OrganismsDiseasesChemicals and Drugs
Mycoplasma hyorhinisMycoplasmaMycoplasma InfectionsSerositisTenericutesMycoplasma fermentansJosamycinSwine DiseasesMycoplasma hominisAntigens, BacterialSwineMycoplasma genitaliumMycoplasma mycoidesBacterial ProteinsPneumonia of Swine, MycoplasmalDNA, BacterialMycoplasma hyopneumoniaeAntigens, SurfaceMycoplasma bovisLipoproteinsMycoplasma pulmonisMycoplasma gallisepticum
HyopneumoniaeHyosynoviaeSwineOraleCONTAMINATIONFermentansPigsBacteriaSpeciesPositive for mycoplasmaPathogenesisPorcineInfectionRespiratoryPneumoniaGallisepticumHominisClinical signsContaminantsDiagnosisColonizationInfectionsVeterinaryDetectRecombinantAssaysOrganismsLameness2020InvivoGenMollicutesArthritisStrainCommonlyFrequencyProteinCellMiceAbsenceMolecularAnalysisComponents
Hyopneumoniae17
Hyosynoviae3
  • Mycoplasma hyorhinis and Mycoplasma hyosynoviae in pig herds. (cabi.org)
  • Mycoplasma hyorhinis és M. hyosynoviae széles körben előforduló, fakultatív patogén kórokozók, melyek a sertések felső légútjait, illetve manduláit képesek kolonizálni. (huveta.hu)
  • Vaxxinova was on site with a seminar on autogenous vaccines in swine and a lecture on M. hyorhinis and M. hyosynoviae. (vaxxinova.de)
Swine5
  • The combined use of direct and indirect tests will help to better understand the dynamics of M. hyorhinis infections in swine populations. (nationalhogfarmer.com)
  • Although Mycoplasma hyorhinis is considered a commensal microorganism (i.e., detected by polymerase chain reaction in the absence of clinical disease) of the upper respiratory tract and tonsils of swine, it can cause disease resulting in lameness and loss of condition under specific conditions through mechanisms that are poorly understood. (nationalhogfarmer.com)
  • The ISU-VDL has recently developed a novel M. hyorhinis indirect enzyme-linked immunosorbent assay based on a recombinant chimeric made of variable lipoproteins specific (unique) to M. hyorhinis that allow for early and differential detection of M. hyorhinis infections with no cross-reactivity with other swine mycoplasmas. (nationalhogfarmer.com)
  • Mycoplasmas in swine. (cabi.org)
  • Mycoplasma hyorhinis is a facultative swine pathogen distributed worldwide. (huveta.hu)
Orale3
  • ATCC 29052) and M. orale (human throat commensal, cell culture isolate) are always included for mycoplasma media lot validation as these two species are common cell culture contaminants. (mycoplasma-exp.com)
  • We produced the mouse monoclonal antibodies (MAbs) and rabbit antibodies against Mycoplasma arginini, M. hyorhinis, M. orale and Acholeplasma laidlawii. (jejunu.ac.kr)
  • We constructed a sandwich ELISA using MH03 as capture, horse radish peroxidase labeled rabbit anti-A. laidlawii as detector, and could detect M.arginini with the detection limit of 0.5 ㎍/ml and M. hyorhinis, M. orale and A. laidlawii with the detection limit of 0.1 ㎍/ml. (jejunu.ac.kr)
CONTAMINATION9
  • With over 40 years of experience in developing mycoplasma solutions for the scientific community, InvivoGen's MycoStrip™ offers a new method for the detection of mycoplasma in cell culture, allowing you to combat the contamination. (invivogen.com)
  • MycoStrip™ is able to detect as low as 10-10 2 CFU/ml and thus, can be used to detect mycoplasma contamination before it significantly affects experimental results, which typically occurs at ~10 7 CFU/ml [1]. (invivogen.com)
  • Mycoplasma is a major problem in cell culture, with various sources of contamination such as lab operators, dirty water baths/incubators, and biological reagents. (invivogen.com)
  • Mycoplasma contamination of cells often goes unperceived. (invivogen.com)
  • The only way to confirm mycoplasma contamination is by routine testing using one or more special techniques, including direct growth on broth/agar, specific DNA staining, PCR, ELISA, RNA labeling and enzymatic procedures. (invivogen.com)
  • International Licensing Authorities stipulate cultural testing of vaccines and biologicals for the absence of mycoplasmas as, when properly applied, this is the most sensitive method for detecting mycoplasma contamination. (mycoplasma-exp.com)
