• hydroxylase activity
  • At a substrate concentration of 20 μM, propofol hydroxylase activity was significantly lower ( p = .032) in greyhound microsomes (1.7 ± 0.4 nmol/mg/min) compared with beagle microsomes (5.1 ± 1.3 nmol/mg/min) but was not statistically different ( p = .42) compared with mixed-breed microsomes (3.1 ± 1.2 nmol/mg/min). (aspetjournals.org)
  • 3) Kinetic analysis of tirilazad hydroxylase activity in three human livers resulted in an apparent Km of 2.12, 1.68 and 1.66 microM, and Vmax = 0.85, 0.44 and 3.45 (nmol/mg protein/min) for HL14, HL17 and HL21, respectively. (aspetjournals.org)
  • assays
  • Using isotope incorporation assays, we measured the transformation of the precursors, [3 alpha- 3H]lathosterol and [1,2-3H]7-dehydrocholesterol into cholesterol by liver microsomes from seven controls and four Smith-Lemli-Opitz homozygous subjects. (jci.org)
  • potent
  • Meperidine is an opioid analgesic metabolized in the liver by N -demethylation to normeperidine, a potent stimulant of the central nervous system. (aspetjournals.org)
  • protein
  • The introduction of the double bond in lathosterol at C-5 to form 7-dehydrocholesterol that is catalyzed by lathosterol-5-dehydrogenase was equally rapid in controls and homozygotes liver microsomes (120 +/- 8 vs 100 +/- 7 pmol/mg protein per min, P = NS). (jci.org)
  • The concentration of 5'nucleotidase protein in the blood is often used as a liver function test in individuals that show signs of liver problems. (wikipedia.org)
  • In other words, the test is used to determine if elevated protein levels are due to skeletal damage or liver damage. (wikipedia.org)
  • kidney
  • Use by those with significant kidney problems, liver problems, or who are pregnant is not recommended. (wikipedia.org)