Pokeweed mitogenConASuccinyl concanavalinAgonistInterleukin-1 receptLectinInsulin receptorsAntibodyStimulationNeutrophilsProgesterone receptorCellsMembranesNuclear receptorLigandMembrane-boundBindHepatitis modelLiverApoptosisAntagonistsImmunoglobulinLymphocytesMurineProteinDerivativeMastReactionsCellEvidenceProliferationBiologyExperimentsInteractionEffect
Pokeweed mitogen1
- While in vitro administration of glucocorticoids may inhibit concanavalin A (Con A)- and phytohemagglutinin (PHA)-induced T-cell proliferation, pokeweed mitogen (PWM)-driven B-cell mitogenesis is relatively resistant to glucocorticoids. (karger.com)
ConA6
- Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally extracted from the jack-bean (Canavalia ensiformis). (wikipedia.org)
- PHA and ConA bind and cross-link components of the T cell receptor, and their ability to activate T cells is dependent on expression of the T cell receptor. (wikipedia.org)
- If ConA is active then agglutination will occur due to ConA's free receptors being able to bind to the glucose residues on the sheep's red blood cells. (studyscroll.com)
- The cells subsequently became resistant to apoptosis when exposed to Fas-specific antibody or when co-cultured with concanavalin A (ConA)-stimulated hepatic mononuclear cells. (the-scientist.com)
- MPZL1 is a main receptor for concanavalin-A (ConA) and it is involved in cellular signaling induced by ConA, which most likely includes Src family tyrosine-protein kinases. (assaygenie.com)
- Although detailed pathophysiological mechanisms of fulminant hepatitis remain elusive, immune cell recruitment with excessive cytokine production is a well-recognized hallmark of the disease.We determined the function of orphan nuclear receptor small heterodimer partner (SHP) in concanavalin A (ConA)-induced hepatitis model. (kribb.re.kr)
Succinyl concanavalin10
- Succinyl-Concanavalin A is a high quality Succinyl-Concanavalin A. This product has been used as molecular tool for various biochemical applications. (moleculardepot.com)
- Differential effects of concanavalin A and succinyl concanavalin A on the macromolecular events of platelet activation Biochim Biophys Acta. (moleculardepot.com)
- 2: Grail A, Norval M. Effect of concanavalin A and succinyl concanavalin A on cytomegalovirus replication in fibroblasts Arch Virol. (moleculardepot.com)
- Effects of bovine serum albumin on the interaction of concanavalin A and succinyl-concanavalin A with phospholipid bilayers Can J Biochem Cell Biol. (moleculardepot.com)
- 5: Garrity P, Szelc C, Paquette C, McEvoy M, Millette R, Adler R. Inhibition of herpes simplex virus replication by succinyl concanavalin A Antimicrob Agents Chemother. (moleculardepot.com)
- Electrokinetic response of granulocytes to concanavalin A and succinyl-concanavalin A Cell Biophys. (moleculardepot.com)
- 7: Marks A, Labourdette G. Succinyl concanavalin A stimulates and antimicrotubular drugs inhibit the synthesis of a brain-specific protein in rat glial cells Proc Natl Acad Sci U S A. 1977 Sep;74(9):3855-8. (moleculardepot.com)
- Interaction with concanavalin A and succinyl-concanavalin A Biochim Biophys Acta. (moleculardepot.com)
- 9: Kido N, Ohta M, Kato N. Inhibition by succinyl concanavalin A of strong adjuvant activity of lipopolysaccharides which possess mannans as the O-specific polysaccharide chains Cell Immunol. (moleculardepot.com)
- Analogues experiments on the mobility of succinyl-concanavalin A receptors yielded similar effects on R, without altering the D value. (tau.ac.il)
Agonist5
- Although both receptors can handle stimulating adenylyl cyclase activity, just the A2aAR is certainly with the capacity of binding the agonist "type":"entrez-protein","attrs":"text":"CGS21680″,"term_id":"878113053″,"term_text":"CGS21680″CGS21680 with high affinity (7,8). (eurodyn2011.org)
- Agonist-induced refractoriness or desensitization is certainly a general feature of G protein-coupled receptors, although just the for 15 min at 4, membranes had been resuspended in a minor level of 50 mM HEPES, 6 pH.8, 10 mM MgCl2 (50/10 buffer), with 0.15 unit/ml ADA, for immediate use in binding assays as defined previously (25). (eurodyn2011.org)
