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  • adult
  • These results represent the first demonstration of adult stem cells with the potential to be differentiated into different types of ECs in vitro and in vivo and provide a useful human model to study arteriovenous specification. (cun.es)
  • These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. (atcc.org)
  • human
  • ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. (atcc.org)
  • It is best to use caution when handling any human cells. (atcc.org)
  • We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. (atcc.org)
  • germ-line
  • Testes-derived male germ-line stem cells exhibit spermatogenic potential or multipotency depending on the culture conditions used for their isolation and in vitro maintenance. (springer.com)
  • This chapter describes the biogenesis and functions of miRNAs in the context of testes-derived male germ-line stem cells and discusses their potential application in distinguishing the unipotent GS cells from the multipotent maGS cells. (springer.com)
  • Jung YH, Gupta MK, Shin JY, Uhm SJ, Lee HT (2010b) MicroRNA signature in testes-derived male germ-line stem cells. (springer.com)
  • In Drosophila, zygotic transcription of Vasa occurs at pole cells, and stays germ-line specific throughout the life of the organism. (wikipedia.org)
  • In P. hawaienis, Vasa transcript is uniformly distributed in the embryo and is localized depending on the stabilization of the 3'UTR (Untranslated Region) to the germ line cells. (wikipedia.org)
  • autologous
  • Cell Surgical Network is offering patient funded research to treat individual patients with their own autologous stem cells and CSN is not involved in the use or manufacture of any investigational drugs. (gulfcoaststemcell.com)
  • CSN does not claim that any applications, or potential applications, using these autologous stem cell therapies are approved by the FDA, or are even effective. (gulfcoaststemcell.com)
  • ATTENTION: If you have ANY concern with our autologous stem cell therapy product, methods, website, or technique and think we may be violating any U.S. law, please contact us so that we can investigate the matter or concern immediately. (gulfcoaststemcell.com)
  • Intracoronary autologous bone-marrow cell transfer after myocardial infarction: the BOOST randomised controlled clinical trial," The Lancet , vol. 364, no. 9429, pp. 141-148, 2004. (hindawi.com)
  • Autologous bone marrow-derived stem-cell transfer in patients with ST-segment elevation myocardial infarction: double-blind, randomised controlled trial," The Lancet , vol. 367, no. 9505, pp. 113-121, 2006. (hindawi.com)
  • Comparison of allogeneic vs autologous bone marrow-derived mesenchymal stem cells delivered by transendocardial injection in patients with ischemic cardiomyopathy: the POSEIDON randomized trial," Journal of the American Medical Association , vol. 308, no. 22, pp. 2369-2379, 2012. (hindawi.com)
  • There are three known accessible sources of autologous adult stem cells in humans: Bone marrow, which requires extraction by harvesting, that is, drilling into bone (typically the femur or iliac crest). (wikipedia.org)
  • Of all stem cell types, autologous harvesting involves the least risk. (wikipedia.org)
  • By definition, autologous cells are obtained from one's own body, just as one may bank his or her own blood for elective surgical procedures. (wikipedia.org)
  • mammalian
  • The team isolated mesenchymal stem cells from mammalian bone marrow and grew them through at least 80 generations in the lab, they then used these cells to repopulate the damaged blood system. (bionews.org.uk)
  • Of note, Derek's lab produced the first report of stem cells in the adult mammalian eye, published in 2000 in Science. (wikipedia.org)
  • Pancreatic progenitor cells have been shown to arise from cells originating from the developing foregut during mammalian development. (wikipedia.org)
  • Polydendrocytes (also known as NG2 cells, NG2 glia, or oligodendrocyte progenitor cells) are process-bearing glial cells (neuroglia) in the mammalian central nervous system (CNS) that are identified by the expression of the NG2 chondroitin sulfate proteoglycan (CSPG4) and the alpha receptor for platelet-derived growth factor (PDGFRA). (wikipedia.org)
  • proliferation
  • The mechanisms of proliferation and differentiation of hematopoietic multipotent stem cells. (nii.ac.jp)
  • The physiological roles of these factors in the regulatory mechanisms of stem cell proliferation are still unknown. (nii.ac.jp)
  • Proliferation capacity, self-renewal capacity, and ability for multipotent differentiation were analyzed by BrdU labeling, flow cytometry, reverse transcriptase-polymerase chain reaction, immunocytochemistry, and in vivo transplantation. (arvojournals.org)
  • Those stem cells and their differentiated progeny are organized into columns named epidermal proliferation units. (wikipedia.org)
