• markers
  • Cloned SP cells cultured in medium with fibroblast growth factor-2 lost ABCG2 and PAX6 expression and upregulated several molecular markers of keratocytes, including keratocan, aldehyde dehydrogenase 3A1, and keratan sulfate. (mendeley.com)
  • Cloned corneal SP cells under chondrogenic conditions produced matrix staining with toluidine blue and expressed cartilage-specific markers: collagen II, cartilage oligomatrix protein, and aggrecan. (mendeley.com)
  • These results demonstrate the presence of a population of cells in the human corneal stroma expressing stem cell markers and exhibiting multipotent differentiation potential. (mendeley.com)
  • cell-permeable molecule called Cardiogenol C. We founded that Cardiogenol C could induce HBPCs to express transcription factors GATA4 Nkx2.5 and Tbx5 which are early specific markers for pre-cardiomyogenic cells. (elevateblogconference.com)
  • During this differentiation process, keratinocytes permanently withdraw from the cell cycle, initiate expression of epidermal differentiation markers, and move suprabasally as they become part of the stratum spinosum, stratum granulosum and eventually become corneocytes in the stratum corneum. (wikipedia.org)
  • Stem cells can also be isolated by their possession of a distinctive set of cell surface markers. (wikipedia.org)
  • germ
  • giving rise to any of the three germ layers of human (endoderm, ectoderm or mesoderm) as well as extra embryonic cells which include cytotrophoblast layer and a syncytiotrophoblast layer of the placenta . (medcraveonline.com)
  • The vasa gene, is essential for germ cell development and was first identified in Drosophila melanogaster, but has since been found to be conserved in a variety of vertebrates and invertebrates including humans. (wikipedia.org)
  • The Vasa protein is found primarily in germ cells in embryos and adults, where it is involved in germ cell determination and function, as well as in multipotent stem cells, where its exact function is unknown. (wikipedia.org)
  • In Drosophila, zygotic transcription of Vasa occurs at pole cells, and stays germ-line specific throughout the life of the organism. (wikipedia.org)
  • In P. hawaienis, Vasa transcript is uniformly distributed in the embryo and is localized depending on the stabilization of the 3'UTR (Untranslated Region) to the germ line cells. (wikipedia.org)
  • One of main function of Vasa protein is in germ cell determination and function. (wikipedia.org)
  • regenerative medicine
  • These stem cells may thus be a more practical source for regenerative medicine, particularly since, if placentas are routinely saved instead of thrown away, each individual will be able to draw on their own fetal stem cells if future therapeutic needs arise. (newswise.com)
  • embryo
  • A zygote is a totipotent stem cell which can even produce cells that are not actually the part of the embryo, example placental cells. (medcraveonline.com)
  • It has been seen in the developing embryo at stages E9.0 to E9.5 that there are a cluster of cells which give rise to the pancreas. (wikipedia.org)
  • Cells containing the desired DNA are incorporated into the host's embryo, resulting in a chimeric animal. (wikipedia.org)
  • lineage
  • Distinct characteristic of multipotent stem cells is they can only differentiate into cells of a particular lineage. (medcraveonline.com)
  • Molecular evidence for hierarchical transcriptional lineage priming in fetal and adult stem cells and multipotent progenitors. (ox.ac.uk)
  • Herein, global and single-cell analyses revealed a hierarchical organization of transcriptional lineage programs, with downregulation of megakaryocyte-erythroid genes from HSCs to LMPPs, sustained granulocyte-monocyte priming, and upregulation of common lymphoid (but not B and T cell-specific) genes. (ox.ac.uk)
  • Using in vivo lineage tracing techniques, Lgr5+ cells were found to contribute to the nephron, specifically to the ascending limb of the loop of Henle and the distal convoluted tubule. (wikipedia.org)
  • The megakaryocyte develops through the following lineage: CFU-Me (pluripotential hemopoietic stem cell or hemocytoblast) → megakaryoblast → promegakaryocyte → megakaryocyte. (wikipedia.org)
  • Genetic lineage tracing experiments have been performed by various research groups to show that the cell clusters originating from the developing foregut express a transcription factor called PDX1 (Pancreatic and duodenal homeobox 1). (wikipedia.org)
  • placental stem
  • Newswise - May 17, 2012 - Oakland, Calif. - Scientists at Children's Hospital Oakland Research Institute (CHORI) led by Vladimir Serikov, MD, PhD, and Frans Kuypers, PhD, report in the current Epub issue of Stem Cells Translational Medicine (1) that placental stem cells with important therapeutic properties can be harvested in large quantities from the fetal side of human term placentas (called the chorion). (newswise.com)
  • Since these functional placental stem cells can be isolated from either fresh or frozen term human placentas, this implies that if each individual's placenta is stored at birth instead of thrown away, these cells can be harvested in the future if therapeutic need arises. (newswise.com)
