• This paper, written by Drs. John Wang and Musetta Hanson, is a review of concerns in formulating injectable products containing peptides and proteins. (pda.org)
  • Of these 52 peptides 22 were identified with early disease progression, 5 peptides showed late disease progression, 5 peptides reported a gradual difference in stability over disease progression and 20 peptides indicated no disease progression trend. (duke.edu)
  • KINETIC COMPENSATION EFFECT IN THE HEAT DENATURATION OF WHEY-PROTEIN. (ncsu.edu)
  • ABSTRACT The kinetic compensation effect in the heat denaturation of whey proteins was demonstrated using two different kinetic compensation relationships, i.e. (ncsu.edu)
  • For the study, the authors used this cDNA display proteolysis strategy to systematically come up with about 776,000 high-quality folding stability measurements for hundreds of naturally occurring or engineered protein domains containing single or double amino acid mutations under consistent conditions, for example, making it possible to consider everything from the folding stability contributions of specific amino acids to the interactions affected by protein domain folding stability-related selective pressures. (genomeweb.com)
  • Protein folding is thus the physico-chemical process by which a polypeptidic chain undergoes a structural change from an ensemble of coil like structure up to the unique structure encoded in its amino-acid sequence. (cea.fr)
  • To investigate the relation between protein stability and the predicted stabilities of individual secondary structural elements, residue Pro86 in an alpha-helix in phage T4 lysozyme was replaced by ten different amino acids. (rcsb.org)
  • Unexpectedly, all ten amino acid substitutions marginally reduce protein thermostability. (rcsb.org)
  • This insensitivity of stability to the amino acid at position 86 is not simply explained by statistical and thermodynamic criteria for helical propensity. (rcsb.org)
  • We extracted seven attributes for each record, i.e. the PDB identification code, the protein name, the amino acid mutation, the Gibbs free energy change value (ΔΔG), pH, temperature, and the solvent accessibility of the mutated residue. (lu.se)
  • Analysis of the structure of the CFP-10.ESAT-6 complex and optimized amino acid sequence alignments of M. tuberculosis CFP-10/ESAT-6 family proteins revealed that residues involved in the intramolecular contacts between helices are conserved across the CFP-10/ ESAT-6 family, but not those involved in primarily intermolecular contacts. (kent.ac.uk)
  • Protein is a monomers of amino acids. (biotechfront.com)
  • So we know about amino acids, and these are the monomers that will form proteins, which are also known as polypeptides. (biotechfront.com)
  • Proteins are polymers of amino acids , and they are the most diverse type of biomolecule in your body. (biotechfront.com)
  • And if more than ten come together we will call that a polypeptide , since poly means many and proteins are large polypeptides of around three hundred to a thousand amino acids that are folded in such a way that they have some biological activity. (biotechfront.com)
  • This conformation is a 3D arrangement of atoms and is determined by the sequence of amino acids, Four levels of structures are observed in proteins. (biotechfront.com)
  • Proteins with tertiary structure exhibit folding of their polypeptide chain in aqueous medium (water), where hydrophobic non polar groups of the amino acids are buried interior and hydrophilic polar group remain on the surface. (biotechfront.com)
  • Glow x Grow Proteins are formulated to provide a complete essential amino acids (EAAs) profile, including a potent dose of BCAAs required by the body. (drsu.in)
  • Depending on these findings, we concluded that a tiny and neutral amino acid at the 64th position is essential for the stability with the ZIP13 protein. (cathepsin-s.com)
  • The replacement of G64 with an amino acid having a big or standard side chain caused its protein level to decrease, and acidity in the 64th position was fatal for the ZIP13 protein, major to its clearance by the proteasome-dependent (20S proteasome-independent: Supplementary Fig S5) degradation pathway. (cathepsin-s.com)
  • ments of the amino acids along the protein chains. (lu.se)
