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  • Pichia
  • Compared to other eukary otic expression systems, Pichia offers many advantages, because it does not have the endotoxin problem associated with bacteria nor the viral contamination problem of proteins produced in animal cell culture. (bio-medicine.org)
  • Researchers at New Brunswick Scientific (Edison, NJ) found that by switching from a shaker to a fermentor, protein production in Pichia could be increased by over 140% (3). (bio-medicine.org)
  • Antibodies
  • When MBS develops recombinant antigen for a hybridoma development, the end use application of the antibodies is considered during every phase of antigen production. (prweb.com)
  • As a company with rich experience in developing custom antibodies to difficult targets, Maine Biotechnology Services brings the specialized perspective of antibody development professionals to recombinant protein development services. (prweb.com)
  • When protein production is complete, MBS provides a seamless transition to immunizations, shortening the overall timeline to obtain antibodies that meet end use requirements. (prweb.com)
  • antigen
  • Immunogenicity is influenced by multiple characteristics of an antigen: Phylogenetic distance Molecular size Epitope density Chemical composition and heterogeneity Protein structure, aa-polymers, Glu-Lys, Tyr, Phe Degradability (ability to be processed & presented to T cells) D-amino acids In silico screening. (wikipedia.org)
  • vaccine
  • A glycosylated recombinant subunit candidate vaccine consisting of Ehrlichia ruminantium major antigenic protein1 induces specific humoral and Th1 type cell responses in sheep. (abcam.com)
  • Recombinant Proteins are used as antigens in vaccine development to disease study as well as an experimental control for validating research findings to discovering tomorrow's breakthrough. (prosci-inc.com)
  • Fortunately, years of thorough study of the parameters influencing vaccine efficacy allow parallels to be drawn for protein therapeutics. (wikipedia.org)
  • Factors including delivery route, delivery vehicle, dose regimen, aggregation, innate immune system activation, and the ability of the protein to interface with the humoral (B cell) and cellular (T cell) immune systems, all impact the potential immunogenicity of vaccine immunogens when delivered to humans (for reviews related to unwanted immunogenicity determinants, see references below). (wikipedia.org)
  • A vaccine typically contains an agent that resembles a disease-causing microorganism and is often made from weakened or killed forms of the microbe, its toxins, or one of its surface proteins. (wikipedia.org)
  • mammalian
  • Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. (abcam.com)
  • Compounds
  • Ogataea polymorpha with its unusual characteristics provides an excellent platform for the gene technological production of proteins, especially of pharmaceuticals like insulin for treatment of diabetes, hepatitis B vaccines or IFNalpha-2a for the treatment of hepatitis C. Derivatives of both CBS4732 and DL-1 are employed in the production of such recombinant compounds. (wikipedia.org)
  • pastoris
  • A fusion protein composed of a cellulose binding domain from Neocallimastix patriciarum cellulase A and Candida antarctica lipase B (CBD-lipase) was produced by Pichia pastoris methanol utilization plus phenotype in high cell-density cultures. (diva-portal.org)
  • Protein production in E. coli is usually faster than in Pichia pastoris for several reasons: Competent E. coli cells can be stored frozen, and thawed immediately before use, whereas Pichia cells have to be produced immediately before use. (wikipedia.org)
  • coli
  • I'm part of a structural genomics group and we do high though put protein production in E. coli. (bio.net)
  • We describe a statistical model that uses binomial logistic regression for predicting the solubility of heterologous proteins expressed in E. coli . (springer.com)
  • The model is based on a set of proteins reported to have been expressed in E. coli in either soluble or insoluble form. (springer.com)
  • The major advantage of Pichia over E. coli is that Pichia is capable of producing disulfide bonds and glycosylations in proteins. (wikipedia.org)
  • This means that in cases where disulfides are necessary, E. coli might produce a misfolded protein, that is usually inactive or insoluble. (wikipedia.org)
  • production
  • Focusing on the large-scale, cost-effective production of such proteins for use as specialist industrial or therapeutic biomolecules, these authoritative scientists also include promising experimental techniques that will become increasingly important in the future-such techniques as those for the efficient transformation of monocots with Agrobacterium tumefaciens, for optimizing the stability of recombinant proteins, and for exploiting the immunotherapeutic potential of plant-expressed proteins. (springer.com)
