OCLNProteinsELISA KitMeasured by Western blot analysisTyrosine phosphorylationZonulaClaudinsPermeabilityPhosphorylationClaudinEndothelialTight junction assemblyIntegral membranPutativeVascularBarrierPeptideInteractsSignificantlyInteractionDecreaseMechanismCellularDifferentialGeneIntegrityExperimentsHumanLevelsRoleCellStudyFunctionEffectDevelopment
OCLN6
- The kit CSB-EL016263HU is used for cell lysates sample and for the quantitative determination of human occludin (OCLN). (cusabio.com)
- There exsit the quantitiy difference between different cell type Occludin(OCLN). (cusabio.com)
- Description: Quantitativesandwich ELISA kit for measuring Human Occludin (OCLN) in samples from serum, plasma, tissue homogenates, cell lysates. (signetlabs.com)
- Description: A sandwich quantitative ELISA assay kit for detection of Human Occludin (OCLN) in samples from tissue homogenates, cell lysates or other biological fluids. (signetlabs.com)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Occludin (OCLN) in tissue homogenates, cell lysates and other biological fluids. (signetlabs.com)
- Proximity ligation assay, immunoblotting, and phosphatase assay were performed to study the effect of DUSP3 on the TJ protein occludin (OCLN). (biomedcentral.com)
Proteins8
- In standard cells, loss or disruption of occludin and other tight junction proteins leads to initiation of apoptosis through extrinsic pathways. (wikipedia.org)
- To understand the association between netrin‑1 and the TJ‑associated proteins, the expression levels of proteins involved in maintaining the integrity of the BBB, including netrin‑1, claudin‑5, occludin and zonula occluden (ZO)‑1, were investigated in the present study using quantitative polymerase chain reaction, western blot analysis and immunofluorescence. (spandidos-publications.com)
- The results demonstrated that the levels of mRNA transcription and protein expression of the TJ‑associated proteins, claudin‑5, occludin and ZO‑1, were significantly reduced following TBI. (spandidos-publications.com)
- Purpose: The levels of tight junction proteins (TJs), especially occludin correlate with blood-brain barrier (BBB) disruption caused by central nervous system (CNS) inflammation during perioperative period. (researchsquare.com)
- Cerebral microvascular endothelial cells are linked together through the interactions among multiple tight junction proteins including claudins, occludin and ZO-1 [ 5 ]. (researchsquare.com)
- The decreased expression of tight junction proteins, especially occludin, correlates with BBB disruption in several situations such as cerebral ischemic injury [ 7 ]. (researchsquare.com)
- We assessed changes in the gut microbiota, intestinal permeability, gut peptides, intestinal epithelial tight-junction proteins ZO-1 and occludin (qPCR and immunohistochemistry), hepatic and systemic inflammation. (bmj.com)
- Induced EndoMT cells exhibited up-regulation of mesenchymal markers, including collagen type I and α-smooth muscle actin, and a reduction in endothelial cell and junctional proteins, including von Willebrand factor, CD31, occludin, and vascular endothelial-cadherin. (surrey.ac.uk)
ELISA Kit1
- Description: A sandwich ELISA kit for detection of Occludin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (signetlabs.com)
Measured by Western blot analysis1
- The expression and phosphorylation of Hif-1α, VEGF, VEGFR-2, ERK and occludin were measured by Western blot analysis. (researchsquare.com)
Tyrosine phosphorylation3
- We also found that Ang-1 inhibits thrombin induced tyrosine phosphorylation of Occludin and promote Occludin interaction with Zona Occludens-1 (ZO-1) to stabilize TJs. (johnshopkins.edu)
- Summary Collectively, our findings suggest that in brain endothelial cells blocking PTPN-2 mediated tyrosine phosphorylation of Occludin is a novel mechanism to maintain BBB function, and may offer a key therapeutic strategy for neuro-inflammatory disorders associated with BBB disruption. (johnshopkins.edu)
- These changes were accompanied by reduced occludin tyrosine phosphorylation (75.7+/-8%) and increased ZO-1 serine/threonine phosphorylation (51.7+/-9% and 82.7+/-25%, respectively), modifications that could be completely blocked with tyrosine phosphatase and protein kinase C inhibitors (dephostatin and rottlerin, respectively). (edgehill.ac.uk)
Zonula1
- Together with Claudins, and zonula occludens-1 (ZO-1), occludin has been considered a staple of tight junctions, and although it was shown to regulate the formation, maintenance, and function of tight junctions, its precise mechanism of action remained elusive and most of its actions were initially attributed to conformational changes following selective phosphorylation, and its redox-sensitive dimerization. (wikipedia.org)
Claudins1
- Tight junctions (TJs) function primarily as a barrier against paracellular transport between epithelial cells and are composed mainly of occludin (OLD) and claudins (CLDs). (elsevierpure.com)
Permeability4
- The extracellular loops are thought to be involved in the regulation of paracellualr permeability and the second extracellular has been shown to be involved in the localization of occludin at the tight junction. (wikipedia.org)
