Flow CytometryAnalyzed microbial cellsAdhesion and viabilityConcentrations of microbialScanning electron mMicroorganismsGenomeBacterial strainsVitroMicrobiomeCellEfficiencyIntegrityStrainsBacteriaPathogensAssaysViableContaminationAntibioticCarbohydrateEvaluateGeneticAnalysesBiologicalFungalGrowthEcologyProbioticsDiversityAdditionallyInvestigateTemperatureCompositionCellsVariationSpeciesCommunitiesHigherStudyCommunityProtocolsApplicationsProvideSelectionResultsWater
Flow Cytometry4
- The first referenced work about this analytical approach was in 2003, Norwegian researchers suggest the use of Ethidium Monoazide, an azide form of Ethidium Bromide, which was used in other analytical fields as Flow Cytometry as a candidate for viability PCR. (wikipedia.org)
- Microbial cell concentrations and viability were assessed via flow cytometry and adenosine triphosphate (ATP). (stanford.edu)
- The results provide insights into how treatment train design and operation choices affect microbial water quality as well as the use of flow cytometry and ATP for online monitoring and process control. (stanford.edu)
- 1999. Approaches to the estimation of microbial viability using flow cytometry. (dbkgroup.org)
Analyzed microbial cells1
- In this study we analyzed microbial cells and biological ice nucleating particles (INPs) in smoke emitted from eight prescribed wildland fires in North Florida. (nature.com)
Adhesion and viability3
- Microbial adhesion and viability on novel CAD/CAM framework materials for implant-supported hybrid prostheses. (bvsalud.org)
- The aim of the study was to investigate the adhesion and viability of Streptococcus oralis and Candida albicans under in vitro conditions on CAD/CAM framework materials for implant-supported hybrid prostheses . (bvsalud.org)
- Saliva contains both specific and non-specific protective factors of the immune system, such as antimicrobial proteins, which can inhibit the adhesion and viability of cariogenic microorganisms. (bvsalud.org)
Concentrations of microbial2
- When compared to air sampled prior to ignition, samples of the air-smoke mixtures contained fivefold higher concentrations of microbial cells (6.7 ± 1.3 × 10 4 cells m −3 ) and biological INPs (2.4 ± 0.91 × 10 3 INPs m −3 active at temperatures ≥ −15 °C), and these data significantly positively correlated with PM 10 . (nature.com)
- The results indicate that MWF mist may contain high concentrations of microbial fragments, which are difficult to detect with traditional microbial analysis methods, such as cultivation or microscopic counting. (cdc.gov)
Scanning electron m1
- The experimental part included surface roughness (SR) and contact angle of water (CAW) measurements, followed by colony forming unit (CFU), cell viability assay and scanning electron microscopy (SEM) analyses of Strep. (bvsalud.org)
Microorganisms4
- The aim of the present study was to shed light on the real impact on biodiversity and ecosystems of Roundup(®), a major herbicide used worldwide, and the glyphosate it contains, by the study of their effects on growth and viability of microbial models, namely, on three food microorganisms (Geotrichum candidum, Lactococcus lactis subsp. (greenmedinfo.com)
- Most of the microbial research on metalworking fluid s (MWF) so far has focused on the components of microorganisms in the MWF reservoir. (cdc.gov)
- In the ScanRDI™ assay, samples are passed through a 0.45μ filter to retain microorganisms, which are then labeled with reagents to determine their viability. (europeanpharmaceuticalreview.com)
- Most of these proteins can inhibit the metabolism, adherence and cariogenic viability of microorganisms in vitro (1-3). (bvsalud.org)
Genome1
- During her summer at Scripps, Bovee worked with Luke Fisher in marine biologist Douglas Bartlett's lab, studying the effects of extreme salinity on microbial viability and genome integrity. (ucsd.edu)
Bacterial strains2
- Many microbial cultures, particularly bacterial strains, quickly succumb to genetic drift, often resulting in major phenotypic changes. (genengnews.com)
- Antibiotic treatment by humans generates strong viability selection for antibiotic-resistant bacterial strains. (bioone.org)
Vitro2
- We singled out gut transit time in an unprecedented in vitro approach by subjecting faecal microbial communities from six individuals with either short, medium or long in vivo transit times, to three different colonic transit times of 21, 32 and 63 h in the validated human gut in vitro model, SHIME. (biomedcentral.com)