  • Our analysis has been the amino acid sequences in proteins differ from what is carried out using two different methods, which differ substantially expected from random sequences in a statistically significant from what is used in ref. 3, although the starting point is similar. (lu.se)
  • PROT data base (6) of functional proteins, this method yields model containing only two amino acid types, hydrophobic and clear evidence for nonrandomness. (lu.se)
  • To under- denoted the AB model, consists of chains of two kinds of stand the statistical distribution of hydrophobicity along proteins ``amino acids'' interacting with Lennard-Jones potentials. (lu.se)
  • Recent evidence indicates that altered degradation rate of mutant proteins represents a pathogenic mechanism underlying genetic diseases. (frontiersin.org)
  • Similarly, for p53 to function as a tumor suppressor gene, it also needs to get rid of MDM2-mediated degradation, so as to stabilize the p53 protein to function. (medicaltrend.org)
  • Cell-fate-determinant molecule NUMB-interacting protein (TBC1D15) is overexpressed and contributes to p53 degradation in TICs. (nature.com)
  • One of the PD mouse models involved characterizing the protein interactions induced by mutated leuceine-rich repeat kinase 2 (LRRK2) at a pre-symptomatic time point (3 months old). (duke.edu)
  • Furthermore, these results establish a new biophysical link between the hit proteins identified and their role in aging, LRRK2 protein interactions, and PD progression. (duke.edu)
  • These proteins have two or more polypeptide subunits joined by covalent links (disulfide bonding) or non-covalent forces such as ionic forces, hydrogen bonding or hydrophobic interactions. (biotechfront.com)
  • In this new follow-up project we investigate methods to quantify/characterize protein-protein interactions in complex dairy protein mixtures (as such, mixed with hydrolysates, and mixed with plant proteins). (topsectoragrifood.nl)
  • 2. Role of protein-protein interactions and aggregation on protein digestion kinetics. (topsectoragrifood.nl)
  • Therefore we will aim at quantifying protein-protein interactions and aggregation by various experimental techniques in combination with advanced multiscale modelling techniques. (topsectoragrifood.nl)
  • Protein-water interactions have long been recognized as a major determinant of chain folding, conformational stability, binding specificity and catalysis. (huji.ac.il)
  • However, the detailed effects of water on stabilizing protein-protein interactions remain elusive. (huji.ac.il)
  • Seven such interactions within the interface between TEM1 and BLIP proteins were evaluated. (huji.ac.il)
  • Monitor the hydrodynamic properties of biomacromolecules and protein-protein interactions. (utmb.edu)
  • This information can be used to analyze protein-protein interactions, the presence of oligomeric states, and conformational rearrangement of molecules. (utmb.edu)
  • pleated sheets provide strength and rigidity to structural proteins. (biotechfront.com)
  • Hence, any substantial structural perturbation due to salt, pH, alcohol, or heat treatment leads to irreversible changes in protein structure, even if a simulated physiological environment is restored. (ncsu.edu)
  • The structural complexity of these molecules requires careful handling to ensure integrity and stability of the drug. (lu.se)
  • Do Protein Structural Features Confer Stability to Digestion of Food Allergens? (usda.gov)
  • Solution molecular biophysics and thermodynamic techniques are used both as a primary research tool as well as means to structural studies (for example in the exploration of conditions for crystallization of proteins). (utmb.edu)
  • The ability of a protein to retain its structural conformation or its activity when subjected to physical or chemical manipulations. (bvsalud.org)
  • The observed conformational changes illustrate a general mechanism by which proteins can tolerate mutations. (rcsb.org)
  • 2002). The criteria for the data selection were as follows: (1) only single point mutations, (2) the protein structure is available in Protein Data Bank. (lu.se)
  • Finally, we had 2760 mutations affecting 75 proteins, with 1887 negative and 873 positive cases, respectively. (lu.se)
  • This dataset contained 1810 mutations affecting 71 proteins, with 1388 negative and 422 positive examples, respectively. (lu.se)