  • Given the strong effects that mycoplasma cause within cell cultures, it is recommended that laboratories which routinely use cell culture should regularly test for mycoplasma contamination. (bio-rad-antibodies.com)
  • However, it is difficult to test for contamination because some strains of mycoplasma do not grow in vitro and require DNA staining and PCR detection methods. (bio-rad-antibodies.com)
  • What happens if my culture is positive for mycoplasma contamination? (eurofinsgenomics.eu)
Fermentans3
  • Mycoplasma fermentans has been associated with rheumatoid arthritis. (biomedcentral.com)
  • reported the presence of Mycoplasma fermentans in the synovial fluid of patients with rheumatoid arthritis [ 7 ], but these results could not be reproduced in further investigations probably because of the fastidious growth requirements of the microorganism [ 9 ]. (biomedcentral.com)
  • This mycoplasma has been detected in blood of patients with rheumatoid arthritis, producing systemic mycoplasmal infections [ 14 ], these results suggest that M. fermentans may play a role in the development of the disease. (biomedcentral.com)
Pigs5
  • Recently, M. hyorhinis -associated disease has emerged as an important contributor to mortality in nursery pigs [1]. (nationalhogfarmer.com)
  • Mycoplasma suis infections in pigs. (cabi.org)
  • Fortunately, Vaxxinova scientists have been studying pathogens like mycoplasmas and streptococci for more than 25 years and have found the specific isolates that have the most damaging impacts to pigs. (us.com)
  • Mycoplasma suis ( M. suis ) is an haemotropic Mycoplasma that adheres and invades erythrocytes and is responsible for infectious anaemia of pigs. (biomedcentral.com)
  • Mycoplasma suis (M. suis) is an uncultivable haemotropic Mycoplasma that targets red blood cells of pigs [ 3 ] and is responsible for infectious anaemia of pigs (IAP), historically known as porcine eperythrozoonosis [ 4 ]. (biomedcentral.com)
Bacteria8
  • Mycoplasma hyorhinis is a species of bacteria in the Mycoplasmataceae family. (wikipedia.org)
  • Rarely, it may cause mycoplasma arthritis, mycoplasmal polyserositis or mycoplasma septicaemia in piglets without the involvement of other bacteria. (wikipedia.org)
  • Mycoplasma and Acholeplasma are genera that comprise more than 100 recognized species in the class Mollicutes, which are bacteria that lack a cell wall. (invivogen.com)
  • Unlike bacteria or fungi, mycoplasmas do not cause consistently observable alterations in cell culture such as rapid pH change or culture turbidity [2,4] . (invivogen.com)
  • TLRs activate viral responses by recognizing pathogenic microorganisms such as viruses, bacteria, and mycoplasmas to release inflammatory cytokines [ 10 ]. (hindawi.com)
  • Mycoplasma are the smallest free living organisms, and are considered to be the simplest parasitic bacteria. (bio-rad-antibodies.com)
  • A species of gram-negative bacteria causing PNEUMONIA, MYCOPLASMA in calves. (uchicago.edu)
  • Mycoplasma dispar also possibly plays a synergistic role with other BACTERIA and VIRUSES to cause other RESPIRATORY TRACT DISEASES in calves. (uchicago.edu)
Species10
  • Overview of the general characteristics and classification of porcine Mycoplasma species. (cabi.org)
  • Simply prepare your sample and add our proprietary Reaction Mix to target and amplify the 16S rRNA gene for the most commonly found mycoplasma species in cell culture. (invivogen.com)
  • MycoStrip™ has been specifically designed to detect the Mycoplasma and Acholeplasma species that most commonly contaminate cell culture. (invivogen.com)
  • The DNA extracted from the samples were subjected to Mycoplasma genus-specific, followed by species-specific PCR. (jvas.in)
  • but further molecular detection performed through species-specific primers, revealed that 12 samples were positive for M. hyorhinis. (jvas.in)
  • Liquid and solid mycoplasma media formulations and their constituent batches are screened for their ability to support the growth of at least seven mycoplasma species before acceptance for use in Regulatory Tests. (mycoplasma-exp.com)
