- The minichromosome maintenance proteins were named after a yeast genetics screen for mutants defective in the regulation of DNA replication initiation. (wikipedia.org)
- The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. (nih.gov)
- This table lists all participants of the complex (proteins, small molecules, nucleic acids, etc.) and their respective stoichiometry. (yeastgenome.org)
- In particular, ProEx C is an immunohistochemical cocktail containing antibodies direct against topoisomerase IIα (TOP2A) and minichromosome maintenance 2 (MCM2) proteins. (medsci.org)
- A hexameric protein complex of minichromosome maintenance proteins. (bvsalud.org)
- Chromosome area maintenance 1 proteins (CRM1 or known as XPO1) can be a member of the importin superfamily of nuclear move receptors (karyopherins). (cancerhugs.com)
- Chromosome association of minichromosome maintenance proteins in Drosophila mitotic cycles. (colorado.edu)
- The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer. (yeastgenome.org)
- Using cryo-electron microscopy and biochemical analysis we discovered that an interaction between the HBRCT domain of Dbf4 with Mcm2 serves as an anchoring point, which supports binding of DDK across the MCM2-7 double-hexamer interface and phosphorylation of Mcm4 on the opposite hexamer. (imperial.ac.uk)
- The Mcm ring is remodeled in CMG relative to the inactive Mcm2-7 double hexamer. (rcsb.org)
- Soluble Mcm2-7 hexamer forms a flexible left-handed open-ringed structure stabilised by Cdt1 prior to its loading onto chromatin, one at a time. (wikipedia.org)
- It has been unclear at the molecular level how Cdc6 activates ORC, converting it to an active recruiter of the Mcm2-7 hexamer, the core of the replicative helicase. (imperial.ac.uk)
- Eukaryotic MCM consists of six gene products, Mcm2-7, which form a heterohexamer. (wikipedia.org)
- Later on, others identified Mcm4, Mcm6 and Mcm7 in yeasts and other eukaryotes based on homology to Mcm2p, Mcm3p and Mcm5p expanding the MCM family to six, subsequently known as the Mcm2-7 family. (wikipedia.org)
- Mcm1 and Mcm10 are also involved in DNA replication, directly or indirectly, but have no sequence homology to the Mcm2-7 family. (wikipedia.org)
- During G1 phase, the two head-to-head Mcm2-7 rings serve as the scaffold for the assembly of the bidirectional replication initiation complexes at the replication origin. (wikipedia.org)
- During S phase, the Mcm2-7 complex forms the catalytic core of the Cdc45-MCM-GINS helicase - the DNA unwinding engine of the replisome. (wikipedia.org)
- During the G1 phase of the cell cycle, Cdc6 is recruited by ORC to form a launching pad for the loading of two head-to-head Mcm2-7 hexamers, also known as the pre-replication complex (pre-RC). (wikipedia.org)
- The structure of the ORC-Cdc6-Cdt1-MCM (OCCM) intermediate formed after the loading of the first Cdt1-Mcm2-7 heptamer indicates that the winged helix domain at the C-terminal extensions (CTE) of the Mcm2-7 complex firmly anchor onto the surfaces created by the ORC-Cdc6 ring structure around origin DNA. (wikipedia.org)
- The fusion of the two head-to-head Mcm2-7 hexamers is believed to be facilitated by the removal of Cdt1, leaving the NTDs of the two MCM hexamers flexible for inter-ring interactions. (wikipedia.org)
- The loading of MCM2-7 onto DNA is an active process that requires ATP hydrolysis by both Orc1-6 and Cdc6. (wikipedia.org)
- Once pre-RC formation is complete, Orc1-6 and Cdc6 are no longer required for MCM2-7 retention at the origin, and they are dispensable for subsequent DNA replication. (wikipedia.org)
- Upon entry into S phase, the activity of the CDKs and the Dbf4-dependent kinase (DDK) Cdc7 promotes the assembly of replication forks, likely in part by activating MCM2-7 to unwind DNA. (wikipedia.org)
- When the replication fork encounters lesions in the DNA, the S-phase checkpoint response slows or stops fork progression and stabilizes the association of MCM2-7 with the replication fork during DNA repair. (wikipedia.org)
- Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. (nih.gov)
- The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. (nih.gov)
- Global gene expression profiling of mouse medulloblastomas and bioinformatics analyses of microRNA targets suggest that minichromosome maintenance complex component 2 (MCM2) is a likely target gene of miR-31 in suppressing cell growth. (oncotarget.com)
- MCM2-7 complexes unwind the double stranded DNA at the origins, recruit DNA polymerases and initiate DNA synthesis. (yeastgenome.org)
- The N-terminus of Spt16 anchors FACT to MCM2-7 for parental histone recycling. (yeastgenome.org)
- DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7. (yeastgenome.org)
- The Dbf4-dependent Cdc7 kinase (DDK) initiates replisome assembly by phosphorylating the MCM2-7 replicative helicase at the N-terminal tails of Mcm2, Mcm4 and Mcm6. (imperial.ac.uk)
- In summary, our work provides fundamental insights into DDK structure, control and selective activation of the MCM2-7 helicase during DNA replication. (imperial.ac.uk)
- Cdc6 binding rearranges a short α-helix in the Orc1 AAA+ domain and the Orc2 WHD, leading to the activation of the Cdc6 ATPase and the formation of the three sites for the recruitment of Mcm2-7, none of which are present in ORC alone. (imperial.ac.uk)
- Mutational analyses show that the MCM2 HBD is required for MCM2-7 histone-chaperone function and normal cell proliferation. (cornell.edu)
- The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)
- Figure 2: Western blot analysis using MCM2 mouse mAb against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), Hela (8) and MCF-7 (9) cell lysate. (promab.com)
- The CMG helicase is composed of Cdc45, Mcm2-7 and GINS. (rcsb.org)