  • constructed a dataset from ProTherm database, containing 1948 mutations affecting 58 proteins with 1154 positive cases (Capriotti, et al. (lu.se)
  • S388 contained 388 single mutations in 17 proteins (Capriotti, et al. (lu.se)
  • Phenotypic severity of homozygous GCK mutations causing neonatal or childhood-onset diabetes is primarily mediated through effects on protein stability. (ox.ac.uk)
  • We report the largest case series of homozygous GCK mutations to date and demonstrate that they can cause childhood-onset diabetes, with protein instability being the major determinant of mutation severity. (ox.ac.uk)
  • RPSA gene mutations are thought to reduce the amount of functional ribosomal protein SA. (medlineplus.gov)
  • A shortage of the normal protein likely impairs the assembly of ribosomes, but the specific effects of the mutations are not known. (medlineplus.gov)
  • The mutation responsible for the inherited form of ALS is known to originate in the gene encoding the TDP-43 protein, but the relationship between the biochemical properties of TDP-43 and the progression of ALS has been unclear. (medicalxpress.com)
  • The second PD mouse model studied was one in which the human α-synuclein protein, containing the known PD mutation A53T, was overexpressed. (duke.edu)
  • The protein has the mutation C127S to increase its stability. (giottobiotech.com)
  • The protein has been mutated to increase its stability, as the mutation drastically reduces the enzyme's rate of autoproteolysis. (giottobiotech.com)
  • However, finding a generic method that is applicable to membrane proteins has been a challenge as the high hydrophobicity of membrane proteins and the presence of detergents essential for their solubilization interfere with fluorescence-based detections. (nih.gov)
  • Here the authors used MsbA (an adenosine triphosphate binding cassette transporter), CorA (a Mg(++) channel), and CpxA (a histidine kinase) as model proteins and show that DSLS is not sensitive to the presence of detergents or protein hydrophobicity and can be used to monitor thermodenaturation of membrane proteins, assess their stability, and detect ligand binding in a 384-well format. (nih.gov)
  • This approach demonstrated that neither Rv0287.Rv0288 nor the CFP-10.ESAT-6 complexes are destabilized at low pH (4.5), indicating that even in low pH environments, such as the mature phagosome, both Rv0287.Rv0288 and CFP-10.ESAT-6 undoubtedly function as complexes rather than individual proteins. (kent.ac.uk)
  • This analysis identified the molecular basis for the specificity and stability of complex formation between CFP-10/ ESAT-6 family proteins, and indicates that the formation of functional complexes with key roles in pathogenesis will be limited to genome partners, or very closely related family members, such as Rv0287/Rv0288 and Rv3019c/Rv3020c. (kent.ac.uk)
  • Jannik Bjerrum (son of Niels Bjerrum) developed the first general method for the determination of stability constants of metal-ammine complexes in 1941. (wikipedia.org)
  • Bjerrum went on to determine the stability constants for systems in which many complexes may be formed. (wikipedia.org)
  • These findings represent an essential step towards optimizing protein dynamics studies on large complexes or measuring features that require high forces, long-term assessments, and simultaneous imaging. (lumicks.com)
  • For more information, you can read the full article entitled " Simultaneous sensing and imaging of individual biomolecular complexes enabled by modular DNA-protein coupling " in the journal Communications Chemistry . (lumicks.com)
  • These complexes interact with 5'-AMP-activated protein kinase (AMPK) that is part of the mechanistic target of rapamycin (mTOR) pathway, which is involved in cellular energy, cytokinesis, cell motility, cellular adhesion, and nutrient sensing. (medscape.com)
  • Protein stabilization upon ligand binding has frequently been used to identify ligands for soluble proteins. (nih.gov)
  • Our Surface Plasmon Resonance (SPR) Biacore T100 biosensor instrument is a high-throughput equipment that allows the study of protein-protein and protein-ligand kinetics and thermodynamics. (utmb.edu)
  • Two recombinant phage, HJ1 and HJ4, containing homologous inserts of 350 and 600 bp, respectively, produced proteins that reacted with antibody. (ncsu.edu)
