• Proteins
  • Biomolecular NMR also provides valuable information about the interaction between RNA and diverse binding partners such as drugs, peptides, proteins or other nucleic acids. (els.net)
  • In 1990, Ad Bax and coworkers developed the triple resonance technology and experiments on proteins isotopically labelled with 15N and 13C, with the result that the spectra are dramatically simplified, greatly facilitating the process of resonance assignment, and increasing the size of the protein that may be determined by NMR. (wikipedia.org)
  • After returning to Switzerland, Wüthrich collaborated with, among others, Nobel laureate Richard R. Ernst on developing the first two-dimensional NMR experiments, and established the nuclear Overhauser effect as a convenient way of measuring distances within proteins. (wikipedia.org)
  • However, in large molecules such as proteins the number of resonances can typically be several thousand and a one-dimensional spectrum inevitably has incidental overlaps. (wikipedia.org)
  • SSNMR
  • Moreover, he develops methods to address challenging questions in structural biology where ssNMR can be applied with particular advantages, and have made particular contributions to the development of dynamic nuclear polarisation and proton detection using fast magic angle spinning. (wikipedia.org)
  • methods
  • Representative fits to Equation (2) (see 'Materials and Methods' section) and the η values for the 21 Ile resonances for the four states analyzed are shown in Figure 3.Figure 3. (nih.gov)
  • structure
  • A novel off-resonance rotating-frame 15N NMR spin relaxation experiment is used to characterize conformational fluctuations with correlation times between 32 and 175 microseconds in the third fibronectin type III domain of human tenascin-C. Conformational fluctuations of contiguous regions of the beta-sandwich structure of the type III domain may represent collective motions, such as transient twisting or breathing of the beta-sheets. (duke.edu)
  • 1H, 13C and 15N resonance assignments of YajG, an Escherichia coli protein of unknown structure and function. (ac.be)
  • Biomolecular structure is the intricate folded, three-dimensional shape that is formed by a molecule of protein, DNA, or RNA, and that is important to its function. (wikipedia.org)
  • He is a member of the Editorial Boards of the Journal of Biomolecular NMR and Structure. (wikipedia.org)