• The group uses biomolecular NMR spectroscopy coupled to biochemical, biophysical and computational methods to study the structure/function activity of proteins (or protein domains) involved in human diseases (e.g. cancer, genetic diseases). (hsr.it)
  • Triple resonance experiments are a set of multi-dimensional nuclear magnetic resonance spectroscopy (NMR) experiments that link three types of atomic nuclei, most typically consisting of 1H, 15N and 13C. (wikipedia.org)
  • These problems may be alleviated by using heteronuclear NMR spectroscopy which allows the proton spectrum to be edited with respect to the 15N and 13C chemical shifts, and also reduces the overlap of resonances by increasing the number of dimensions of the spectrum. (wikipedia.org)
  • He received a federal grant in 2017 from the National Institutes of Health to immerse undergraduates in hands-on use of the nuclear magnetic resonance (NMR) spectroscopy and researching trichomoniasis. (adelphi.edu)
  • We recovered 13C-labelled metabolites by microdialysis and utilised nuclear magnetic resonance spectroscopy (NMR) for identification and quantification.Of 33 patients with complete monitoring, 73% had MD at some point during monitoring. (cam.ac.uk)
  • Experimental methods of multi-dimensional NMR spectroscopy, dynamics and theoretical description of spin system evolution, multiple resonance NMR technique for structural studies of proteins and nucleic acids by NMR spectroscopy, contemporary methods of structural biology, studies of complex biomolecular systems. (muni.cz)
  • MOLMOL is a molecular graphics program designed for display and analysis of biological macromolecules, particularly protein or DNA structures determined by nuclear magnetic resonance spectroscopy. (biomedcentral.com)
  • Nuclear magnetic resonance (NMR) spectroscopy is the only method that provides high resolution structures in solution. (bacatec.de)
  • The scientific program stated early on Monday morning with a lecture series with the focus on the basic aspects of biomolecular NMR-spectroscopy and the structure determination of proteins, nucleic acids, and their complexes. (bacatec.de)
  • Nuclear Magnetic Resonance (NMR) spectroscopy has proven itself an extremely versatile tool for exploring a variety of biological problems. (unl.edu)
  • The Nuclear Magnetic Resonance Spectroscopy Facility provides a non-destructive analytical technique to many research groups in the department and outside. (uci.edu)
  • These solution NMR methods allow us to quantitatively investigate biomolecular electrostatics without any use of structure information. (utmb.edu)
  • NMR has been successfully used to determine the structure of numerous biomolecules, investigate biomolecular interactions, screen for potential drugs, determine structures of drug-complexes, monitor in vivo drug activity and analyze blood and urine for drug toxicity. (unl.edu)
  • In 1990, Ad Bax and coworkers developed the triple resonance technology and experiments on proteins isotopically labelled with 15N and 13C, with the result that the spectra are dramatically simplified, greatly facilitating the process of resonance assignment, and increasing the size of the protein that may be determined by NMR. (wikipedia.org)
  • Knowledge of the sequence of the Human Genome and thus, in principle, of basically the sequences of all proteins in humans, widens the demand for specialists able to determine structures of the individual constituent parts of biomolecular complexes of as well as the complexes in their entirety. (bacatec.de)
  • This article highlights the emerging MAS-DNP approaches and their applications to the analysis of biomolecular composites and intact cells to determine the folding pathway and ligand binding of proteins, the structural polymorphism of low-populated biopolymers, as well as the physical interactions between carbohydrates, proteins, and lignin. (portlandpress.com)
  • These experiments are often used to assign specific resonance signals to specific atoms in an isotopically-enriched protein. (wikipedia.org)
  • Many of these experiments have since become the standard set of experiments used for sequential assignment of NMR resonances in the determination of protein structure by NMR. (wikipedia.org)
  • These triple resonance experiments utilize the relatively large magnetic couplings between certain pairs of nuclei to establish their connectivity. (wikipedia.org)
  • Triple resonance experiments involving 31P may also be use for nucleic acid studies. (wikipedia.org)
  • Sequential assignment using triple resonance experiments These experiments are typically named by the nuclei (H, N, and C) involved in the experiment. (wikipedia.org)
  • Some of the experiments are used in tandem for the resonance assignment of protein, for example HNCACB may be used together with CBCA(CO)NH as a pair of experiments. (wikipedia.org)
  • Other experiments are also necessary to fully assign the side chain resonances. (wikipedia.org)
  • Triple resonance experiments can also be used in sequence-specific backbone resonance assignment of magic angle spinning NMR spectra in solid-state NMR. (wikipedia.org)
  • A large number triple-resonance NMR experiments have been created, and the experiments listed below is not meant to be exhaustive. (wikipedia.org)
  • The use of spherical rotors for magic angle spinning offers a number of advantages, including improved sample exchange, efficient microwave coupling for dynamic nuclear polarization nuclear magnetic resonance (NMR) experiments, and, most significantly, high frequency and stable spinning with minimal risk of rotor crash. (copernicus.org)
  • In order to make spherical rotors robust and accessible for magnetic resonance experiments and to design apparatuses capable of achieving very high MAS rates, we examined the spinning and stabilization mechanisms of these spherical rotors. (copernicus.org)
  • Recent oxygen-17 magnetic relaxation dispersion (MRD) experiments have shown that the vast majority of water molecules in the protein hydration layer suffer a mere twofold dynamic retardation compared with bulk water. (lu.se)
