• polymerase
  • Five micrograms of RNA was reverse transcribed into cDNA and l- μ g aliquots subjected to polymerase chain reaction (PCR) amplification for 25 cycles using 32 P-labeled primers specific for rat cytokines and glyeeraldehyde-3-phosphate dehydrogenase (G3PD), a housekeeping gene. (pubmedcentralcanada.ca)
  • Total RNA
  • Total RNA from 1 X 10(7) cells was extracted, transferred to a membrane, and hybridized with a 32P-labeled, full-length (1650-base pair) rat angiotensinogen complementary DNA (cDNA). (ahajournals.org)
  • Total RNA was extracted from liver grafts using RNAzol (Cinna/Biotecx) and quantitated spectropho-tometrically. (pubmedcentralcanada.ca)
  • genetic
  • We have discovered that substances called ribonucleic acids (RNA), which carry the genetic instructions for the production of telomerase, can be used to overcome this problem and stimulate a strong immune response in cancer patients. (bioportfolio.com)
  • Pseudouridylation of nonsense codons suppresses translation termination both in vitro and in vivo, suggesting that RNA modification may provide a new way to expand the genetic code. (wikipedia.org)
  • mRNAs
  • RNA-binding proteins (RBP) are involved in ( 1 ) splicing and alternative splicing of hnRNA, resulting in the formation of mRNAs. (mdpi.com)
  • mRNAs coexpressed with pleiotropic miRNAs were enriched for RNA metabolism (miR-505-5p), ubiquitin-dependent protein catabolism (miR-197-3p, miR-328) and chromatin assembly (miR-328). (harvard.edu)
  • More than 100 types of RNA modifications have been described so far, recent studies have revealed they are abundant in mRNAs and in regulatory non-coding RNAs (ncRNAs, e.g. lncRNAs, miRNAs, snoRNAs) as well as in tRNAs, rRNAs and snRNAs. (wikipedia.org)
  • tRNA
  • The presence of pseudouridine in the mixed 10S RNA hinted that tmRNA has modified bases found also in tRNA. (wikipedia.org)
  • proteins
  • Given their diverse functions, cells express a variety of RNA-binding proteins, which play important roles in the pathologies of a number of diseases. (mdpi.com)
  • In this review we focus on the effect of alcohol on different RNA-binding proteins and their possible contribution to alcohol-related disorders, and discuss the role of these proteins in the development of neurological diseases and cancer. (mdpi.com)
  • We further discuss the conventional methods and newer techniques that are employed to identify RNA-binding proteins. (mdpi.com)
  • In this review we provide a succinct overview of the role of RNA-binding proteins (RBPs) in regulating gene expression. (mdpi.com)
  • We have divided the review into subheadings under which we discuss the effect of alcohol on RNA-binding proteins, RNA-binding proteins in neurological diseases and cancer, and finally the methods employed to identify RBPs and/or ligands of a RBP. (mdpi.com)
  • For this process the RNA is associated with nuclear proteins that aid in splicing and nuclear export [ 1 ]. (mdpi.com)
  • In trans-translation, tmRNA and its associated proteins bind to bacterial ribosomes which have stalled in the middle of protein biosynthesis, for example when reaching the end of a messenger RNA which has lost its stop codon. (wikipedia.org)
  • bacterial
  • In other bacterial species, a permuted ssrA gene produces a two-piece tmRNA in which two separate RNA chains are joined by base-pairing. (wikipedia.org)
  • Modifications
  • In 2010, He proposed that RNA modifications could be reversible and may have regulatory roles analogous to well-known reversible DNA and protein modifications. (wikipedia.org)
  • Recently, functional experiments have revealed many novel functional roles of RNA modifications. (wikipedia.org)
  • biotech
  • The work is a collaboration between the Verma lab and Arcturus Therapeutics, a local biotech company that developed a system of encapsulating messenger RNA within lipid (fatty acid) nanoparticles. (salk.edu)
  • existence
  • In this second of a two-part clip, Sydney Brenner describes the experiment they did to prove the existence and function of RNA. (dnalc.org)
  • novel
  • A novel database, RMBase (http://mirlab.sysu.edu.cn/rmbase/), has provide various web interfaces to show all RNA modification sites identified from above-mentioned sequencing technologies. (wikipedia.org)
  • cell
  • Presence of angiotensinogen messenger RNA in various cultured cell lines. (ahajournals.org)
  • The transcribed RNA now represents information required to direct cellular function to maintain cell homeostasis. (mdpi.com)
  • production
  • And I said that you could simply act by, the model was that you have some kind of a switch to make the messenger RNA, and that closing or opening the switch at different speed could result in the production at different speed of the protein. (dnalc.org)
  • Sydney Brenner describes what messenger RNA production would look like. (dnalc.org)