  • Proliferation of these cell types were characterized in further experiments that were reported in articles in Development and the Journal of Neuroscience. (wikipedia.org)
  • Based on the observation that these cells require PDGF for their proliferation and expansion, the expression of the alpha receptor for platelet-derived growth factor (Pdgfra) was used to search for the in vivo correlates of the A2B5+ cells, which led to the discovery of a unique population of Pdgfra+ cells in the CNS whose appearance and distribution were consistent with those of developing oligodendrocytes. (wikipedia.org)
  • After the committed progenitor cells exit the germinal zones, they begin to express NG2 and Pdgfra and expand by local proliferation and migration and eventually occupy the entire CNS parenchyma. (wikipedia.org)
  • endoderm
  • giving rise to any of the three germ layers of human (endoderm, ectoderm or mesoderm) as well as extra embryonic cells which include cytotrophoblast layer and a syncytiotrophoblast layer of the placenta . (medcraveonline.com)
  • It is important to note here that the developing dorsal and ventral buds are characterized as endoderm, and it isn't until the expression of Pdx1 (specification of endoderm to a stem cell multipotent state) that the endoderm to pancreatic progenitor transition occurs. (wikipedia.org)
  • glial cells
  • Independently, Stallcup and colleagues generated an antiserum that recognized a group of rat brain tumor cell lines, which exhibited properties that were intermediate between those of typical neurons and glial cells. (wikipedia.org)
  • In this capacity, glial cells provide multiple functions to both the central nervous system (CNS) and the peripheral nervous system (PNS). (wikipedia.org)
  • Understanding the mechanisms in which gliogenesis is regulated provides the potential to harness the ability to control the fate of glial cells and, consequently, the ability to reverse neurodegenerative diseases. (wikipedia.org)
  • Proneural factors are expressed in high concentrations during times in which glial cells are not to form or neuron development is needed. (wikipedia.org)
  • distinct
  • Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression. (gulfcoaststemcell.com)
  • In the forebrain, three regionally distinct sources have been shown to generate polydendrocytes sequentially: an early ventral source from the medial ganglionic eminence marked by Nkx2.1, followed by progenitor cells in the lateral ganglionic eminence marked by Gsh2, and finally the dorsal neocortical germinal zone marked by Emx1. (wikipedia.org)
  • self-renewal
  • The continuous self-renewal and differentiating ability of stem cells has created modern research emphasis on it, especially in relation to its therapeutic use . (medcraveonline.com)
  • The classical definition of a stem cell requires that it possesses two properties: Self-renewal: the ability to go through numerous cycles of cell division while maintaining the undifferentiated state. (wikipedia.org)
  • Unipotent cells can produce only one cell type, their own, but have the property of self-renewal, which distinguishes them from non-stem cells (e.g. progenitor cells, which cannot self-renew). (wikipedia.org)
  • lineage
  • d lineage, and that IL-3 acted not only on these two subpopulation but also on more primitive stem cell population which was resistant to 5FU. (nii.ac.jp)
  • Mesodermal lineage induction of PLA cells and clones resulted in the expression of multiple lineage-specific genes and proteins. (gulfcoaststemcell.com)
  • Cardiopoietic stem cell therapy in heart failure the C-CURE multicenter randomized trial with lineage-specified biologics," Journal of the American College of Cardiology , 2013. (hindawi.com)
  • Using in vivo lineage tracing techniques, Lgr5+ cells were found to contribute to the nephron, specifically to the ascending limb of the loop of Henle and the distal convoluted tubule. (wikipedia.org)
  • The megakaryocyte develops through the following lineage: CFU-Me (pluripotential hemopoietic stem cell or hemocytoblast) → megakaryoblast → promegakaryocyte → megakaryocyte. (wikipedia.org)
  • Genetic lineage tracing experiments have been performed by various research groups to show that the cell clusters originating from the developing foregut express a transcription factor called PDX1 (Pancreatic and duodenal homeobox 1). (wikipedia.org)
  • pancreas
  • It has been seen in the developing embryo at stages E9.0 to E9.5 that there are a cluster of cells which give rise to the pancreas. (wikipedia.org)
  • The fused and developed pancreas consists of pancreatic enzyme secreting cells (exocrine cells), digestive enzyme transporting cells (ductal cells) and hormone producing cells (endocrine cells). (wikipedia.org)
  • These endocrine cells develop in discrete areas within the pancreas known as the islets. (wikipedia.org)
  • Notch signaling has been shown to regulate the number of exocrine and endocrine cells in the pancreas, but not without the presence of Pdx1. (wikipedia.org)
  • Cellular