  • Recombinant mutant GCK proteins were analyzed for kinetic and thermostability characteristics and assigned a relative activity index (RAI) or relative stability index (RSI) value. (ox.ac.uk)
  • Comparison with simulations on bacterial proteins suggests that the effect of cholesterol is much less pronounced for the latter than for the mouse protein. (preprints.org)
  • New research by Koji Yamanaka and colleagues from the Laboratory for Motor Neuron Disease at the RIKEN Brain Science Institute has now revealed that increased stability of mutant TDP-43 is associated with earlier onset of ALS. (medicalxpress.com)
  • This increased stability also made the mutant TDP-43 molecules more toxic to the cells. (medicalxpress.com)
  • Accelerated disease onset with stabilized familial Amyotrophic Lateral Sclerosis (ALS)-linked mutant TDP-43 proteins. (medicalxpress.com)
  • Constant with this discovering, cotreatment with PYR-41 (a ubiquitinactivating enzyme E1 inhibitor) along with the protein synthesis inhibitor cyclohexamide (CHX) suppressed the lower in mutant ZIP13 protein expression in HeLa cells (Fig 4A). (cathepsin-s.com)
  • In Nature , a team from the US, Japan, France, and Israel outlines a strategy for measuring thermodynamic folding stability across 'mega-scale' protein domain sets. (genomeweb.com)
  • However, four protein hits, dihydropyrimidinase-related protein 2, eukaryotic translation initiation factor 4A2, Rap1 GTP-GDP dissociation stimulator 1 and myelin basic protein, were identified with consistent thermodynamic stability in multiple mice within a biological state and as hits in multiple comparisons suggesting they are the most likely to be true positives. (duke.edu)
  • When compared to similar techniques like isothermal titration calorimetry (ITC) it has reduced protein consumption. (utmb.edu)
  • Using mouse embryonic stem cells as a model, we show that Prmt5 regulates mRNA binding proteins that are enriched in intrinsically disordered regions and involved in liquid-liquid phase separation mechanisms, including the formation of stress granules. (gencat.cat)
  • A balance between elongation and trimming regulates telomere stability in stem cells. (sens.org)
  • For sequences with a typical fraction of hydrophobic residues, we impact on how permissive with respect to sequence specificity find that the nonrandomness can be interpreted as anticorrela- the protein folding process is-- only sequences with nonran- tions. (lu.se)
  • This involves a large conformational change in residues 81 to 83 and smaller shifts that extend 20 angstroms across the protein surface. (rcsb.org)
  • It specifically cleaves before lysine and arginine residues in proteins. (sigmaaldrich.com)
  • Thus, buried waters have an important role in stabilizing the monomer fold rather than contributing to protein-protein binding, and are not replaced by residues from the incoming protein. (huji.ac.il)
  • Following the synthesis by the ribosome, to carry out its biological function, a protein much fold into a single, well defined conformational state: the native state. (cea.fr)
  • Proteins having more than one polypeptide chains exhibit fourth level of protein structure, called quaternary structure. (biotechfront.com)
  • Swaisgood proposed the principle of physical isolation of nascent folding polypeptide chains and has shown that even proteins that cannot be refolded in solution to give biologically active structure can be refolded to the native state if the folding molecules are physically isolated by immobilization. (ncsu.edu)
  • Endogenous Trol GFP strain produces a functional Perlecan protein. (elifesciences.org)
  • Recently, thermal stability analyses have been developed which provide a proxy of a protein functional status. (gencat.cat)
  • Here, we show that molecular and functional events closely linked to protein methylation can be revealed by the analysis of thermal stability. (gencat.cat)
  • functional proteins. (lu.se)
  • As PPSC is trained with protein structures, PDB file has to be downloaded. (lu.se)
  • These structures are found in fibrous, linear or rod shaped proteins, where the peptide chain shows a regular helical conformation. (biotechfront.com)
  • The nonnative structures thus formed have properties that are different from those of the native protein. (ncsu.edu)
