• genome
  • Since 2013, the development of the CRISPR/Cas9 technology, based on a prokaryotic viral defense system, has also allowed for the editing of the genome, and mutagenesis may be performed in vivo with relative ease. (wikipedia.org)
  • These nucleases create site-specific double-strand breaks (DSBs) at desired locations in the genome. (wikipedia.org)
  • The primary advantage of this technique is its ability to eliminate any foreign DNA from the genome after the mutagenesis process. (wikipedia.org)
  • Since 2013, development of the CRISPR/Cas9 technology, based on a prokaryotic viral defense system, has also allowed for the editing or mutagenesis of the genome in vivo. (wikipedia.org)
  • specificity
  • Since the glycoprotein hormones contain a common α-subunit, and since both subunits are believed to participate in receptor binding (6, 7), interesting questions and models can be developed regarding subunit contact sites and hormone receptor contact sites, particularly those responsible for conferring specificity. (springer.com)
  • gene
  • This synthetic primer contains the desired mutation and is complementary to the template DNA around the mutation site so it can hybridize with the DNA in the gene of interest. (wikipedia.org)
  • The gene thus copied contains the mutated site, and is then introduced into a host cell as a vector and cloned. (wikipedia.org)
  • Our customized mutagenesis services provide a fail-safe approach to obtain mutant constructs quickly, with 100% accuracy, thus eliminating the possibility of undesired mutations in your gene. (genewiz.com)
  • To test our previous hypothesis that the segment between amino acid position 63 to 73 of the H-2D d antigen forms a major allo-antigenic site, mutations were introduced into the H-2L d gene in a sequential fashion, which replaced the codons for amino acid position 63, 65, 66, 70 and 73 of the H-2L d antigen with those of the H-2D d antigen. (springer.com)
  • Thus if one is able to create a DSB at a desired gene in multiple samples, it is very likely that mutations will be generated at that site in some of the treatments because of errors created by the NHEJ infidelity. (wikipedia.org)
  • A variation of this method for integrating non-biased mutations in a gene is the Sequence Saturation Mutagenesis. (wikipedia.org)
  • proteins
  • Mutagenesis in the laboratory is an important technique whereby DNA mutations are deliberately engineered to produce mutant genes, proteins, strains of bacteria, or other genetically modified organisms. (wikipedia.org)
  • Analysis
  • Despite the lack of evidence suggesting an exact mechanism for the cleavage of DNA by HindIII, site-mutagenesis analysis coupled with more detailed studies of metal ion-mediated catalysis in EcoRV have led to the following proposed catalytic mechanism. (wikipedia.org)
  • homology
  • In order to develop a more "human-like" uricase for therapeutic use, exon replacement/restoration and site-directed mutagenesis were performed to obtain porcine-human uricase with higher homology to deduced human uricase (dHU) and increased uricolytic activity. (mdpi.com)
  • One of the known DSB repair pathways that are essentially functional in all organisms[citation needed] are the non-homologous end joining (NHEJ) and homology directed repair (HDR). (wikipedia.org)
  • molecules
  • The cofactor Mg2+ is believed to bind water molecules and carry them to the catalytic sites of the enzymes, among other cations. (wikipedia.org)
  • mouse
  • Insertional mutagenesis using transposons, retrovirus such as mouse mammary tumor virus and murine leukemia virus may be used to identify genes involved in carcinogenesis and to understand the biological pathways of specific cancer. (wikipedia.org)
  • enzymes
  • DOI: 10.1002/anie.201007896 Enzymatic Halogenation Changing the Regioselectivity of the Tryptophan 7-Halogenase PrnA by Site-Directed Mutagenesis** Alexander Lang, Stefan Polnick, Tristan Nicke, Peter William, Eugenio P. Patallo, James H. Naismith, and Karl-Heinz van Pe* Dedicated to Professor Franz Lingens on the occasion of his 85th birthday For many years, haloperoxidases were the only type of halogenating enzymes known. (docme.ru)
  • The structure of some enzymes are very well characterized, however, the function of some component of the active site is poorly understood. (wikipedia.org)
  • Type I restriction enzymes recognize specific sequences, but cleave DNA randomly at sites other than their recognition site whereas type II restriction enzymes cleave only at their specific recognition site. (wikipedia.org)
  • approach
  • One approach of this technique is to excise a portion of DNA and replaced with a library of sequences containing all possible combinations at the desired mutation sites. (wikipedia.org)
  • specific
  • Despite the fact that this residue is most likely responsible for the unwinding of DNA and coordination to water rather than direct interaction with the attacking nucleophile, its specific function is unknown. (wikipedia.org)
  • Many researchers seek to introduce selected changes to DNA in a site-specific manner. (wikipedia.org)
  • active
  • 1, Haloperoxidases (hemeand vanadium-containing) catalyze the formation of hypohalous acids,[3, which diffuse out of the active site and then react with substrate. (docme.ru)
  • Perhydrolases catalyze the formation of peracids, which react outside of the active site with halide ions to form hypohalous acids. (docme.ru)
  • Active site of PrnA showing the binding of the substrate tryptophan. (docme.ru)
  • Defining the active site structure. (wikipedia.org)
  • A number of important active sites are still poorly defined. (wikipedia.org)
  • Understanding the active site function. (wikipedia.org)
  • function
  • As a result of the site-mutagenesis experiments previously outlined, it is thus proposed that Lys-125, Asp-123, and Asp-108 of HindIII function similarly to Lys-92, Asp-90, and Asp-74 in EcoRV, respectively. (wikipedia.org)
  • shown
  • NHEJ is error-prone, and has been shown to cause mutations at the repair site in approximately 50% of DSB in mycobacteria, and also its low fidelity has been linked to mutational accumulation in leukemias. (wikipedia.org)