• There are eight Octamer proteins in humans (Oct1-11), which have been renamed according to the different classes of POU domain. (wikipedia.org)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Transcription factors are proteins that regulate the transcription of genes, or the production of mRNA from DNA. (biolegend.com)
  • POU proteins are eukaryotic transcription factors containing a bipartite DNA binding domain referred to as the POU domain. (embl.de)
  • The acronym POU (pronounced 'pow') is derived from the names of three mammalian transcription factors, the pituitary-specific Pit-1, the octamer-binding proteins Oct-1 and Oct-2, and the neural Unc-86 from Caenorhabditis elegans. (embl.de)
  • Homeobox proteins as sequence-specific transcription factors. (embl.de)
  • The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. (bdbiosciences.com)
  • Octamer-binding transcription factor-4 (Oct4), a transcription factor belongs to the POU transcription factor family Class V, is fundamental for maintaining self-renewal ability and pluripotency of stem cells. (biomedcentral.com)
  • The transcription factors Oct4, Sox2, and Klf4 were found to trigger endogenous expression of pluripotent genes [2] and were identified as PTFs due to their ability to access closed chromatin [3] . (cytoskeleton.com)
  • stump TDscs also showed increasing levels of sRY-box 2 (sox2), octamer-binding transcription factor 4 (oct4), tenomodulin (TnMD), and scleraxis (scx) protein and gene expression. (elsevierpure.com)
  • receptor (T?R1), TGF-?1, octamer-binding transcription factor 4 (OCT4) and aldehyde dehydrogenase 1 family member A1 (ALDH1A1) messenger RNA (mRNA) expression levels were analysed using quantitative real-Time reverse-Transcriptase polymerase chain reaction (RT-qPCR). (ui.ac.id)
  • The authors of the present study previously demonstrated that ectopically induced transcription factors, Octamer-binding transcription factor 4 (OCT-4), sex-determining region Y-box 2 (SOX2), and Kruppel-like factor 4 ( KLF-4) (collectively known as OSK factors) among mammals can reverse aging by restoring youthful patterns of deoxyribonucleic acid (DNA) methylation, transcriptomic profiles, and tissue functioning without cell identity loss. (news-medical.net)
  • To fit it into a nucleus with a diameter of ~10 μm, DNA is wrapped around histone octamers, creating strings of nucleosomes that can be further organized into higher-order levels of compaction [ 1 ]. (biomedcentral.com)
  • Binding of sequence-specific transcription factors and associated chromatin-modifying enzymes can induce post-translational modification of histone tails and can facilitate nucleosome removal [ 3 - 5 ], which can turn functional sequences such as promoters and enhancers into active, nucleosome-depleted sites [ 2 ]. (biomedcentral.com)
  • DNA in chromatin is organized in arrays of nucleosomes 1 .Two copies of each histone protein, H2A, H2B, H3 and H4, are assembled into an octamer that has 145?147 base pairs (bp) of DNA wrapped around it to form a nucleosome core (of relative molecular mass 206K). (nature.com)
  • The repeating nucleosome cores further assemble into higher-order structures which are stabilized by the linker histone H1 and these compact linear DNA overall by a factor of 30?40. (nature.com)
  • Below we discuss mechanisms in the form of pioneer transcription factors, histone modifications, and recently identified actin-dependent mechanisms that regulate chromosome accessibility and gene regulation during cellular reprogramming. (cytoskeleton.com)
  • PTFs also enable other transcription factors, histone modifiers, and nucleosome remodeling complexes to alter the chromatin state and promote gene expression of silenced genes. (cytoskeleton.com)
  • We show, in transient transfection assays, that the POU domain transcription factor, SCIP/Tst-1, specifically activates α3 in a neural context. (elsevierpure.com)
  • Oct-7, 8 and 9, also known as "brain factors", are predominantly expressed in the central nervous system during embryonic development. (wikipedia.org)
  • Pax6 is a transcription factor present during embryonic development. (biolegend.com)
  • Oct-4 is a homeodomain transcription factor of the POU family. (oct-4.com)
  • CTCDOs exhibited a hybrid epithelial-mesenchymal transition (EMT) state and an increased expression of stemness-associated markers including the two homeobox transcription factors Goosecoid and Pancreatic Duodenal Homeobox Gene-1 (PDX1), which were also detected in CTCs from CRC patients. (biomedcentral.com)
  • To exert stimulatory or repressive effects on transcription, accessible regulatory DNA elements must be in close spatial proximity to susceptible genes. (biomedcentral.com)