  • These proteins come together to form structures called ribosomes. (medlineplus.gov)
  • The Jasco J-815 Circular Dichroism Spectrometer housed in the SBL allows for the quick and easy determination of secondary and tertiary structures of protein samples. (utmb.edu)
  • This model, hereafter formation of three-dimensional protein structures. (lu.se)
  • They also found that stabilized TDP-43 protein loses the ability to control its own mRNA transcripts, thereby further accelerating its accumulation. (medicalxpress.com)
  • mRNA contains the chemical instructions that ribosomes, the protein-making machinery in cells, use to make proteins. (cdc.gov)
  • Using the genetic code of the SARS-CoV-2 virus, vaccine manufacturers developed mRNA that instructs ribosomes to produce the spike protein of the SARS-CoV-2 virus. (cdc.gov)
  • In comparison, mRNA vaccines can be more quickly generated in the laboratory using the genetic sequences for selected pathogen proteins. (cdc.gov)
  • For vaccine mRNA to deliver the instructions for making the spike protein, it needs to be able to enter the cells so that the protein-making machinery can access it. (cdc.gov)
  • 300 enzymes, and in the metabolism of proteins and nucleic acids (WHO 2001). (cdc.gov)
  • UTMB also pioneered the use of osmolytes in the stabilization of intrinsically unstructured regions of proteins ( 2 ) and the SBL has a long tradition of the use of protecting osmolytes in biophysical studies ( 3 ). (utmb.edu)
  • Deepened knowledge on the biophysical chemistry of proteins with emphasis on properties rather than methods. (lu.se)
  • Rossetti, G. Impact of Cholesterol on the Stability of Monomeric and Dimeric Forms of the Translocator Protein TSPO: A Molecular Simulation Study. (preprints.org)
  • Here, we present a coarse-grained molecular dynamics study on the mouse protein in the presence of a physiological content and of an excess of cholesterol. (preprints.org)
  • The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 18.8 and 25.0 kDa. (giottobiotech.com)
  • This will give important generic scientific insights in the relation between protein molecular properties and aggregation propensity and dynamics as well as on the role of other non-protein components. (topsectoragrifood.nl)
  • Yang Yang, Biao Chen, Ge Tan, Mauno Vihinen, and Bairong Shen, 2013,Structure-based prediction of the effects of a missense variant on protein stability. (lu.se)
  • permissive with respect to sequence specificity the protein folding process is, we have carried out the same analysis for a Section 1: Introduction toy model (7, 8), for which unbiased samples of folding and Hydrophobicity is widely believed to play a central role in the nonfolding sequences can be obtained. (lu.se)
  • PC-1 is a cardiomyocyte mechanosensor that is required for cardiac hypertrophy through a mechanism that involves stabilization of α1C protein . (bvsalud.org)
  • The native three dimensional conformation of proteins maintained by influence of different types of covalent and non-covalent bonds. (biotechfront.com)
  • Intrinsically disordered proteins and their contraction and folding can be monitored using this technique. (utmb.edu)
  • The biological variability of the protein stability measurements was low, and within the experimental error of the SPROX technique. (duke.edu)
  • This product is an active protein and may elicit a biological response in vivo, handle with caution. (abcam.com)
  • The study is a further step towards improving protein tethering that requires long and robust DNA handles while simultaneously observing protein dynamics. (lumicks.com)
  • These methods will allow investigation of the role of aggregation and its dynamics in relation to storage stability and digestibility. (topsectoragrifood.nl)
  • The protein molecules, under influence of two types of chemical sources form secondary structure. (biotechfront.com)
  • Here, we report that telomere length is determined by the balance between telomere elongation, which is mediated by telomerase, and telomere trimming, which is controlled by XRCC3 and Nbs1, homologous recombination proteins that generate single-stranded C-rich telomeric DNA and double-stranded telomeric circular DNA (T-circles), respectively. (sens.org)
