations disrupting metal binding at either of these two sites in vitro abolish Nanos translational repression activity in vivo. (embl.de)
  • Mutations affecting the hunchback mRNA target sites for Nanos-dependent translational repression were found to disrupt translational repression in vivo, but had little effect on Nanos RNA binding in vitro. (embl.de)
  • Moreover, blocking LD formation by targeting DGAT-1 in vivo inhibited virus replication and inflammation in the brain. (bvsalud.org)
  • Brm and Rb interact in vitro and in vivo , requiring the Rb-pocket domain and the consensus Rb-binding motif of Brm. (bdbiosciences.com)
  • To study the role of THOC5/Fms interacting protein in vivo , we generated THOC5/Fms interacting protein knockout mice. (biomedcentral.com)
  • This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. (novusbio.com)
  • Biological assembly 1 assigned by authors. (rcsb.org)
  • E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)
  • E3 ubiquitin-protein ligase Mdm2 is a protein encoded by the MDM2 gene. (researchandmarkets.com)
  • The report provides a snapshot of the global therapeutic landscape for E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)
  • The report reviews latest news and deals related to E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)
  • Identify and understand the targeted therapy areas and indications for E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)
  • We now show that HGE bacteria, and the HGE-44 protein, induce IL-8 secretion in a promyelocytic (HL-60) cell line that has been differentiated along the neutrophil lineage with retinoic acid and in neutrophils. (neuroprobe.com)
  • We found that OCLN was located on the LD membrane and was bound to essential regulators of lipid secretion, including BTN1a1 and XOR, in a C-terminus-dependent manner. (bvsalud.org)
  • A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. (lookformedical.com)
  • The TGF-superfamily contains a lot more than 30 proteins ligands split into subfamilies predicated on series similarity and function. (molecularcircuit.com)
  • The adenovirus E1A protein mimics the LxDLFD motif and competes with E2F for binding to Rb, promoting E2F activation and cell proliferation. (eu.org)
  • The retinoblastoma susceptibility gene RB1 was the first tumour suppressor gene to be identified and characterised. (eu.org)
  • The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. (nih.gov)
  • The multifunctional nuclear protein large T-antigen was detectable by immunohistochemical analyses in a subset of neoplastic cells. (cdc.gov)
  • The human Brm protein has been reported to be a 180 kDa nuclear factor that acts as a transcriptional activator when fused to a heterologus DNA binding domain. (bdbiosciences.com)
  • The TREX (transcription/export) complex is conserved in evolution from yeast to man and is required for coupled transcription elongation and nuclear export of mRNAs [ 1 - 4 ]. (biomedcentral.com)
  • Recent data show that Aly and THOC5 function in the tip associating protein (TAP)-p15 mediated nuclear export of Hsp70 mRNA [ 11 ]. (biomedcentral.com)
  • E2F3( AAH16847, 1 a.a. - 134 a.a.) recombinant protein with GST. (novusbio.com)
  • Polyclonal antibody raised in rabbit against the Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein. (diagenode.com)
  • Monoclonal antibody raised in mouse against the N-terminus of the Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein. (diagenode.com)
  • Polyclonal antibody raised in rabbit against Lachnospiraceae bacterium (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein. (diagenode.com)
  • Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein. (diagenode.com)
  • The p63 protein, a homologue of p53, may be associated with tumor formation in the epithelial tissue, acting as an oncogene 11,12 . (bvsalud.org)
  • The cryo-EM analysis also revealed a population of free V 1 bound to Oxidation Resistance 1 (Oxr1p), a poorly characterized protein that has been implicated in protecting cells from oxidative stress. (upstate.edu)
  • shown in red), a poorly characterized protein implicated in oxidative stress response. (upstate.edu)
  • Various physiological and pathological conditions such as glucose starvation, inhibition of protein glycosylation and oxidative stress may cause an accumulation of unfolded proteins in the endoplasmic reticulum (ER), leading to the unfolded protein response (UPR) and autophagy. (spandidos-publications.com)
  • A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
  • In many countries, vaccines against some human papillomavirus (HPV) types are now administered to girls and young women with the goal of protecting them against HPV-induced cervical cancer ( 1 , 2 ). (cdc.gov)
  • Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 Large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. (wikipedia.org)
  • Re-expression of the retinoblastoma-binding protein 2-homolog 1 reveals tumor-suppressive functions in highly metastatic melanoma cells. (wikigenes.org)
  • By means of oligonucleotide microarray and RNA interference, we reveal that the sensitizing effect of IFN-β was possibly due to attenuation of MGMT expression via induction of the protein p53. (aacrjournals.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Rb expression led to phosphorylationof Oct-1, thus relieving its repressive effect. (neuroprobe.com)
  • Consistentwith reduced levels of Oct-1 DNA binding activity in the Rb-transformedcell lines, interleukin 8 expression is higher in these celllines. (neuroprobe.com)
  • Beclin-1 and Atg-5 expression levels were upregulated and LC3 was cleaved after a 6 h purvalanol treatment. (spandidos-publications.com)
  • 1,2 However, recent work has shown that chromatin, the RNA-DNA-protein complex that controls chromosomal organization and gene expression in mammalian nuclei, 3-5 blocks access of Cas9 to certain DNA target sites in vitro 6-8 and in mammalian cells. (aip.org)
  • Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. (embl.de)
  • Zinc-binding motifs are stable structures, and they rarely undergo conformational changes upon binding their target. (embl.de)
  • A recent study from the Wilkens lab in collaboration with Seoul National University uncovered a novel mechanism of yeast V-ATPase regulation based on biochemical experiments and cryo electron microscopy (cryo-EM) structures of holo V-ATPase and Oxr1 bound V 1 subcomplex. (upstate.edu)
  • used cryo-EM to obtain structures of yeast V-ATPase assembled from purified V 1 and V o subcomplexes. (upstate.edu)
  • A histone chaperone protein that plays a role in the deposition of NUCLEOSOMES on newly synthesized DNA . (nih.gov)
  • Residential pesticide exposures in pregnancy and the risk of sporadic retinoblastoma: a report from the Children's Oncology Group. (cdc.gov)
  • and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. (lookformedical.com)
  • Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. (lookformedical.com)
  • The DNA repair protein O 6 -methylguanine-DNA methyltransferase (MGMT) plays an important role in cellular resistance to alkylating agents. (aacrjournals.org)
  • A cellular DNA-repair protein, namely O 6 -methylguanine-DNA methyltransferase (MGMT) protein, reverses alkylation at the O 6 position of guanine, thereby inhibiting the lethal cross-linking and bringing about resistance to alkylating agents ( 2, 3 ). (aacrjournals.org)