The enzyme Uridine monophosphate synthase (EC 4.1.1.23, UMPS) (orotate phosphoribosyl transferase and orotidine-5'-decarboxylase) catalyses the formation of uridine monophosphate (UMP), an energy-carrying molecule in many important biosynthetic pathways. (wikipedia.org)
Many studies investigated the binding of a potent inhibitor of OMP decarboxylase, 6-hydroxy uridine monophosphate (BMP, a barbituric acid derivative), within the active site, to identify which essential amino acid residues are directly involved with stabilization of the transition state. (wikipedia.org)
After addition of ribose-P to orotate by OPRTase to form orotidine-5'-monophosphate (OMP), OMP is decarboxylated to form uridine monophosphate by ODCase. (wikipedia.org)
Orotate4
This bifunctional enzyme is named UMP synthase and it also catalyzes the preceding reaction in pyrimidine nucleotide biosynthesis, the transfer of ribose 5-phosphate from 5-phosphoribosyl-1-pyrophosphate to orotate to form OMP. (wikipedia.org)
Orotate PRTase activity is activated by low concentrations of OMP, phosphate, and ADP. (wikipedia.org)
We have previously shown that orotate phosphoribosyltransferase (OPRT) and orotidine 5'-monophosphate decarboxylase (OMPDC) in human malaria parasite Plasmodium falciparum form an enzyme complex, containing two subunits each of OPRT and OMPDC. (unboundmedicine.com)
Orotidine 5'-phosphate decarboxylase (OMP decarboxylase) or orotidylate decarboxylase is an enzyme involved in pyrimidine biosynthesis. (wikipedia.org)
In yeast and bacteria, OMP decarboxylase is a single-function enzyme. (wikipedia.org)
At low enzyme concentration and low OMP concentrations, OMP decarboxylase shows negative cooperativity, whereas, at higher OMP concentrations, the enzyme shows positive cooperativity. (wikipedia.org)
Kinetic parameters (K(m), k(cat) and k(cat)/K(m)) of the enzyme complex were 5- to 125-folds higher compared to the monofunctional enzyme. (unboundmedicine.com)
URA3 gives yeast the ability to synthesize (create) an enzyme called Orotidine 5'-phosphate decarboxylase (ODCase), so they can in turn create an essential nutrient for survival. (amino.bio)
The ability of the enzyme to use both O 2 and fumarate in cofactor reoxidation enables it to function under both aerobic and anaerobic conditions [5]. (enzyme-database.org)
The enzyme is a member of the succinate dehydrogenase/fumarate-reductase family of enzymes [5]. (enzyme-database.org)
ODCase2
Orotidine 5'-monophosphate decarboxylase (ODCase) has evolved to catalyze the decarboxylation of orotidine 5'-monophosphate without any covalent intermediates. (nih.gov)
6-Iodouridine 5'-monophosphate is a novel covalent inhibitor of ODCase, and its nucleoside analogue paves the way to a new class of inhibitors against malaria. (nih.gov)
Decarboxylation of orotidine1
Orotidine-5'-monophosphate decarboxylase (OMPD) catalyzes the decarboxylation of orotidine-5'-monophosphate (OMP) to uridine-5'-monophosphate (UMP) in an extremely proficient manner. (rcsb.org)
Carbamoyl-phosphat2
Carbamoyl-phosphate synthase large chain [Ensembl]. (ntu.edu.sg)
MGS-like domain, Carbamoyl-phosphate synthase L chain, Carbamoyl-phosphate synthetase large chain [Interproscan]. (ntu.edu.sg)
Yeast6
OMP decarboxylase has been a frequent target for scientific investigation because of its demonstrated extreme catalytic efficiency and its usefulness as a selection marker for yeast strain engineering. (wikipedia.org)
Mutations in the gene encoding OMP decarboxylase in yeast (URA3) leads to auxotrophy in uracil. (wikipedia.org)
In addition, a function OMP decarboxylase renders yeast strains sensitive to the molecule 5-fluoroorotic acid (5-FOA). (wikipedia.org)
The establishment of the URA3 gene as a selection marker with both positive and negative selection strategies has made the controlled expression of OMP decarboxylase a significant laboratory tool for the investigation of yeast genetics. (wikipedia.org)
A positive selection for mutants lacking orotidine-5̓-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. (microbiologyresearch.org)
OMP decarboxylase is known for being an extraordinarily efficient catalyst capable of accelerating the uncatalyzed reaction rate by a factor of 1017. (wikipedia.org)
