• genome
  • Since 2013, the development of the CRISPR/Cas9 technology, based on a prokaryotic viral defense system, has also allowed for the editing of the genome, and mutagenesis may be performed in vivo with relative ease. (wikipedia.org)
  • These nucleases create site-specific double-strand breaks (DSBs) at desired locations in the genome. (wikipedia.org)
  • The primary advantage of this technique is its ability to eliminate any foreign DNA from the genome after the mutagenesis process. (wikipedia.org)
  • Since 2013, development of the CRISPR/Cas9 technology, based on a prokaryotic viral defense system, has also allowed for the editing or mutagenesis of the genome in vivo. (wikipedia.org)
  • enzyme
  • The coenzymes bind to the active site of an enzyme to promote catalysis. (wikipedia.org)
  • HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis. (wikipedia.org)
  • The segment may be at an enzyme active site, or sequences that have structural significance or immunogenic property. (wikipedia.org)
  • specificity
  • Since the glycoprotein hormones contain a common α-subunit, and since both subunits are believed to participate in receptor binding (6, 7), interesting questions and models can be developed regarding subunit contact sites and hormone receptor contact sites, particularly those responsible for conferring specificity. (springer.com)
  • gene
  • This synthetic primer contains the desired mutation and is complementary to the template DNA around the mutation site so it can hybridize with the DNA in the gene of interest. (wikipedia.org)
  • The gene thus copied contains the mutated site, and is then introduced into a host cell as a vector and cloned. (wikipedia.org)
  • restriction site
  • These protocols are used if you want to change one to three consecutive bases in a sequence (like removing a restriction site or changing an amino acid). (openwetware.org)
  • While restriction enzymes cleave at specific DNA sequences, they are first required to bind non-specifically with the DNA backbone before localizing to the restriction site. (wikipedia.org)
  • homology
  • In order to develop a more "human-like" uricase for therapeutic use, exon replacement/restoration and site-directed mutagenesis were performed to obtain porcine-human uricase with higher homology to deduced human uricase (dHU) and increased uricolytic activity. (mdpi.com)
  • One of the known DSB repair pathways that are essentially functional in all organisms[citation needed] are the non-homologous end joining (NHEJ) and homology directed repair (HDR). (wikipedia.org)
  • mouse
  • Insertional mutagenesis using transposons, retrovirus such as mouse mammary tumor virus and murine leukemia virus may be used to identify genes involved in carcinogenesis and to understand the biological pathways of specific cancer. (wikipedia.org)
  • active
  • 1, Haloperoxidases (hemeand vanadium-containing) catalyze the formation of hypohalous acids,[3, which diffuse out of the active site and then react with substrate. (docme.ru)
  • Perhydrolases catalyze the formation of peracids, which react outside of the active site with halide ions to form hypohalous acids. (docme.ru)
  • Active site of PrnA showing the binding of the substrate tryptophan. (docme.ru)
  • Defining the active site structure. (wikipedia.org)
  • A number of important active sites are still poorly defined. (wikipedia.org)
  • Understanding the active site function. (wikipedia.org)
  • The structure of some enzymes are very well characterized, however, the function of some component of the active site is poorly understood. (wikipedia.org)
  • enzymes
  • DOI: 10.1002/anie.201007896 Enzymatic Halogenation Changing the Regioselectivity of the Tryptophan 7-Halogenase PrnA by Site-Directed Mutagenesis** Alexander Lang, Stefan Polnick, Tristan Nicke, Peter William, Eugenio P. Patallo, James H. Naismith, and Karl-Heinz van Pe* Dedicated to Professor Franz Lingens on the occasion of his 85th birthday For many years, haloperoxidases were the only type of halogenating enzymes known. (docme.ru)
  • Type I restriction enzymes recognize specific sequences, but cleave DNA randomly at sites other than their recognition site whereas type II restriction enzymes cleave only at their specific recognition site. (wikipedia.org)
  • approach
  • One approach of this technique is to excise a portion of DNA and replaced with a library of sequences containing all possible combinations at the desired mutation sites. (wikipedia.org)
  • specific
  • Despite the fact that this residue is most likely responsible for the unwinding of DNA and coordination to water rather than direct interaction with the attacking nucleophile, its specific function is unknown. (wikipedia.org)
  • Many researchers seek to introduce selected changes to DNA in a site-specific manner. (wikipedia.org)
  • important
  • Upon comparing the amino acid sequences of mammalian brain and S. cerevisiae β-tubulin we observed five differences at sites in β-tubulin purported to be important in paclitaxel binding. (pubmedcentralcanada.ca)
  • function
  • As a result of the site-mutagenesis experiments previously outlined, it is thus proposed that Lys-125, Asp-123, and Asp-108 of HindIII function similarly to Lys-92, Asp-90, and Asp-74 in EcoRV, respectively. (wikipedia.org)