• markers
  • Cloned SP cells cultured in medium with fibroblast growth factor-2 lost ABCG2 and PAX6 expression and upregulated several molecular markers of keratocytes, including keratocan, aldehyde dehydrogenase 3A1, and keratan sulfate. (mendeley.com)
  • Cloned corneal SP cells under chondrogenic conditions produced matrix staining with toluidine blue and expressed cartilage-specific markers: collagen II, cartilage oligomatrix protein, and aggrecan. (mendeley.com)
  • Pericytes are multipotent cells that are heterogeneous in their origin, function, morphology and surface markers. (springer.com)
  • cell-permeable molecule called Cardiogenol C. We founded that Cardiogenol C could induce HBPCs to express transcription factors GATA4 Nkx2.5 and Tbx5 which are early specific markers for pre-cardiomyogenic cells. (elevateblogconference.com)
  • During this differentiation process, keratinocytes permanently withdraw from the cell cycle, initiate expression of epidermal differentiation markers, and move suprabasally as they become part of the stratum spinosum, stratum granulosum and eventually become corneocytes in the stratum corneum. (wikipedia.org)
  • Stem cells can also be isolated by their possession of a distinctive set of cell surface markers. (wikipedia.org)
  • germ-line
  • Testes-derived male germ-line stem cells exhibit spermatogenic potential or multipotency depending on the culture conditions used for their isolation and in vitro maintenance. (springer.com)
  • This chapter describes the biogenesis and functions of miRNAs in the context of testes-derived male germ-line stem cells and discusses their potential application in distinguishing the unipotent GS cells from the multipotent maGS cells. (springer.com)
  • Jung YH, Gupta MK, Shin JY, Uhm SJ, Lee HT (2010b) MicroRNA signature in testes-derived male germ-line stem cells. (springer.com)
  • In Drosophila, zygotic transcription of Vasa occurs at pole cells, and stays germ-line specific throughout the life of the organism. (wikipedia.org)
  • In P. hawaienis, Vasa transcript is uniformly distributed in the embryo and is localized depending on the stabilization of the 3'UTR (Untranslated Region) to the germ line cells. (wikipedia.org)
  • proliferation
  • The mechanisms of proliferation and differentiation of hematopoietic multipotent stem cells. (nii.ac.jp)
  • The physiological roles of these factors in the regulatory mechanisms of stem cell proliferation are still unknown. (nii.ac.jp)
  • Proliferation capacity, self-renewal capacity, and ability for multipotent differentiation were analyzed by BrdU labeling, flow cytometry, reverse transcriptase-polymerase chain reaction, immunocytochemistry, and in vivo transplantation. (arvojournals.org)
  • Those stem cells and their differentiated progeny are organized into columns named epidermal proliferation units. (wikipedia.org)
  • Proliferation of these cell types were characterized in further experiments that were reported in articles in Development and the Journal of Neuroscience. (wikipedia.org)
  • Based on the observation that these cells require PDGF for their proliferation and expansion, the expression of the alpha receptor for platelet-derived growth factor (Pdgfra) was used to search for the in vivo correlates of the A2B5+ cells, which led to the discovery of a unique population of Pdgfra+ cells in the CNS whose appearance and distribution were consistent with those of developing oligodendrocytes. (wikipedia.org)
  • After the committed progenitor cells exit the germinal zones, they begin to express NG2 and Pdgfra and expand by local proliferation and migration and eventually occupy the entire CNS parenchyma. (wikipedia.org)
  • endoderm
  • giving rise to any of the three germ layers of human (endoderm, ectoderm or mesoderm) as well as extra embryonic cells which include cytotrophoblast layer and a syncytiotrophoblast layer of the placenta . (medcraveonline.com)
  • It is important to note here that the developing dorsal and ventral buds are characterized as endoderm, and it isn't until the expression of Pdx1 (specification of endoderm to a stem cell multipotent state) that the endoderm to pancreatic progenitor transition occurs. (wikipedia.org)
  • glial cells
  • In this capacity, glial cells provide multiple functions to both the central nervous system (CNS) and the peripheral nervous system (PNS). (wikipedia.org)
