Minichromosome maintenance proteins. Medical search. Frequent questions

Proteins were enzymatically digested into peptides which were measured by high-resolution mass spectrometry and analyzed by "all-or-nothing" analysis, Bonferroni, and Benjamini-Hochberg correction for multiple testing. (oncotarget.com)

A minichromosome is a small chromatin-like structure resembling a chromosome and consisting of centromeres, telomeres and replication origins but little additional genetic material. (wikipedia.org)
Next, the desired contents of the minichromosome must be transformed into a host which is capable of assembling the components (typically yeast or mammalian cells) into a functional chromosome. (wikipedia.org)
Unlike traditional methods of genetic engineering, minichromosomes can be used to transfer and express multiple sets of genes onto one engineered chromosome package. (wikipedia.org)
Minichromosome technology allows for the stacking of genes side-by-side on the same chromosome thus reducing likelihood of segregation of novel traits. (wikipedia.org)
mini chromosome maintenance. (nih.gov)
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. (bionity.com)
The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. (thermofisher.com)
MORF4-related gene-binding protein (MRGBP), which is also known as chromosome 20 open reading frame 20 (C20orf20), is commonly highly expressed in several types of malignant tumors and tumor progression. (oncotarget.com)
In a very last part of this work the mini-chromosome maintenance com-plex was investigated using FCS and cryo-TEM. (uni-bayreuth.de)
Accurate chromosome segregation during mitosis requires the assembly of centromeric DNA and proteins to form the kinetochore, which couples chromosome movement to dynamic spindle microtubules. (rupress.org)
Targeted exome sequencing identified a variant (c.71-1insG) in mini chromosome maintenance homologue 4 (MCM4) that segregated with the disease in all 3 families. (qmul.ac.uk)

A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. (bionity.com)
Targeted exome sequencing in 8 patients identified a variant (c.71-1insG) in minichromosome maintenance-deficient 4 (MCM4) that was predicted to result in a severely truncated protein (p.Pro24ArgfsX4). (jci.org)
From a large number of ORF59 interacting proteins, we pursued few candidates for further study including host proteins Nucleolin and MCM4 in addition to viral protein, ORF57. (unr.edu)
Western blotting of patient lymphocytes revealed loss of the full length MCM4 protein but two smaller MCM4 isoforms were preserved. (qmul.ac.uk)

The MCM complex consisting of this protein and MCM2, 4 and 7 possesses DNA helicase activity, and may act as a DNA unwinding enzyme . (bionity.com)
At the first stage of the cell division cycle, termed G1, minichromosome maintenance proteins Mcm2-7 are loaded onto the DNA in preparation for the next stage (termed S-phase), when DNA is replicated (copied). (dundee.ac.uk)
In cancer cells, this checkpoint is lost so that cells can progression into S-phase with insufficient Mcm2-7 proteins attached to the DNA, meaning that DNA replication cannot be completed and the cancer cell cannot divide again. (dundee.ac.uk)
c-ErbB1/EGFR), HER-2/neu (c-ErbB2/HER-2), Ki-67, and minichromosome maintenance protein 2 (MCM2) expression in bronchial dysplasia was undertaken to characterize molecular alterations associated with the progression of these lesions in 268 bronchoscopically obtained biopsies from 134 subjects. (nih.gov)
This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. (utsouthwestern.edu)
We show in this manuscript, using purified proteins from budding yeast, that Mcm10 directly interacts with the Mcm2-7 complex and Cdc45. (fsu.edu)
The hexameric protein complex formed by MCM proteins (MCM2-7) plays an essential role in the initiation and regulation of DNA replication during the cell cycle. (biogenex.com)

Chromosomal Proteins, Non-Histone" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
Below are the most recent publications written about "Chromosomal Proteins, Non-Histone" by people in Profiles. (umassmed.edu)

