gravity-assisted microdissection
- Gravity-assisted microdissection(GAM) is one of the laser microdissection methods. (wikipedia.org)
- ION LMD system is one of the laser microdissection systems and a name of device that follows Gravity-Assisted Microdissection method, also known as GAM method. (wikipedia.org)
chromosome
- Three region-specific libraries for the entire human chromosome 18 were constructed using microdissection and MboI linker-adaptor microcloning techniques. (springer.com)
- Different kinds of techniques involve microdissection: Chromosome microdissection - use of fine glass needle under a microscope to remove a portion from a complete chromosome. (wikipedia.org)
- Chromosome microdissection is a technique that physically removes a large section of DNA from a complete chromosome. (wikipedia.org)
- Chromosome microdissection is a specialized way of isolating these regions by removing the DNA from the band and making that DNA available for further study. (wikipedia.org)
- To prepare cells for chromosome microdissection, a scientist first treats them with a chemical that forces them into metaphase: a phase of the cell's life-cycle where the chromosomes are tightly coiled and highly visible. (wikipedia.org)
- Guan XY, Meltzer PS, Trent JM (1994) Rapid generation of whole chromosome painting probes (WCPs) by chromosome microdissection. (springer.com)
- 2002) The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals. (springer.com)
- 1992) Rapid generation of region specific probes by chromosome microdissection and their application. (springer.com)
- 1999) Laser microdissection and laser pressure catapulting as an approach for the generation of chromosome specific paint probes. (springer.com)
isolation of specific cells
- Laser capture microdissection (LCM) is an automated sample preparation technique that enables isolation of specific cells from a mixed population under microscopic visualization. (thermofisher.com)
- Laser capture microdissection enables gentle, selective isolation of specific cells from heterogeneous tissue, protecting biomolecular integrity and preserving profiles of complex samples for more accurate downstream results. (thermofisher.com)
- Laser capture microdissection allows for rapid isolation of specific cells in four simple steps. (thermofisher.com)
enables
- Laser capture microdissection is a powerful technique that enables oncology researchers to compare molecular profiles of tumor tissue to surrounding nontumor cells. (thermofisher.com)
- Laser capture microdissection (LCM) is a technology that enables scientists to examine the processes of individual cells. (springer.com)
- also known as Laser Capture Microdissection or LCM) enables users to isolate specific single cells or entire areas of tissue. (leica-microsystems.com)
- Laser microdissection (LMD) technology enables highly specific gene expression analyses of biologically relevant questions at cell- or tissue-specific resolution. (wur.nl)
Methods
- Murray, G. I. and Curran, S. Laser Capture Microdissection: Methods and Protocols . (springer.com)
- Noncontact laser catapulting: a basic procedure for functional genomics and proteomics, in Laser Capture Microscopy and Microdissection , Methods in Enzymology Vol. 365, Conn, P. M. (ed. (springer.com)
- Wittliff, J. L. and Erlander, M. G. Laser capture microdissection and its applications in geonomics and proteomics, in Laser Capture Microscopy and Microdissection , Methods in Enzymology Vol. 365, Conn, P. M. (ed. (springer.com)
- Craven, R. A. and Banks, R. E. Use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis, in Laser Capture Microscopy and Microdissection , Methods in Enzymology Vol. 365, Conn, P. M., ed. (springer.com)
- These methods include: Micropipetting Cytoplasmic aspiration Laser capture microdissection. (wikipedia.org)
morphology
- The technique causes no apparent alteration to the morphology of the chosen cells, which can be directly visualized after microdissection. (thermofisher.com)
populations
- The precise and contact-free laser microdissection system isolates single cell populations from heterogenous samples. (zeiss.com)
ultraviolet
- The ArcturusXT™ microdissection system offers a unique combination of infrared (IR) laser-enabled laser capture microdissection (LCM) and ultraviolet (UV) laser cutting in one platform. (thermofisher.com)
gentle
- IR laser provides a more gentle approach to microdissection. (wikipedia.org)
Cells
- Laser Microdissection is the contamination-free method for separating and collecting cells of interest out of tissue samples or cell cultures. (zeiss.com)
- Laser capture microdissection (LCM) is a powerful technique that has become instrumental to researchers interested in comparing the molecular profiles of tumor tissue to surrounding non-tumor cells, thereby unlocking hidden molecular signatures in individual patient tumor samples and leading the way for tailored treatment and personalized medicine. (thermofisher.com)
- Laser microdissection - use of a laser through a microscope to dissect selected cells. (wikipedia.org)