- After treatment Pasireotide with agonist or automobile, monolayers had been rapidly cleaned with ice-cold PBS and scraped into 4 ml of PBS formulated with 0.25 mg/ml concanavalin A to block further receptor redistribution (12). (eurodyn2011.org)
- Here we describe molecular and cellular responses, as well as survival, in A 2A adenosine receptor (AR) agonist treated and untreated animals during experimental sepsis. (biomedcentral.com)
- 2001) A novel viper venom metalloproteinase, alborhagin, is an agonist at the platelet collagen receptor GPVI. (yoproteins.eu)
Interleukin-1 recept2
- The decrease in PWM-generated B-cell proliferation following cortisol depletion by MET may be seen in connection with impaired glucocorticoid-mediated induction of interleukin-1 receptor synthesis. (karger.com)
- We have evaluated the effects of a treatment with soluble interleukin-1 receptor (sIL-1R) in the accelerated model of autoimmune diabetes induced by cyclophosphamide (CY) in the non-obese diabetic (NOD) mouse. (unimol.it)
Lectin4
- Concanavalin A (Con A) completely inhibits IgG-dependent adhesion of human neutrophils, demonstrating lectin-dependent modulation of cell receptor functions. (consensus.app)
- Results are considered to be a manifestation of lectin-dependent modulation of cell receptors functions. (consensus.app)
- The liver injury was induced by injection of concanavalin A (con-A), a lectin derived from jack beans. (biomedcentral.com)
- Cell surface distribution of lectin receptors determined by resonance energy transfer. (uconn.edu)
Insulin receptors2
- Regulation of insulin receptors in frog erythrocytes by insulin and concanavalin A. Evidence for discrete classes of insulin binding sites. (aspetjournals.org)
- They bind to insulin receptors and can imitate insulin (which can prevent fat loss). (impossiblehq.com)
Antibody4
- The early response by lymphocytes to challenge with cell surface-directed ligand, such as antibody or concanavalin A (Con A), involves the clustering of initially diffuse ligand-;receptor complexes into patches, followed by the gathering of these patches into a cap over one region of the cell and their endocytosis 1-;3 . (elsevierpure.com)
- The signaling inhibitory antibody quickly induces ligand-independent receptor clustering and internalization via each caveolin and clathrin-mediated pathways. (kashbiotech.com)
- The non-inhibitory antibody additionally effectively endocytoses through clathrin with out inducing receptor clustering however with slower lysosomal co-localization kinetics. (kashbiotech.com)
- We present that Fc-ARM concentrating on folate receptor-α (FR-α) redirects a clinically used antibody combination to FR-α + most cancers cells, leading to most cancers cell lysis by pure killer cells in vitro . (kashbiotech.com)
Stimulation2
- However, human blood cells, regardless of the multiplicity of the graft, retained their functional reactivity and were able to actively secrete the majority of the analyzed cytokines (8 of 10) in response to stimulation with concanavalin A. Diagnostically significant changes in the concentration of IFN γ , TNF α and IL-4 were detected for the characteristics of a specific antiplague response. (iimmun.ru)
- accordingly, in parallel experiments, splenic lymphoid cells from NOD mice not challenged with CY, but treated with 2 mg/kg sIL-1R for 5 consecutive days showed a normal distribution of mononuclear cell subsets and maintained their capacity to secrete interferon-gamma and IL-2 and to proliferate in response to polyclonal mitogenic stimulation with concanavalin A. (unimol.it)
Neutrophils1
- These abnormalities might be due to abnormal distribution of concanavalin-A receptors on the neutrophils or a cytoskeletal/microtubular abnormality. (medscape.com)
Progesterone receptor1
- The recent cloning and sequencing of a gene coding for a membranous progesterone receptor [ 5 ] in African clawed frog ( Xenopus laevis ) brought evidence for the existence of both intracellular and membrane progestin receptors [ 6 ]. (biomedcentral.com)