  • Finally, microglial cells are derived from glial precursors and carry-out macrophage like properties to remove cellular and foreign debris within the central nervous system ref. (wikipedia.org)
  • functional
  • The next challenge will be to demonstrate the functional utility of these cells, both in vitro and in preclinical models of bone and vascular diseases. (hindawi.com)
  • vitro
  • In contrast to research using biochemistry, genetics, or molecular biology, where mutagenesis can provide a new version of the organism, cell, or biomolecule of interest, chemical biology probes systems in vitro and in vivo with small molecules that have been designed for a specific purpose or identified on the basis of biochemical or cell-based screening (see chemical genetics). (wikipedia.org)
  • The manipulated cell is cultured in vitro to develop to a specific embryonic phase, is then transferred to a recipient female. (wikipedia.org)
  • progenitors
  • The differential of pancreatic progenitors from hepatic progenitors is also notable, as Hhex1 (Hematopoietically expressed homeobox gene) is responsible for the origination of pancreatic progenitor cells. (wikipedia.org)
  • precursors
  • In one series of studies on the development and origin of oligodendrocytes in the rodent CNS, a population of immature cells that appeared to be precursors to oligodendrocytes was identified by the expression of the GD3 ganglioside. (wikipedia.org)
  • BrdU
  • Using stain-retaining assay (with bromodeoxyuridine, or BrdU), a low-cycling cell population was found in the papillary region, which was able to divide rapidly to repair the damaged caused by transcient renal ischemia. (wikipedia.org)
  • Transplantation
  • However, studies of tlie action of these cytokines on hemopoietic stem cells are important research fields in regarding to clinical uses such as in votro expansion for transplantation. (nii.ac.jp)
  • Transcoronary transplantation of progenitor cells after myocardial infarction," The New England Journal of Medicine , vol. 355, no. 12, pp. 1222-1232, 2006. (hindawi.com)
  • Transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction (TOPCARE-AMI): final 5-year results suggest long-term safety and efficacy," Clinical Research in Cardiology , vol. 100, no. 10, pp. 925-934, 2011. (hindawi.com)
  • In particular, testicular transplantation of GS cells with contaminating maGS cells may result in teratoma formation. (springer.com)
  • Humans
  • In humans, it is estimated that keratinocytes turnover from stem cells to desquamation every 40-56 days. (wikipedia.org)
  • The vasa gene, is essential for germ cell development and was first identified in Drosophila melanogaster, but has since been found to be conserved in a variety of vertebrates and invertebrates including humans. (wikipedia.org)
  • epithelial
  • Acloque H, Adams MS, Fishwick K, Bronner-Fraser M, Nieto MA (2009) Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease. (springer.com)
  • It has been reported that endogenous kidney tubular renal epithelial cells can be dedifferentiated into induced pluripotent stem cells by the treatment of only two factors - Oct4 and Sox2. (wikipedia.org)
  • Dr. Gordon's laboratory has investigated epithelial cell interaction with human-associated pathogens, including uropathogenic Escherichia coli, Helicobacter pylori, and Listeria monocytogenes. (wikipedia.org)
  • Syndecan-1 expression in prostate cancer and the role of p27 in primary prostate epithelial cell transformation. (wikipedia.org)
  • abcg2
  • The ABCG2-expressing cell population was isolated as a side population (SP) by cell sorting after exposure to Hoechst 33342 dye. (mendeley.com)
  • The SP cells exhibited clonal growth and continued to express ABCG2 and also PAX6, product of a homeobox gene not expressed in adult keratocytes. (mendeley.com)
  • Department of Anatomy
  • In 1981, he became an Assistant Professor, was promoted to Associate Professor in 1986, and has served as Professor in the Department of Anatomy and Cell Biology at the University of Toronto from 1991 until 2002, when he became a Professor in the Department of Medical Genetics and Microbiology. (wikipedia.org)
  • biochemical
  • Toxicity of organotin compounds: shared and unshared biochemical targets and mechanisms in animal cells. (semanticscholar.org)
  • In contrast to biochemistry, which involves to study of the chemistry of biomolecules and regulation of biochemical pathways within and between cells, chemical biology deals with chemistry applied to biology. (wikipedia.org)
  • bone-marrow cell
  • A megakaryocyte (mega- + karyo- + -cyte, "large-nucleus cell") is a large bone marrow cell with a lobulated nucleus responsible for the production of blood thrombocytes (platelets), which are necessary for normal blood clotting. (wikipedia.org)
  • melanocytes
  • For example, after 14 days, 20% to 40% of the cells in the melanocyte media took on a weblike shape typical of melanocytes. (webwire.com)
  • regeneration