  • Among the established MAbs and rabbit anti-mycoplasmas, MAb MH03 against M. hyorhinis, and rabbit anti-A. laidlawii showed high cross reactivities to the four Mycoplasma species. (jejunu.ac.kr)
  • Rabbit antibodies against Mycoplasma species. (jejunu.ac.kr)
  • What mycoplasma species are detected with Mycoplasmacheck? (eurofinsgenomics.eu)
  • Mycoplasmacheck can detect all mycoplasma species that contaminate cell cultures. (eurofinsgenomics.eu)
Positive for mycoplasma1
Pathogenesis1
  • There is some evidence implicating M. hyorhinis in the pathogenesis of scleroderma, a chronic systemic disease of humans. (wikipedia.org)
Porcine3
  • M. hyorhinis is thought to facilitate and exacerbate the development of diseases such as porcine enzootic pneumonia and porcine reproductive and respiratory syndrome (PRRS). (wikipedia.org)
  • Immune responses against porcine Mycoplasma infections. (cabi.org)
  • and cell culture reagents such as bovine sera (M. arginine and A. laidlawii) or porcine trypsin (M. hyorhinis). (invivogen.com)
Infection2
  • 1. Clavijo MJ, Murray D, Oliveira S, Rovira A. Infection dynamics of Mycoplasma hyorhinis in three commercial pig populations. (nationalhogfarmer.com)
  • Nevertheless, mycoplasmas can have disastrous effects on eukaryotic cells, as they can alter every cellular function (proliferation, protein synthesis, susceptibility to viral infection, etc. (invivogen.com)
Respiratory3
  • For the prevention and treatment of bovine pneumonia associated with bovine respiratory Mycoplasma or Pasteurella multocida sensitive to tylosin. (farmacy.co.uk)
  • The primary habitats of human and animal mycoplasmas are the mucous surface of the respiratory and urogenital tracts, the eyes, mammary glands and joints [ 5 ]. (biomedcentral.com)
  • Mycoplasmas are common inhabitants of the human respiratory and genitourinary tracts where they produce chronic infections, they also are able to invade other tissues and in some cases induce an autoimmune response [ 5 ]. (biomedcentral.com)
Pneumonia1
  • Calves: Treatment and prevention of pneumonia caused by Mycoplasma spp when the disease has been established in the herd. (farmacy.co.uk)
Gallisepticum1
Hominis1
  • Bartholomew isolated M. hominis , M. hyorhinis and M. arthritidis from the synovial fluid in 14 of 17 patients with rheumatoid arthritis, systemic lupus erythematosus and Reiter's Syndrome in 1965 [ 8 ]. (biomedcentral.com)
Clinical signs1
  • A diagnosis of M. hyorhinis -associated disease is made based on history, clinical signs, typical gross and microscopic lesions, and isolation of M. hyorhinis by culture in specific media (i.e. (nationalhogfarmer.com)
Contaminants2
  • The aim of this study was to develop sandwich ELISA method for the detection of common mycoplasma contaminants in cell culture. (jejunu.ac.kr)
  • These results indicate that the sandwich ELISA developed in this study could be applied for the detection of the four major Mycoplasma contaminants in cell culture. (jejunu.ac.kr)
Diagnosis2
  • Due to the increased frequency of diagnosis and poor response to treatment in a subset of animals, there is an immediate need for antemortem diagnostic tools that are able to inform intervention strategies intended to prevent M. hyorhinis -associated disease. (nationalhogfarmer.com)
  • Mycoplasma hyorhinis -Field experiences in diagnosis and control. (nationalhogfarmer.com)
Colonization1
  • M. hyorhinis colonization is not uncommon and has been detected in nearly every production system, with variable between and within herd prevalence that varies by age and among production systems [2]. (nationalhogfarmer.com)
Infections2
  • Dr. Adrian Lührs presented the possibilities and limitations of using autogenous vaccines for mycoplasma and streptococcal infections. (vaxxinova.de)
  • You can detect mycoplasma infections though testing cultures on standardized agar, and identifying the typical "fried egg" morphology after 3-4 weeks. (bio-rad-antibodies.com)
Veterinary1
  • The Iowa State University Veterinary Diagnostic Laboratory accounted for an average of 26% of Mycoplasma -associated arthritis and lameness cases between 2013 and 2014, with an increasing frequency over the last several years. (nationalhogfarmer.com)
