TuberculosisNontuberculous MycobacteriaOrganismMycolicibacteriumSpeciesGenomeMycobacterialStrainBiofilm formationPorinBiosynthesisStrainsFortuitumProteinPathogenicGeneAviumMutantsPathway2023PutativeInfectionMacrophagesGenBankSusceptibilityLipoarabinomannanEnzymeCommonlySurvivalComplexIdentificationLaboratoryCell wallLaboratoriesCitationUniqueFoundMolecularRole
Tuberculosis29
- citation needed] Mycobacterium smegmatis shares the same peculiar cell wall structure of M. tuberculosis and other mycobacterial species. (wikipedia.org)
- There are many different kinds of specific secretion systems, and M. tuberculosis has an Snm (secretion in mycobacteria) protein secretion system. (wikipedia.org)
- Although the Snm secretion system is a key in determining M. tuberculosis virulence, all mycobacteria have genes encoding the components of this system. (wikipedia.org)
- M. smegmatis is commonly used to study Snm secretion because of its genetic similarities and analogous function to M. tuberculosis, as well as ease of growing in the lab. (wikipedia.org)
- Sulfolipid-1 (SL-1) is an abundant sulfated glycolipid and potential virulence factor found in Mycobacterium tuberculosis. (rcsb.org)
- Disruption of stf0 in Mycobacterium smegmatis and M. tuberculosis resulted in the loss of T2S and SL-1 formation, respectively. (rcsb.org)
- It contributes to the virulence of Mycobacterium tuberculosis. (jic.ac.uk)
- Mycobacterium tuberculosis is a facultative pathogen that causes tuberculosis and it accounted for 1.4 million deaths worldwide in 2011. (wits.ac.za)
- Mycobacterium tuberculosis (Mtb) is an intracellular human pathogen that has evolved to survive in a nutrient limited environment within the host for decades. (birmingham.ac.uk)
- The gene encoding methionine adenosyltransferase has been cloned from Mycobacterium tuberculosis and the model organism M. smegmatis . (biomedcentral.com)
- While the M. smegmatis enzyme could be functionally expressed, the M. tuberculosis homologue was insoluble and inactive under a large variety of expression conditions. (biomedcentral.com)
- Mycobacterium tuberculosis is difficult to kill for a number of reasons. (biomedcentral.com)
- In the examination of biochemical pathways in Mycobacterium tuberculosis , it would be ideal to identify processes where an enzyme plays a role in both active and chronic phase survival. (biomedcentral.com)
- the complete genome sequence of Mycobacterium tuberculosis continues to provide an invaluable resource to understand tuberculosis (TB), the leading cause of global infectious disease mortality. (jci.org)
- Whole-genome sequencing (WGS) of M. tuberculosis and related mycobacteria is now routine, allowing comparisons across time and space. (jci.org)
- Cholesterol is a crucial component in Mycobacterium tuberculosis virulence as it is required for phagocytosis of mycobacteria by macrophages . (bvsalud.org)
- Mycobacterium smegmatis is closely related to the pathogenic M. tuberculosis . (lynchburg.edu)
- The precise mechanism by which M. tuberculosis is able to evade acidification by the phagosomes of macrophages is not yet known, so understanding the interactions between macrophages and the cell wall components on Mycobacteria is of great relevance. (lynchburg.edu)
- The Mycobacterium tuberculosis capsule: a cell structure with key implications in pathogenesis. (nih.gov)
- This method has allowed the distinction of Mycobacterium tuberculosis from other mycobacteria and enabled the performance of antimicrobial susceptibility testing of mycobacteria. (medscape.com)
- We further show that bergenin induces Th1 immune responses and potently inhibits bacillary growth in a murine model of Mycobacterium tuberculosis infection. (frontiersin.org)
- O desenvolvimento de novas drogas, vacinas e técnicas de diagnóstico depende de uma melhor compreensão dos mecanismos de virulência de Mycobacterium tuberculosis. (scielo.br)
- This peptide has specific modes of action on Mycobacterium smegmatis, which is used as a model organism for Mycobacterium tuberculosis. (calstatela.edu)
- However, in the case of Mycobacterium tuberculosis ( Mtb ), the causative agent of tuberculosis (TB), Mtb -mediated drug metabolism plays a significant role in treatment outcomes. (nature.com)