  • Placental stem cells are only 9 months old, and in contrast to adult stem cells, do not need to be reprogrammed to become pluripotent. (newswise.com)
  • Transcription
  • The Transcription factor Aire (autoimmune regulator) that controls the expression of PTAs on mTEC cells in the thymus is only expressed at low levels by uncharacterized double negative stromal cells. (wikipedia.org)
  • developmental
  • EB has the advantage of providing a three-dimensional structure, which enhances cell-cell interactions that may be important for some developmental processes. (hindawi.com)
  • These genes regulates the expression of Mnx1 in the ventral bud, leading to the developmental specification of the pancreatic progenitor cells expressing Pdx1. (wikipedia.org)
  • endoderm
  • It is important to note here that the developing dorsal and ventral buds are characterized as endoderm, and it isn't until the expression of Pdx1 (specification of endoderm to a stem cell multipotent state) that the endoderm to pancreatic progenitor transition occurs. (wikipedia.org)
  • potential to differentiate
  • JULY 12, 2006 - (Philadelphia, PA) - Researchers at the University of Pennsylvania School of Medicine have isolated a new source of adult stem cells that appear to have the potential to differentiate into several cell types. (webwire.com)
  • Ne w Rochelle, NY, May 2, 2017 -Researchers have shown that following a stroke-induced ischemic injury to the human brain, stem cells are produced that have the potential to differentiate and mature to form neurons that can help repair the damage to the brain. (liebertpub.com)
  • functional
  • The next challenge will be to demonstrate the functional utility of these cells, both in vitro and in preclinical models of bone and vascular diseases. (hindawi.com)
  • progenitors
  • The differential of pancreatic progenitors from hepatic progenitors is also notable, as Hhex1 (Hematopoietically expressed homeobox gene) is responsible for the origination of pancreatic progenitor cells. (wikipedia.org)
  • glial cells
  • In this capacity, glial cells provide multiple functions to both the central nervous system (CNS) and the peripheral nervous system (PNS). (wikipedia.org)
  • Understanding the mechanisms in which gliogenesis is regulated provides the potential to harness the ability to control the fate of glial cells and, consequently, the ability to reverse neurodegenerative diseases. (wikipedia.org)
  • Proneural factors are expressed in high concentrations during times in which glial cells are not to form or neuron development is needed. (wikipedia.org)
  • Independently, Stallcup and colleagues generated an antiserum that recognized a group of rat brain tumor cell lines, which exhibited properties that were intermediate between those of typical neurons and glial cells. (wikipedia.org)
  • progeny
  • The two fundamental characteristics of stem cells are the ability of unlimited self-regeneration and the production of at least one type of differentiated progeny cells . (medcraveonline.com)
  • Those stem cells and their differentiated progeny are organized into columns named epidermal proliferation units. (wikipedia.org)
  • therapies
  • Totipotent stem cells, however, out of all the other types, presents an ideal approach to deal with the therapies related to the diseases . (medcraveonline.com)
  • Previous studies have produced conflicting results, and some researchers have questioned whether stem cell therapies for failing. (bionews.org.uk)
  • The study's first author, Dr Vashe Chandrakanthan, who developed the technology, said the new technique is an advance on other stem cell therapies being investigated, which have a number of deficiencies. (srilankanscientist.com)
  • Adult stem cells are frequently used in various medical therapies (e.g., bone marrow transplantation). (wikipedia.org)
  • self-renewal
  • The continuous self-renewal and differentiating ability of stem cells has created modern research emphasis on it, especially in relation to its therapeutic use . (medcraveonline.com)
  • Proliferation capacity, self-renewal capacity, and ability for multipotent differentiation were analyzed by BrdU labeling, flow cytometry, reverse transcriptase-polymerase chain reaction, immunocytochemistry, and in vivo transplantation. (arvojournals.org)
  • The classical definition of a stem cell requires that it possesses two properties: Self-renewal: the ability to go through numerous cycles of cell division while maintaining the undifferentiated state. (wikipedia.org)
  • Unipotent cells can produce only one cell type, their own, but have the property of self-renewal, which distinguishes them from non-stem cells (e.g. progenitor cells, which cannot self-renew). (wikipedia.org)
  • cellular
  • Among them, Till is: University Professor Emeritus at the University of Toronto Editorial member of the open access journal Journal of Medical Internet Research Founding Board member of the Canadian Stem Cell Foundation McCulloch continued to expand the depth of work in his field with a heavy emphasis on cellular and molecular mechanisms affecting the growth of malignant blast stem cells from the blood of patients with acute myeloblastic leukemia. (wikipedia.org)