- Microbial growth rates and nutrient utilisation efficiency mediate the species-specific gut microbiota response to in vitro transit time variation, which is the main driver of in vitro microbial load, metabolism and community composition. (biomedcentral.com)
Microbiome2
- and (2) that the development of host-free microbiome model systems could be useful for rapidly screening pesticides against functionally distinct microbial catalogues of interest. (researchgate.net)
- The human gut microbiome, a diverse microbial ecosystem comprising approximately 10 13 microbial cells, is widely studied with a focus on the interplay with diet and health [ 1 ]. (biomedcentral.com)
Cell15
- Cell viability assay revealed similar values for both species across materials. (bvsalud.org)
- Direct regulation of T cell function by microbial ligands through Toll-like receptors (TLR) is an emerging part of T cell biology. (mingsheng88.org)
- This study stresses the importance of a stringent purification method when investigating the part of microbial ligands in T cell function. (mingsheng88.org)
- Cell viability was also assessed by propidium iodide incorporation. (mingsheng88.org)
- No variations in cell viability were recognized after isolation with different protocols. (mingsheng88.org)
- ATP-Glo ™ Bioluminometric Cell Viability luciferase assay. (biotium.com)
- Learn more about our wide selection of assays for cell proliferation, cell viability, and apoptosis . (biotium.com)
- ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. (biotium.com)
- In this study, we investigated if de novo ATP production of cells in the VBNC state is a suitable indicator for overall cell viability that can be utilized to determine the minimum ATP inhibitory concentration (MAIC) of antibiotics and other antimicrobials. (frontiersin.org)
- This allows the instrument to preserve cell viability and outgrowth while simultaneously increasing cell deposition efficiency. (moleculardevices.com)
- Maximize efficiency, preserve cell viability, and streamline your workflow with DispenCell and CloneSelect Imager FL bundle. (moleculardevices.com)
- Transit time was identified as the single most important driver of microbial cell concentrations (52%), metabolic activity (45%) and quantitative (24%) and proportional (22%) community composition. (biomedcentral.com)
- Timely results for microbial bioburden monitoring of in-process cell culture samples are critical to the production process for recombinant proteins and other biopharmaceutical products. (europeanpharmaceuticalreview.com)
- Cell viability was assessed by CellTiter-Blue assays. (hud.ac.uk)
- Once the TA system detects the invading phage, the toxin is activated, compromising the viability of the infected bacterial cell. (lu.se)
Efficiency1
- Fibre digestion was improved by 4% ( p = .02) when the same dose of urea was infused into the abomasum compared with the rumen, while estimated microbial protein production and N efficiency were not different between a low rumen dose and high post‐ruminal dose of urea. (trouwnutrition.com)
Integrity1
- During this project period, our objectives are as follows: Objective 1: Efficiently and effectively acquire apple, cold-hardy grape, and tart cherry genetic resources, maintain their safety, genetic integrity, health and viability, and distribute them and associated information worldwide. (usda.gov)
Strains5
- Below we describe eight expert tips on how to handle microbial strains to prevent culture contamination and promote the safety of laboratory personnel. (genengnews.com)
- Obtain microbial strains from an authenticated, trusted source. (genengnews.com)
- Research laboratories run the risk of misidentified or contaminated strains when using microbial cultures obtained from colleagues. (genengnews.com)
- Avoid passaging and subculturing microbial strains too many times. (genengnews.com)
- Identifying novel antimicrobial agents from plant-based compounds may result in anti-microbial drugs effective against the resistant strains. (biomedcentral.com)
Bacteria2
- We're looking to create a desiccation-tolerant strain of bacteria so that we can get full structural capacity while also enhancing microbial viability in super-dry conditions," Srubar said. (insidescience.org)