- Mycobacterium tuberculosis Rv0927c Inhibits NF-κB Pathway by Downregulating the Phosphorylation Level of IκBα and Enhances. (tbdb.org)
- Notre recherche comprend une meilleure compréhension des voies métaboliques et des enzymes impliquées dans la biogenèse des lipides de l'enveloppe cellulaire chez Mycobacterium tuberculosis . (ipbs.fr)
- La tuberculose (TB), causée par Mycobacterium tuberculosis (Mtb), est l'une des causes majeures de décès dans le monde. (ipbs.fr)
- In WONDER, two tables are available, which list Mycobacterium species by region of the country, and antituberculosis drug susceptibility patterns of M. tuberculosis isolates by region of the country. (cdc.gov)
- For other nontuberculous mycobacteria (mycobacteria other than M. tuberculosis) reported in PHLIS, it is not possible to determine if an isolate was obtained from a patient with mycobacterial illness, or was simply colonizing a nonsterile site. (cdc.gov)
Nontuberculous Mycobacteria2
- Nontuberculous mycobacteria (NTM) are classified based on their growth rates. (medscape.com)
- Multifocal osteomyelitis caused by nontuberculous mycobacteria in patients with a genetic defect of the interferon-gamma receptor. (lu.se)
Organism2
- This organism was later named M. smegmatis. (wikipedia.org)
- M. smegmatis, which was previously considered a nonmotile organism, uses a sliding mechanism that allows it to move around its environment. (wikipedia.org)
Mycolicibacterium2
- Some species of the genus Mycobacterium have recently been renamed to Mycolicibacterium, so that M. smegmatis is now Mycolicibacterium smegmatis. (wikipedia.org)
- We hypothesized that changes in morphology may indicate changes in cell wall metabolism and identified an aceE gene mutant ( aceE -mut) which presented a deficient colony morphology on 7H10 agar by screening transposon mutagenesis in Mycolicibacterium smegmatis , basonym Mycobacterium smegmatis ( M. smegmatis ). (biomedcentral.com)
Species6
- Mycobacterium smegmatis is an acid-fast bacterial species in the phylum Actinomycetota and the genus Mycobacterium. (wikipedia.org)
- Mycobacterium smegmatis is useful for the research analysis of other Mycobacteria species in laboratory experiments. (wikipedia.org)
- The time and heavy infrastructure needed to work with pathogenic species prompted researchers to use M. smegmatis as a model for mycobacterial species. (wikipedia.org)
- species= Mycobacterium smegmatis str. (lbl.gov)
- The isolate was identifi ed by 16S infl ammatory processes within the wolinskyi is a rapidly growing my- rRNA gene amplifi cation of an 880-bp synovial tissue and the presence of ac- cobacterium that belongs to the M. smegmatis group, which includes M. smegmatis sensu stricto and 2 species described in 1999 ( M. goodii and M. wolinskyi ) ( 1 ). (cdc.gov)
- Some of these nonpathogenic mycobacteria are among the more common species processed in mycobacteriology laboratories. (cdc.gov)
Genome1
- To search for sequence similarities to the Mycobacterium phage L5 genome, click on the "GenBank: Z18946" link in the GenBank summary page for phage L5 (see Figure 3). (wikiversity.org)
Mycobacterial5
- Analysis of mycobacterial transcripts shows that the uspABC system is functionally expressed in mycobacteria as a contiguous reading frame. (birmingham.ac.uk)
- As cholesterol and branched-chain amino acid catabolism both generate propionyl- CoA , which is a toxic metabolite for mycobacteria , this interconnection suggests a compartmentalization to avoid dissemination of propionyl- CoA into the mycobacterial cytosol . (bvsalud.org)
- Mycobacterium smegmatis: The Vanguard of Mycobacterial Research. (nih.gov)
- Strategies for cryo-electron tomography of the mycobacterial cell envelope and its pore proteins and functional studies of porin MspA from Mycobacterium smegmatis. (mpg.de)
- INTERPRETIVE INFORMATION The PHLIS Mycobacterium module tracks mycobacterial isolates, not individual patients or specific cases of mycobacterial disease. (cdc.gov)
Strain3
- We observed that the colony morphology of aceE -mut was quite different, smaller and smoother on the solid culture medium than the wild-type (WT) strain during the transposon library screening of M. smegmatis . (biomedcentral.com)
