• Mucin-1 (MUC-1) is a heterodimer transmembrane protein of the mucin family encoded in humans by the MUC1 gene. (wikipedia.org)
  • Mucin-1 kidney disease, previously described as medullary cystic kidney disease type 1 (MCKD1, OMIM 174000), is an autosomal dominant tubulointerstitial kidney disease recently shown to be caused by a single-base insertion within the variable number tandem repeat region of the MUC1 gene. (mit.edu)
  • The Role of the Cell Surface Mucin MUC1 as a Barrier to Infection and Regulator of Inflammation.Front Cell Infect Microbiol 24;9:117. (immuquest.com)
  • Membrane bound mucins MUC1 and MUC3 appear to be associated with the development of gastric carcinoma. (bmj.com)
  • Aberrant (upregulated) expression of the MUC1 mucin has been seen in breast carcinomas and other neoplasms, such as colon and pancreatic cancers. (bmj.com)
  • 1, 2 Most studies of MUC1 expression in breast carcinomas have shown that increased membrane MUC1 mucin expression on the apical cell surface is associated with a better prognosis (reviewed in Rahn and colleagues 2 ), whereas circumferential staining in tumour cell cytoplasm is associated with a worse prognosis. (bmj.com)
  • Krebs von den Lungen-6 (KL-6) is a human mucin 1 (MUC1) glycoprotein, which has shown diagnostic and prognostic value as a biomarker in other malignancies. (lu.se)
  • Plasma Mucin-1 (CA15-3) Levels in Autosomal Dominant Tubulointerstitial Kidney Disease due to MUC1 Mutations. (cdc.gov)
  • This disease, previously referred to as MCKD type 1, is due to a mutation in the variable-number tandem repeat region of the MUC1 (Mucin 1) gene. (medscape.com)
  • Cancer antigen 27-29 (or CA 27-29) is an epitope of a large transmembrane mucin glycoprotein named MUC1 that is expressed on the cell surfaces of most glandular epithelia. (medscape.com)
  • The MUC7 gene belongs to a group of genes encoding soluble mucin glycoproteins. (benthamscience.com)
  • Mucus comprises water, glycoproteins (mucins), and ions. (bmj.com)
  • However, it is critical to note that oligosaccharides containing a fucose α(1-3)-linked to the glycan core often found in plant and insect glycoproteins are resistant to PNGase F, and would therefore require PNGase A treatment. (neb.com)
  • Mammalian mucins, glycoproteins that are major constituents of the glycocalyx that covers mucosal epithelium. (embl.de)
  • Sheet mask dipped in 35,000 ppm of snail mucin delivers nourishment and hydration skin deep! (kiokii.com)
  • P - selectin glycoprotein ligand - 1 (PSGL - 1) is a mucin - type protein that is heavily substituted with O - glycans. (avhandlingar.se)
  • also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). (rndsystems.com)
  • A glycoprotein that forms mucin. (wordinfo.info)
  • CA 27-29 represents a sequence of mucins on the extracellular region of this glycoprotein. (medscape.com)
  • Alterations to the cytoplasmic tail may affect movement through the Golgi apparatus, thus affecting glycosylation of the tandem repeat domains of MUC-1. (wikipedia.org)
  • The C-terminus of MUC-1 (MUC-1 C) is short-the majority of weight comes from N-glycosylation. (wikipedia.org)
  • O-glycosylation and N-glycosylation in MUC-1 contribute to the formation of mucin. (wikipedia.org)
  • MUC-1 also exhibits altered glycosylation and aberrant surface distribution patterns in tumor cells. (wikipedia.org)
  • The mucin - type protein was used as a probe to analyze the O - glycosylation capacity of the se cell lines, which today are used for the commercial production of recombinant proteins and vaccine co mponents. (avhandlingar.se)
  • The glycosylation of PSGL - 1/mIgG 2b may be tailored by producing the protein in genetically engineered cell lines. (avhandlingar.se)
  • Rational glycan design is achieved by transfecting cells with plasmids encoding PSGL - 1/mIgG 2b together with specific glycosyltransferases that expand the glycosylation capacity of the cells. (avhandlingar.se)
  • The mucin region undergoes extensive O-linked glycosylation. (rndsystems.com)