  • Enhancers promote transcription by providing a binding platform for transcription factors [ 12 ] that can act on (distal) target genes through three-dimensional chromatin looping [ 13 - 15 ]. (biomedcentral.com)
  • The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
  • Studies on rodent UGT genes have demonstrated that the transcription factors hepatocyte nuclear factor 1 (HNF1) and CAAT-enhancer binding protein are important positive regulators of UGT expression in the liver (Hansen et al. (aspetjournals.org)
  • Insertions of TEs influence the transcriptional regulation of some genes as well as the termination of transcription. (ecancer.org)
  • One of the potential effects of TEs is the generation of new gene sequences [ 3 , 6 , 7 ] such as the envelope (env) genes of retroviral origin found in several mammals. (ecancer.org)
  • STAT3 protein belongs to a group of intracellular transcription factors that mediate a variety of functions such as cellular differentiation, proliferation, and apoptosis. (biolegend.com)
  • 2 Basic fibroblast growth factor (bFGF) is essential for maintaining the stemness of hESCs by highly activating mitogen-activated protein kinase (MAPK) extracellular signal-regulated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling, which plays an important role in the stemness of hESCs. (ashpublications.org)
  • Glucosamine entering the cell is also converted to glucosamine-6-phosphate using GNK (GlcNAc kinase). (biomedcentral.com)
  • In many of these cases, a single exon is involved, and its transcription yields a variant mRNA [ 5 ]. (ecancer.org)
  • The boosters' effects on VC6TF (cocktail 1 or C1, valproic acid, CHIR-99021, E-616452, tranylcypromine, and forskolin) were evaluated, and inhibitors of chromatin remodeling factors were incorporated to investigate rejuvenation barriers and drivers. (news-medical.net)
  • Octamer-3/4, also known as POU5F1, is one of the Yamanaka factors, which are critical for the maintenance and self-renewal of embryonic stem cells. (wikipedia.org)
  • Oct-6 expression is confined to embryonic stem cells and the developing nervous system and skin, while Oct-11 is also involved in skin differentiation. (wikipedia.org)
  • Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. (bdbiosciences.com)
  • Okamoto K, Okazawa H, Okuda A, Sakai M, Muramatsu M, Hamada H. A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. (bdbiosciences.com)
  • As transplantation settings, single-cell suspensions of neural cells at day time 8, which were strongly positive for nestin (1.0 105 cells, = 6) and vehicle (phosphate-buffered saline (PBS), = 11), were injected through the burr opening and 2.0 mm ventral to the dura having a 5-l Hamilton syringe (Hamilton Organization, Reno, NV, USA) attached to a. (buyresearchchemicalss.net)
  • The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
  • RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. (biolegend.com)
  • Objective: To investigate the auto-induction of transforming growth factor-b1 (TGF-?1) in breast cancer stem cells (BCSCs) and its effect on cell viability and stemness. (ui.ac.id)
  • Cancer genetics, DNA damage response, apoptosis, cell cycle My current research is focused on two transcription factors that respond to DNA damage and play important roles in regulating tumor development. (mdanderson.org)
  • Binding Assay Laboratories manufactures the transcriptiojn factor binding assay reagents distributed by Genprice. (bioinfolab.org)
  • First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). (koreascience.kr)
  • Description: A competitive ELISA for quantitative measurement of Goat Octamer Binding Transcription Factor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (bioinfolab.org)
  • POU-domain transcription factors: pou-er-ful developmental regulators. (embl.de)
  • Moreover, it was found that these two are firmly correlated, which means acute renal failure can lead to chronic type and vice versa [ 6 ]. (biomedcentral.com)
  • Undifferentiated mouse ESC from the ES-E14TG2a cell line stained using the BD Stemflow Mouse Pluripotent Stem Cell Transcription Factor Analysis Kit. (bdbiosciences.com)
  • The entire BD Mouse Pluripotent Stem Cell Transcription Factor Analysis Kit must be stored in the dark at 2° to 8°C. Do not freeze. (bdbiosciences.com)
  • Conclusion We found that a failed repair stump had increased cellularity that preserved both mesenchymal and haematopoietic stem cell characteristics, with higher collagen synthesis, MMp, and growth factor gene expression. (elsevierpure.com)
  • In the next step, the enzyme glucosamine-phosphate N-acetyltransferase (GNPNAT) catalyzes Ac-CoA and glucosamine-6-phosphate to generate N-acetylglucosamine-6-phosphate (GlcNAc-6P) and CoA. (biomedcentral.com)