- Occludin, a 65-kDa tetraspan integral membrane protein, plays a role in the formation and regulation of the tight junction paracellular permeability barrier. (sysy.com)
- Hirase T, Staddon JM, Saitou M, et al: Occludin as a possible determinant of tight junction permeability in endothelial cells. (spandidos-publications.com)
- The correlation between a decrease in occludin levels and the perturbation of the tight junction permeability barrier provides evidence for a role of occludin in the formation of the tight junction seal. (rupress.org)
Phosphorylation1
- CONCLUSIONS: Overall, these findings indicate that cyclic strain modulates both the expression and phosphorylation state of occludin and ZO-1 in vascular endothelial cells, with putative consequences for endothelial tight junction assembly and barrier integrity. (edgehill.ac.uk)
Claudin3
- The present study also demonstrated that netrin‑1 significantly increased the downregulation of claudin‑5, occludin and ZO‑1 expression levels induced by TBI, which provided a basis for further investigation on the role of netrin‑1 in the integrity of TJs and proper functioning of the BBB. (spandidos-publications.com)
- We now report that TJ function, as well as claudin-1 and occludin expression, change in parallel during late epidermal development. (nih.gov)
- Specifically, TJ block the paracellular movement of Lanthanum (La(3+)) early in rat in vivo prenatal epidermal development, at gestational days 18-19, with concurrent upregulation of claudin-1 and occludin. (nih.gov)
Endothelial7
- However, mounting evidence demonstrated that occludin is not only present in epithelial/endothelial cells, but is also expressed in large quantities in cells that do not have tight junctions but have very active metabolism: pericytes, neurons and astrocytes, oligodendrocytes, dendritic cells, monocytes/macrophages lymphocytes, and myocardium. (wikipedia.org)
- Occludin is localized at tight junctions of both epithelial and endothelial cells. (sysy.com)
- In this study, we investigated the effects of TNF-α and propofol on occludin expression in human cerebral microvascular endothelial cell line, D3 clone (hCMEC/D3 cells), and explored the underlying mechanisms. (researchsquare.com)
- It has been reported that TNF-α could decrease the expression of occludin in endothelial cells [ 13 ]. (researchsquare.com)
- Interestingly, our study revealed that depletion of PTPN-2 by siRNAs abolishes Ang-1 ability to promote tyrosine dephosphorylation of Occludin, resulting Occludin disassociation from ZO-1 and endothelial hyperpermeability. (johnshopkins.edu)
- We, therefore, examined the effects of cyclic strain on the expression, modification, and function of 2 pivotal endothelial tight junction components, occludin and ZO-1. (edgehill.ac.uk)
- In addition, there was a significant strain-dependent increase in endothelial occludin/ZO-1 association (2.0+/-0.1-fold) in parallel with increased localization of both occludin and ZO-1 to the cell-cell border. (edgehill.ac.uk)
Tight junction assembly1
- Studies have shown that rather than being important in tight junction assembly, occludin is important in tight junction stability and barrier function. (wikipedia.org)
Integral membran1
- Occludin, the putative tight junction integral membrane protein, is an attractive candidate for a protein that forms the actual sealing element of the tight junction. (rupress.org)
Putative1
- To study the role of occludin in the formation of the tight junction seal, synthetic peptides (OCC1 and OCC2) corresponding to the two putative extracellular domains of occludin were assayed for their ability to alter tight junctions in Xenopus kidney epithelial cell line A6. (rupress.org)
Vascular1
- La fuga vascular causada por la apertura de las uniones estrechas en la barrera hematorretiniana y el aumento de la permeabilidad vascular es un importante mecanismo patog nico de edema retiniano. (medigraphic.com)
Barrier5
- The N-terminus of occludin experimentally has been linked to involvement in tight junction sealing/barrier properties. (wikipedia.org)
- Indeed, MDCK cells lacking occludin and its homolog tricellulin exhibit less complex tight junction strand network and impaired barrier function. (wikipedia.org)
- Occludin plays a critical role in maintaining the barrier properties of a tight junction. (wikipedia.org)
- Thus, mutation or absence of occludin increases epithelial leakiness which is an important barrier in preventing metastasis of cancer. (wikipedia.org)
- A study revealed that truncation of occludin decreases transendothelial electrical resistance, suggesting a key role of occludin in the barrier function of the tight junction [ 8 ]. (researchsquare.com)
Peptide2
- The OCC2 peptide decreased the amount of occludin present at the tight junction, as assessed by indirect immunofluorescence, as well as decreased total cellular content of occludin, as assessed by Western blot analysis. (rupress.org)
- Binding studies by surface plasmon resonance spectroscopy and peptide mapping combined with comparative homology modeling utilizing crystal structures led for the first time to a molecular model revealing the binding of both occludin and a-catenin to the same binding site in ZO-1. (fu-berlin.de)