  • Here, we focus on the salivary mucin-7 gene ( MUC7 ) to study the evolution and functional impact of subexonic repeats. (nature.com)
  • These repeats can be particularly well studied in this gene because the repeats are relatively short and few in number compared to other mucins. (nature.com)
  • The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). (rndsystems.com)
  • In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice. (taconic.com)
  • Co-author Professor Adrian Woolf from Manchester Children's Hospital and The Universality of Manchester said: "One of the genes -- mucin-1- is especially interesting. (sciencedaily.com)
  • The present review focuses on circulating miRNAs in patients with TNBC, with an emphasis on their interaction with the immune response checkpoint genes PD‑1, PD‑L1 and CTLA4. (spandidos-publications.com)
  • Kim YD, Cho JS, Kim HS, Song SH: Effect of macrolide on the interleukin-1 β-mediated MUC2/5AC genes expression and mucin secretion in human airway epithelial cells. (karger.com)
  • Furthermore, the enhanced airway mucins in the MyD88- deficient mice were not due to defective secretion, as the mucin secretory capacity of MyD88-KO mice remained intact. (cdc.gov)
  • To investigate this matter further we have used an approach employing combinatorial antibody libraries and antigen-specific selection in the search for CDRH3 length and sequence diversity compatible with a given antigen specificity, the major antigenic determinant on the tumour-associated antigen mucin-1. (nih.gov)
  • Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) has been recently identified as a heterophilic ligand for Tim-3. (medscimonit.com)
  • [ 1 ] This protein, also known as polymorphic epithelial mucin or epithelial membrane antigen, has a large extracellular region, a transmembrane sequence, and a cytosolic domain. (medscape.com)
  • Tumor related MUC-1 disrupts and inhibits cell-cell and cell-matrix interactions and adherence. (wikipedia.org)
  • Aug. 1, 2022 Kidney stones can cause not only excruciating pain but also are associated with chronic kidney disease, osteoporosis and cardiovascular disease. (sciencedaily.com)
  • The multivalent mucins could adsorb chain type - sp ecific anti - A antibodies, which indicate a prospective use of the mucins in immunoadsorption (IA) columns. (avhandlingar.se)
  • Using CDR shuffling, residues important for the assembly of mucin-1 specific paratopes were defined by random recombination of the complementarity determining regions derived from a set of mucin-1 specific clones, previously selected from an antibody fragment library. (lu.se)
  • MUC7 provides an opportunity to assess the broader implications of subexonic repeat variation within the context of the functional evolution of the mucin functional group and primate salivary adaptation. (nature.com)
  • Mucin MUC7, Mucins - Potential Regulators of Cell Functions Volume Title: Gel-Forming and Soluble Mucins (2013) 1: 398. (benthamscience.com)
  • Epithelial mucins can be classified into two distinct families: secretory (gel forming) and membrane bound. (bmj.com)
  • MUC-1 in cancerous epithelial cells exhibits a loss of polarity. (wikipedia.org)
  • To evaluate the effect of multipurpose contact lens care solutions (MPSs) on membrane-associated mucins (Muc 1 & 16) expressions in the cornea using SV40 transformed Human corneal epithelial Cells (HCET) and Rat cornea tissue sections. (arvojournals.org)
  • The effects of serum on mucin (MUC-1) expression were observed in cultured conjunctival epithelial cells in vitro. (bmj.com)
  • The additive effect of human serum for cultured conjunctival epithelial cells showed significant MUC-1 upregulation on the cell surface. (bmj.com)
  • The present study demonstrates that AZM and MDM suppress the synthesis of mucin and TNF-α from human airway epithelial cells. (karger.com)
  • 1, 2 This review approaches the role of mucin degrading enzymes from the potential interaction of micro-organisms with the cervical mucus barrier, and discusses the contribution that mucin degrading enzymes may make to microbial colonisation of the reproductive tract. (bmj.com)
