Several protease inhibitors, representing different classes of proteases (AEBSF, Pepstatin A, E-64, TPCK, PMSF and aprotinin), were tested for inhibitory effects on an RSV A2 infection of HEp-2 cells. (biomedcentral.com)
Inhibitor of aspartic proteases1
Pepstatin A is well known to be an inhibitor of aspartic proteases such as pepsin, cathepsins D and E. Except for its role as a protease inhibitor, however, the pharmacological action of pepstatin A upon cells remain unclear. (wikipedia.org)
Proteases5
Pepstatin is a potent inhibitor of aspartyl proteases. (wikipedia.org)
This inhibition seems to be independent of the activities of proteases such as cathepsin D, because the formation of osteoclasts was not suppressed with the concentration that inhibited the activity of cathepsin D. Cell signaling analysis indicated that the phosphorylation of ERK was inhibited in pepstatin A-treated cells, while the phosphorylation of IκB and Akt showed almost no change. (wikipedia.org)
Pepstatin A is a highly effective inhibitor of aspartyl proteases, Pepstatin A is unique in that it contains statine -- an unusual amino acid that is active against acid proteases, including pepsin. (agscientific.com)
Often used to study HIV proteases, Pepstatin is used to better understand the mechanisms of HIV-infected cells. (agscientific.com)
Treatment of XS52 DC with pepstatin A, an inhibitor of aspartic acid proteases, completely abrogated their capacity to present native PPD, but not trypsin-digested PPD fragments to Th1 and Th2 cell clones. (uni-frankfurt.de)
Affinity Chromatography2
Purification of Cathepsin D by Affinity Chromatography on Pepstatin Sepharose Column (str. (srce.hr)
The corresponding enzyme was purified from grain extracts by affinity chromatography on a pepstatin-Sepharose column. (lu.se)
Picomolar concentrations1
Originally discovered and isolated from a range of bacterial species, specifically Actinomyces , Pepstatin A is able to inhibit pepsin using picomolar concentrations. (agscientific.com)
Lysosomal1
Patients with CLN2 NCL are deficient in a pepstatin-insensitive lysosomal peptidase called tripeptidyl peptidase 1 (TTP1). (medscape.com)
Pepsin2
To observe the catalytic activity of pepsin (in cleaving peptide bonds in hemoglobin) in the presence and absence of pepstatin. (openwetware.org)
We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. (openwetware.org)
Completely1
The pH optimum of the affinity-purified enzyme was between 3.5 and 3.9 as measured by hemoglobin hydrolysis and the enzymatic activity was completely inhibited by pepstatin a specific inhibitor of aspartic proteinases (EC 3.4.23). (lu.se)
Protease2
Pepstatin A is well known to be an inhibitor of aspartic proteases such as pepsin, cathepsins D and E. Except for its role as a protease inhibitor, however, the pharmacological action of pepstatin A upon cells remain unclear. (wikipedia.org)
17. Aspartyl protease inhibitor pepstatin binds to the presenilins of Alzheimer's disease. (nih.gov)
Inhibitor2
Pepstatin is a potent inhibitor of aspartyl proteases. (wikipedia.org)
The aspartate proteinase inhibitor, pepstatin, did not inhibit enzyme activity. (waikato.ac.nz)
Lysosomal1
Patients with CLN2 NCL are deficient in a pepstatin-insensitive lysosomal peptidase called tripeptidyl peptidase 1 (TTP1). (medscape.com)
The hydrogen bonding interactions and the conformation adopted by pepstatin are very similar to those found in complexes of pepstatin with other aspartic proteinases. (nih.gov)
Proteinases2
Proteinase activity was inhibited by diazoacetyl- -norleucine methyl ester in a slow and non-specific manner atypical of pepstatin-sensitive aspartate proteinases. (waikato.ac.nz)
Wai 21a proteinase may be classified as member of the pepstatin-insensitive group of aspartate proteinases. (waikato.ac.nz)
Cathepsin2
The protonation state changes due to plasmepsin II, cathepsin D and endothiapepsin binding to pepstatin are predicted to within 0.4 proton units at pH 6.5 and 7.0, respectively. (nih.gov)
SiR700-lysosome is based on the pepstatin A (a cathepsin D binding peptide) which has been labeled with the proprietary far-red fluorophore called silicon rhodamine 700 (SiR700). (cytoskeleton.com)
Acid2
Pepstatin is practically insoluble in water, chloroform, ether, and benzene, however it can be dissolved in methanol, ethanol, and DMSO with acetic acid, to between 1 and 5 mg/ml. (wikipedia.org)
Characterisation of a thermostable pepstatin-insensitive acid proteinase from a Bacillus sp. (waikato.ac.nz)