• Modified nucleic acids such as peptide nucleic acid (PNA) offer advantages compared to conventional DNA probes allowing for faster hybridization, better stability and minimal sample preparation for direct detection of pathogens. (psu.edu)
  • Visualization of the mycelia of wood-rotting fungi by fluorescence in situ hybridization using a peptide nucleic acid probe. (bvsalud.org)
  • White rot fungus , Phanerochaete chrysosporium , and brown rot fungus , Postia placenta , grown on agar plates, were visualized by fluorescence in situ hybridization (FISH) using a peptide nucleic acid (PNA) probe. (bvsalud.org)
  • Peptide nucleic acid (PNA) amphiphiles: synthesis, self-assembly, and duplex stability. (umassmed.edu)
  • Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluid s and sputum. (cdc.gov)
  • Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. (wikipedia.org)
  • Data acquisition in in situ hybridization experiments is performed using a fluorescence microscope or with a cell imaging system, such as INCELL Analyzer. (qima-lifesciences.com)
  • A short introduction how cytogenetics and molecular cytogenetics were established is followed by technical aspects of fluorescence in situ hybridization (FISH). (frontiersin.org)
  • This review is about "molecular cytogenetics" including 1) the historical perspective of its development from cytogenetics, 2) technical aspects, 3) available probe sets, and 4) variants and applications of the basic fluorescence in situ hybridization (FISH) approach. (frontiersin.org)
  • 2016). Application of locked nucleic acid-based probes in fluorescence in situ hybridization . (up.pt)
  • 2015). Towards fluorescence in vivo hybridization (FIVH) detection of H. pylori in gastric mucosa using advanced LNA probes . (up.pt)
  • 2017). Detection and discrimination of biofilm populations using locked nucleic acid/2'-O-methyl-RNA fluorescence in situ hybridization (LNA/2'OMe-FISH) . (up.pt)
  • The intensity of fluorescence is measured to quantify the amount of hybridization that has occurred between the probes and the target molecules. (tutorialspoint.com)
  • Nucleic acid hybridization is at the core of many amplification-free molecular diagnostics and detection probe configuration is key to diagnostic performance. (psu.edu)
  • Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). (cdc.gov)
  • Detection of 13 foodborne pathogens in aquatic products using visual chromogenic chips based on asymmetric multiplex polymerase chain reaction and nucleic acid hybridization. (cabi.org)
  • The new guidelines further mention two specific antigen detection and nucleic acid probe-hybridization tests. (genomeweb.com)
  • After amplification the samples are typed by hybridization to the typing strips followed by colorimetric detection. (cdc.gov)
  • 3EO Health startup uses the Wyss Institute's user-friendly diagnostic nucleic acid detection technology together with additional components to build a digital platform that may empower patients with self-testing capabilities and access to results, information, and clinical partners. (harvard.edu)
  • Nucleic acid testing, which operates on this molecular level, has gradually become the mainstream of pathogen detection. (iso.org)
  • But it also covers the steps of the overall process of pre-examination, examination and post-examination for the analysis of nucleic acid amplification method for the detection of SARS-CoV-2, and specifies the methods and comprehensive assessment indicators including accuracy, limit of detection, inclusivity, and specificity, etc. (iso.org)
  • A person with laboratory isolation of typical gram-negative, oxidase-positive diplococcic by culture (presumptive Neisseria gonorrhoeae ) from a clinical specimen, or demonstration of N. gonorrhoeae in a clinical specimen by detection of antigen or detection of nucleic acid via nucleic acid amplification (e.g. (cdc.gov)
  • In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome). (wikipedia.org)
  • Since those original observations, many refinements have increased the versatility and sensitivity of the procedure to the extent that in situ hybridization is now considered an essential tool in cytogenetics. (wikipedia.org)
  • A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
  • Three-color in situ hybridization was applied to the study of numerical chromosome abnormalities as occur in human solid tumors. (nih.gov)
  • in situ hybridization is a technique for visualizing specific RNA or DNA within cell or tissue. (qima-lifesciences.com)
