• vectors
  • The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5' to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell. (patentsencyclopedia.com)
  • vitro
  • Fluorescent protein-based methodologies have revolutionized the field of virology, allowing us to visualize the location and activity of pseudorabies virus (PRV) particles in vitro (G. A. Smith & Enquist, 2002). (princeton.edu)
  • encodes
  • The HMPV fusion (F) protein encodes an RGD (Arg-Gly-Asp) motif that engages RGD-binding integrins as cellular receptors, then mediates fusion of the cell membrane and viral envelope in a pH-independent fashion, likely within endosomes. (wikipedia.org)
  • vivo
  • This product is an active protein and may elicit a biological response in vivo, handle with caution. (abcam.com)
  • It also makes it relatively non-toxic in vivo as the recognized sequence scarcely occurs in proteins. (wikipedia.org)
  • subunit
  • Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. (abcam.com)
  • G saL is the long splice variant of the alpha-subunit of the heterotrimeric G-protein Gs. (abcam.com)
  • mediates
  • We also found that cAMP-GEFII mediates cAMP-dependent, protein kinase A (PKA)-independent insulin secretion, and that this requires interaction with both Rim2 and Piccolo ( 16 - 18 ). (diabetesjournals.org)
  • viral
  • Pneumoviruses replicate entirely in the cytoplasm, and this process begins with adsorption of the virus to the cellular receptor on the host cell, directed by the viral attachment protein, variously called G, H (hemagglutinin) or HN (hemagglutinin-neuraminidase). (wikipedia.org)
  • tumor
  • We can used specific TAAs, tumor lysates,created DC-cancer cell fusions, electroporation/transfection of DCs with total cancer cell-mRNA or tumor derived exosomes (TDEs) by the stimulation.There is also possibility of aditonal co-stimulating with cytokine "cocktails" to assure strong maturation. (wikipedia.org)
  • Antibodies
  • Anti-CD22 immunotoxins, being tested in hairy cell leukemia Antibody-drug conjugates, most, if not all, are immunotoxins but use whole antibodies and non-protein drugs Immunopharmacology and Immunotoxicology (medical journal) Pastan I, Hassan R, FitzGerald DJ, Kreitman RJ (2007). (wikipedia.org)
  • cells
  • Cells in which regulated exocytosis occurs share several molecules that constitute the exocytotic machinery, including SNARE proteins (VAMP/synaptobrevin, SNAP, and syntaxin), synaptotagmins, and Rab proteins ( 2 , 3 ). (diabetesjournals.org)
  • The purified hbFGF proteins, both with and without the fusion tags, were biologically active, which was confirmed by their ability to stimulate proliferation of NIH3T3 cells. (figshare.com)
  • The designer protein combines the binding site for two cytokines that regulate maturation, function, and survival of B cells - B-lymphocyte stimulator (BLyS) and A proliferation-inducing ligand (APRIL), with the constant region of immunoglobin. (wikipedia.org)
  • citation needed] These patients were treated with the recombinant immunotoxin, BL22, which targets the CD22 cell surface receptor, which is highly expressed on these leukemic cells. (wikipedia.org)
  • It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. (wikipedia.org)
  • Interactions
  • HMPV is thought to attach to the target cell via the glycoprotein (G) protein interactions with heparan sulfate and other glycosaminoglycans. (wikipedia.org)
  • active
  • Catalytically active tetramer-dimer (in equilibrium) forms of the fusion protein were separated from inactive, aggregated forms by size-exclusion h.p.l.c. (biochemj.org)
  • activation
  • It is activated by the catecholamine epinephrine and couples to the G protein Gs to mediate adenylate cyclase activation. (abcam.com)
  • Study
  • Included in this study are the first recombinant PRV strains expressing C terminal VP26-mCherry. (princeton.edu)
  • Enhancement of the binding of palivizumab to the HRSV F protein resulted in a second-generation monoclonal antibody, motavizumab (Numax, MedImmune Inc.), which is currently under study in phase III clinical trials. (wikipedia.org)
  • either
  • The properties of this reporter virus, PRV 1028, were compared to previously constructed N terminal recombinants containing either mRFP or mCherry. (princeton.edu)
  • site
  • CAZ ( c ytoskeletal matrix associated with the a ctive z one) proteins have been suggested to organize the site of neurotransmitter release. (diabetesjournals.org)