  • Olfactory Stem Cells and Neural Regeneration. (berkeley.edu)
  • The regenerative capacity of the olfactory epithelium represents a powerful and experimentally accessible paradigm for understanding the regulation of neural stem cell function under normal conditions and during injury-induced regeneration. (berkeley.edu)
  • Reversal of type 1 diabetes via islet β cell regeneration following immune modulation by cord blood-derived multipotent stem cells. (docplayer.net)
  • Endogenous regeneration in the brain is the ability of cells to engage in the repair and regeneration process. (wikipedia.org)
  • While the brain has a limited capacity for regeneration, endogenous neural stem cells, as well as numerous pro-regenerative molecules, can participate in replacing and repairing damaged or diseased neurons and glial cells. (wikipedia.org)
  • amniotic fluid
  • A.-R. Prusa, E. Marton, M. Rosner, G. Bernaschek, and M. Hengstschläger, "Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research? (hindawi.com)
  • Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential," Cell Research , vol. 16, no. 4, pp. 329-336, 2006. (hindawi.com)
  • Pluripotency can be rapidly and efficiently induced in human amniotic fluid-derived cells," Human Molecular Genetics , vol. 18, no. 22, pp. 4340-4349, 2009. (hindawi.com)
  • Direct reprogramming of human amniotic fluid stem cells by OCT4 and application in repairing of cerebral ischemia damage," International Journal of Biological Sciences , vol. 12, no. 5, pp. 558-568, 2016. (hindawi.com)
  • Baculovirus-transduced mouse amniotic fluid-derived stem cells maintain differentiation potential," Annals of Hematology , vol. 88, no. 6, pp. 565-572, 2009. (hindawi.com)
  • Ferutinin promotes proliferation and osteoblastic differentiation in human amniotic fluid and dental pulp stem cells," Life Sciences , vol. 92, no. 20-21, pp. 993-1003, 2013. (hindawi.com)
  • pluripotent cells
  • The ability to progress via a definitive ectoderm intermediate will underpin the development of rational and efficient methodologies for the derivation of specific ectodermal cell populations from pluripotent cells in culture for use as experimental models or to produce cells with therapeutic applications. (biologists.org)
  • fetal
  • Single-cell genome analyses reveal the amount of mutations a human brain cell will collect from its fetal beginnings until death. (the-scientist.com)
  • Using the Golgi method, Giuseppe Magini then studied the mammalian fetal cerebral cortex in 1888, confirming the similar presence of elongated radial cells in the cortex (also described by Kölliker just before him), and observing "various varicosities or swellings" on the radial fibers. (wikipedia.org)
  • fates
  • Most research on this topic involves observing the assortment of chromosomal template strands through many cell development cycles and correlating non-random assortment with particular cell fates. (wikipedia.org)
  • transplantation
  • This process indicates that, subsequent to bone marrow transplantation, symmetrical cell divisions into two daughter HSCs must occur. (wikipedia.org)
  • In 2016, Keller co-founded Toronto-based BlueRock Therapeutics with Lorenz Studer for the development of iPSC-derived cells for clinical transplantation. (wikipedia.org)
  • isolate
  • Strand-seq (single strand sequencing) was first described in 2012 as a technique to isolate sequenced reads from parental template strands in single-cell DNA libraries. (wikipedia.org)
  • Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. (wikipedia.org)
  • neuronal
  • This feature makes the olfactory system particularly amenable for studies on adult neurogenesis and the properties of neuronal stem cells. (berkeley.edu)
  • A major goal of neuroscience is to understand how circuits of neurons and non-neuronal cells process sensory information, generate movement, and subserve memory, emotion and cognition. (berkeley.edu)
  • However, another in vivo study, using NSCs in the postnatal SVZ, showed that the stem cell is restricted to developing into different neuronal sub-type cells in the olfactory bulb. (wikipedia.org)
  • neurogenesis
  • Current projects are using a variety of approaches - including targeted gene knockouts to assess the function of transcription factors and stem cell factors, and single cell RNA sequencing - to elucidate the molecular and cellular mechanisms regulating olfactory stem cells and olfactory neurogenesis in the mouse. (berkeley.edu)
  • However, although there is evidence that both new neurons and glia are generated in the hippocampus in vivo, no exact relationship of neurogenesis to type 1 cells is shown. (wikipedia.org)
  • immune
  • The non-dilutive funding will support Gamida Cell's ongoing research and development efforts including its Phase III registration study of NiCord ® for hematological malignancies (blood cancers like leukemia and lymphoma), and its clinical trials of CordIn ® for sickle cell disease and thalassemia and NK cells as a potential immune therapy for cancer. (hematopoiesisnews.com)
