NDPKProteinsProtein kinaseEnzymeMitochondrialNM23Adenylate kinaseTriphosphateNucleotideTriphosphatesDictyosteliumNTPsTetramericGeneBacterialCatalyticPhosphoglycerateRolesSubcellular localizationInhibitsSequenceRegulatesCellularLiverSuggestsNucleotidesIntracellularDifferentialCellCrucialImportantDomain
NDPK8
- NDPK activities maintain an equilibrium between the concentrations of different nucleoside triphosphates such as, for example, when guanosine triphosphate (GTP) produced in the citric acid (Krebs) cycle is converted to adenosine triphosphate (ATP). (wikipedia.org)
- NDPK are found in all cells, displaying not much specificity towards the types of nucleoside bases and are capable of accepting nucleotides and deoxyribonucleotides as substrates or donors. (wikipedia.org)
- NDPK are involved in the synthesis of nucleoside triphosphates (NTP), such as guanosine triphosphate (GTP), cytidine triphosphate (CTP) and uridine triphosphate (UTP), thymidine triphosphate (TTP). (wikipedia.org)
- NDPK has also been discovered to act as a protein histidine kinase, which involves a reversible histidine phosphorylation as a well-known regulatory signal. (wikipedia.org)
- These oligomeric proteins exhibit nucleoside diphosphate kinase (NDPK) activity that catalyzes nonsubstrate specific conversions of nucleoside diphosphates to nucleoside triphosphates. (embl.de)
- nucleoside diphosphate kinase (NDPK). (powerofpositivity.com)
- The Nme gene/protein family of nucleoside diphosphate kinases (NDPK) was originally named after its member Nm23-H1/Nme1, the first identified metastasis suppressor. (bvsalud.org)
- Biochemical studies showed that hNME1 is regulated by CoA through both covalent and non-covalent binding, which leads to a decrease in the hNME1 nucleoside diphosphate kinase (NDPK) activity. (bvsalud.org)
Proteins1
- Many of the expanding roles of nucleoside diphosphate kinase have been attributed to its ability to interact with other proteins. (dundee.ac.uk)
Protein kinase3
- However, consumption of ATP would definitely influence the cellular energy balance, which brings upon the regulation of AMP-activated protein kinase (AMPK). (wikipedia.org)
- One proposal is an interaction with the cellular energy sensor AMP-activated protein kinase, and here, we apply the simple eukaryotic organism, Dictyostelium discoideum as a test model. (dundee.ac.uk)
- It phosphorylates first to monophosphate form by a viral-encoded protein kinase homologue, and then to diphosphate and triphosphate forms by cellular kinases. (medscape.com)
Enzyme4
- Nucleoside diphosphate kinase (Ndk) is an important enzyme that generates nucleoside triphosphates (NTPs) or their deoxy derivatives by terminal phosphotransfer from an NTP such as ATP or GTP to any nucleoside diphosphate or its deoxy derivative. (embl.de)
- Inhibition of this enzyme by the diphosphate nucleoside causes a reduction in the concentrations of deoxynucleotides, including dCTP. (globalrph.com)
- A new subfamily of short bacterial adenylate kinases with the Mycobacterium tuberculosis enzyme as a model: a predictive and experimental study. (rhea-db.org)
- A sequence comparison with 52 different forms of adenylate kinases (AK) suggests that the enzyme from M. tuberculosis belongs to a new subfamily of 'short' bacterial AKs. (rhea-db.org)
Mitochondrial1
- 2023. J. Cell Biol.https://doi.org/10.1083/jcb.202301091) report that a nucleoside diphosphate kinase, NME3, facilitates mitochondrial tethering prior to fusion through its direct membrane-binding and hexamerization but not its kinase activity. (bvsalud.org)
NM232
- NM23-NDP kinase. (embl.de)
- Lastly, ethanol increased the expression of Kiss1 metastasis-suppressor and the metastasis suppressor Nm23/nucleoside diphosphate kinase. (iiarjournals.org)
Adenylate kinase3
- Expression of nucleoside diphosphate kinase, mitochondria-associated adenylate kinase, and several mitochondria-associated creatine kinase isozymes was highest in the outer retina, whereas expression of cytosolic adenylate kinase and brain creatine kinase was higher in the cones, horizontal cells, and amacrine cells indicating the diversity of ATP-buffering strategies among retinal neurons. (molvis.org)
- The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes. (rhea-db.org)
- Differences in expression profiles, substrate specificities, kinetic properties and subcellular localization among the AK (adenylate kinase) isoenzymes have been shown to be important for maintaining a proper adenine nucleotide composition for many different cell functions. (rhea-db.org)
Triphosphate4
- Nucleoside diphosphate kinase activity involves the transfer of the γ-phosphate of nucleoside triphosphate (NTP) to nucleoside diphosphate (NDP), where N1 and N2 can be ribo- or deoxyribonucleosides. (wikipedia.org)
- Gemcitabine is metabolized intracellularly by nucleoside kinases to the active diphosphate (dFdCDP) and triphosphate (dFdCTP) nucleosides. (globalrph.com)
- The cytotoxic effect of gemcitabine is attributed to a combination of two actions of the diphosphate and the triphosphate nucleosides, which leads to inhibition of DNA synthesis. (globalrph.com)
- The reduction in the intracellular concentration of dCTP (by the action of the diphosphate) enhances the incorporation of gemcitabine triphosphate into DNA (self-potentiation). (globalrph.com)
Nucleotide4
- Nucleoside-diphosphate kinases (NDPKs, also NDP kinase, (poly)nucleotide kinases and nucleoside diphosphokinases) are enzymes that catalyze the exchange of terminal phosphate between different nucleoside diphosphates (NDP) and triphosphates (NTP) in a reversible manner to produce nucleotide triphosphates. (wikipedia.org)