- The Veritas Microdissection fluorescence package includes a high-sensitivity, variable integration time, color CCD video camera and red, blue and green filter cubes for the easy identification and precise dissection of fluorescently stained cells. (bio-medicine.org)
- Laser capture microdissection (LCM), also called microdissection, laser microdissection (LMD), or laser-assisted microdissection (LMD or LAM), is a method for isolating specific cells of interest from microscopic regions of tissue/cells/organisms (dissection on a microscopic scale with the help of a laser). (wikipedia.org)
- Laser-capture microdissection (LCM) is a method to procure subpopulations of tissue cells under direct microscopic visualization. (wikipedia.org)
molecular
- Laser capture microdissection: molecular analysis of tissue. (springer.com)
Utilize
- More recent technologies utilize non-contact microdissection. (wikipedia.org)
characterization
- Müller-Navia J, Nebel A, Schleiermacher E. (1995) Complete and precise characterization of marker chromosomes by application of microdissection in prenatal diagnosis. (springer.com)
needle
- Commercially, many microdissection needle systems are available, such as Stryker Colorado® microdissection needle (CMN) (Stryker-Leibinger, Freiburg, Germany) and optimicro™ microdissection needles. (bioportfolio.com)
- Colorado® microdissection needle (CMN) combine the advantages of scalpel and electrosurgery. (bioportfolio.com)
- The primary feature of the Colorado® microdissection needle is the ultra-sharp tungsten tip that delivers the wave-form from the electrosurgery generator to a very small spot. (bioportfolio.com)
microscope
- Microdissection refers to a variety of techniques where a microscope is used to assist in dissection. (wikipedia.org)
Techniques
- Brain Microdissection Techniques (A. C. Cuello, ed. (springer.com)
- Veritas Microdissection offers the flexibility to use the slide and tissue preparation techniques best for your applications. (bio-medicine.org)
biopsy
- Laser capture microdissection (LCM) has been applied on endomyocardial biopsy samples from patients with IC to assess the presence of EBV genome in separately dissected lymphocytes and myocytes. (ahajournals.org)
Applications
- The petri dish stage insert has opened the door for live-cell microdissection applications such as stem cell research and other rare-cell isolations. (thermofisher.com)
- Such applications have been demonstrated in a series of similarly constructed microdissection libraries from other regions of the human genome. (springer.com)
- to avoid any image alterations from your traditional illumination technique, even if the system is used for laser microdissection independent applications. (leica-microsystems.com)
researchers
- Laser Capture Microdissection (LCM) is the method of choice for thousands of researchers worldwide. (bio-medicine.org)
instrument
- Dedicated for contact-free micromanipulation this modular instrument combines the benefits of laser microdissection with optical tweezers. (zeiss.com)
- Veritas Microdissection is the only instrument to offer both LCM and Laser Cutting, providing unmatched flexibility. (bio-medicine.org)
Analysis
- Here, we demonstrate an approach to conduct laser microdissection of the lung alveolar duct for single-cell PCR analysis. (frontiersin.org)
- This non-contact laser microdissection system makes cell isolation for further genetic analysis possible. (wikipedia.org)
specific
- Scalenghe F, Turco E, Edstrom JE, Pirrotta V, Melli M.: Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. (wikipedia.org)
rapid
- First, computer-controlled laser microdissection of tissue has facilitated the rapid isolation of small anatomic units within complex tissues. (frontiersin.org)
system offers
- The Veritas Microdissection system offers unlimited slide processing in batch mode. (bio-medicine.org)
single cell
- Extract Unique Multi-cellular Structures Drawing tools make microdissection of unique multi-cellular structures as simple as single cell dissection. (bio-medicine.org)
samples
- Veritas Microdissection can cut and capture structures from multiple tissue sections or cytological samples with just a few mouse clicks. (bio-medicine.org)
Study
- 13 The aim of the present study was to clarify the role of EBV in patients with inflammatory cardiomyopathy by use of laser capture microdissection (LCM) to localize the viral genome in EBV-infected hearts. (ahajournals.org)
- A microdissection study. (bmj.com)
Step
- Each step of the microdissection process is documented and archivable. (bio-medicine.org)
whole
- Figure 1A shows a whole tissue sample before laser capture microdissection. (thermofisher.com)
preparation
- A layer-by-layer microdissection of the ventral part of the external and extreme capsules using Klingler's preparation also demonstrated five types of uncinate fibers that, according to their pattern, depth, and cortical terminations, were consistent with the diffusion-based UF subcomponents. (springer.com)