• protein
  • The stimulation by methylcholanthrene was inhibited by the simultaneous administration of puromycin of actinomycin D, suggesting that the changes in enzyme level are dependent on protein and RNA synthesis in these tissues. (aacrjournals.org)
  • eIF2A is a 65 kDa protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al. (wikipedia.org)
  • enzyme
  • In enzymology, an O-demethylpuromycin O-methyltransferase (EC 2.1.1.38) is an enzyme that catalyzes the chemical reaction S-adenosyl-L-methionine + O-demethylpuromycin ⇌ {\displaystyle \rightleftharpoons } S-adenosyl-L-homocysteine + puromycin Thus, the two substrates of this enzyme are S-adenosyl methionine and O-demethylpuromycin, whereas its two products are S-adenosylhomocysteine and puromycin. (wikipedia.org)
  • This enzyme participates in puromycin biosynthesis. (wikipedia.org)
  • cells
  • Puromycin acts quickly and can kill up to 99% of nonresistant cells within 2 days. (wikipedia.org)
  • Will selecting edited cells by treatment with Puromycin or Blasticidin increase editing efficiency? (horizondiscovery.com)
  • Puromycin-induced necrosis of crypt cells of the small intestine of mouse. (jax.org)
  • While studying the effect of proteoglycan composition on nephritic permselectivity, it was noted that puromycin treatment of human glomerular endothelial cells (HGEC) altered the sulfation level of GAG chains on proteoglycans such as perlecan, which in turn caused a decrease in the stability of the GAG chains. (wikipedia.org)
  • however
  • Dear all, I am planning to construct a puromycin kill curve for my hepatocellular carcinoma cell lines, however, looking at the protocol that I obtained online, I am not very sure of one of the steps, hopefully someone out there can enlighten me! (protocol-online.org)
  • Site
  • Oligonucleotides and other spacers need to be recruited along with the puromycin to provide flexibility and proper length for the puromycin to enter the A site. (wikipedia.org)
  • Ideally, the linker between the 3' end of an mRNA and the puromycin has to be flexible and long enough to allow the puromycin to enter the A site upon translation of the last codon. (wikipedia.org)
  • library
  • Once the mRNA library is generated, it will be Urea-PAGE purified and ligated using T4 DNA ligase to the DNA spacer linker containing puromycin at the 3' end. (wikipedia.org)
  • The ligated mRNA-DNA-puromycin library is translated in Red Nova Lysate (Novagen) or E. Coli S30 Extract System (Promega), resulting in polypeptides covalently linked in cis to the encoding mRNA. (wikipedia.org)