1996). "Binding of human minichromosome maintenance proteins with histone H3. (bionity.com)
Binding to co-regulatory proteins, such as chromatin remodeling and histone modifying enzymes, occurs through one or more transactivation domains (TADs, also called activation domains) present in TFs ( 1 , 2 ). (frontiersin.org)
Here, we report that JMJD5, a Jumonji C (JmjC) domain-containing protein, is a Cathepsin L-type protease that mediates histone H3 N-tail proteolytic cleavage under stress conditions that cause a DNA damage response. (cusabio.com)

The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. (bionity.com)
A hexameric protein complex of minichromosome maintenance proteins. (bvsalud.org)
MCM3, minichromosome maintenance protein 3 is a part of the hexameric MCM DNA helicase, belongs to the MCM family. (biogenex.com)

Firstly I investigated 2 families of Turkish ethnicity who presented with delayed onset adrenal insufficiency and identified two novel missense mutations in the melanocortin 2 receptor accessory protein (MRAP). (qmul.ac.uk)

MCM6 , or minichromosome maintenance deficient 6 gene is one of the highly conserved MCMs that are essential for the initiation of eukaryotic genome replication. (bionity.com)
MCM6 produces, by alternative splicing, 3 different transcripts, all with introns, putatively encoding 3 different protein isoforms. (bionity.com)
Entrez Gene: MCM6 minichromosome maintenance deficient 6 homolog (S. cerevisiae). (bionity.com)
1998). "Human protein MCM6 on HeLa cell chromatin. (bionity.com)

Mediator, a large modular protein complex with varying subunit composition, bridges TFs with Pol II and coordinates DNA-loop formation, transcriptional initiation, and post-initiation events ( 3 , 4 ). (frontiersin.org)
The protein complex MCM (yellow) limits the loop formation. (mpg.de)
Now Kikuë Tachibana, new director of the department "Totipotency" at the Max Planck Institute of Biochemistry, and her team discovered that a protein complex well known for its function in DNA replication has an unexpected role in genome folding. (mpg.de)
In this current study, they have now jointly identified the minichromosome maintenance (MCM) complex as a new class of barriers in the formation of DNA loops. (mpg.de)
In the process of DNA loop formation, also called loop extrusion, three proteins or protein complexes are mainly involved: first cohesin, second the zinc finger protein CTCF, and third the MCM complex. (mpg.de)
a protein that binds to the Cdt1 component of the pre-replication complex and inactivates it, preventing assembly of the pre-replication complex at inappropriate times during the cell cycle. (nih.gov)
They form into a protein complex that has helicase activity and is involved in a variety of DNA -related functions including replication elongation, RNA transcription, chromatin remodeling, and genome stability. (bvsalud.org)
After much trial and error, the team ultimately tried insect cells for expression of this human protein, which finally resulted in stable pure protein that allowed cryo-EM imaging of the MCM8/9 complex. (ecancer.org)
Minichromosome Maintenance Complex Component 4" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
A minichromosome maintenance protein that is a key component of the six member MCM protein complex. (uchicago.edu)
This graph shows the total number of publications written about "Minichromosome Maintenance Complex Component 4" by people in this website by year, and whether "Minichromosome Maintenance Complex Component 4" was a major or minor topic of these publications. (uchicago.edu)
Below are the most recent publications written about "Minichromosome Maintenance Complex Component 4" by people in Profiles. (uchicago.edu)
ORF59 also associated with minichromosome maintenance proteins (MCMs), a complex of DNA replication licensing factor required for cellular DNA replication. (unr.edu)
Understanding their complex cell architecture and life cycle is challenging because, as with most eukaryotic microbes, ~50% of genome-encoded proteins have completely unknown functions. (nature.com)

Minichromosome maintenance protein 10 (Mcm10) is essential for eukaryotic DNA replication. (ox.ac.uk)

1997). "HsMCM6: a new member of the human MCM/P1 family encodes a protein homologous to fission yeast Mis5. (bionity.com)
BCL2 associated x protein, highly homologous Bcl-2 family members lacking the BH4 domain. (nih.gov)