  • in situ hybridization can also be of interest for the analysis of specific gene expression profiles in sensitive samples, where total RNA extraction by RT-qPCR may not provide satisfactory results. (qima-lifesciences.com)
  • Bioalternatives can assist you in the implementation and in the analysis of your in situ hybridization experiments. (qima-lifesciences.com)
  • Do you have a project requiring in situ hybridization expertise? (qima-lifesciences.com)
  • 2015). Erratum to: Mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids (Applied Microbiology and Biotechnology, 10.1007/s00253-015-6389-4) . (up.pt)
  • Its principle is based on the use of a fluorescent probe which represents a nucleic acid sequence specific to a target-gene. (qima-lifesciences.com)
  • These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane. (thomassci.com)
  • In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. (thomassci.com)
  • Basically, technicians synthesize a single-stranded nucleic acid sequence complementary to the DNA or RNA of a specific pathogen as a probe. (iso.org)
  • If a probe can be complementarily paired with the nucleic acid of the pathogen to be tested, it is possible to observe the signals of the markers, so that the species of the pathogen to be tested can be confirmed. (iso.org)
  • Polymerase Chain Reaction [PCR]) or hybridization with a nucleic acid probe. (cdc.gov)
  • Beyond the bulk fluid assay and droplet devices, use of dsPNA probes may be advantageous in a wide variety of assays that employ homogenous nucleic acid hybridization. (psu.edu)
  • The hybridization occurs between the complementary sequences of the probes and the target molecules. (tutorialspoint.com)
  • My research focuses on the synthesis of modified nucleosides, oligonucleotides, metallated oligonucleotides, and their conjugation to develop hybridization probes. (utu.fi)
  • Modified nucleosides and nucleic acids, Oligonucleotide chemistry, developing covalently mercurated hybridization probes. (utu.fi)
  • In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. (wikipedia.org)
  • Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon. (aidstar-one.com)
  • Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. (bvsalud.org)
  • Hybridization is a basic property of nucleotide sequences and is taken advantage of in numerous molecular biology techniques. (wikipedia.org)
  • Secondary structure can be predicted from one or several nucleic acid sequences. (wikipedia.org)
  • The microarray technology is based on the hybridization of labelled DNA or RNA molecules to complementary sequences that are immobilized on a solid substrate. (tutorialspoint.com)
  • The principle of DNA microarray technology is based on the hybridization of complementary sequences of nucleic acid molecules. (tutorialspoint.com)
  • There are currently three methods for detecting SARS-CoV-2: nucleic acid tests, antigen tests and antibody tests. (iso.org)
  • many molecules have several possible three-dimensional structures, so predicting these structures remains out of reach unless obvious sequence and functional similarity to a known class of nucleic acid molecules, such as transfer RNA (tRNA) or microRNA (miRNA), is observed. (wikipedia.org)
  • Protein expression in living cells involves untethered intermediate molecules such as mRNA, enzymes, ribosomes, amino acids and polypeptides. (nature.com)
  • During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target molecules. (cdc.gov)
  • This temperature reduction allows hybridization to occur between the deoxyadenosine region on the capture oligomer and the poly-deoxythymidine molecules that are covalently attached to the magnetic particles. (cdc.gov)
  • Double-stranded nucleic acid molecules (DNA-DNA or DNA-RNA) which contain regions of nucleotide mismatches (non-complementary). (umassmed.edu)
  • In 1962 James Watson (b. 1928), Francis Crick (1916-2004), and Maurice Wilkins (1916-2004) jointly received the Nobel Prize in physiology or medicine for their 1953 determination of the structure of deoxyribonucleic acid (DNA). (wikipedia.org)
  • Deoxyribonucleic acid solution from calf thymus is suitable for use as a blocking agent in Southern hybridizations. (thomassci.com)
  • Fusing of two complementary strands of nucleic acids in vitro into double helices is called reassociation . (wikilectures.eu)
  • Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)
  • The principle of DNA microarray is based on the hybridization of DNA strands. (tutorialspoint.com)
  • Nucleic acid amplification tests (NAATs) are the recommended test method. (cdc.gov)
  • It notably includes recommendations for use of nucleic acid amplification tests, or NAATs, for Trichomonas vaginalis and routine trichomonas screening for high-risk populations. (genomeweb.com)