  • The course covers theoretical models for the structure and reactions of atomic nuclear, as well as experiments in nuclear physics and their scientific applications. (lu.se)
  • We are working on three areas of research: 1) Biomolecular Electrostatics , 2) Protein-DNA Dynamics , and 3) HMGB1 Biochemistry . (utmb.edu)
  • Nuclear Magnetic Resonance (NMR) is an important spectroscopic tool for the identification and structural characterization of molecules in chemistry and biochemistry. (bionmr.com)
  • Dynamic nuclear polarization allows hyperpolarization of most spins in small molecules encountered in chemistry and biochemistry. (bionmr.com)
  • A comprehensive analysis has identified and localized all yeast nucleoporins in nuclear pore complexes. (biomedcentral.com)
  • In the course of practice-oriented case studies, which were presented in the second half of the week, more detailed knowledge on structure determination of biomolecular complexes was taught. (bacatec.de)
  • Known particularly for its work in physical chemistry, the college also developed a reputation in nuclear chemistry, especially after the development of the cyclotron and the Radiation Laboratory under the leadership of physicist Ernest Lawrence. (berkeley.edu)
  • Organic & Biomolecular Chemistry, 2010. (bionmr.com)
  • In this 6-week course, students will learn thermodynamics of various biomolecular processes, including conformational changes, molecular association / dissociation, and protein-drug interactions. (utmb.edu)
  • Impact of nucleic acid self-alignment in a strong magnetic field on the interpretation of indirect spin-spin interactions. (muni.cz)
  • Obtaining highly resolved three dimensional structures of disease-related biomolecular assemblies is therefore of highest interest for pharmaceutical industry. (bacatec.de)
  • This Summer School will train students and post-doctoral researchers in the process of obtaining structural information of biomolecular assemblies suitable for solving biomedically relevant problems. (bacatec.de)
  • From new capabilities in nuclear magnetic resonance to the creation of dedicated spaces to conduct Indigenous research, Dalhousie is now set to empower its scholars to engage in some of the most sophisticated research in the world. (dal.ca)
  • Lisa Warner, an assistant research professor with Boise State's Biomolecular Research Center, is fascinated by the metabolic pathways found in living systems. (boisestate.edu)
  • It is anticipated that the capabilities of MAS-DNP in biomolecular and biomaterial research will be further enlarged by the rapid development of instrumentation and methodology. (portlandpress.com)
  • Solid-state nuclear magnetic resonance (ssNMR) is an indispensable tool for elucidating the structure and dynamics of insoluble and non-crystalline biomolecules. (portlandpress.com)
  • The NMR Center provides access to equipment for high resolution and solid state Nuclear Magnetic Resonance (NMR), as well as imaging, for studies on several topics, ranging from material science, diffusion, studies of small organic or inorganic molecules, to advanced studies of biomolecular structure and dynamics. (lu.se)
  • These tools also facilitate examination of theoretical models on biomolecular electrostatics. (utmb.edu)
  • The purpose of the course is to enhance the student's knowledge of theoretical and experimental nuclear physics. (lu.se)
  • Biomolecular electrostatics has been a subject of computational investigations based on 3D structures. (utmb.edu)
  • The signals observed in in vivo carbon-13 nuclear magnetic resonance (13C NMR) spectra are from 'mobile' molecules, i.e. those with relatively unrestricted motion. (westminster.ac.uk)
  • A way of increasing sensitivity of single scan NMR spectra by several orders of magnitude is through hyperpolarization of nuclear spins. (bionmr.com)
  • The recent advances in the sensitivity-enhancing technique magic-angle spinning dynamic nuclear polarization (MAS-DNP) have substantially expanded the territory of ssNMR investigations and enabled the detection of polymer interfaces in a cellular environment. (portlandpress.com)
  • All New users must attend a training course with the Biomolecular NMR Director before gaining access to the Core facility and its instrumentation. (unc.edu)
  • A separate 800 MHz NMR facility is available to all users for biomolecular NMR and other challenging problems. (uci.edu)
  • Using Nuclear Magnetic Resonance (NMR), a device that works similarly to the MRI machines found in hospitals, Warner and her team will be able to observe what is happening during the conversion process inside cells. (boisestate.edu)
  • This feature allows for the user-determination of the orientation of droplets relative to the main axes of the shim stack, permitting improved shimming and a more homogeneous magnetic field inside the droplet below the microcoil, which leads to improved spectral lineshape. (rsc.org)
  • Spectral density mapping at multiple magnetic fields suitable for C-13 NMR relaxation studies. (muni.cz)
  • The properties of water in biological systems have been are discussed, we emphasize magnetic-relaxation tech- studied for well over a century by a wide range of physical niques and, in particular, MRD. (lu.se)
  • This limitation is due to the need to assign NMR signals from the large number of nuclei in the protein - in larger protein, the greater number of nuclei results in overcrowding of resonances, and the increasing size of the protein also broadens the signals, making resonance assignment difficult. (wikipedia.org)
  • The main outcomes were fasting serum lipoprotein subclasses determined by nuclear magnetic resonance analysis. (nih.gov)
  • The magnetic field strength varies between 400 - 800 MHz 1H frequency. (lu.se)
  • Our nuclear magnetic resonance (NMR) methods are changing this situation. (utmb.edu)
  • Electrostatics is important for our fundamental understanding of biomolecular functions as well as for drug development. (utmb.edu)