  • Edible films based on low (LSA) and high (HSA) viscosity sodium alginate, low esterified amidated pectin (PEC), kappa-carrageenan/locust bean gum (κ-CAR/LBG) and gelatine (GEL) in the presence or absence of whey protein concentrate (WPC) were shown to be feasible carriers for the delivery of L. rhamnosus GG. (nottingham.ac.uk)
  • The effect of dialysis on shear stress and shear strain at failure of whey protein concentrate/isolate gels. (ncsu.edu)
  • Yamanaka's group found that stabilized TDP-43 protein molecules inhibit proteasome activity, thus adding to the growing body of evidence that this clearing mechanism fails in neurodegenerative diseases . (medicalxpress.com)
  • Proteins are very large compared to simple molecules. (biotechfront.com)
  • Protein molecules, under influence of additional chemical forces, other than that of covalent boniding and hydrogen bonding show tertiary structure. (biotechfront.com)
  • Compared with the previous yeast display proteolysis method, cDNA display proteolysis resolves a wider dynamic range of stability and is more reproducible even at a 50-fold larger experimental scale. (genomeweb.com)
  • We provide a comprehensive review of the available experimental data on the single molecule characterisation of proteins using the atomic force microscope. (whiterose.ac.uk)
  • Prediction of Protein Stability Changes (PPSC) is based on M8 and M47 models developed to utilize 8 and 47 features, respectively, in a support vector machine optimized with experimental data. (lu.se)
  • Taking together the experimental and bioinformatics evidence suggests that exposed waters within the interface may be good sites for protein engineering, while buried or mostly buried waters should be left unchanged. (huji.ac.il)
  • Using their high-throughput complementary DNA (cDNA) display proteolysis assay, the investigators report they can gauge protein folding stability patterns in a library of up to 900,000 sequences per week with a reagent price tag of around $2,000. (genomeweb.com)
  • On the basis of products, the protein stability analysis market is divided into reagents & assay kits, instruments, consumables & accessories, and software. (marketsandmarkets.com)
  • The large share of this market segment can be attributed to the high consumption of reagents and assay kits in protein stability analysis techniques, increasing protein pharmaceutical R&D, and increased government funding for life science research. (marketsandmarkets.com)
  • This provides evidence that SOD1 stability and its aggregation propensity are the main toxic causes of ALS. (genengnews.com)
  • LysargiNase is suitable for peptide or protein digestion in solution or in gel. (sigmaaldrich.com)
  • For peptide or protein digestion, a ratio of between 1:100 to 1:20 (w/w) enzyme:substrate is recommended. (sigmaaldrich.com)
  • HJ1 and HJ4 lysogens produced beta-galactosidase fusion proteins that shared similar epitopes with each other and helveticin J. The intact helveticin J gene (hlv) was isolated by screening a library of L. helveticus chromosomal DNA in lambda EMBL3 with the insert DNA from phage HJ4 as a probe. (ncsu.edu)
  • The traditional theory is that when cells are stimulated, the binding of MDM2 and p53 will be destroyed, leading to the accumulation of p53 protein, which affects downstream gene expression and enables cells to respond to the stimulus. (medicaltrend.org)
  • The RPSA gene provides instructions for making a protein called ribosomal protein SA, which is one of approximately 80 different ribosomal proteins. (medlineplus.gov)
  • Research suggests that ribosomal protein SA helps the ribosome control the production of certain proteins, many of which are likely important for development before birth. (medlineplus.gov)
  • Proteins with tertiary structure have a three dimensional structure. (biotechfront.com)
  • The effect of heat on the stability of both monomers and oligomers was investigated. (nih.gov)
  • ZIP13G64D protein is readily degraded by a proteasome-dependent mechanism. (cathepsin-s.com)
  • In coordination chemistry, a stability constant (also called formation constant or binding constant) is an equilibrium constant for the formation of a complex in solution. (wikipedia.org)