To put this in perspective, the uncatalysed reaction which would take 78 million years to convert half the reactants into products is accelerated to 18 milliseconds when catalyzed by OMP decarboxylase. (wikipedia.org)
The exact mechanism by which OMP decarboxylase catalyzes its reaction has been a subject of rigorous scientific investigation. (wikipedia.org)
Catalysis of the reaction: H+ + orotidine 5'-phosphate = CO2 + UMP. (planteome.org)
An extreme example is orotidine 5'-phosphate decarboxylase , which allows a reaction that would otherwise take millions of years to occur in milliseconds. (ne.jp)
V, Patschkowski T, Niehaus K: Identification of the bacterial superoxide dismutase (SodM) as plant-inducible elicitor Orotidine 5′-phosphate decarboxylase of an oxidative burst reaction in tobacco cell suspension cultures. (liverxreceptor-signal.com)
Monophosphate1
We discovered that 6-iodouridine 5'-monophosphate (6-iodo-UMP) irreversibly inhibits the catalytic activities of ODCases from Methanobacterium thermoautotrophicum and Plasmodium falciparum. (nih.gov)
Ribose1
Disorders of Purine and Pyrimidine Metabolism Georges van den Berghe PURINE METABOLISM METABOLIC PATHWAYS Purines comprise bases, nucleosides in association with ribose or deoxyribose, and nucleotides with one or more added phosphate groups. (euroasfalti.net)
Mutants1
In our results, the sodium resistance exhibited by LpΔclpP mutant (Figure 5), which is a phenotype shared by the mutants without functional Dot/Icm T4SS [48, 64], together with the comparable decline in intracellular multiplication observed in LpΔclpP and ΔdotA mutants (Figure 7), suggest a role of ClpP in T4SS-dependent virulence through degrading a repressor or activating an up-regulator of the substrate(s) of ClpP. (peptide-solubility.com)
These separate conformational forms display different enzymatic activities, with the UMP synthase monomer displaying low decarboxylase activity, and only the 5.6 S dimer exhibiting full decarboxylase activity. (wikipedia.org)
OMP is an allosteric activator of OMP decarboxylase activity. (wikipedia.org)
Pyrimidine2
pyrimidine catabolism phosphate synthetase-II undergo breakdown such that useful parts can be triggered by administration of 5-fluorouracil to with! (euroasfalti.net)
As a phosphoribosyltransferase is … metabolism of purine & pyrimidine nucleotides participate in many organisms carbamoyl phosphate is by. (euroasfalti.net)
Interproscan1
Orotidine 5'-phosphate decarboxylase / HUMPS family [Interproscan]. (ntu.edu.sg)
Strains1
Krol E, Becker A: Global transcriptional analysis of the phosphate starvation response in Sinorhizobium meliloti strains 1021 and 2011. (liverxreceptor-signal.com)
Candida1
5% of all Candida species except for C. krusei , in which it is detected in up to 28% of isolates ( 3 ). (cdc.gov)
Enzymatic1
This extreme enzymatic efficiency is especially interesting because OMP decarboxylases uses no cofactor and contains no metal sites or prosthetic groups. (wikipedia.org)
Potent1
Among these antibiotics Ribostamycin was identified as a potent drug against the Protein Disulphide Isomerase with a significant binding energy of -7.35Kcal/mol with formation of 5 hydrogen bonds. (ijpsr.com)
UMPS1
In vitro, monomeric UMPS, with a sedimentation coefficient S20,w of 3.6 will become a dimer, S20,w = 5.1 after addition of anions such as phosphate. (wikipedia.org)
Bacteria1
This property demonstrates the capacity of the bacteria to survive intracellularly 5 . (ijpsr.com)
Catalytic activities1
However, in mammals, OMP decarboxylase is part of a single protein with two catalytic activities. (wikipedia.org)
Resistance1
exhibit replicative forms such as reduced stress tolerance (Figure 2 and 3), cell elongation (Figure LY3023414 clinical trial 4), enhanced sodium resistance (Figure 5), impaired cytotoxicity and growth on amoebae plates (Figure 6) and severely compromised intracellular multiplication in amoebae host (Figure 7). (peptide-solubility.com)
Site1
In-silico analysis was carried out using Pyrex software and the active constituents of Diplocyclos palmatus were docked to the binding site of the Orotidine 5'-phosphate decarboxylase (PDB ID:2CZD). (jclmm.com)