  • Understanding the mechanisms in which gliogenesis is regulated provides the potential to harness the ability to control the fate of glial cells and, consequently, the ability to reverse neurodegenerative diseases. (wikipedia.org)
  • Proneural factors are expressed in high concentrations during times in which glial cells are not to form or neuron development is needed. (wikipedia.org)
  • Independently, Stallcup and colleagues generated an antiserum that recognized a group of rat brain tumor cell lines, which exhibited properties that were intermediate between those of typical neurons and glial cells. (wikipedia.org)
  • BrdU
  • Using stain-retaining assay (with bromodeoxyuridine, or BrdU), a low-cycling cell population was found in the papillary region, which was able to divide rapidly to repair the damaged caused by transcient renal ischemia. (wikipedia.org)
  • During S-phase cells are treated with bromodeoxyuridine (BrdU) which is then incorporated into their nascent DNA, acting as a substitute for thymidine. (wikipedia.org)
  • self-renewal
  • The continuous self-renewal and differentiating ability of stem cells has created modern research emphasis on it, especially in relation to its therapeutic use . (medcraveonline.com)
  • The classical definition of a stem cell requires that it possesses two properties: Self-renewal: the ability to go through numerous cycles of cell division while maintaining the undifferentiated state. (wikipedia.org)
  • Unipotent cells can produce only one cell type, their own, but have the property of self-renewal, which distinguishes them from non-stem cells (e.g. progenitor cells, which cannot self-renew). (wikipedia.org)
  • autologous
  • Intracoronary autologous bone-marrow cell transfer after myocardial infarction: the BOOST randomised controlled clinical trial," The Lancet , vol. 364, no. 9429, pp. 141-148, 2004. (hindawi.com)
  • Autologous bone marrow-derived stem-cell transfer in patients with ST-segment elevation myocardial infarction: double-blind, randomised controlled trial," The Lancet , vol. 367, no. 9505, pp. 113-121, 2006. (hindawi.com)
  • Comparison of allogeneic vs autologous bone marrow-derived mesenchymal stem cells delivered by transendocardial injection in patients with ischemic cardiomyopathy: the POSEIDON randomized trial," Journal of the American Medical Association , vol. 308, no. 22, pp. 2369-2379, 2012. (hindawi.com)
  • There are three known accessible sources of autologous adult stem cells in humans: Bone marrow, which requires extraction by harvesting, that is, drilling into bone (typically the femur or iliac crest). (wikipedia.org)
  • Of all stem cell types, autologous harvesting involves the least risk. (wikipedia.org)
  • By definition, autologous cells are obtained from one's own body, just as one may bank his or her own blood for elective surgical procedures. (wikipedia.org)
  • Embryonic cell lines and autologous embryonic stem cells generated through somatic cell nuclear transfer or dedifferentiation have also been proposed as promising candidates for future therapies. (wikipedia.org)
  • functional
  • The next challenge will be to demonstrate the functional utility of these cells, both in vitro and in preclinical models of bone and vascular diseases. (hindawi.com)
  • progenitors
  • The differential of pancreatic progenitors from hepatic progenitors is also notable, as Hhex1 (Hematopoietically expressed homeobox gene) is responsible for the origination of pancreatic progenitor cells. (wikipedia.org)
  • vivo
  • Ex vivo NIR TD optical imaging results showed a systemic distribution of labelled cells. (duhnnae.com)
  • Using in vivo lineage tracing techniques, Lgr5+ cells were found to contribute to the nephron, specifically to the ascending limb of the loop of Henle and the distal convoluted tubule. (wikipedia.org)
  • As noted for receptor-proteins, in vivo interactions among different growth factor responsible for gliogenesis and other cell fates produce very different roles than when isolated. (wikipedia.org)
  • Cells of interest are cultured either in vivo or in vitro. (wikipedia.org)
  • lineage
  • d lineage, and that IL-3 acted not only on these two subpopulation but also on more primitive stem cell population which was resistant to 5FU. (nii.ac.jp)
  • Cardiopoietic stem cell therapy in heart failure the C-CURE multicenter randomized trial with lineage-specified biologics," Journal of the American College of Cardiology , 2013. (hindawi.com)