  • Inflammation is a strong trigger of cell plasticity, particularly in recruited immune cells such as T cells and myeloid cells. (georg-speyer-haus.de)
  • It is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. (wikipedia.org)
  • The innate immune system, also known as the non-specific immune system or in-born immunity system, is an important subsystem of the overall immune system that comprises the cells and mechanisms that defend the host from infection by other organisms. (wikipedia.org)
  • The cells of the innate system recognize and respond to pathogens in a generic way, but, unlike the adaptive immune system, the system does not provide long-lasting immunity to the host. (wikipedia.org)
  • Cytokines produced by macrophages and other cells of the innate immune system mediate the inflammatory response. (wikipedia.org)
  • The complement system is a biochemical cascade of the immune system that helps, or "complements", the ability of antibodies to clear pathogens or mark them for destruction by other cells. (wikipedia.org)
  • White blood cells (WBCs), also called leukocytes or leucocytes, are the cells of the immune system that are involved in protecting the body against both infectious disease and foreign invaders. (wikipedia.org)
  • Lymphocytes leave the lymph node, as effector immune cells, via the efferent lymph vessels. (wikipedia.org)
  • This means immune cells may leave the lymph node along a chemokine gradient. (wikipedia.org)
  • definitive
  • The ability to form, recognize and manipulate the differentiation of definitive ectoderm is crucial to understanding the determination of cell fate in the ectoderm. (biologists.org)
  • and predicts formation of a cell population equivalent to the definitive ectoderm. (biologists.org)
  • It has been suggested that this area, in particular the ventral wall of the dorsal aorta, is one of the primary origins of the definitive haematopoietic stem cell. (wikipedia.org)
  • Definitive haematopoiesis is the second wave of embryonic haematopoiesis and give rise to all hematopoietic stem cells in the adult hematopoietic system. (wikipedia.org)
  • At 10 days post coitus (d.p.c.) the aorta gonad mesonephros region was able to initiate and expand definitive haematopoietic stem cell activity, whereas no haematopoietic activity was seen in the yolk sac until 11 d.p.c. (wikipedia.org)
  • renew
  • These cells are not able to renew their lysosomes (used in digesting microbes) and die after having phagocytosed a few pathogens. (wikipedia.org)
  • Inflammation
  • At the onset of an infection, burn, or other injuries, these cells undergo activation (one of their PRRs recognizes a PAMP) and release inflammatory mediators responsible for the clinical signs of inflammation. (wikipedia.org)
  • Neutrophils are the most common cell type seen in the early stages of acute inflammation. (wikipedia.org)
  • AGGF1 is critical to the specification of veins and multipotent hemanigioblasts, anti-inflammation, tumour angiogenesis, and inhibition of vascular permeability. (wikipedia.org)
  • induces
  • Thus, in X. laevis embryos, the ectoderm is patterned by the secretion of BMP antagonists from the dorsal blastopore lip, or Spemann's organizer: BMP signalling is active ventrally, and induces the formation of surface ectoderm, whereas dorsal BMP signalling is inhibited, and the cells adopt a neural fate ( Hemmati-Brivanlou and Melton, 1997 ). (biologists.org)
  • citation needed] In order to harvest stem cells from the circulating peripheral blood, blood donors are injected with a cytokine, such as granulocyte-colony stimulating factor (G-CSF), that induces cells to leave the bone marrow and circulate in the blood vessels. (wikipedia.org)
  • macrophages
  • These sinuses are cavities containing macrophages (specialised cells which help to keep the extracellular matrix in order). (wikipedia.org)
  • vivo
  • Recognition and analysis of these transient cell populations has been hampered by technical difficulties in accessing them in vivo. (biologists.org)
  • T cells deactivated by allogeniec umbilical cord blood stem cells ex vivo T cells infused back to patient to protect regenerating islet cells Patient monitored for changes in diabetes status. (docplayer.net)
  • ventricular
  • During the early development of a human, neural stem cells lie in the germinal layer of the developing brain, ventricular and subventricular zones. (wikipedia.org)
  • gene
  • Combining gene editing and stem-cell induction improves efficiency of functional genetic analyses. (the-scientist.com)
  • Promoter CpG methylation contributes to ES cell gene regulation in parallel with Oct4/Nanog, PcG complex, and histone H3 K4/K27 trimethylation," Cell Stem Cell , vol. 2, no. 2, pp. 160-169, 2008. (hindawi.com)
  • Macrophage stimulating 1 receptor (c-met-related tyrosine kinase), encoded by the MST1R gene, is a cell-surface receptor that binds macrophage-stimulating protein (MSP). (clontech.com)