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  • Moreover
  • Moreover, restoration of 4Pi confocal images of the same object with alternate phases is introduced as a powerful test for (nonlinear) image restoration. (osapublishing.org)
  • specimen
  • Fluorescence emission gathered from the specimen is filtered through a confocal pinhole aperture to reject light that originates from regions removed from the focal plane. (zeiss.com)
  • images
  • Linear lobe deconvolution as well as nonlinear restoration of 4Pi confocal raw data with their point-spread functions (PSF's) leads to almost-identical images, irrespective of whether the 4Pi confocal PSF relies on constructive or destructive interference. (osapublishing.org)
  • Fluorescence microscopy is unmatched in its ability to provide images with chemical specificity. (photonics.com)
  • Good optical sectioning, often referred to as good depth or z resolution, is popular in modern microscopy as it allows the three-dimensional reconstruction of a sample from images captured at different focal planes. (wikipedia.org)
  • The purpose of using coefficients is to characterize the degree of overlap between images, usually two channels in a multidimensional microscopy image recorded at different emission wavelengths. (wikipedia.org)
  • researchers
  • Today, more and more researchers are interested in linking macroscopic properties and behavior - such as memory learning - with microscopy at the single cell and subcellular level, as well as studying molecular events such as gene control and neuronal activity like excitatory and inhibitory signaling. (photonics.com)
  • high-resolution
  • It allows an endoscope to perform in situ, high-resolution fluorescence microscopy imaging in real time, at the cellular and subcellular levels, via an instrument small and flexible enough to reach all the way into the tiny alveoli in the lungs. (photonics.com)
  • emission
  • In fluorescence microscopy, colocalization refers to observation of the spatial overlap between two (or more) different fluorescent labels, each having a separate emission wavelength, to see if the different "targets" are located in the same area of the cell or very near to one another. (wikipedia.org)
  • Methods
  • It often involves a biopsy for subsequent laboratory analysis with various methods that include visual microscopy . (photonics.com)
  • Up to now, most frequently used methods to quantify colocalization calculate the statistical correlation of pixel intensities in two distinct microscopy channels. (wikipedia.org)
  • development
  • Fortunately, a recent development is set to change the cost-performance balance in this space as manufacturers develop fast confocal instruments for rapid fixed and live-cell imaging. (photonics.com)
  • Electron Microscopy
  • This page of the WWW-Virtual Library covers all aspects of light microscopy, electron microscopy and other forms of microscopy. (ou.edu)
  • As an innovator, his research includes development state-of-the art of instrumentation and techniques for atomic resolution x-ray & electron spectroscopy, and electron microscopy. (wikipedia.org)
  • Zaluzec has made wide-ranging contributions to the field of electron microscopy and microanalysis beginning with his seminal work on quantitative x-ray and electron spectroscopy, which has been disseminated throughout the scientific and academic communities through hundreds of lectures, short courses and/or seminars at scientific conferences and meetings around the globe. (wikipedia.org)
  • He is the recipient of numerous awards from professional societies such as the August Köhler Award from the State Microscopical Society of Illinois, Distinguished Service Awards from the MSA and the Australian Microscopy Society, Member of the Year Award from the Midwest Society of Electron Microscopists, Presidential Citation from the Australian Society for Electron Microscopy, the Science Digest "One of America's 100 Brightest Scientists" Award and two R&D 100 Awards. (wikipedia.org)
  • decades
  • Over the next few decades the microscopy division progressed considerably with the introduction of groundbreaking polarising and stereomicroscopes in addition to metrology products for measuring and inspection. (wikipedia.org)
  • resolution
  • He developed Lorentz STEM imaging, High Angular Resolution Electron Channeling X-ray Spectroscopy (HARECXS), High Angular Resolution Electron Channeling Electron Spectroscopy (HARECES), Position Resolved Diffraction, as well as his invention of the scanning confocal electron microscope and the π steradian Transmission X-ray Detector, for which he was given the R&D 100 Awards in 2003 and 2010 respectively. (wikipedia.org)
  • r = 0.4 λ N A {\displaystyle r={\frac {0.4\lambda }{\mathrm {NA} }}} Also common in the microscopy literature is a formula for resolution that treats the above-mentioned concerns about contrast differently. (wikipedia.org)
  • Nikon's new N-SIM microscopy system can produce two times conventional resolution by combining Structured Illumination Microscopy technology licensed from UCSF based on Nikon's Eclipse Ti research inverted microscope. (wikipedia.org)
  • Science
  • He was one of the earliest to realize the potential impact of the Internet on science and established the TelePresence Microscopy Collaboratory, which has served as a model for outreach to both the scientific and education communities providing unencumbered access to scientific resources. (wikipedia.org)