  • These results lead us to propose that α3 activation by SCIP/Tst-1 is achieved via protein-protein interactions between SCIP/Tst-1 and a specific complement of transcription factors that act directly on the promoter. (elsevierpure.com)
  • The researchers used VC6TF and C6NYSA as basal reprogramming cocktails and supplemented them with boosters [sodium butyrate, alpha-ketoglutarate (α-KG), and basic fibroblast growth factor (bFGF)] to increase iPSC efficiency. (news-medical.net)
  • The encoded protein contains two different binding sites that are known to bind DNA and function as a regulator of gene transcription. (biolegend.com)
  • However, it differs from other aggressive B-cell lymphomas in that it shows defective immunoglobulin production despite the expression of OCT-2, BOB.1, and PU.1 transcription factors and the lack of IgV(H) gene crippling mutations. (nih.gov)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. (therabio.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in samples from serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (therabio.org)
  • Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)
  • Description: A sandwich ELISA kit for detection of Sialic Acid Binding Ig Like Lectin 6 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (therabio.org)
  • This subset of transcription factors, known as pioneer transcription factors (PTFs), possess the ability to engage closed chromatin that is not accessible by other types of transcription factors, establish gene expression, and elicit cell reprogramming. (cytoskeleton.com)
  • The Transcriptiojn Factor Binding Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (bioinfolab.org)
  • The non-apoptotic vesicles have been classified based on their biogenesis into exosomes derived from membranes of the endosomal compartment and microvesicles that are formed from the budding of a portion of the cell plasma membrane ( 5 , 6 ). (spandidos-publications.com)
  • Cell growth is primarily supported by growth factor-driven glucose and glutamine intake, which form building blocks for biosynthesis. (biomedcentral.com)
  • Glucose enters the cell and undergoes two-step conversion to fructose-6P (fructose-6-phosphate), after which approximately 95% of it proceeds to glycolysis and 3-5% of it is converted to glucosamine-6P (glucosamine-6-phosphate) by the enzyme GFAT (glutamine:fructose-6-phosphate amidotransferase), utilizing glutamine that enters the cell. (biomedcentral.com)
  • GNA1/GNPNAT1 (glucosamine-6-phosphate N-acetyltransferase) then converts glucosamine-6P (which can also be made by glucosamine entering the cell) into GlcNAc-6P (N-acetylglucosamine-6-Phosphate), also utilizing acetyl-CoA that is made from fatty acid metabolism. (biomedcentral.com)
  • The factors that govern this specificity of UGT expression remain largely unknown. (aspetjournals.org)
  • In addition, mutations in the SCIP/Tst-1 coding region that prevent the factor from binding to DNA with high affinity do not obliterate α3 activation. (elsevierpure.com)
  • Oct3/4, octamer-binding transcription element 3/4. (buyresearchchemicalss.net)
  • More than half of the cases displayed BCL-6 gene mutations, which usually occurred along with functioning somatic IgV(H) gene mutations and Bcl-6 and/or MUM1/IRF4 expression. (nih.gov)
  • Here, we test the hypothesis that variant(s) responsible for reduced KIAA0319 expression resides on the risk haplotype close to the gene's transcription start site. (ox.ac.uk)
  • compared with normal tendon, stump tissue had significantly higher gene expression of collagens I and III, matrix metalloproteinases (MMps), vascular endothelial growth factor (VeGF), platelet-derived growth factor (pDGF), and insulin-like growth factor (IGF). (elsevierpure.com)
  • A primary virulence factor of this pathogen is the Diagnostics, Cincinnati, OH). (cdc.gov)
  • Pax-6 acts as a critical gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. (biolegend.com)
  • Our results reveal that pMeCP2 is downstream of BDNF, a critical factor in ketamine and scopolamine antidepressant action. (nature.com)
  • The aim of the present study was to identify network structures of remitters and non-remitters in patients with first-episode psychosis (FEP) at baseline and the 6-month follow-up. (cambridge.org)
  • Global network metrics did not differ between the remitters and non-remitters at baseline or 6 months. (cambridge.org)
  • Maximal drug uptake occurred at 2 h, whereas maximal skin color changes occurred 6 h after a single application. (researchgate.net)
  • Application of a single 2- or 6-h dose of the 0.05% cream at 1600 h produced more extensive and prolonged changes in skin color over 24 h than a 0900-h application in the same subject. (researchgate.net)