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  • Second
  • The efficiencies of two-photon-excited fluorescence (TPEF) and second harmonic generation (SHG) were studied for various pulse durations, measured at the sample, ranging from approximately 400 fs to sub-20 fs. (ubc.ca)
  • 1995
  • The first experimental measurement of TPCD was performed in 1995 using a fluorescence based technique (FD-TPCD), but it was not until the introduction of the double L-scan technique in 2008 by Hernández and co-workers, that a more reliable and versatile technique to perform TPCD measurements became available. (wikipedia.org)
  • detection
  • The featured discussion is intended to aid in understanding the basics of light detection and to provide a guide for selecting a suitable detector for specific applications in fluorescence microscopy. (fsu.edu)
  • J. Enderlein, T. Ruckstuhl, S. Seeger: Highly Efficient Optical Detection of Surface-Generated Fluorescence. (wikipedia.org)
  • However, if the excitation pulse or detection response is wide, the measured fluorescence, d(t), will not be purely exponential. (wikipedia.org)
  • Although the use of coefficients can significantly improve the reliability of colocalization detection, it depends on the number of factors, including the conditions of how samples with fluorescence were prepared and how images with colocalization were acquired and processed. (wikipedia.org)
  • 1996
  • Although this near-wtGFP was fluorescent, it had several drawbacks, including dual peaked excitation spectra, pH sensitivity, chloride sensitivity, poor fluorescence quantum yield, poor photostability and poor folding at 37 °C. The first reported crystal structure of a GFP was that of the S65T mutant by the Remington group in Science in 1996. (wikipedia.org)
  • tomography
  • Starting in 1994, LSFM was developed as orthogonal plane fluorescence optical sectioning microscopy or tomography (OPFOS) mainly for large samples and later as the selective/single plane illumination microscopy (SPIM) also with sub-cellular resolution. (wikipedia.org)
  • Optics
  • Using an aspheric lens for excitation of a sample with laser light, fluorescence emitted by the specimen is collected above the critical angle of total internal reflection selectively and directed by a parabolic optics onto a detector. (wikipedia.org)
  • illumination
  • Read more about photomicrography under fluorescence illumination conditions can present a unique set of circumstances that may pose special problems for the microscopist. (fsu.edu)
  • Fluorescence microscopy can be combined with contrast enhancing techniques such as DIC illumination to minimize the effects of photobleaching by locating a specific area of interest in a specimen using DIC . (fsu.edu)
  • To minimize photobleaching, fluorescence microscopy can be combined with phase contrast illumination. (fsu.edu)
  • This introduced an illumination scheme into fluorescence microscopy, which has already been used successfully for dark field microscopy under the name ultramicroscopy. (wikipedia.org)
  • In this type of microscopy, the illumination is done perpendicularly to the direction of observation (see schematic image at the top of the article). (wikipedia.org)
  • light
  • Epi-fluorescence, or incident light fluorescence, has now become the method of choice in many applications and comprises a large part of this tutorial. (fsu.edu)
  • Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user. (waterstones.com)
  • Light sheet fluorescence microscopy (LSFM) is a fluorescence microscopy technique with an intermediate-to-high optical resolution, but good optical sectioning capabilities and high speed. (wikipedia.org)
  • Because LSFM scans samples by using a plane of light instead of a point (as in confocal microscopy), it can acquire images at speeds 100 to 1000 times faster than those offered by point-scanning methods. (wikipedia.org)
  • This way a thin sheet of light or lightsheet is created in the focal region that can be used to excite fluorescence only in a thin slice (usually a few micrometers thin) of the sample. (wikipedia.org)
  • The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. (wikipedia.org)
  • These characteristics drive other properties, including the photobleaching or photoresistance (loss of fluorescence upon continuous light excitation). (wikipedia.org)
  • He also did pioneering work on plasmodesmata, stomatal function, and in the development of techniques useful for answering questions using light and electron microscopy. (wikipedia.org)
  • Therefore, for fluorescence microscopy DAPI is excited with ultraviolet light and is detected through a blue/cyan filter. (wikipedia.org)
  • Outside of analytical fluorescence light microscopy DAPI is also popular for labeling of cell cultures to detect the DNA of contaminating mycoplasma or virus. (wikipedia.org)
  • sensitivity
  • Lumenera's INFINITY Fluorescence Series cameras have a new sleek black enclosure, and feature Sony CCD sensors to offer high dynamic range, high sensitivity, and optional thermoelectric cooling, resulting in excellent quality images and fast preview speeds. (prweb.com)
  • Recent improvements in methods of single-particle fluorescence tracking have permitted detailed studies of molecular motion on the nanometer scale due to its high selectivity, sensitivity, simplicity, and fastness [ 8 ]. (hindawi.com)
  • The high microscopy sensitivity resolves the signal of a single fluorescence-labeled bimolecular within a living cell. (hindawi.com)
  • techniques
  • The unit on main campus specializes in both single cell and population statistics as well as imaging of a wide variety of sample types using fluorescence based techniques. (sun.ac.za)
  • In addition to the element to which fluorescence probes are attached, there are two general classes of immunofluorescence techniques: primary and secondary. (wikipedia.org)
  • secondary
  • The chromosomes were treated with primary antibodies to CDC6, a nuclear protein, and immunolabeled with secondary antibody fragments conjugated to Alexa Fluor 586 (red fluorescence). (fsu.edu)
  • images
  • In fluorescence images of mitosis, the two poles of the spindle and the spindle fibers are clearly visible, as are the asters , which radially migrate away from the metaphase plate. (fsu.edu)
  • In addition, due to the tendency of fluorescence images to contain a certain amount of out-of-focus signal, and poisson shot and other noise, they usually require pre-processing prior to quantification. (wikipedia.org)