• enzymology
  • In enzymology, a 3'-nucleotidase (EC 3.1.3.6) is an enzyme that catalyzes the chemical reaction a 3'-ribonucleotide + H2O ⇌ {\displaystyle \rightleftharpoons } a ribonucleoside + phosphate Thus, the two substrates of this enzyme are 3'-ribonucleotide and H2O, whereas its two products are ribonucleoside and phosphate. (wikipedia.org)
  • ecto
  • Gutensohn W., Thiel E., Buschette S. (1984) Ecto-5′-Nucleotidase as a Leukemia Marker. (springer.com)
  • The test may also be referred to as 5'NT Levels Blood Test, CDF73 Levels Blood Test, and Ecto-5'-Nucleotidase Levels Blood Test. (wikipedia.org)
  • Ecto-nucleotidase activities of promastigotes from Leishmania (Viannia) braziliensis relates to parasite infectivity and disease clinical outcome. (biomedsearch.com)
  • Given the fact that the activity of ecto-nucleotidases correlates with parasitism and the development of infection, we evaluated the activity of these enzymes in promastigotes from 23 L. braziliensis isolates as a possible parasite-related factor that could influence the clinical outcome of the disease. (biomedsearch.com)
  • One isolate with high (PPS6m) and another with low (SSF) ecto-nucleotidase activity were chosen for further studies. (biomedsearch.com)
  • CONCLUSIONS/SIGNIFICANCE: Our data suggest that ecto-nucleotidases present on the promastigote forms of the parasite may interfere with the establishment of the immune response with consequent impaired ability to control parasite dissemination and this may be an important factor in determining the clinical outcome of leishmaniasis. (biomedsearch.com)
  • Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PrP C and the immunosuppressive cell surface enzyme ecto-5′-nucleotidase CD73. (aacrjournals.org)
  • This specific enzyme function has evolved in blood-feeding parasites from the ecto-ATPdase/CD39 (E-NTPDases)-, Cimex-type- and 5'-nucleotidase/CD73 enzyme families. (up.ac.za)
  • Molecular modelling of these two proteins showed a similar protein structure to a periplasmic ecto-5'-nucleotidase from E. coli. (up.ac.za)
  • Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. (wikipedia.org)
  • Regulation of ecto-5'-nucleotidase activity via Ca2+-dependent, annexin 2-mediated membrane rearrangement? (wikipedia.org)
  • PMID
  • In Escherichia coli, SurE also has activity as a nucleotidase and exopolyphosphatase, and may be involved in the stress response [ PMID: 17561111 ]. (ebi.ac.uk)
  • Human
  • The similarity in the coding region to that of the human 5ʹ-nucleotidase cDNA sequence [Oka, Matsumoto, Hosokawa and Inoue (1994) Biochem. (biochemj.org)
  • activity
  • It exhibited both 5ʹ-nucleotidase and phosphotransferase activity, as well as immunological and kinetic properties similar to those of the enzyme purified from calf thymus. (biochemj.org)
  • sequence
  • The bovine cDNA sequence included the sequences codifying for six peptides obtained from 5ʹ-nucleotidase/phosphotransferase purified from calf thymus. (biochemj.org)
  • Two full-length putative 5'-nucleotidase isoforms were identified that shared significant sequence identity and similarity to a 5'-nucleotidase from R. (B.) microplus and putative apyrases from I. scapularis and R. appendiculatus. (up.ac.za)
  • Subsequent, Edman N-terminal sequencing and MS/MS analysis with purified enzyme identified peptide sequence fragments that shared a high degree of sequence identity with both 5'-nucleotidase isoforms. (up.ac.za)
  • Protocols
  • Therefore, in this study 5'-nucleotidase homologous transcripts were identified from O. savignyi salivary gland DNA, using a 5'-nucleotidase specific degenerate primer and RACE protocols. (up.ac.za)
  • Function
  • Nucleotidases have an important function in digestion in that they break down consumed nucleic acids. (wikipedia.org)
  • Some nucleotidases function outside the cell, creating nucleosides that can be transported into the cell and used to regenerate nucleotides via salvage pathways. (wikipedia.org)
  • known
  • Since 1974, when pyrimidin 5' nucleotidase deficiency was isolated, is known that hemolysis is related to the accumulation of pyrimidin nucleotides within the erythrocytes that behave as metabolic inhibitors. (biomedsearch.com)
  • cellular
  • Nucleotidases are essential for cellular homeostasis, because they are partially responsible for maintaining a balanced ratio of nucleotides to nucleosides. (wikipedia.org)
  • another
  • In the present work, we report two cases of hemolytic anemia secondary to congenital deficiency of pyrimidin 5' nucleotidase and another case secondary to lead poisoning. (biomedsearch.com)
  • cells
  • 5′-Nucleotidase (5′-N) as an ectoenzyme has the double advantage of being testable by an ordinary enzyme assay on intact cells and at the same time being detectable by immunological methods as a surface structure. (springer.com)