As major regulators of DNA replication in eukaryotes, minichromosome maintenance (MCM) proteins play an important role in the initiation and extension of DNA replication. (nih.gov)
These proteins also appear to be simpler versions of those found in eukaryotes. (york.ac.uk)
Moreover, the flagellum is also a widely conserved organelle in eukaryotes and a defining feature of the last eukaryotic common ancestor 9 , but not yet analysed by genome-wide protein localization mapping using microscopy. (nature.com)

The Human Protein Atlas database was used to analyze the protein expression of MCMs in normal and tumor tissues. (nih.gov)

Background: Minichromosome maintenance protein 7 (MCM7) is a downstream of human epidermal growth receptor (HER1) signaling. (unicatt.it)
E.coli-derived human MCM7 recombinant protein (Position: D526-V719). (thermofisher.com)

Here we characterize C17orf53/MCM8IP, an OB-fold containing protein that binds ssDNA, as a DNA repair factor involved in HR. MCM8IP-deficient cells exhibit HR defects, especially in long-tract gene conversion, occurring downstream of RAD51 loading, consistent with a role for MCM8IP in HR-dependent DNA synthesis. (nih.gov)

Tat-interacting protein 30 (TIP30), a transcriptional repressor, possesses antitumor effect in different cancer cells. (cancerindex.org)
Nucleolin (NCL), involved in ribosome assembly, pre-mRNA metabolism, cytoplasmic RNA stability, and nucleo-cytoplasmic transport as well as ORF57, an mRNA transcript accumulation (MTA) viral early protein involved in posttranscriptional regulation, splicing of viral RNA transcripts, RNA stability, and transcriptional activation, bound specifically to ORF59. (unr.edu)

Eukaryotic LYR proteins interact with mitochondrial protein complexes. (nih.gov)
These complexes function in DNA repair pathways, primarily DNA MISMATCH REPAIR , where MutL/MLH1 and the MUTS DNA MISMATCH-BINDING PROTEIN are targeted to damaged DNA . (nih.gov)

Minichromosomes have also been successfully inserted into yeast and animal cells. (wikipedia.org)
A novel human Mcm protein: homology to the yeast replication protein Mis5 and chromosomal location. (bionity.com)

Description: A sandwich ELISA kit for detection of Internexin Neuronal Intermediate Filament Protein Alpha from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (jemsec.com)
Description: A sandwich ELISA kit for detection of Hydrogen Voltage Gated Channel Protein 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (lipidx.org)

Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. (thermofisher.com)
Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. (utsouthwestern.edu)

DNA repair proteins that include the bacterial MutL protein and its eukaryotic homologs. (nih.gov)

TP53-induced gene 3 protein. (nih.gov)
What does this gene/protein do? (cancerindex.org)
Description of the protein which includes the UniProt Function and the NCBI Gene Summary. (nih.gov)

also called Cdc2) cyclin-dependent protein kinase. (nih.gov)
CDK - a family of cyclin-dependent protein kinases. (nih.gov)
also called CCNB1) cyclin B, a cell cycle inducible protein, prominent in mitosis, which activates cdks. (nih.gov)
also called CCNA1) cyclin A, a cell cycle inducible protein, prominent in S-phase, which activates cdks. (nih.gov)

Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture. (umassmed.edu)

Familia de proteínas que en un principio fueron identificadas en el SACCHAROMYCES CEREVISIAE como elementos esenciales para el mantenimiento de la estructura de microcromosomas00. (bvsalud.org)
A family of proteins that were originally identified in SACCHAROMYCES CEREVISIAE as being essential for maintaining the structure of minichromosomes00. (bvsalud.org)

1. Cell cycle and apoptosis regulatory proteins, proliferative markers, cell signaling molecules, CD209, and decorin immunoreactivity in low-grade myxofibrosarcoma and myxoma. (nih.gov)
Overexpression of Tat-interacting protein 30 inhibits the proliferation, migration, invasion and promotes apoptosis in bladder cancer cells. (cancerindex.org)
The expression of TIP30, cell cycle proteins, migration-related proteins, and cell apoptosis-related proteins was assessed by Western blotting. (cancerindex.org)