  • A recent study in Communications Chemistry shows how a research lab at AMOLF in the Netherlands optimized DNA-protein coupling to study dynamic processes requiring high forces and long-term assessments. (lumicks.com)
  • Lactobacillus helveticus 481 produces a 37-kDa bacteriocin called helveticin J. Libraries of chromosomal DNA from L. helveticus were prepared in lambda gt11 and probed for phage-producing fusion proteins that could react with polyclonal helveticin J antibody. (ncsu.edu)
  • HeLa cells stably expressing the indicated proteins were treated with proteasome inhibitors ten lM MG132 or 1 lM lactacystin for six h, followed by Western blot of whole-cell lysates applying an anti-V5 antibody. (cathepsin-s.com)
  • This new method showed an acceptable stability and reproducibility and was validated with OP-AChE spiked human plasma. (cdc.gov)
  • 111 Pages Report] The market for protein stability analysis is expected to grow from USD 1.2 billion in 2018 to USD 2.1 billion by 2024, at a CAGR of 10.0 % during the forecast period. (marketsandmarkets.com)
  • Neurodegenerative diseases are characterized by the aggregation of misfolded proteins, which accumulate to form insoluble clumps within or around nerve cells. (medicalxpress.com)
  • Comparative analyses on thermodynamic stability profiles of ~470 proteins revealed relatively few differences. (duke.edu)
  • The relative stabilities of these proteins were assayed in a series of comparative analyses involving mice at the different time points and the normally aged mice from above. (duke.edu)
  • However, systems analyses of protein methylation lag behind compared to other modifications. (gencat.cat)
  • Our approach provides an opportunity to systematically explore protein methylation function and represents a rich resource for understanding its role in pluripotency. (gencat.cat)
  • They then introduced the mutated genes into neurons growing in culture dishes in order to induce the cells to synthesize the mutated proteins. (medicalxpress.com)
  • What is interesting is that although these mice showed activation of p53 (for example, the up-regulation of p53 target genes was observed in different tissues), the content of p53 protein did not increase significantly. (medicaltrend.org)
  • Methods such as differential scanning fluorimetry (DSF) and differential static light scattering (DSLS) have been employed in the 384-well format and have been useful in identifying ligands that promote crystallization and 3D structure determination of proteins. (nih.gov)
  • A sensitive magnetic nanoparticle-based immunoassay of phosphorylated acetylcholinesterase using protein cage templated lead phosphate for signal amplification with graphite furnace atomic absorption spectrometry detection. (cdc.gov)
  • We developed a new magnetic nanoparticle sandwich-like immunoassay using protein cage nanoparticles (PCN) for signal amplification together with graphite furnace atomic absorption spectrometry (GFAAS) for the quantification of an organophosphorylated acetylcholinesterase adduct (OP-AChE), the biomarker of exposure to organophosphate pesticides (OPs) and nerve agents. (cdc.gov)
  • Compared with mass spectrometry-based high-throughput stability assays, cDNA display proteolysis achieves a 100-fold larger scale and can easily be applied to study mutational libraries that pose difficulties for proteomics," the authors report. (genomeweb.com)
  • Owusu, RK & Cowan, DA 1989, ' Thermostable microbial protein stability in aqueous: organic two-solvent phase systems ', Biochemical Society Transactions , vol. 17, pp. 581-582. (ulster.ac.uk)
  • Different kinds of proteins include enzymes that catalyze chemical reactions, receptors that control signaling in your body, hemoglobin, which carries oxygen throughout the bloodstream, muscle and organ tissue, which gives your body structure and mobility, and so many other things. (biotechfront.com)
  • The two equilibria are H + L ⇋ H L {\displaystyle \mathrm {H+L} \leftrightharpoons \mathrm {HL} } M + L ⇋ M L {\displaystyle \mathrm {M+L} \leftrightharpoons \mathrm {ML} } Hence by following the hydrogen ion concentration during a titration of a mixture of M and HL with base, and knowing the acid dissociation constant of HL, the stability constant for the formation of ML could be determined. (wikipedia.org)
  • Use the literature and databases to improve the scientific level of protein research projects. (lu.se)