  • Finally, microglial cells are derived from glial precursors and carry-out macrophage like properties to remove cellular and foreign debris within the central nervous system ref. (wikipedia.org)
  • genes
  • There, beta-catenin interacts with various coactivators including CREB-binding protein (CBP) and p300, which results in the expression of different genes downstream of beta-catenin/TCF that may direct cells towards a path of pluripotency or differentiation. (usc.edu)
  • Planar cell polarity (PCP) genes have been identified in Drosophila to be important for establishing polarity in various processes, including hair follicle orientation. (usc.edu)
  • novel
  • In previous work, Drs. Serikov and Kuypers reported a novel technology to harvest blood-forming stem cells from the placenta to augment cord blood cells (2). (newswise.com)
  • Catherine Verfaillie and colleagues at the University of Minnesota first described these novel stem cells in 2002, but other teams have had difficulty in replicating the work. (bionews.org.uk)
  • Distinct
  • In the forebrain, three regionally distinct sources have been shown to generate polydendrocytes sequentially: an early ventral source from the medial ganglionic eminence marked by Nkx2.1, followed by progenitor cells in the lateral ganglionic eminence marked by Gsh2, and finally the dorsal neocortical germinal zone marked by Emx1. (wikipedia.org)
  • precursors
  • In one series of studies on the development and origin of oligodendrocytes in the rodent CNS, a population of immature cells that appeared to be precursors to oligodendrocytes was identified by the expression of the GD3 ganglioside. (wikipedia.org)
  • pancreas
  • The Adult Mouse and Human Pancreas Contain Rare Multipotent Stem Cells" by Simon R. Smukler, Margot E. Arntfield et al. (uwindsor.ca)
  • Based on his experiments with human cadaveric pancreata, van der Kooy holds theorizes that there exists an adult stem cell in the pancreas. (wikipedia.org)
  • The fused and developed pancreas consists of pancreatic enzyme secreting cells (exocrine cells), digestive enzyme transporting cells (ductal cells) and hormone producing cells (endocrine cells). (wikipedia.org)
  • These endocrine cells develop in discrete areas within the pancreas known as the islets. (wikipedia.org)
  • Notch signaling has been shown to regulate the number of exocrine and endocrine cells in the pancreas, but not without the presence of Pdx1. (wikipedia.org)
  • Proliferation
  • Based on the observation that these cells require PDGF for their proliferation and expansion, the expression of the alpha receptor for platelet-derived growth factor (Pdgfra) was used to search for the in vivo correlates of the A2B5+ cells, which led to the discovery of a unique population of Pdgfra+ cells in the CNS whose appearance and distribution were consistent with those of developing oligodendrocytes. (wikipedia.org)
  • After the committed progenitor cells exit the germinal zones, they begin to express NG2 and Pdgfra and expand by local proliferation and migration and eventually occupy the entire CNS parenchyma. (wikipedia.org)
  • BrdU
  • Using stain-retaining assay (with bromodeoxyuridine, or BrdU), a low-cycling cell population was found in the papillary region, which was able to divide rapidly to repair the damaged caused by transcient renal ischemia. (wikipedia.org)
  • vivo
  • Ex vivo NIR TD optical imaging results showed a systemic distribution of labelled cells. (duhnnae.com)
  • As noted for receptor-proteins, in vivo interactions among different growth factor responsible for gliogenesis and other cell fates produce very different roles than when isolated. (wikipedia.org)
  • epithelial
  • It has been reported that endogenous kidney tubular renal epithelial cells can be dedifferentiated into induced pluripotent stem cells by the treatment of only two factors - Oct4 and Sox2. (wikipedia.org)
  • Dr. Gordon's laboratory has investigated epithelial cell interaction with human-associated pathogens, including uropathogenic Escherichia coli, Helicobacter pylori, and Listeria monocytogenes. (wikipedia.org)
  • Syndecan-1 expression in prostate cancer and the role of p27 in primary prostate epithelial cell transformation. (wikipedia.org)
  • Identification
  • These findings have lead to identification of several pathways controlling the differentiation of hESCs into mesodermal derivatives such as myoblasts, mesenchymal cells, osteoblasts, chondrocytes, adipocytes, as well as hemangioblastic derivatives. (hindawi.com)
  • abcg2
  • The ABCG2-expressing cell population was isolated as a side population (SP) by cell sorting after exposure to Hoechst 33342 dye. (mendeley.com)
  • The SP cells exhibited clonal growth and continued to express ABCG2 and also PAX6, product of a homeobox gene not expressed in adult keratocytes. (mendeley.com)
  • Department of Anatomy
  • In 1981, he became an Assistant Professor, was promoted to Associate Professor in 1986, and has served as Professor in the Department of Anatomy and Cell Biology at the University of Toronto from 1991 until 2002, when he became a Professor in the Department of Medical Genetics and Microbiology. (wikipedia.org)