• Quantification
  • For relative quantification of the mRNA using the RT-qPCR technique, the activity of a target gene is expressed in relation to a reference gene (RG) in order to exclude general variations in transcript levels of the cell. (mdpi.com)
  • Among several methods proposed so far [ 1 , 4 ], reference genes are the most frequently used to normalize RT-qPCR data and to control the experimental possible errors generated in the quantification of gene expressions, since the reference genes are exposed to the same preparation steps as the gene of interest. (biomedcentral.com)