- However, they appear to have an important role in controlling microbial overgrowth, reducing the number of bacteria in the dental biofilm, decreasing colonization and modifying bacterial metabolism. (bvsalud.org)
Pathogens2
- The fiber optic biosensor can be combined with enrichment broths to determine viability of bacterial pathogens within two hours. (beefresearch.org)
- Cowpea is vulnerable to attack by fungal pathogens at all stages of its growth as well as at storage, thus resulting to low viability, germination, seedling mortality and yield reduction. (scialert.net)
Assays1
- Fluorescence- and absorbance-based microplate viability assays such as MTT, XTT, and resazurin. (biotium.com)
Viable2
- Microbial viability of smoke aerosols based on formazan production and epifluorescent microscopy revealed no significant difference in the viable fraction (~80%) when compared to samples of ambient air. (nature.com)
- From these data, we estimate each fire aerosolized an average of 7 ± 4 × 10 9 cells and 2 ± 1 × 10 8 biological INPs per m 2 burned and conclude that emissions from wildland fire are sources of viable microbial aerosols to the atmosphere. (nature.com)
Contamination2
- Microbial contamination of metalworking fluid s increases the aerosolization of fine particles. (cdc.gov)
- Three different fluid s were tested in this study: pure semisynthetic MWF (without any microbial contamination), P.fluorescens bacterial suspension in water, and P. fluorescens bacterial suspension in semisynthetic MWF. (cdc.gov)
Antibiotic1
- In par- and understanding of AMR through ef- to guide their efforts to curb antibiotic ticular, the viability of achievements fective communication, education and resistance. (who.int)
Carbohydrate1
- These microbial communities reached a higher population density and net carbohydrate fermentation, leading to an increased SCFA production at longer transit times. (biomedcentral.com)
Evaluate2
- We sought to characterise the bacterial and fungal stool flora of preterm neonates and organism viability and evaluate any associations with NEC and sepsis. (bmj.com)
- Serial dilutions were then made to evaluate microbial viability. (bvsalud.org)
Genetic1
- These ubiquitous microbial genetic elements are composed of a protein toxin inhibited by an antitoxin. (lu.se)
Analyses1
- Microbial analyses of foods and biological samples are traditionally carried out by direct plating on agar media. (biomedcentral.com)
Biological1
- Maximizing the potential for successfully developing and deploying a biological control product begins with a carefully crafted microbial screening procedure, proceeds with developing mass production protocols that optimize product quantity and quality, and ends with devising a product formulation that preserves shelf-life, aids product delivery and enhances bioactivity. (usda.gov)
Fungal1
- Investigation revealed that storage materials influence some fungal diseases development as well as the viability of the seed as determined by its germination percentage. (scialert.net)
Growth5
- The term was originally used to describe substances produced by one microorganism that stimulated the growth of others and was later used to describe tissue extracts that stimulated microbial growth and animal feed supplements exerting a beneficial effect on animals by contributing to their intestinal flora balance [ 5 ]. (hindawi.com)
- Some pharmaceutical drugs are formulated with antibiotics, preservatives, or other chemicals that affect microbial viability and growth. (sigmaaldrich.com)
- 3 ] To prevent microbial growth, dentures can be soaked in chemical agents such as chlorhexidine and/or sodium hypochlorite and cleaned with denture cleaners (for instance Polident). (thieme-connect.de)
- Roundup(®) has an inhibitory effect on microbial growth and a microbicide effect at lower concentrations than those recommended in agriculture. (greenmedinfo.com)
- The several practices followed in cowpea storage exploit three factors, normally, temperature, oxygen and mobility, to reduce microbial growth and respiration that result to production of heat leading to rapid disintegration of the tissues of the seeds. (scialert.net)
Ecology1
- The majority of studies in aquatic microbial ecology focus on the separate segments of aquatic ecosystem. (irb.hr)
Probiotics2