  • The megakaryocyte develops through the following lineage: CFU-Me (pluripotential hemopoietic stem cell or hemocytoblast) → megakaryoblast → promegakaryocyte → megakaryocyte. (wikipedia.org)
  • Genetic lineage tracing experiments have been performed by various research groups to show that the cell clusters originating from the developing foregut express a transcription factor called PDX1 (Pancreatic and duodenal homeobox 1). (wikipedia.org)
  • Distinct
  • In the forebrain, three regionally distinct sources have been shown to generate polydendrocytes sequentially: an early ventral source from the medial ganglionic eminence marked by Nkx2.1, followed by progenitor cells in the lateral ganglionic eminence marked by Gsh2, and finally the dorsal neocortical germinal zone marked by Emx1. (wikipedia.org)
  • precursors
  • Finally, microglial cells are derived from glial precursors and carry-out macrophage like properties to remove cellular and foreign debris within the central nervous system ref. (wikipedia.org)
  • pancreas
  • The Adult Mouse and Human Pancreas Contain Rare Multipotent Stem Cells" by Simon R. Smukler, Margot E. Arntfield et al. (uwindsor.ca)
  • Based on his experiments with human cadaveric pancreata, van der Kooy holds theorizes that there exists an adult stem cell in the pancreas. (wikipedia.org)
  • The fused and developed pancreas consists of pancreatic enzyme secreting cells (exocrine cells), digestive enzyme transporting cells (ductal cells) and hormone producing cells (endocrine cells). (wikipedia.org)
  • These endocrine cells develop in discrete areas within the pancreas known as the islets. (wikipedia.org)
  • Notch signaling has been shown to regulate the number of exocrine and endocrine cells in the pancreas, but not without the presence of Pdx1. (wikipedia.org)
  • Protocols
  • Single-cell sequencing protocols were foundational in the development of this technique, but they differ in several aspects. (wikipedia.org)
  • Transplantation
  • However, studies of tlie action of these cytokines on hemopoietic stem cells are important research fields in regarding to clinical uses such as in votro expansion for transplantation. (nii.ac.jp)
  • Transcoronary transplantation of progenitor cells after myocardial infarction," The New England Journal of Medicine , vol. 355, no. 12, pp. 1222-1232, 2006. (hindawi.com)
  • Transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction (TOPCARE-AMI): final 5-year results suggest long-term safety and efficacy," Clinical Research in Cardiology , vol. 100, no. 10, pp. 925-934, 2011. (hindawi.com)
  • In particular, testicular transplantation of GS cells with contaminating maGS cells may result in teratoma formation. (springer.com)
  • Humans
  • In humans, it is estimated that keratinocytes turnover from stem cells to desquamation every 40-56 days. (wikipedia.org)
  • The vasa gene, is essential for germ cell development and was first identified in Drosophila melanogaster, but has since been found to be conserved in a variety of vertebrates and invertebrates including humans. (wikipedia.org)
  • Contribute
  • The next step for the researchers is to find out how the cells are reprogrammed to form blood and to understand why the MAPCs didn't contribute to more general repair in the irradiated mice. (bionews.org.uk)
  • epithelial
  • Acloque H, Adams MS, Fishwick K, Bronner-Fraser M, Nieto MA (2009) Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease. (springer.com)
  • It has been reported that endogenous kidney tubular renal epithelial cells can be dedifferentiated into induced pluripotent stem cells by the treatment of only two factors - Oct4 and Sox2. (wikipedia.org)
  • Dr. Gordon's laboratory has investigated epithelial cell interaction with human-associated pathogens, including uropathogenic Escherichia coli, Helicobacter pylori, and Listeria monocytogenes. (wikipedia.org)
  • Syndecan-1 expression in prostate cancer and the role of p27 in primary prostate epithelial cell transformation. (wikipedia.org)
  • abcg2
  • The SP cells exhibited clonal growth and continued to express ABCG2 and also PAX6, product of a homeobox gene not expressed in adult keratocytes. (mendeley.com)
  • Department of Anatomy
  • In 1981, he became an Assistant Professor, was promoted to Associate Professor in 1986, and has served as Professor in the Department of Anatomy and Cell Biology at the University of Toronto from 1991 until 2002, when he became a Professor in the Department of Medical Genetics and Microbiology. (wikipedia.org)