- The N-terminal regions of Mcm2-7, braced by Cdc45-GINS, form a rigid platform upon which the AAA+ C domains make longitudinal motions, nodding up and down like an oil-rig pumpjack attached to a stable platform. (rcsb.org)
- Bell SD and Botchan MR (2013) The minichromosome maintenance replicative helicase. (yeastgenome.org)
- Site selection for replication origins is carried out by the Origin Recognition Complex (ORC), a six subunit complex (Orc1-6). (wikipedia.org)
- Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-O. (yeastrc.org)
- One of the components of the network is HMMR, encoding a centrosome subunit, for which we demonstrate previously unknown functional associations with the breast cancer-associated gene BRCA1. (gsea-msigdb.org)
- Essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells, associates with the origins of DNA replication to form part of the pre-replicative complex. (yeastgenome.org)
- Activation of the MCM complex at origins by cyclin-dependent kinases and the CDC7 protein kinase (P06243) leads to initiation of DNA synthesis. (yeastgenome.org)
- The pre-replication complex (pre-RC) assembly or the DNA replication licensing is the first step in DNA replication initiation, characterized by the sequential recruitment of ORCs, Cdc6, Cdt1 and MCMs to the DNA replication origins to form the pre-RC at the end of mitosis ( Bell and Dutta 2002 ). (intechopen.com)
- The minichromosome maintenance protein complex (MCM) is a DNA helicase essential for genomic DNA replication. (wikipedia.org)
- The activation of replication checkpoint may slow down DNA replication and improve DNA replication fidelity, which increases the maintenance of genomic stability and counteracts carcinogenesis. (nih.gov)
- The group of benign tumours included 164 pleomorphic adenomas, 51 Warthin's tumours, 7 basal cell adenomas, 2 lipomas as well as 5 other different tumours. (researchgate.net)
- They form into a protein complex that has helicase activity and is involved in a variety of DNA-related functions including replication elongation, RNA transcription, chromatin remodeling, and genome stability. (bvsalud.org)
- This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. (promab.com)
- The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. (nih.gov)
- Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc. (yeastrc.org)
- Although immune checkpoint inhibitors (ICIs), in particular blockade of PD-1/PD-L1, show promising therapy response in some cancers, they are less effective in PCa which may be poor infiltration of cytotoxic T-cells [ 7 - 9 ]. (thno.org)
- The functional regulation of cells is a complex and dynamic process. (nature.com)
- This diagram displays Gene Ontology terms (green) and subunits (blue) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). (yeastgenome.org)
- Cdt1p, through its interaction with Mcm6p, is required for the formation, nuclear accumulation and chromatin loading of the MCM complex. (yeastgenome.org)
- Syroegin, E. A. , Aleksandrova, E. V. , and Polikanov, Y. S. (2022) Insights into the ribosome function from the structures of non-arrested ribosome-nascent chain complexes . (cornell.edu)
- Gene Ontology (GO) terms that describe the function of a complex, the biological process in which it participates, or its cellular location. (yeastgenome.org)
- This network shows higher connectivity than expected by chance, suggesting that its components function in biologically related pathways. (gsea-msigdb.org)
- Mechanistically, the complex consisting of YAP and transcription factors promotes the expression of genes involved in DNA replication, cell cycle regulation, chromosomal segregation, but also in the control of cellular stemness. (biomedcentral.com)
- the process that governs the early embryonic patterning of the Drosophila body plan is now the best understood example of a complex cascade of transcriptional regulation during development [ 14 , 15 ]. (biomedcentral.com)
- Here we explore combinations of different classes of therapeutic anti-HIV-1 RNAs expressed from within the context of an intronic MCM7 (minichromosome maintenance complex component-7) platform that naturally harbors 3 microRNAs (miRNAs). (ca.gov)
- Better understanding is also required of how mutations in cancer genes affect their products in the context of complex cellular networks. (gsea-msigdb.org)
- The accurate diagnosis is crucial for the appropriate management and routinary controls for UC are necessary once the diagnosis is made [ 4 - 7 ]. (medsci.org)
- Functional control of Eco1 through the MCM complex in sister chromatid cohesion. (yeastgenome.org)
- Macromolecular complex annotations are imported from the Complex Portal . (yeastgenome.org)
- Note: No diagram is shown ("No shared annotations") if there are less than 2 shared annotations (either GO terms or subunits of other complexes) between this complex and any other complexes. (yeastgenome.org)
- protein_coding" "Cz03g18100.t1","No alias","Chromochloris zofingiensis","Spindle assembly checkpoint component Mad1 [Interproscan]. (ntu.edu.sg)
- Background Chromosome Area Maintenance 1 (CRM1) is a nuclear exporter and its inhibitor has anti-tumor activity in different cancers. (cancerhugs.com)
- Simplicity of potato growing and ease of industrial production of pure potato starch makes it a necessary component and starting compound in food and chemical industries and determines valuability of an exhaustive study of all aspects of manufacturing and commercial application of potato starch is necessary. (peerj.com)
- Two classes of mcm mutants were identified: Those that affected the stability of all minichromosomes and others that affected the stability of only a subset of the minichromosomes. (wikipedia.org)
- Data from ITS rDNA and the genes coding for translation elongation factor 1-α and DNA replication licensing factor mini-chromosome maintenance complex 7 support the distinctness of Mycoblastus s. str. (cambridge.org)