• stable
  • In a pioneering study by McLafferty and coworkers, gas-phase HDX was used to provide some of the first experimental evidence for stable coexisting protein gas-phase conformations 15 . (pubmedcentralcanada.ca)
  • This configuration is equivalent to a stable, globally optimized potential energy state of the system or, in other words, the final conformation of the protein. (asme.org)
  • Recent emergence of oseltamivir and zanamivir resistant human influenza A(H1N1) H274Y has emphasized the need for suitable expression systems to obtain large quantities of highly pure and stable, recombinant neuraminidase through two separate artificial tetramerization domains that facilitate the formation of catalytically active neuraminidase homotetramers from yeast and Staphylothermus marinus, which allow for secretion of FLAG-tagged proteins and further purification. (wikipedia.org)
  • methodology
  • This paper presents an efficient and novel computational protein prediction methodology called kineto-static compliance method. (asme.org)
  • Successive kineto-static fold compliance is a methodology for predicting a protein molecule's motion under the effect of an inter-atomic force field without the need for molecular-dynamic simulation. (asme.org)
  • Immune
  • Our results demonstrate that the conformation of antigens in VLPs is of critical importance for optimal stimulation of protective as well as durable immune responses. (umassmed.edu)
  • scrapie
  • The protein can exist in multiple isoforms, the normal PrPC, and as Protease resistant PrPRes like the disease-causing PrPSc(scrapie) and an isoform located in mitochondria. (wikipedia.org)
  • GPCRs
  • However, various GPCRs differ sufficiently in the tilts of the TMHs that this method need not predict the optimum conformation starting from any other template. (pnas.org)
  • G-protein-coupled receptors (GPCRs) [also referred to as seven-transmembrane receptors (7TMRs)] are integral membrane proteins that play a central role in transmembrane (TM) signal transduction. (pnas.org)
  • G protein-coupled receptors (GPCRs) traverse the plasma membrane seven times and produce intracellular effects through interaction with G proteins. (sciencemag.org)
  • This suggests that inverse agonists are not merely "the opposite of agonists," but instead may serve as useful tools to investigate ligand-specific conformations of GPCRs. (sciencemag.org)
  • computational
  • Levitt was a PhD student in Computational biology at Peterhouse, Cambridge, and was based at the Laboratory of Molecular Biology from 1968 to 1972, where he developed a computer program for studying the conformations of molecules that underpinned much of his later work. (wikipedia.org)
  • Distinct
  • It is known that G-protein-coupled receptors exhibit several distinct low-energy conformations, each of which might favor binding to different ligands and/or lead to different downstream functions. (pnas.org)
  • aggregation
  • This information can be relevant for understanding important areas of biology such as protein folding, protein aggregation and amyloid formation. (pubmedcentralcanada.ca)
  • Tanaka, M., Machida, Y., and Nukina, N. A novel therapeutic strategy for polyglutamine diseases by stabilizing aggregation-prone proteins with small molecules. (riken.jp)
  • sequence
  • 44 prolines were found along the protein, making up 12.3% of the total amino acid sequence. (wikipedia.org)
  • A cross-program consensus predicted that UPF0575 protein C19orf67 forms four alpha helices and two beta sheets in the following locations in the amino acid sequence: Acetylation is likely to occur at the N-terminus while the C-terminus is unlikely to be modified. (wikipedia.org)
  • interaction
  • Fluorinated C3-benzamido substituents induced a shift in the side-chain conformation of R144 to allow for an entropically favored electrostatic interaction between its guanidine group and the 2- O -sulfate of the ligand. (rsc.org)
  • Disease
  • Neame, P. J. and Gallagher, J. T. (2005) Extracellular Matrix Heparan Sulfate Proteoglycans, in Amyloid Proteins: The Beta Sheet Conformation and Disease (ed J. D. Sipe), Wiley-VCH Verlag GmbH, Weinheim, Germany. (wiley.com)
  • An alpha-synuclein fragment, known as the non-Abeta component (NAC) of Alzheimer's disease amyloid, originally found in an amyloid-enriched fraction, was shown to be a fragment of its precursor protein, NACP. (wikipedia.org)
  • function
  • Understanding the function of such proteins requires knowledge of the ensemble of low-energy configurations that might play a role in this pleiotropic functionality. (pnas.org)
  • Where it is expressed, the protein is predicted to be localized in the nucleus to carry out a function. (wikipedia.org)
  • The highly conserved and slowly evolving DUFF3314 region is predicted to form numerous alpha helices and may be vital to the function of the protein. (wikipedia.org)
  • Although the function is not yet well understood, conservation among numerous taxa indicate that it may be important to the function of the protein. (wikipedia.org)
  • Alpha-synuclein is a synuclein protein of unknown function primarily found in neural tissue, making up as much as 1% of all proteins in the cytosol of brain cells. (wikipedia.org)
  • In this field, the main interest is on proteins, because discovering their three-dimensional conformation allows us to get clues about the function they are able to perform. (wikipedia.org)
  • abundant
  • The proline content by percentage was found to be higher in UPF0575 than 95% of comparable human proteins.However, the amount of asparagine the protein is less abundant when compared to the human proteome. (wikipedia.org)
  • The protein product is less abundant than most of the human proteome in many tissues. (wikipedia.org)
  • Alpha-synuclein is a protein that is abundant in the human brain. (wikipedia.org)
  • Atoms
  • Experimental techniques are able to estimate distances between pairs of atoms of a given molecule, and the problem becomes the one of identifying the three-dimensional conformation of the molecule, i.e. the positions of all its atoms. (wikipedia.org)
  • small
  • In this paper, we have used PROTOFOLD to predict the final conformation of a small peptide chain segment, an alpha helix, and the Triponin protein chains from a denatured configuration. (asme.org)
  • gene
  • The spliceosome: NMR spFRET dice up protein complex spectroscopyNOW.com Cells convert DNA to RNA to template protein production but before that final step, gene expression, the non-coding regions of the genome have to be removed by the so-called spliceosome. (bionmr.com)
  • UPF0575 protein C19orf67 is a protein which in humans (Homo sapiens) is encoded by the C19orf67 gene. (wikipedia.org)
  • The DUF3314 region of the gene appears to have diverged at a slower rate relative to the rest of the gene, indicating that that region may have been undergoing purifying selection because that region played an important role in the protein. (wikipedia.org)
  • helix
  • Other helices, such as the 310 helix and π helix, are calculated to have energetically favorable hydrogen-bonding patterns but are rarely observed in natural proteins except at the ends of α helices due to unfavorable backbone packing in the center of the helix. (wikipedia.org)