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  • primers
  • The method uses a primary PCR amplification reaction with a universal reverse primer and two forward primers specific for the genus Plasmodium and to mammals (the mammalian-specific primer was included as a positive control to distinguish uninfected cases from inhibition of the PCR). (ajtmh.org)
  • This process amplifies DNA in samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler. (wikipedia.org)
  • reagents
  • One lab area is dedicated to preparation and handling of pre-PCR reagents and the setup of the PCR reaction, and another area to post-PCR processing, such as gel electrophoresis or PCR product purification. (wikipedia.org)
  • template
  • If the template contains a G, it adds a C to the new chain, and so on to the end of the DNA strand. (accessexcellence.org)
  • Detection of bifidobacteria can be achieved using DNA extracted from human faeces as template in PCR reactions. (scribd.com)
  • In order to try to assess the extent of inhibition that occurs in a reaction, a control can be performed by adding a known amount of a template to the investigated reaction mixture (based on the sample under analysis). (wikipedia.org)
  • PCR is routinely assessed against a negative control reaction that is set up identically to the experimental PCR, but without template DNA, and performed alongside the experimental PCR. (wikipedia.org)
  • enzymatic
  • The one-step approach is thought to minimize experimental variation by containing all of the enzymatic reactions in a single environment. (wikipedia.org)
  • Samples
  • As well as methods for the removal of inhibitors from samples before PCR, some DNA polymerases offer varying resistance to different inhibitors and increasing the concentration of the chosen DNA polymerase also confers some resistance to polymerase-targeted inhibitors. (wikipedia.org)
  • resistance
  • Other valuable properties of the chimeric polymerases TopoTaq and PfuC2 include enhanced thermostability, specificity and resistance to contaminants and inhibitors. (wikipedia.org)
  • stochastic
  • However, above this size, product yields often decrease, as with increasing length stochastic effects such as premature termination by the polymerase begin to affect the efficiency of the PCR. (wikipedia.org)
  • Stochastic approaches for modelling in vivo reactions. (wikipedia.org)
  • Applications
  • Lukyanov S.A., Lukyanov K.A., Gurskaya N.G., Bogdanova E.A., Buzdin A.A. (2007) Selective Suppression of Polymerase Chain Reaction and Its Most Popular Applications. (springer.com)
  • Our experienced industry analysts assess growth opportunities, market sizing, technologies, applications, supply chains and companies with the singular goal of helping you make informed business decisions, free of noise and hype. (bccresearch.com)
  • single
  • PCR carries out one reaction per single sample. (wikipedia.org)
  • dPCR also carries out a single reaction within a sample, however the sample is separated into a large number of partitions and the reaction is carried out in each partition individually. (wikipedia.org)
  • Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes, i.e., their base pair length, should be different enough to form distinct bands when visualized by gel electrophoresis. (wikipedia.org)