- Until recently the most widely used definition which contributed to the development of the probiotic concept in several ways was that of Fuller: "probiotics are live microbial feed supplements which beneficially affect the host animal by improving microbial balance" [ 6 ]. (hindawi.com)
- In fact, we found that commercial microbial products (probiotics) provide a poor solution to this challenge, in particular for key industrial organisms like E. coli . (biorxiv.org)
Diversity1
- Microbial diversity and bioprospecting. (dbkgroup.org)
Additionally1
- Additionally, when handling a microbial culture, work quickly and carefully in an environment that has minimal distractions. (genengnews.com)
Investigate1
- There is a need to investigate the viability of other alternatives for energy generation. (harper-adams.ac.uk)
Temperature4
- Here we analyze how temperature-energy interactions structure sediment microbial communities in two hydrothermally active areas of Guaymas Basin. (nature.com)
- Our results suggest that in hydrothermal sediments temperature determines domain-level dominance, whereas temperature-energy interactions structure microbial communities at the phylum-level and below. (nature.com)
- Evidence suggests that energy-depleted, diffusion-dominated subseafloor environments in many cases have lower microbial temperature maxima (60-80 °C) 4 , 5 than environments with advective supplies of external microbial energy substrates. (nature.com)
- Here we examine the importance of temperature and energy supply (defined as available power) in controlling the microbial abundance and community structure in hydrothermal sediment from two locations in Guaymas Basin, central Gulf of California. (nature.com)
Composition1
- Our group has previously shown that these health benefits are dependent on the microbial composition of the kefir fermentation, and that a pitched kefir (PK1) containing specific traditional microbes can recapitulate the health benefits of a traditional kefir. (frontiersin.org)
Cells2
Variation1
- To assess the variation in physical, chemical and microbial properties when transforming OSR straw into pellets, and the associated advantages and disadvantages. (harper-adams.ac.uk)
Species1
- Strong positive correlation was found between SR and CFU and between CAW and CFU for both microbial species. (bvsalud.org)
Communities2
- Background and aims Evidence suggests that microbial communities in the preterm gut may influence the development of necrotising enterocolitis (NEC) and sepsis. (bmj.com)
- Biofilms are sessile microbial communities growing on surfaces, frequently embedded in a matrix of extracellular polymeric substances (also known as slime). (medscape.com)
Higher2
- oralis and C. albicans on PEEK specimens confirm the impact of the higher surface roughness and contact angle values on the microbial adhesion and describes PEEK as less desirable than ZR and CoCr4 from microbiological aspect. (bvsalud.org)
- In all membrane filtration permeates, microbial concentrations were higher than previously reported for chloraminated membranes, and log10 reduction values were lower than expected. (stanford.edu)
Study1
- The study of microbial dormancy forms the subject of a collaborative project with Prof. Mike Young of UW Aberystwyth and Prof. Arseny S. Kaprelyants and his group at the Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow. (dbkgroup.org)
Community2
Protocols1
- Current Protocols in Cytometry: Volume 11 Microbial Cytometry. (dbkgroup.org)
Applications1
- However, as required for these new applications, maintaining high viability throughout manufacturing, transportation and use remains a significant challenge requiring sophisticated cold-chains and packaging. (biorxiv.org)
Provide1
- Collectively, these studies provide insight into the viability of post‐ruminal NPN supplementation as a strategy to improve fibre digestion and NPN inclusion in the ruminant diet. (trouwnutrition.com)
Selection1
- Microbial selection procedures that require prospective biocontrol agents to possess both efficacy and amenability to production in liquid culture enhance the likelihood of selecting agents with enhanced commercial development potential. (usda.gov)
Results1
- Moreover, these results should be considered in the understanding of the loss of microbiodiversity and microbial concentration observed in raw milk for many years. (greenmedinfo.com)
Water1
- Microbial Water Quality through a Full-Scale Advanced Wastewater Treatment Demonstration Facility. (stanford.edu)