Interacts2
- Haskins J, Gu L, Wittchen ES, Hibbard J and Stevenson BR: ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin. (spandidos-publications.com)
- Strong spatial evidence indicates that the occludin C-terminal coiled-coil domain dimerizes and interacts as a four-helix bundle with the identified structural motifs in ZO-1. (fu-berlin.de)
Significantly3
- Loss of occludin or abnormal expression of occludin has been shown to cause increased invasion, reduced adhesion and significantly reduced tight junction function in breast cancer tissues. (wikipedia.org)
- Furthermore, patients with metastatic disease displayed significantly lower levels of occludin suggesting that the loss of occludin and thereby loss of tight junction integrity is important in metastatic development of breast cancer. (wikipedia.org)
- Results: TNF-α (10 ng/ml, 4 h) significantly decreased the expression of occludin, which was attenuated by propofol (25 μM). (researchsquare.com)
Interaction1
- The exact sites, structures and molecular mechanisms of interaction between junction organizing zona occludence protein 1 (ZO-1) and the tight junction protein occludin or the adherens junction protein a-catenin are unknown. (fu-berlin.de)
Decrease2
- Conclusion: TNF-α could decrease the expression of occludin via Hif-1α/VEGF/VEGFR-2/ERK signaling pathway, which was attenuated by propofol. (researchsquare.com)
- Pulse-labeling and metabolic chase analysis suggested that this decrease in occludin level could be attributed to an increase in turnover of cellular occludin rather than a decrease in occludin synthesis. (rupress.org)
Mechanism1
- In conclusion, a common molecular mechanism of forming an intermolecular helical bundle between the hinge region/GUK domain of ZO-1 and a-catenin and occludin is identified as a general molecular principle organizing the association of ZO-1 at adherens and tight junctions. (fu-berlin.de)
Cellular1
- The presence of occludin decreased cellular invasiveness and motility, enhanced cellular sensitivity to apoptogenic factors and lowered tumorigenesis and metastasis of the cancer cells. (wikipedia.org)
Differential1
- Differential behavior of E-cadherin and occludin in their colocalization with ZO-1 during the institution of epithelial apartment polarity. (daubnet.com)
Gene1
- Recent work, using molecular modeling, supported by biochemical and live-cell experiments in human cells demonstrated that occludin is a NADH oxidase that influences critical aspects of cell metabolism like glucose uptake, ATP production and gene expression. (wikipedia.org)
Integrity1
- Repeated exposure to ODE or H2S or ODE+H2S induced oxidative stress and cytokine production, decreased tight junction protein occludin and cytoskeletal protein ezrin expression, disrupted epithelial integrity and resulted in increased Klebsiella pneumoniae invasion. (cdc.gov)
Experiments1
- The dimerisation of occludin was supported by biophysical experiments (size exclusion chromatography, light scattering), while surface plasmon resonance spectroscopy found an oligomerisation of ZO-1. (fu-berlin.de)
Human2
- Furthermore, manipulation of occludin content in human cells is capable of influencing the expression of glucose transporters, and the activation of transcription factors like NFkB, and histone deacetylases like sirtuins, which proved capable of diminishing HIV replication rates in infected human macrophages under laboratory conditions. (wikipedia.org)
- I would like to know if # CSB-EL016263HU is good to detect and measure human occludin in the cytosol. (cusabio.com)
Levels2
- Studies involving high levels of expression of occludin in cancer cells have shown that occludin mitigates several important cancer proliferation properties. (wikipedia.org)
- In response to strain, both occludin and ZO-1 protein expression increased by 2.3+/-0.1-fold and 2.0+/-0.3-fold, respectively, concomitant with a strain-dependent increase in occludin (but not ZO-1) mRNA levels. (edgehill.ac.uk)
Role1
- Occludin also plays an important role in the apoptosis. (wikipedia.org)
Cell1
- The C-terminus of occludin is important in receiving and transmitting cell survival signals. (wikipedia.org)
Study1
- In this study, we sought to explore whether propofol could attenuate the effect of TNF-α on occludin expression and identify the underlying mechanisms. (researchsquare.com)
Function2
- Occludin is an important protein in tight junction function. (wikipedia.org)
- The phenotypical response of these mice to the lack of occludin suggest that the function of occludin is more complex than thought and requires more work. (wikipedia.org)
Effect2
- In addition, the inhibitors of Hif-1α, VEGF, VEGFR-2, and ERK could reduce the effect of TNF-α on occludin expression. (researchsquare.com)
- The effect on occludin was specific because other tight junction components, ZO-1, ZO-2, cingulin, and the adherens junction protein E-cadherin, were unaltered by OCC2 treatment. (rupress.org)
Development1
- Occludin protein may contribute to the development of cervical cancer. (cusabio.com)