  • These strains are being examined for possible uses in biotechnology as host strains for recombinant protein production. (wikipedia.org)
  • Here, the absence of a cell wall can allow production of large amounts of secreted proteins that would otherwise accumulate in the periplasmic space of bacteria. (wikipedia.org)
  • Strains CBS4732 and DL-1 are employed for recombinant protein production, strain NCYC495 is mainly used for the study of nitrate assimilation. (wikipedia.org)
  • It can provide attractive genetic components for an efficient production of proteins. (wikipedia.org)
  • Pichia is widely used for protein production using recombinant DNA techniques. (wikipedia.org)
  • Usually the gene for the desired protein is introduced under the control of the AOX1 promoter, which means that protein production can be induced by the addition of methanol. (wikipedia.org)
  • This is due to E. coli's fast growth rate, good protein production rate and undemanding growth conditions. (wikipedia.org)
  • characteristics
  • Although engineered and modified in a laboratory setting, ELPs share structural characteristics with intrinsically disordered proteins (IDPs) naturally found in the body, such as tropoelastin, from which ELPs were given their name. (wikipedia.org)
  • commercially available
  • These include both commercially available proteins (for example most of the enzymes used in the molecular biology laboratory), and those that are generated in the course specific research projects. (wikipedia.org)
  • Situ
  • With the electron microscopic imaging, FlAsH-EDT2 is also used to study the processes of protein trafficking in situ. (wikipedia.org)
  • Conjugation
  • Since lipids and nucleic acids are non-immunogenic haptens, they require conjugation with an epitope such as a protein or polysaccharide before they can evoke an immunologic response. (wikipedia.org)
  • binds
  • It displays non-specific binding to endogenous cysteine-rich proteins, meaning it binds to sites other than the one of interest (CCXXCC), FlAsH-EDT2 can be prepared in three steps from fluorescein (see figure). (wikipedia.org)
  • Detection
  • In this case, IP was with goat anti HP1-alpha ab77256 on Protein G beads, and detection was with goat anti HP1-alpha ab77256 , and Protein G HRP ab7460. (abcam.com)
  • Novel
  • In the rush to deliver novel biologics to market, developers have, on occasion, overlooked factors that contribute to protein immunogenicity. (wikipedia.org)
  • Cells
  • They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
  • It is used in bioanalytical research as a fluorescent label for visualising proteins in living cells. (wikipedia.org)
  • In the past, FlAsH-EDT2 has been widely used to study a number of in vivo cellular events and subcellular structures in animal cells, Ebola virus matrix protein, and protein misfolding. (wikipedia.org)
  • genetic
  • One approach is to parse protein sequences into overlapping 9-mer peptide frames, each of which is then evaluated for binding potential to each of eight common class II HLA alleles that "cover" the genetic backgrounds of most humans worldwide. (wikipedia.org)
  • Process
  • It is at this temperature that the ELPs move from a linear, relatively disordered state to a more densely aggregated, partially ordered state Although given as a single temperature, Tt, the ELP phase change process generally begins and ends within a temperature range of approximately 2 °C. Also, Tt is altered by the addition of unique proteins to the free ELPs. (wikipedia.org)
  • insoluble
  • In: García-Fruitós E. (eds) Insoluble Proteins. (springer.com)
  • Play media Although ELPs generally form reversible spherical aggregates due to their proline and glycine content, there is a possibility that, under certain conditions such as exceedingly high temperatures, ELPs will form amyloids, or irreversible aggregates of insoluble protein. (wikipedia.org)
  • residues
  • There are two types of bonds involved in a glycoprotein: bonds between the saccharides residues in the glycan and the linkage between the glycan chain and the protein molecule. (wikipedia.org)
  • glycoprotein
  • The nature of N-linked glycans attached to a glycoprotein is determined by the protein and the cell in which it is expressed. (wikipedia.org)
  • This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. (wikipedia.org)
  • sequence
  • On the other hand, the attachment of a glycan residue to a protein requires the recognition of a consensus sequence. (wikipedia.org)
  • The protein also contains type II repeats with epidermal growth factor-like homology and type III repeats that contain an RGD sequence. (wikipedia.org)
  • proven
  • It has been proven to be a good alternative to green fluorescent proteins (GFP) with the advantage that FlAsH-EDT2 is much smaller (molar mass (wikipedia.org)