- Results: Four compounds in this series significantly inhibit the growth and formation of biofilm by Mycobacterium smegmatis (mc(2) 155 strain) at 50 mu g/ml. (iisc.ac.in)
- This photomicrograph revealed the presence of a number of Mycobacterium smegmatis bacteria, strain 402, which were glowing green, having been processed using homologous antiserum, and the fluorescent antibody (FA) staining technique. (cdc.gov)
Biofilm formation3
- Notably, in contrast with the WT, which aggregates and forms biofilm, the aceE -mut lost its ability of growing aggregately and biofilm formation, which are two very important features of mycobacteria. (biomedcentral.com)
- Over all, this study indicates that aceE gene plays a significant role in the mycolic acid synthesis and affects the colony morphology, biofilm formation of M. smegmatis and bacteria invasion of macrophage. (biomedcentral.com)
- Conclusion: This finding is of significance as presently very few small molecules are known to inhibit biofilm formation in mycobacteria. (iisc.ac.in)
Porin2
- This has led scientists to experiment with other naturally occurring pore proteins such as Mycobacterium smegmatis porin A (MspA). (scienceblogs.com)
- The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients. (mpg.de)
Biosynthesis2
- The involvement of GlgE in glycogen biosynthesis was discovered twenty years ago when the phenotype of a temperature-sensitive Mycobacterium smegmatis mutation was rescued by the glgE gene. (jic.ac.uk)
- This study aimed to identify the functional role of aceE gene in cell wall biosynthesis in M. smegmatis . (biomedcentral.com)
Strains2
- citation needed] The genomes of multiple strains of M. smegmatis have been sequenced by TIGR and other laboratories, including the "wild-type" (mc2 155) and some antibiotic-resistant strains (4XR1/R2). (wikipedia.org)
- Herein, we have used a wide array of approaches to investigate the role of UspABC in Mycobacterium smegmatis by analysis of mutant strains that either lack the solute binding domain: ΔuspC or the entire transport complex: ΔuspABC. (birmingham.ac.uk)
Fortuitum2
- An antigenic analysis of the mycobacteria, Mycobacterium fortuitum, Myco. (eurekamag.com)
- Mycobacterium fortuitum complex is nonpigmented and includes the M fortuitum group and the Mycobacterium chelonae/abscessus group. (medscape.com)
Protein2
- For the M. smegmatis enzyme, the Vmax for S-adenosylmethionine formation was 1.30 μmol/min/mg protein and the Km for methionine and ATP was 288 μM and 76 μM respectively. (biomedcentral.com)
- The Recombinant Mycobacterium smegmatis GDP-mannose-dependent alpha- (1-2)-phosphatidylinositol mannosyltransferase, partial is a α- or alpha protein sometimes glycoprotein present in blood. (phosphatidylinositol.com)
Pathogenic1
- M. smegmatis is commonly used in work on the Mycobacterium genus due to it being a "fast grower" and non-pathogenic. (wikipedia.org)
Gene2
- By knocking out genes in the RD1 locus of M. smegmatis and testing efficiency of Snm secretion before and after gene knockout, specific genes can be identified as necessary for Snm secretion. (wikipedia.org)
- We have recently described the mpr gene of Mycobacterium smegmatis whose product confers resistance to mycobacteriophages L5 and D29 when overproduced. (pitt.edu)
Avium2
- Mycobacterium avium complex (MAC) and Mycobacterium scrofulaceum are associated with lymphadenitis in immunocompetent children. (medscape.com)
- Recurrent Mycobacterium avium osteomyelitis associated with a novel dominant interferon gamma receptor mutation. (lu.se)
Mutants2
- Previously, in our laboratory we showed that a deficiency of two Fpg/Nei glycosylases displayed no differences in survival of M. smegmatis mutants under oxidative stress and a 2-3 log difference was observed only when three or all four of the DNA glycosylases were inactivated. (wits.ac.za)
- Since in E.coli a double mutM and mutY mutant displayed an increased mutator phenotype compared to the individual mutants, it was plausible to investigate the role of MutY in combination with the Fpg/Nei family of DNA glycosylases in mycobacteria to understand whether these DNA glycosylases display overlapping and/or compensatory functions in dealing with oxidative damage. (wits.ac.za)