- Catalytic mechanism of nucleoside diphosphate kinase investigated using nucleotide analogues, viscosity effects, and X-ray crystallography. (expasy.org)
- Nucleoside diphosphate kinase (NDK), which is widely conserved in both prokaryotes and eukaryotes, maintains a balanced pool of nucleotide triphosphates and their deoxy derivatives. (openbiochemistryjournal.com)
- Synthesis of 8-hydroxy and 8-mercapto derivatives of acyclic adenine nucleoside and nucleotide analogs. (cas.cz)
Triphosphates4
- Maintenance of the level of nucleoside triphosphates (NTPs) as well as their corresponding deoxy derivatives (dNTPs) is crucial to all growth and developmental processes. (openbiochemistryjournal.com)
- These are enzymes that catalyze nonsubstrate specific conversions of nucleoside diphosphates to nucleoside triphosphates. (embl.de)
- Nucleoside diphosphate kinases ( EC 2.7.4.6 ) (NDK) are enzymes required for the synthesis of nucleoside triphosphates (NTP) other than ATP. (embl.de)
- First, gemcitabine diphosphate inhibits ribonucleotide reductase, which is responsible for catalyzing the reactions that generate the deoxynucleoside triphosphates for DNA synthesis. (globalrph.com)
Dictyostelium2
- The hexameric NDP kinase from Dictyostelium discoideum displays one single, irreversible differential scanning calorimetry peak (Tm 62 degrees C) over a broad protein concentration, indicating a single step denaturation. (protabank.org)
- The NDP kinase from Escherichia coli behaves like the P105G mutant of the Dictyostelium NDP kinase. (protabank.org)
NTPs1
- generally, kinases bring in NTPs for nucleic acid synthesis. (wikipedia.org)
Tetrameric1
- Thermal stability of hexameric and tetrameric nucleoside diphosphate kinases. (protabank.org)
Gene1
- Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. (novusbio.com)
Bacterial2
- Nucleoside diphosphate kinase: role in bacterial growth, virulence, cell signalling and polysaccharide synthesis. (embl.de)
- The eukaryotic nucleoside diphosphate (NDP) kinases are hexamers, while the bacterial NDP kinases are tetramers made of small, single domain subunits. (protabank.org)
Catalytic1
- Nucleoside diphosphate kinases (NDK) are characterized by high catalytic turnover rates and diverse substrate specificity. (rcsb.org)
Phosphoglycerate1
- Global mapping of protein-metabolite interactions in Saccharomyces cerevisiae reveals that Ser-Leu dipeptide regulates phosphoglycerate kinase activity. (mpg.de)
Roles1
- Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases. (mpg.de)
Subcellular localization1
- We found that cytoplasmic NDP kinase can be separated into two populations according to subcellular localization and response to extracellular stimuli. (liberty.edu)
Inhibits2
- Lenvatinib is an oral multi-kinase inhibitor that inhibits mainly vascular endothelial growth factor receptor 1-3, fibroblast growth factor receptor (FGFR) 1-4, platelet-derived growth factor receptor (PDGFR) α, c-KIT, and RET ( 11 ). (spandidos-publications.com)
- Transfection of cells with activated Rac mimics, whereas expression of dominant negative Rac inhibits, the effects of extracellular stimulation on the translocation of NDP kinase to the cell cortex. (liberty.edu)
Sequence1
- IC1 has a unique primary structure, the N-terminal part is homologous to the sequence of thioredoxin, the middle part consists of three repetitive sequences homologous to the sequence of nucleoside diphosphate kinase, and the C-terminal part contains a high proportion of negatively charged glutamic acid residues. (embl.de)
Regulates1
- and Otero, Angela de S., "Receptor Activation Regulates Cortical, but not Vesicular Localization of NDP Kinase" (2003). (liberty.edu)
Cellular2
- Cellular ATP is buffered by specialized equilibrium-driven high-energy phosphate (~P) transferring kinases. (molvis.org)
- Thus, the localization of the two NDP kinase pools identified here is regulated independently by distinct cellular components: the appearance of cortical NDP kinase is a consequence of Rac activation, whereas vesicular NDP kinase is responsive to microtubule dynamics and nucleotides, in particular GTP. (liberty.edu)
Liver1
- All non-nucleoside reverse transcriptase inhibitors (NNRTIs) are metabolized in the liver by CYP3A isoenzymes. (medscape.com)
Suggests1
- This suggests that MGCs utilize TCA cycle anaplerosis and cataplerosis to generate GTP and ~P transferring kinases to produce ATP that supports MGC energy requirements. (molvis.org)
Nucleotides1
- Binding of NDP kinase to this fraction is reduced by ATP and abolished by GTP, as well as guanine nucleotides that are NDP kinase substrates. (liberty.edu)
Intracellular2
- Another pool of NDP kinase accumulates independently of stimulation around intracellular vesicles. (liberty.edu)
- These results suggest that in fibroblasts NDP kinase participates in Racrelated cortical events and in GTP-dependent processes linked to intracellular vesicle trafficking. (liberty.edu)
Differential1
- The thermostability of NDP kinases of each class was studied by differential scanning calorimetry and biochemical methods. (protabank.org)
Cell2
- In cell-free assays NDP kinase binds tightly to membrane vesicles associated with taxol-stabilized microtubules. (liberty.edu)
- Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)
Crucial1
- Lenvatinib, a multi‑kinase inhibitor, serves a crucial role in the treatment of unresectable hepatocellular carcinoma (HCC). (spandidos-publications.com)
Important1
- The detailed study of their thermostability is important, since biological effects of thermolabile NDP kinases have been described in several organisms. (protabank.org)
Domain1
- They all possess nucleoside diphosphate kinase domain (NDK). (bvsalud.org)