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  • Expression
  • Expression quantitative trait loci in long non-coding RNA ZNRD1-AS1 influence both HBV infection and hepatocellular carcinoma development. (nih.gov)
  • For trait-associated lincRNAs, they also investigated synteny and conservation relative to mouse, expression patterns in five cardiometabolic-relevant tissues, and allele-specific expression in RNA sequencing data for adipose tissue and leukocytes. (lncrnablog.com)
  • long
  • Long intergenic noncoding RNAs (lincRNAs) play important roles in disease, but the vast majority of these transcripts remain uncharacterized. (lncrnablog.com)
  • precursor
  • Also, the small fraction of pulse-labeled nuclear RNA converted to more stable mRNAs, only 5-10%, added to the difficulty of proving a precursor product relationship from pulse-chase labeling experiments. (pnas.org)
  • The synthesis and processing of ribosomal RNAs was much easier to analyze than processing of hnRNAs because about 50% of all nuclear RNA synthesis in growing mammalian cells in culture is synthesis of the single ∼13.7-kb pre-rRNA precursor. (pnas.org)
  • Moreover, this full-length pre-rRNA precursor accumulates to significant levels before it is processed into stable mature rRNAs of 18S (1,870 bases), 5.8S (156 bases), and 28S (5,034 bases), so that the fraction of pre-RNA processed into stable cytoplasmic rRNAs is ∼50%, much more than the 5-10% of pulse-labeled hnRNA processed into mRNAs. (pnas.org)
  • This fortuitous situation for the study of prerRNA by pulse-chase labeling made it possible to show that the rRNAs were processed by the cleavage of the pre-RNA precursor into the stable rRNAs that were transported to the cytoplasm in nearly fully mature ribosomal subunits. (pnas.org)
  • methylation
  • The heterochromation formation in yeast is mediated by RNAi-directed silencing mechanism, while the establishment of DNA methylation in plants is through the RNA-directed DNA methylation (RdDM) pathway. (mdpi.com)
  • mRNAs
  • Pulse and pulse-chase radio-labeling experiments with tritiated uridine had shown that a large fraction of nuclear RNA was degraded very rapidly after synthesis, whereas only a small fraction of the initially synthesized RNA was exported from the nucleus to the cytoplasm where it functions as far more stable mRNAs ( 1 , 2 ). (pnas.org)
  • This short-lived nuclear RNA ranged in lengths up to several tens of kilobases, much longer than most cytoplasmic mRNAs, and was referred to as heterogeneous nuclear RNA (hnRNA) ( 3 , 4 ). (pnas.org)
  • Remarkably, the methyl groups in hnRNA cap structures appeared to be conserved as RNA was exported to the cytoplasm, even though, on average, hnRNA molecules in the nucleus are at least four times longer than mRNAs in the cytoplasm ( 7 , 8 ). (pnas.org)
  • They have been found in several species to be responsible for separating polycistronic transcripts into single gene mRNAs, and in others to splice onto monocistronic transcripts. (wikipedia.org)
  • Some species contain only one spliced leader sequence found on all mRNAs. (wikipedia.org)
  • SMN2
  • An animation shows alternate splicing of the SMN2 gene. (dnalc.org)
  • SOUTH PLAINFIELD, N.J. , Aug. 7, 2014 /PRNewswire/ -- Today the journal Science published results of a preclinical study demonstrating that treatment with orally available RNA splicing modifiers of the SMN2 gene starting early after birth is preventing deficits in mouse models of Spinal Muscular Atrophy (SMA). (bio-medicine.org)
  • Although still preclinical, these results demonstrate how SMN2 splicing modifiers could correct the molecular deficit that causes SMA," said Luca Santarelli , Head of Neuroscience, Ophthalmology and Rare Diseases at Roche. (bio-medicine.org)
  • 2002
  • The luncheon speaker at Splicing 2002 will be UMBI President Dr. Jennie Hunter-Cevera, an innovative leader in biotechnology with extensive background both in academia and industry. (bio-medicine.org)
  • regulation
