• permease
  • Its permease members exhibit broad specificity for a range of sugars, rather than being specific for just one or a few sugars. (ebi.ac.uk)
  • Phospho-HPr then transfers the phosphoryl group to a sugar-specific permease which consists of at least three structurally distinct domains (IIA, IIB, and IIC) which can either be fused together in a single polypeptide chain or exist as two or three interactive chains, formerly called enzymes II (EII) and III (EIII). (wikipedia.org)
  • Mannose XYZ permease is a member of the family, with this distinct method being used by bacteria for sugar uptake particularly exogenous hexoses in the case of Mannose XYZ in order to release the phosphate esters into the cell cytoplasm in preparation for metabolism primarily through the route of glycolysis. (wikipedia.org)
  • membrane
  • It is known as multicomponent system that always involves enzymes of the plasma membrane and those in the cytoplasm. (wikipedia.org)
  • Ronald Kaback became interested in membrane transport at a time when studies on biological membranes were in their infancy, and in the early phase of his career, he developed a cell-free membrane system to study active transport. (wikipedia.org)
  • The system consisted of osmotically sealed membrane vesicles of defined orientation (right-side-out) that catalyze active transport essentially as well as intact cells, but lack subsequent metabolism of the solutes accumulated. (wikipedia.org)
  • With the emergence of molecular biology, he and his colleagues pioneered Cysteine-Scanning Mutagenesis in combination with chemical modification, as well as a battery of site-directed biophysical/biochemical techniques to demonstrate almost incontrovertibly that LacY functions by a mechanism involving alternating access of sugar- and proton-binding sites to either side of the membrane. (wikipedia.org)
  • fructose
  • This derepression of the fructose-PTS gene cluster is mainly modulated by the negative effector F-1-P, but reduced sensitivity to the other effectors, F-1,6-P or G-6-P might cause differential transcriptional regulation of genes of the general part of the PTS (ptsI, ptsH) and associated genes encoding sugar-specific functions (ptsF, ptsG, ptsS). (uni-bielefeld.de)
  • residues
  • Without a crystal structure, Kaback and colleagues succeeded in using the approach to obtain essential information about helix packing, the organization of the sugar-binding site, and the residues involved in H+ translocation and coupling. (wikipedia.org)
  • Annotation
  • p>Information which has been generated by the UniProtKB automatic annotation system, without manual validation. (uniprot.org)