Cells8
- Concanavalin A (Con A) covalently linked to Sepharose 4B beads induced localized degranulation of sensitized rat peritoneal mast cells in regions of contact between beads and cells. (rupress.org)
- The finding that sensitized mast cells which had adhered to Con A-Sepharose beads degranulated in regions of the cell away from the area of bead contact if exposed to soluble Con A excluded the possibility that the localized release was due to a redistribution of the IgE receptors or putative Ca2+ channels to the region of bead contact. (rupress.org)
- Using cells of the human lymphoblastoid line WiL2, we have previously shown that receptor-mediated endocytosis occurring at caps involves clathrin-coated vesicles and that the formation of such vesicles is sensitive to drugs such as trifluoperazine dihydrochloride (TFP) that affect calmodulin (CaM), the calcium-dependent regulatory protein of cells 3 . (elsevierpure.com)
- Lymphocytes in the lung-draining lymph nodes were isolated at 3 and 7 days postexposure, analyzed for CD4+ and CD8+ cells, and measured for cytokine production in response to concanavalin A or heat-killed L. monocytogenes. (cdc.gov)
- Cells were then incubated with concanavalin A coupled to CY3 for 3 min before fixation to label the plasma membrane (1:100 dilution in PBS made up of Mg2+ and Ca2+). (researchreportone.com)
- Likewise IFNα2b, Man-HSA(D494N)-IFNα2b caused a significant induction in the mRNA levels of IL-10, IL-1Ra, PD-L1 in RAW264.7 cells and mouse isolated Kupffer cells, and these inductions were largely inhibited by blocking the interferon receptor. (elsevierpure.com)
- As well as, DLL4 ligand binding to the NOTCH3 receptor mediates transendocytosis of NOTCH3-ADCs into ligand-expressing cells. (kashbiotech.com)
- NOTCH3-ADCs internalize into receptor and ligand cells impartial of signaling and induce cell dying in each cell sorts representing an atypical mechanism of ADC cytotoxicity. (kashbiotech.com)
Membranes1
- The concanavalin A receptor from human erythrocytes in lipid bilayer membranes. (moleculardepot.com)
Nuclear receptor1
- Thus, in amphibians, it has been assumed for a long time, that the actinomycin D-insensitive progesterone-induced oocyte maturation [ 4 ] was mediated by a membrane steroid receptor rather than a nuclear receptor. (biomedcentral.com)
Ligand1
- These results are consistent with a functional role for CaM in clathrin recruitment to the cell surface beneath ligand-;receptor complexes and an initial Ca 2+ requiring, microfilament-independent event in receptor-mediated endocytosis. (elsevierpure.com)
Membrane-bound4
- In lower vertebrates, steroid-induced oocyte maturation is considered to involve membrane-bound progestin receptors. (biomedcentral.com)
- Two totally distinct classes of putative membrane-bound progestin receptors have been reported in vertebrates. (biomedcentral.com)
- In addition, we show that both mPR beta and PGMRC1, two members of distinct membrane-bound progestin receptor classes, exhibit highly similar ovarian expression profiles during the reproductive cycle with maximum levels during vitellogenesis and a down-expression during late vitellogenesis. (biomedcentral.com)
- 17. Melchers, F., Andersson, J.: Immunoglobulin production in B-lymphocytes: synthesis of the membrane-bound receptor and the secreted serum glycoprotein immunoglobulin M. Biochem. (uni-mainz.de)
Bind2
- Adenosine can bind to four G protein coupled receptors, A 1 , A 2A , A 2B , and A 3 . (biomedcentral.com)
- Receptor tyrosine kinases that bind COLLAGENS. (bvsalud.org)
Hepatitis model1
- Man-HSA(D494N)-IFNα2b significantly inhibited liver injury in Concanavalin A (Con-A)-induced hepatitis model mice, and consequently improved their survival rate. (elsevierpure.com)
Liver3
- This research is undertaken to evaluate the effect of MSC-derived exosome on concanavalin-A (con-A)-induced liver injury. (biomedcentral.com)
- Recent evidence has shown that in cases of generalized ischemia after hemorrhagic shock, the effectiveness of melatonin in improving liver function depends on the melatonin receptor (40). (ectrx.org)