  • Because of variable age of disease onset and often subtle signs and symptoms, clinical diagnosis of mucin-1 kidney disease and differentiation from other forms of hereditary kidney disease have been difficult. (mit.edu)
  • We therefore developed and validated a mass spectrometry-based probe extension assay with a series of internal controls to detect the insertion event using 24 previously characterized positive samples from patients with mucin-1 kidney disease and 24 control samples known to be wild type for the variant. (mit.edu)
  • Validation results indicate an accurate and reliable test for clinically establishing the molecular diagnosis of mucin-1 kidney disease with 100% sensitivity and specificity across 275 tests called. (mit.edu)
  • MUC-1 in cancer is underglycosylated, causing interactions to form between MUC-1 core protein, transmembrane receptors, and extracellular components. (wikipedia.org)
  • Almasmoum H. The Roles of Transmembrane Mucins Located on Chromosome 7q22.1 in Colorectal Cancer.Cancer Manag Res. (immuquest.com)
  • Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). (rndsystems.com)
  • namely, MUC3, MUC4, MUC12, MUC13, and the recently described mucin MUC17, which share a C-terminal structural domain that consists of two or three epithelial growth factor (EGF)-like regions, a transmembrane segment, and a short cytoplasmic tail. (bmj.com)
  • EBV-associated gastric cancer evades T-cell immunity by PD-1/PD-L1 interactions. (researchmap.jp)
  • MUC-1 protein is estimated to weigh 120 to 225 kDA. (wikipedia.org)
  • The extracellular sperm protein-enterokinase-agarin (SEA) domain of MUC-1 contributes to a variety of functions including: inhibition of immune response, resistance to stimuli, and regulation of cell shedding. (wikipedia.org)
  • After the incubation period, the cells are removed and secreted protein 'spots' are detected by immunological techniques and counted, where each spot corresponds to 1 live CTC (Figure 4). (medscape.com)
  • CA 15-3 which detects soluble forms of MUC-1 protein is the most widely used serum marker in patients with breast cancer. (nih.gov)
  • PSGL - 1 genetically fused to mouse IgG 2b Fc forms a dimeric PSGL - 1/mIgG 2b mucin - type fusion protein. (avhandlingar.se)
  • It makes a sticky protein called mucin that coats urinary tubes inside the kidney. (sciencedaily.com)
  • Dendritic cells transfected with heat-shock protein 70 messenger RNA for patients with hepatitis C virus-related hepatocellular carcinoma: a phase 1 dose escalation clinical trial. (researchmap.jp)
  • T cell immunoglobulin and mucin domain 3 (Tim-3) is a key checkpoint receptor responsible for exhaustion and dysfunction of T cells and plays a critical role in immunosuppression. (medscimonit.com)
  • Alterations to these areas have shown a propensity of metastasis and progression in comparison to unaltered MUC-1 domains. (wikipedia.org)
  • In general, mucins have the unique function of protecting and lubricating epithelial surfaces, but in recent years they have also been implicated in additional diverse roles, such as growth, fetal development, epithelial renewal and differentiation, epithelial integrity, carcinogenesis, and metastasis. (bmj.com)
  • Mucin is a mucous-like substance that is produced by cells in the conjunctiva and outer surface of the eye. (1800getlens.com)
  • Mucinases are enzymes capable of degrading mucins (the complex high molecular weight molecules that are the major non-aqueous components of mucous gels). (bmj.com)
  • MUC-1 regulates tyrosine kinase signaling receptors, which promote synthesis of biosynthetic intermediates used in cell growth. (wikipedia.org)
  • Mucins are highly glycosylated proteins that serve a variety of roles in organisms from lubrication to cell-signaling to defense 7 . (nature.com)
  • IWR-1-endo potently inhibits WNT signaling by blocking a cell-based WNT/β-catenin pathway reporter response with an IC₅₀ value of 180 nM (Chen et al. (stemcell.com)