Detect2
Recombinant2
  • However, identification and functional analysis of such proteins is hampered by the lack of mutational systems in mycoplasmas and by a perceived limitation in translating recombinant mycoplasma genes containing UGA (Trp) codons in other eubacteria. (huji.ac.il)
  • It was initially detected by screening a recombinant phage genomic library with antibody from a host with M. hyorhinis-induced arthritis and was localized by Tn5 and deletion mutations affecting expression of antigenic translational products. (huji.ac.il)
Assays1
  • Easy to perform, and unlike most other mycoplasma detection assays, no special lab equipment is required. (invivogen.com)
Organisms2
  • Mycoplasmas are the smallest and simplest self-replicating organisms. (invivogen.com)
  • Mycoplasma Experience uses media which do support the growth of these fastidious organisms allowing us to offer a more sensitive cultural test than one using conventional media. (mycoplasma-exp.com)
Lameness1
  • The progressions of M. hyorhinis clinical disease (lameness and loss of condition) were evaluated in relation to the kinetics of the isotype-specific (IgG, IgA) antibody response in serum and bacterial shedding (PCR) in oral fluids using a cesarean-derived, colostrum-deprived pig model. (nationalhogfarmer.com)
20201
InvivoGen1
  • Since research organizations have limited personnel and must deal with compliance issues, InvivoGen has developed a simple and practical mycoplasma detection kit, PlasmoTest™, which sits right in the incubator alongside cells. (invivogen.com)
Mollicutes1
  • Proteins translocated across the single plasma membrane of mycoplasmas (class Mollicutes) represent important components likely to affect several interactions of these wall-less microbes with their respective hosts. (huji.ac.il)
Arthritis1
  • These abilities are used by mycoplasmas during the induction of natural arthritis in animals. (biomedcentral.com)
Strain1
  • TLR2 is highly conserved from Drosophila to humans and, to date, there have not been any reports of a mycoplasma strain able to go undetected by TLR2. (invivogen.com)
Commonly2
  • Friis) from affected tissues (e.g., fibrin from serous membranes, synovial tissue or joint fluid) or, more commonly, detection of M. hyorhinis by PCR in these same sample types. (nationalhogfarmer.com)
  • These findings may help explain why mycoplasmas are commonly isolated from the joints of rheumatic patients. (biomedcentral.com)
Frequency1
Protein1
  • Here we directly analyze a gene encoding a Mycoplasma hyorhinis protein capable of promoting its membrane translocation. (huji.ac.il)
Cell5
  • M. hyorhinis can prevent cell death caused by nicotinamide phosphoribosyltransferase inhibitors. (wikipedia.org)
  • Fusion proteins containing mature P101 sequences expressed mycoplasma epitopes and were found by cell fractionation and detergent phase partitioning to be integral membrane proteins in E. coli, suggesting a lack of signal peptide cleavage in this system. (huji.ac.il)
  • Detection of cell culture contaminating mycoplasma by MycoStrip™ is based on isothermal PCR. (invivogen.com)
  • However, conventional mycoplasma media do not support the growth of M. hyorhini s cultivar α , a cell culture contaminant. (mycoplasma-exp.com)
  • What is mycoplasma, and how does it affect your cell culture? (bio-rad-antibodies.com)
Mice1
  • Another study found that injecting M. hyorhinis into mice induces a scleroderma-like autoimmune disease. (wikipedia.org)
Absence1
  • The Mycoplasmacheck data report indicates the presence or absence of mycoplasma, as well as the presence or absence of PCR inhibitors. (eurofinsgenomics.eu)
Molecular1
Analysis1
  • Importantly, identification of P101 by direct analysis of its export function relied neither on prior identification of the mycoplasmal product nor on complete expression of the product from the cloned mycoplasma gene. (huji.ac.il)
Components1
  • PlasmoTest™ is based on detection of mycoplasmas by engineered cells that express Toll-like receptor 2 (TLR2) , a pathogen recognition receptor that detects bacterial membranes components. (invivogen.com)