- Death occurs in mice deficient for Adora2a , the A 2A AR gene, after exposure to Concanavalin A liver injury within 8 hours compared to complete survival in wild-type mice. (biomedcentral.com)
Apoptosis1
- In the February 10 Nature Medicine , Erwei Song and colleagues at Harvard Medical School , Boston, Massachusetts, USA, show that RNA interference (RNAi) targeting the gene Fas (also known as Tnfrsf6, encoding the apoptosis Fas receptor) can protect mice from fulminant hepatitis ( Nature Medicine , DOI:10.1038/nm828, February 10, 2003). (the-scientist.com)
Antagonists1
- These outcomes were reversed by CB1/CB2 antagonists, suggesting that THC-mediated these effects through cannabinoid receptors. (aspetjournals.org)
Immunoglobulin1
- 19. Melchers, F.: Changes in receptor immunoglobulin turnover during B-lymphocyte differentiation. (uni-mainz.de)
Lymphocytes4
- Receptor mobility and receptor-cytoplasmic interactions in lymphocytes. (academicinfluence.com)
- At 7 days postinfection, lymphocytes from Listeria-infected rats showed significant increases in CD4+ and CD8+ cell counts and the CD8+/CD4+ ratio and exhibited increased production of IFN-gamma and IL-2 receptor expression compared with the noninfected control. (cdc.gov)
- incubation of mycobacterium bovis-sensitized bovine peripheral blood lymphocytes with concanavalin a or m. bovis purified protein derivative and indomethacin caused a consistent, statistically significant increase in [3h] thymidine uptake as compared to cultures without indomethacin. (liverpool.ac.uk)
- 27. Melchers, F.: Receptors for the regulation of growth and differentiation of murine B-lymphocytes. (uni-mainz.de)
Murine1
- We noted that murine fetal thymus expressed high levels of the cannabinoid receptors CB1 and CB2. (aspetjournals.org)
Protein2
- topography quality of all G protein-coupled receptors (4, 5). (eurodyn2011.org)
- Myelin Protein Zero-Like 1 (MPZL1) is a cell surface receptor, which is involved in signal transduction processes. (assaygenie.com)
Derivative1
- Capillary electrophoresis analysis of concanavalin A and its succinyl derivative J Pharm Biomed Anal. (moleculardepot.com)
Mast1
- Localized mast cell degranulation induced by concanavalin A-sepharose beads. (rupress.org)
Reactions1
- A mitogen receptor complex which influences reactions leading to proliferation and differentiation. (uni-mainz.de)
Cell2
- To further explore the link between the HPA and the immune system in relation to glucocorticoid receptor function, dose-response curves were obtained for Con A-and PHA-induced T-cell mitogenesis, PWM-generated B-cell mitogenesis and spontaneous lymphocyte proliferation in 13 healthy controls. (karger.com)
- On challenge, concurrent with capping of cell-surface receptors for Con A, CaM localization becomes concentrated in regions of the cytoplasm just below the cap. (elsevierpure.com)
Evidence1
- The process of ischemia and reperfusion is known to cause inducible nitric oxide synthase induction and activation, and there is evidence that interleukin (IL)-1β (46), IL-1 receptor (47), and IL-1 receptor along with nuclear factor-kappa beta (48) may have an important role in that induction. (ectrx.org)
Proliferation1
- These data suggest that the inhibition of spontaneous lymphocyte proliferation by glucocorticoids in vitro is related to glucocorticoid receptor function. (karger.com)
Biology1
- Effect of colchicine and vinblastine on the aggultination of polymorphonuclear leukocytes by Concanavalin A. Nature (New Biology) 238: 120-122. (uconn.edu)
Experiments1
- In other cases such as our mapping of a receptor for small fatty acids, the experiments are complex and require special equipment that has to be constructed and careful analysis of the data is required. (scientificaromatherapy.co.uk)
Interaction1
- 7. Smirnova IV, Khaspekova SG, Ignatov VV, Mazurov AV. (1998) Interaction of wheat germ agglutinin and concanavalin A with platelets. (yoproteins.eu)
Effect1
- Effect of Concanavalin A on phagocytosis. (uconn.edu)