  • The extracellular domain of MUC-1 is composed of 20 identical amino acid tandem repeats (TR). (wikipedia.org)
  • MUC-1 is an inhibitor for cell-to-cell extracellular interactions for both normal and malignant cells. (wikipedia.org)
  • In paper II, genetically engineered Chinese Hamster Ovary (CHO) cells were used to produce PSGL - 1/mIgG 2b carrying blood group A and B determinants on type 1, 2 and 3 outer core saccharide chains. (avhandlingar.se)
  • 1-3 Rose bengal staining, fluorescein staining, impression cytology, and brush cytology show greater changes in SS dry eye owing to a lack of both basic tearing and reflex tearing resulting from lacrimal gland destruction by infiltrating lymphocytes, which is the hallmark of deteriorating clinical conditions. (bmj.com)
  • Several studies have reported conflicting and inconclusive results concerning the clinical relevance of mucin expression in gastric carcinoma. (bmj.com)
  • Clinical transplantation 2020 1 34 (2): e13783. (cdc.gov)
  • Given, MUC-1 is 10 times higher in cancer cells than normal cells, an over expression of MUC-1 in cancer can be indicative of aggressive, metastatic cancer, having a low response to therapy and survival rate. (wikipedia.org)
  • In cancer cells, over expression of MUC-1 is seen throughout the cell's nucleus, plasma membrane and cytoplasm. (wikipedia.org)
  • MUC-1 over expression is controlled through transcription changes, post-translational, and amplification modifications. (wikipedia.org)
  • Expression of mucin 1 possessing a 3'-sulfated core1 in recurrent and metastatic breast cancer. (researchmap.jp)
  • This study investigated the correlations between aberrant expression of mucins in gastric carcinoma and patient clinicopathological features. (bmj.com)
  • Capable of bringing about the hydrolysis (splitting of a compound into fragments by the addition of water) of mucin, as by a mucinase (an enzyme that acts upon mucin). (wordinfo.info)
  • Membrane-associated mucins are one of the major components of the ocular surface that play a vital role in the maintenance of the ocular surface integrity. (arvojournals.org)
  • 1-5 Accumulated knowledge in the past decade has demonstrated the importance of tear components such as epidermal growth factor (EGF) and vitamin A in the regulation of proliferation, differentiation, and maturation of the ocular surface epithelium. (bmj.com)
  • Partial or complete degradation of mucin molecules by microbial enzymes is often a fundamental step in disruption of defensive mucosal barriers, as these constitute direct interfaces between internal and external environments. (bmj.com)
  • In normal cells, MUC-1 is isolated to the apical surface of the cell. (wikipedia.org)
  • Mucin 1 lies on the tubular cell apical surface and has a role in signal transduction pathways. (medscape.com)
  • MUC-1 is located in the apical membrane on simple epithelial cell surfaces. (wikipedia.org)
  • MUC-1 is removed from the membrane by endocytosis, internalized, re-glycosylated and recycled to the cell membrane. (wikipedia.org)
  • MUC-1 provides protection to the apical membrane to prevent rupture, as well as environmental and immune attack. (wikipedia.org)
  • Membrane-associated mucins (Muc 1 and Muc 16) expressions were subsequently analyzed by Western blot. (arvojournals.org)
  • Cornea sections and lysates were prepared from the eyes of the treated rats and used for the immunohistochemistry and Western blot analysis of membrane-associated mucins expressions. (arvojournals.org)
  • Western blot was also used to analyze the effect of 0.1% macrogolglycerol hydroxystearate (HCO), 0.1% poloxamer, 0.1% poloxamine, 5 pm polyaminopropyl biguanide (PHMB), 0.05% boric acid, and 0.1% boric acid, common MPSs ingredients, on membrane-associated mucins (Muc 1 and Muc 16 ) expressions in HCET after 24 hours treatment. (arvojournals.org)
  • Western blot results showed that MPSs containing boric acid down-regulate membrane-associated mucins in the cornea while MPSs without boric acid had no effect on membrane-associated mucins. (arvojournals.org)
