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  • tissues
  • Proliferation assays with mixed lymphocyte cultures or MLC's are also commonly used to evaluate the compatibility of donor and recipient tissues for organ transplants. (bio-medicine.org)
  • specific
  • An important development came when H.O. Smith, K.W. Wilcox, and T.J. Kelley, working at Johns Hopkins University in 1968, isolated and characterized the first restriction nuclease whose functioning depended on a specific DNA nucleotide sequence. (wikipedia.org)
  • Nucleases variously employ both nonspecific and specific associations in their modes of recognition and binding. (wikipedia.org)
  • A site-specific nuclease forms far stronger associations by contrast. (wikipedia.org)
  • SLX4, which is important in DNA repair by acting as a scaffold for structure-specific DNA repair nucleases, also binds to the TRFH domain of TERF2. (wikipedia.org)
  • made
  • The assay was made possibleby the discovery and characterization of bacteriophageencodedDNA-dependent RNA polymerases (SP6 T7and T3). (bio-medicine.org)
  • The assay was made possible by the discovery and characterization of bacteriophageencoded DNA-dependent RNA polymerases (SP6, T7, and T3). (bio-medicine.org)
  • example
  • For example, EcoRI comes from Escherichia coli RY13 bacteria, while HindII comes from Haemophilus influenzae strain Rd. Numbers following the nuclease names indicate the order in which the enzymes were isolated from single strains of bacteria: EcoRI, EcoRII. (wikipedia.org)
  • known
  • Aspergillus nuclease S1 is known to be inhibited somewhat by 50 μM ATP and nearly completely by 1 mM ATP. (wikipedia.org)
  • A nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds between monomers of nucleic acids. (wikipedia.org)
  • Cell
  • Cell Proliferation Assays ( Abstract The cel. (bio-medicine.org)
  • Cell proliferation assays are employed frequently in immunological ca. (bio-medicine.org)
  • Results from a cell proliferation assay are presented which compare the performance of TopCount to the traditional counting method. (bio-medicine.org)
  • Cell proliferation assays are employed frequently in immunological, cancer and pharmaceutical research to assess the ability of both natural and synthetic compounds to stimulate or inhibit proliferation of lymphocytes. (bio-medicine.org)
  • Cell proliferation assays measure the incorporation of a radiolabeled DNA precursor, [ 3 H]-thymidine, into the replicating strands of DNA produced during cell division. (bio-medicine.org)
  • Cell proliferation assays have been performed with the MicroMate 196 Cell Harvester and TopCount, using Packard UniFilter plates. (bio-medicine.org)
  • Application of glutaraldehyde to in-cell Western assay for normalization. (biomedsearch.com)
  • By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA) into a cell, the cell's genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added. (wikipedia.org)
  • This can help control enzymes that may be damaging to a cell, like proteases or nucleases. (wikipedia.org)
  • primary
  • Nuclease primary structure is by and large poorly conserved and minimally conserved at active sites, the surfaces of which primarily comprise acidic and basic amino acid residues. (wikipedia.org)
  • Polymerase
  • 2), was found to stimulate Page 125 AffinifyChromatographyofAuxin Receptor 125 Eschevichiacoli RNA polymerase-dependent incorpora- tion of ATP into 10 trichloroacetic acid (TCA) precip- itable material, no binding assays were conducted. (imbc-med.ru)
  • gene
  • Apart from the proper design of the nucleases to recognize and cut the selected site in the cell genome, two main goals need to be adequately addressed to optimize its function: the delivery of the tools into the desired cells and the selection of those where the gene editing process has occurred correctly. (intechopen.com)
  • Nuclease protection assays are used to map introns and 5' and 3' ends of transcribed gene regions. (wikipedia.org)
  • sequence
  • Hybridization assays involve labelled nucleic acid probes to identify related DNA or RNA molecules (i.e. with significantly high degree of sequence similarity) within a complex mixture of unlabelled nucleic acid molecules. (wikipedia.org)
  • addition
  • The limitations with the hybridization-ligation assay also apply to the dual ligation assay, with the 5'-end in addition to the 3'-end requiring to have a free hydroxyl (or a phosphate group). (wikipedia.org)
  • basic
  • Nuclease primary structure is by and large poorly conserved and minimally conserved at active sites, the surfaces of which primarily comprise acidic and basic amino acid residues. (wikipedia.org)
  • upon
  • Thus, optimal salt concentration in hybridization assays varies dependent upon the length and base composition of the analyte, capture and detection probes. (wikipedia.org)