  • To maintain the pluripotency of the embryonic stem cells, transcription factors and epigenetic modifiers play an important role despite the fact that little is known about the regulation of pluripotency throughout the process of splicing. (wikipedia.org)
  • tumor cell
  • In the new study, Beck and research assistant professor Xiaolong He show that knocking down PTB expression with small, interfering RNA "substantially impairs ovarian tumor cell growth, colony formation and invasiveness. (bio-medicine.org)
  • To the best of our knowledge, this is the first time anyone has shown that interference with the production of a splicing factor can inhibit tumor cell growth and tumor invasiveness," Beck said. (bio-medicine.org)
  • Formation
  • The two reactions are known as transesterification reactions because they require the breaking of one strand of RNA and formation of a new ester linkage. (blogspot.com)
  • Recently, splicing factors are reported to be involved in both RNAi-directed heterochromatin formation in yeast and the RdDM pathway in plants. (mdpi.com)
  • In yeast, splicing factors may provide a platform for facilitating the siRNA generation through an interaction with RDRC and thereby affect the heterochromatin formation, whereas in plants, various splicing factors seem to act at different steps in the RdDM pathway. (mdpi.com)
  • sequence
  • The splice donor site includes an almost invariant sequence GU at the 5' end of the intron, within a larger, less highly conserved region. (wikipedia.org)
  • The splice acceptor site at the 3' end of the intron terminates the intron with an almost invariant AG sequence. (wikipedia.org)
  • However, it is noted that the specific sequence of intronic splicing elements and the number of nucleotides between the branchpoint and the nearest 3' acceptor site affect splice site selection. (wikipedia.org)
  • The relative lack of significant sequence similarity makes splicing a very error-prone reaction. (blogspot.com)
  • Unlike SAGE, detection and purification of transcripts from the 5' end of the messenger RNA require the presence of a trans-spliced leader sequence. (wikipedia.org)
  • post-translationa
  • The first steps of the DDR promote the post-translational modification of splicing factors to affect their localization and activity, while more downstream DDR events alter their expression. (mdpi.com)
  • snRNPs
  • The snRNAs are complimentary to the splice sites and branch sites and that's how the various snRNPs recognize them. (blogspot.com)
  • The spliced RNA is released, the lariat is released and degraded, and the snRNPs are recycled. (wikipedia.org)
  • compounds
  • Analysis of the effects of RBM39 degrading compounds over a panel of 18 AML cells revealed that leukemia cells bearing splicing factor mutations or with high DCAF15 expression were the most sensitive to treatment ( Fig. G ). Genetic introduction of SF3B1, SRSF2, or U2AF1 hotspot mutations in K562 or NALM6 cells resulted in a 20-50% reduction in IC 50 in response to sulfonamides. (confex.com)
  • Cancer
  • A better understanding of how changes in splice site selection are integrated into the DDR may provide new avenues to combat cancer and delay aging. (mdpi.com)
  • For certain types of cancer, like in colorectal and prostate, the number of splicing errors per cancer has been shown to vary greatly between individual cancers, a phenomenon referred to as transcriptome instability. (wikipedia.org)
  • subsequently
  • Poly A messenger RNA is then purified from total RNA and subsequently translated into cDNA using a reverse transcription reaction. (wikipedia.org)
  • factor
  • Researchers in the UIC College of Pharmacy found that interfering with the production of a splicing factor can inhibit the growth and invasiveness of tumor cells in test-tube experiments. (bio-medicine.org)
  • small
  • We understand that we can make small unusual structured drugs called oligonucleotides, which is the same material DNA is made out of by and large, and introduce that into cells and shift the splicing process. (dnalc.org)
  • small RNA guides is transformative for performing systematic gain-of-function studies. (bio-medicine.org)