  • Immunohistochemistry analysis of membrane-associated mucins expressions confirmed the results of the Western blot analysis. (arvojournals.org)
  • MUC-1 is a transcriptional coactivator involved in the activity and stabilization of enzymes and transcription of metabolic functions. (wikipedia.org)
  • The possible contribution of mucin degrading enzymes to the pathogenesis of infection is, therefore, not to be underestimated. (bmj.com)
  • The peptide backbone exhibits glycosidic bonds between serine and threonine within MUC-1 N. Within the cytoplasmic tail of MUC-1, multiple phosphorylation sites exist due to the presence of threonine, tyrosine and serine amino acid residues. (wikipedia.org)
  • 1. Presence of, or accumulation of, mucin in the blood. (wordinfo.info)
  • 2. The presence of mucin in blood, a condition that may occur in metastatic malignancies of the gastrointestinal tract or the ovaries. (wordinfo.info)
  • Presence of mucin in the urine, suggesting vaginal contamination. (wordinfo.info)
  • MUC-1 has been shown to repair epithelia through the activation of epigenetic reprogramming, epithelial-mesenchymal transition and self-renewing (stemness) in maintaining epithelial cell homeostasis. (wikipedia.org)
  • The intercellular interaction between MUC-1 and the receptor ICAM-1 facilitates endothelial and epithelial cell interactions, allowing circulating cancer cells to adhere in the inner lining of blood vessels and thus migrate. (wikipedia.org)
  • In paper I, PSGL - 1/mIgG 2b was produced in Sf9 ( S podoptera frugiperda ) and Hi - 5 ( Trichoplusia ni ) cell lines. (avhandlingar.se)
  • It remains to be shown if PSGL - 1/mIgG 2b can competitively inhibit and sterically block toxin and viral attachment to the cell surface. (avhandlingar.se)
  • TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). (rndsystems.com)
  • 1). A common post-translational modification essential for cell viability is the attachment of glycans, as shown in Figure 1. (neb.com)
  • In normal cells, the tandem repeats and cytoplasmic tail of MUC-1 are significant in the regulation and progression of metastatic cancer. (wikipedia.org)
  • The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. (rndsystems.com)
  • The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). (rndsystems.com)
  • Total mucin in culture supernatants was measured using enzyme-linked lectin assay. (karger.com)
  • Studies vary in their estimations of this percentage, but at least one survey suggests that approximately 20 percent of people in the United States suffer from dry eye syndrome.1 Some researchers believe 75 percent of the population over the age of 65 experiences dry eye symptoms. (1800getlens.com)
  • Detects human TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. (rndsystems.com)
  • The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. (rndsystems.com)
  • With the use of an established model, C57BL/6J wild-type (WT) and global MyD88 knockout (KO) mice were treated with intranasal inhalation of ODE or saline, daily for 1 wk. (cdc.gov)
  • Missense mutations in UMOD cause tubulointerstitial nephropathy with hyperuricemia , previously named MCKD type 2 or juvenile hyperuricemic nephropathy type 1. (medscape.com)
  • 1) takes omohyoid, submandibular gland, sensory nerves C2-C5, cervical branch of facial nerve, and ipsilateral thyroid2) same as above + accessory nerve (CN XII), SCM, and internal jugular resection. (brainscape.com)
  • BMC nephrology 2018 10 19 (1): 301. (cdc.gov)
  • Multipurpose solutions' composition should be clinically controlled because Mucins play a very important role in the ocular surface integrity maintenance and the tear film stability. (arvojournals.org)
  • no rx.2) same as 1 but on lingual surface of mandible. (brainscape.com)
  • Thiago S. Carvalho Casein and mucin have been shown to improve the erosion -protective properties of the pellicle when applied in combination. (karger.com)