By comparing LCM cell type preparations, 31 proteins were exclusively found in early stage cervical cancer ( n = 11) when compared with healthy epithelium and stroma, based on criteria that address specificity in a restrictive "all-or-nothing" way. (oncotarget.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Internexin Neuronal Intermediate Filament Protein Alpha (INa) in Tissue homogenates, cell lysates and other biological fluids. (jemsec.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Internexin Neuronal Intermediate Filament Protein Alpha (INa) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (jemsec.com)
Here, using fluorescence microscopy and cell lines expressing endogenously tagged proteins, we mapped the subcellular localization of 89% of the T. brucei proteome, a resource we call TrypTag. (nature.com)
Protein localization offers insights into organelle subdomains/dynamics and cell-cycle-dependent localization changes. (nature.com)

Part of the minichromosome maintenance protein (MCM) family, MCM8/9 is directly linked to ovarian insufficiency, infertility and cancers including ovarian, testicular and colon cancers, yet little is known about how this enzyme works and its connection to disease. (ecancer.org)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hydrogen Voltage Gated Channel Protein 1 (HVCN1) in Tissue homogenates and other biological fluids. (lipidx.org)

In 2007, scientists reported success in assembling minichromosomes in vitro using the de novo method. (wikipedia.org)

a protein kinase that inhibits Cdk1. (nih.gov)
ataxia telangiectasia mutated) a protein kinase that signals the presence of certain types of DNA damage. (nih.gov)

The structure of the MEF2S domain is entirely different from that of the equivalent SAM domain in SRF and MCM1, accounting for the absence of cross-reactivity with other proteins that interact with these transcription factors. (nih.gov)

The protein comprises two domains: a MADS-box (residues 1-58) and a MEF2S domain (residues 59-73). (nih.gov)

a tumor suppressor protein often mutated in human cancers. (nih.gov)
retinoblastoma protein, a tumor suppressor that binds to the E2F family of transcription activators. (nih.gov)

Scope includes mutations and abnormal protein expression. (cancerindex.org)
yet, we don't know why we get certain diseases when there are mutations in this protein," Trakselis said. (ecancer.org)

By Bonferroni correction for multiple testing, 30 proteins were significantly up-regulated between early stage cervical cancer and healthy control, including six members of the MCM protein family. (oncotarget.com)
After a less stringent Benjamini-Hochberg correction for multiple testing, we found that the abundances of 319 proteins were significantly different between early stage cervical cancer and healthy controls. (oncotarget.com)

This review also discusses alternative proposals, including cellular processes during which alternative forks may be utilized, and new biochemical studies with purified proteins that are aimed at reconstituting leading and lagging strand DNA synthesis separately and as an integrated replication fork. (nih.gov)

In this work it was demonstrated how temperature dependent SPR and FCS measurements can be performed and evaluated to determine thermodynamic data of DNA-protein interactions. (uni-bayreuth.de)

Minichromosomes may be created by natural processes as chromosomal aberrations or by genetic engineering. (wikipedia.org)
Producing minichromosomes by genetic engineering techniques involves two primary methods, the de novo (bottom-up) and the top-down approach. (wikipedia.org)
This approach has been attempted for the introduction of minichromosomes into maize for the possibility of genetic engineering, but success has been limited and questionable. (wikipedia.org)
Minichromosomes: The second generation genetic engineering tool" (PDF). (wikipedia.org)

Anatomy5

Organisms4

Chemicals and Drugs27

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes12

Disciplines and Occupations1

Information Science2

Peptides1

Chromosome11

MCM44

MCM27

Chromosomal Proteins2

Histone3

Hexameric3

Melanocortin 2 receptor accessory protein1

MCM64

Complex14

Mcm101

Homologous2

Eukaryotes3

MCMs1

MCM72

Deficient1

Transcriptional2

Complexes2

Yeast2

ELISA Kit2

Binding2

Eukaryotic1

Gene3

Cyclin4

Chromatin1

Cerevisiae2

Apoptosis3

Cell5

Enzyme2

Vitro1

Kinase2

Interact1

Domains1

Tumor2

Mutations2

Cervical cancer2

Cellular1

Data1

Genetic4