• tissues
  • Proliferation assays with mixed lymphocyte cultures or MLC's are also commonly used to evaluate the compatibility of donor and recipient tissues for organ transplants. (bio-medicine.org)
  • specific
  • An important development came when H.O. Smith, K.W. Wilcox, and T.J. Kelley, working at Johns Hopkins University in 1968, isolated and characterized the first restriction nuclease whose functioning depended on a specific DNA nucleotide sequence. (wikipedia.org)
  • Nucleases variously employ both nonspecific and specific associations in their modes of recognition and binding. (wikipedia.org)
  • A site-specific nuclease forms far stronger associations by contrast. (wikipedia.org)
  • SLX4, which is important in DNA repair by acting as a scaffold for structure-specific DNA repair nucleases, also binds to the TRFH domain of TERF2. (wikipedia.org)
  • known
  • Aspergillus nuclease S1 is known to be inhibited somewhat by 50 μM ATP and nearly completely by 1 mM ATP. (wikipedia.org)
  • A nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds between monomers of nucleic acids. (wikipedia.org)
  • Cell
  • Cell Proliferation Assays ( Abstract The cel. (bio-medicine.org)
  • Cell proliferation assays are employed frequently in immunological ca. (bio-medicine.org)
  • Results from a cell proliferation assay are presented which compare the performance of TopCount to the traditional counting method. (bio-medicine.org)
  • Cell proliferation assays are employed frequently in immunological, cancer and pharmaceutical research to assess the ability of both natural and synthetic compounds to stimulate or inhibit proliferation of lymphocytes. (bio-medicine.org)
  • Cell proliferation assays measure the incorporation of a radiolabeled DNA precursor, [ 3 H]-thymidine, into the replicating strands of DNA produced during cell division. (bio-medicine.org)
  • Cell proliferation assays have been performed with the MicroMate 196 Cell Harvester and TopCount, using Packard UniFilter plates. (bio-medicine.org)
  • Application of glutaraldehyde to in-cell Western assay for normalization. (biomedsearch.com)
  • By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA) into a cell, the cell's genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added. (wikipedia.org)
  • This can help control enzymes that may be damaging to a cell, like proteases or nucleases. (wikipedia.org)
  • primary
  • Nuclease primary structure is by and large poorly conserved and minimally conserved at active sites, the surfaces of which primarily comprise acidic and basic amino acid residues. (wikipedia.org)
  • numbers
  • The esterases to which nucleases belong are classified with the EC-numbers 3.1.11 - EC-number 3.1.31. (wikipedia.org)
  • For example, EcoRI comes from Escherichia coli RY13 bacteria, while HindII comes from Haemophilus influenzae strain Rd. Numbers following the nuclease names indicate the order in which the enzymes were isolated from single strains of bacteria: EcoRI, EcoRII. (wikipedia.org)