  • I later moved to the University at Albany, State University of New York (SUNY), USA for my postdoctoral research, where I worked on α-functionalization of Cysteine type amino acids with various alkyl halides using photocatalysis and dimeric compounds synthesis through dearomatization reactions. (utu.fi)
  • It can be accessed online at www.helmholtz-hiri.de/en/datasets/enterococcus through an instance of the genomic viewer JBrowse. (frontiersin.org)
  • It was used as negative control in DNA-DNA hybridization experiments using genomic DNA preparations of Vibrio parahaemolyticus and Vibrio alginolyticus. (thomassci.com)
  • First, ssDNA with a known sequence, complementary to the nucleic acid sought in the mixture, is attached to e.g. a membrane filter or a carbohydrate gel in the column. (wikilectures.eu)
  • During the flow of the investigated mixture of nucleic acids through a filter or column modified in this way, the sought complementary ssDNA or ssRNA associates with the DNA fixed to the carrier. (wikilectures.eu)
  • Such tests use a nucleic acid hybridization technique that is based on double-stranded complementary pairing of nucleic acids. (iso.org)
  • in vitro, they are formed by nucleic acid hybridization. (umassmed.edu)
  • Because this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. (aidstar-one.com)
  • Saline Sodium Citrate Buffer specifically developed for Northern and Southern transfer protocols and nucleic acid preparations. (aniara.com)
  • For experimental methods, see Nucleic acid structure determination . (wikipedia.org)
  • The nucleic acid test is the 'gold standard' for pathogenic diagnosis, faster and more accurate than the other methods. (iso.org)
  • ISO/TS 5798 mainly focuses on the use of nucleic acid amplification methods for diagnosis and screening of SARS-CoV-2 and proposes requirements and recommendations for the design, development, validation, verification and implementation of testing methods. (iso.org)
  • Where my research was focused on the biomimetic total Synthesis of complex natural products mainly bioactive lactones from carbohydrate scaffolds and Artemisinic acid glycoconjugates synthesis. (utu.fi)
  • Unfolding and folding of the double helix is ​​a common phenomenon in vivo (e.g. in the biosynthesis of nucleic acids). (wikilectures.eu)
  • They would then label it using, for example, radioisotopes or enzymes, and subject it to hybridization with the nucleic acids of the pathogen to be tested. (iso.org)
  • A common problem for researchers working with RNA is to determine the three-dimensional structure of the molecule given only a nucleic acid sequence. (wikipedia.org)
  • Overall, genetic relatedness of two species can be determined by hybridizing segments of their DNA (DNA-DNA hybridization). (wikipedia.org)
  • The filter is poured with a solution of radioactively labeled and short artificial nucleic acid that contains the desired sequence. (wikilectures.eu)
  • Automatic Nucleic Acid Hybridization Workstation-HONN LABORATORY INC. (robinhorsley.com)
  • After washing, the captured nucleic acid can be released (eluted) with a suitable hydrogen-bonding agent. (wikilectures.eu)
  • In the procedure, the DNA fragments are transferred from the gel electrophorogram to a material more suitable for hybridization, i.e. a nitrocellulose filter. (wikilectures.eu)
  • Suitable for use as a blocking agent in Southern hybridizations. (aidstar-one.com)
  • This test, using Hybrid Capture 2 technology, is a nucleic acid hybridization microplate assay with signal amplification. (cdc.gov)
  • Recently published, ISO/TS 5798 provides recommendations for the design, development, verification, validation and implementation of analytical tests for detecting the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) using nucleic acid amplification. (iso.org)
  • Most of us know that there are various types of COVID test available, but the new technical specification uses nucleic acid testing. (iso.org)
  • Epidemiology of transmissible diseases: Array hybridization and next generation sequencing as universal nucleic acid-mediated typing tools. (cdc.gov)
  • product and nucleic acid hybridization. (cdc.gov)
  • This graph shows the total number of publications written about "Nucleic Acid Heteroduplexes" by people in this website by year, and whether "Nucleic Acid Heteroduplexes" was a major or minor topic of these publications. (umassmed.edu)
  • Below are the most recent publications written about "Nucleic Acid Heteroduplexes" by people in Profiles. (umassmed.edu)