Pathway2
20231
- In 2023, researchers reported extracting from M. smegmatis a hydrogenase called Huc, which is highly efficient at oxidizing hydrogen gas-and thus creating an electric current-while also being insensitive to the presence of oxygen, which typically obstructs catalysis. (wikipedia.org)
Putative1
- Here, we focused on HsaC and HsaD, enzymes involved in two consecutive steps of cholesterol ring degradation and identified putative partners, using a BirA-based proximity-dependent biotin identification (BioID) approach in Mycobacterium smegmatis . (bvsalud.org)
Infection1
- Disseminated Mycobacterium peregrinum infection in a child with complete interferon-gamma receptor-1 deficiency. (lu.se)
Macrophages1
- These collectively form a thick, dense, poorly permeable cell wall, which not only allows mycobacteria to resist the dry environment and harmful chemicals but also allows it to reproduce in the macrophages [ 4 , 6 ]. (biomedcentral.com)
GenBank1
- Mycobacterium smegmatis mc 2 155 (NCBI GenBank, acc. (biomedcentral.com)
Susceptibility1
- A recent study further showed that a deficiency in M. smegmatis MutY glycosylase also displayed no notable susceptibility to oxidative stress or increase in mutagenesis. (wits.ac.za)
Lipoarabinomannan1
- Lipoarabinomannan (LAM) is a glycolipid Toll-Like Receptor-2 ligand found on the cell wall of mycobacteria. (lynchburg.edu)
Enzyme1
- The kinetic properties of the M. smegmatis enzyme were also similar to known prokaryotic methionine adenosyltransferases. (biomedcentral.com)
Commonly1
Survival1
- The integrity of cell wall structure is highly significant for the in vivo survival of mycobacteria. (biomedcentral.com)
Complex1
- Mycobacterium smegmatis is readily cultivatable in most synthetic or complex laboratory media, where it can form visible colonies in 3-5 days. (wikipedia.org)
Identification1
- Proximity-dependent biotin identification links cholesterol catabolism with branched-chain amino acid degradation in Mycobacterium smegmatis. (bvsalud.org)
Laboratory3
- M. smegmatis is a simple model that is easy to work with, i.e., with a fast doubling time and only requires a biosafety level 1 laboratory. (wikipedia.org)
- Laboratory data showed a normal of Mycobacterium spp. (cdc.gov)
- The operation of the PHLIS Mycobacterium module is facilitated by agreements between CDC and the Association of State and Territorial Public Health Laboratory Directors on system content, protocols for formatting and transmitting data, and standard case definitions. (cdc.gov)
Cell wall1
- Although it hasn't been determined exactly how this mechanism works, the surface properties of the unique cell wall (Figure 1) of M. smegmatis have been found to play a role. (wikipedia.org)
Laboratories1
- file contains data on mycobacteria isolated and identified in state public health laboratories. (cdc.gov)
Citation1
- citation needed] Like many other bacteria, M. smegmatis is known to use the trace levels of hydrogen in the atmosphere as an energy source. (wikipedia.org)
Unique2
- These compounds are unique in the sense that they are more potent against Mycobacterium smegmatis in the biofilm state compared to the planktonic form. (iisc.ac.in)
- L'enveloppe mycobactérienne est unique dans le monde bactérien, tant par sa composition riche en composés structurellement exotiques, que par son agencement, avec notamment la présence d'une membrane externe et d'une membrane interne. (ipbs.fr)
Found2
- Subsequent to this, Alvarez and Tavel found organisms similar to that described by Lustgarten also in normal genital secretions (smegma). (wikipedia.org)
- Atypical mycobacteria are obligate aerobes that can be found in the environment in soil, water, vegetables, and even in domestic animals and dairy products. (medscape.com)
Molecular1
- However, the molecular partners of cholesterol catabolism remain elusive in mycobacteria . (bvsalud.org)
Role1
- Mycobacteria possess four copies of the Fpg/Nei glycosylases with the Fpg homologue displaying an overlapping role with MutM in the GO system. (wits.ac.za)