• Exons
  • Within the coding sequences of eukaryotes, alternatively spliced exons are known to evolve under relaxed constraints in comparison to those exons that are constitutively spliced because they are not included in all of the mature mRNA of a gene. (ox.ac.uk)
  • There were no other predicted exons within the genomic CXorf26 sequence when 3000 base pairs were added on either side in the search. (wikipedia.org)
  • Except for the signal NH2-terminal peptide, which is absent in the mature enzyme, the amino acid sequence deduced from the exons coincided with the reported primary structure of human liver ALDH2. (wikipedia.org)
  • gene
  • The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene content and genome organization. (nih.gov)
  • The translation of highly repetitive gene sequences is often associated with reduced levels of protein expression and may be prone to mutational events. (pubmedcentralcanada.ca)
  • Development of a new way to make a powerful tool for altering gene sequences should greatly increase the ability of researchers to knock out or otherwise alter the expression of any gene they are studying. (bio-medicine.org)
  • The new method allows investigators to quickly create a large number of TALENs (transcription activator-like effector nucleases), enzymes that target specific DNA sequences and have several advantages over zinc-finger nucleases (ZFNs), which have become a critical tool for investigating gene function and potential gene therapy applications. (bio-medicine.org)
  • TALENs are able to target longer gene sequences than is possible with ZFNs and are significantly easier to construct. (bio-medicine.org)
  • The ability to make a TALEN for any DNA sequence with a high probability of success changes the way we think about gene-altering technology because now the question isn't whether you can target your gene of interest but rather which genes do you want to target and alter. (bio-medicine.org)
  • Very low percentages of sequence coverage and identity of the gene's orthologs in fungi and invertebrates suggest that the gene was lost in those organisms. (wikipedia.org)
  • It contains what is known as the NBPF repeat, which is a two exon stretch of sequence that is characteristic of all 21 members of the NBPF gene family. (wikipedia.org)
  • In this case, artificial gene synthesis is problematic because of improper annealing of the repetitive sequence found in the TALE binding domain. (wikipedia.org)
  • Regions
  • Secondary Structure Adopted by the Gly-Gly-X Repetitive Regions of Dra" by Geoffrey M Gray, Arjan van der Vaart et al. (usf.edu)
  • Low complexity and homopolymer sequences within coding regions are known to evolve rapidly. (ox.ac.uk)
  • Homopolymer sequences are thought to evolve mostly through replication slippage and, therefore, they may be expected to be longer in regions with relaxed selective constraint. (ox.ac.uk)
  • Repetitive structure with high intergenic and intragenic sequence conservation, both in coding and noncoding regions. (wikipedia.org)
  • Apart from the "leucine zipper" and the "basic region" which are important for dimerization and DNA-binding, the c-jun protein contains three short regions, which consist of clusters of negatively charged amino acids in its N-terminal half that are important for transcriptional activation in vivo. (wikipedia.org)
  • specificity
  • To investigate the importance of tryptophan at position 232 (W232) in AvrBs3 and to identify amino acids that broaden or change target specificity for N0 we generated avrBs3 mutant derivatives. (nih.gov)
  • Notably, slight changes in the RVD and the incorporation of "nonconventional" RVD sequences can improve targeting specificity. (wikipedia.org)
  • From these libraries, biochemists can select DARPins to bind the target of choice with picomolar affinity and specificity can be selected using ribosome display or phage display using signal sequences allowing cotranslational secretion. (wikipedia.org)
  • genes
  • We observed a significant relationship between alternative splicing and homopolymer sequences with alternatively spliced genes being enriched in number and length of homopolymer sequences. (ox.ac.uk)
  • TALEN
  • Joung's team also developed a manual version of FLASH that would allow labs without access to robotic equipment to construct up to 24 TALEN sequences a day. (bio-medicine.org)
  • Transcription activator-like effector nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. (wikipedia.org)
  • enzymes
  • Mitochondrial aldehyde dehydrogenase belongs to the aldehyde dehydrogenase family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. (wikipedia.org)
  • Homology directed repair can also introduce foreign DNA at the DSB as the transfected double-stranded sequences are used as templates for the repair enzymes. (wikipedia.org)
  • identical
  • Specifically, degenerate oligonucleotides were used to create a monomer library, which after concatemerization yielded a genetically nonrepetitive DNA sequence that encoded identical pentapeptide repeat sequences. (pubmedcentralcanada.ca)
  • domain
  • Whenever a linear solenoid domain structure participates in protein-protein interactions, frequently at least 3 or more repetitive subunits form the ligand-binding sites. (wikipedia.org)
  • and a transcriptional repression domain (TRD) composed of 104 amino acids The MBD domain forms a wedge and attaches to the methylated CpG sites on the DNA strands. (wikipedia.org)
  • repeat
  • This straightforward relationship between amino acid sequence and DNA recognition has allowed for the engineering of specific DNA-binding domains by selecting a combination of repeat segments containing the appropriate RVDs. (wikipedia.org)
  • fishes
  • When introduced to higher levels of acids, they are able to excrete excess acids at higher rates than most other saltwater fishes, and in much shorter times, with the majority of the transfer of ions occurring at the gill surface. (wikipedia.org)
  • high
  • Sericin has a high hydroxy amino acid content which is important for the water-binding capacity which regulates the skin's moisture content. (wikipedia.org)
  • nuclease
  • Transcription activator-like effectors (TALEs) can be engineered to bind to practically any desired DNA sequence, so when combined with a nuclease, DNA can be cut at specific locations. (wikipedia.org)
  • methods
  • Joung says, "While I believe that TALENs ease of design and better targeting range will probably make them a preferred option over ZFNs made by publicly available methods, ZFNs' smaller size and the less repetitive nature of their amino acid sequences may give them advantages for certain applications. (bio-medicine.org)
  • form
  • This splice form has significantly fewer mRNA base pairs at 977, but still has a protein product of 232 amino acids. (wikipedia.org)
  • Due to the amino acid sequence and the periodicity of the helices, the leucine side chains are arranged along one face of the α helix and form a hydrophobic surface that modulates dimerization. (wikipedia.org)
  • levels
  • As the larvae (called ammocoetes) move towards the oceans, the ratio between saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA) in the gills shifts towards higher amounts of SFA, as they affect the fluidity of the membrane, and higher levels of SFA lead to a decrease in permeability compared to PUFA. (wikipedia.org)