Loading...
  • vivo
  • This inducible transgenic D469del-COMP mouse is the only in vivo model to replicate the critical cellular and clinical features of pseudoachondroplasia. (nih.gov)
  • controls
  • Behavioral analyses demonstrated higher basal locomotor activity of NCAM-EC mice and enhanced responses to amphetamine and (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate compared with wild-type controls. (duke.edu)
  • Growth plates from D469del-COMP mice are narrower than those of controls at P21, 1 month, and 2 months of age. (nih.gov)
  • cell
  • Neural cell adhesion molecule-secreting transgenic mice display abnormalities in GABAergic interneurons and alterations in behavior. (duke.edu)
  • Interneuron cell density was unaltered in the transgenic mice. (duke.edu)
  • Importantly, by crossing the D469del-COMP mouse onto a Chop null background (Ddit3 null), thereby eliminating Chop, the unfolded protein response was disrupted, thus alleviating both D469del-COMP intracellular retention and premature chondrocyte cell death. (nih.gov)
  • The Transgenic Mouse / ES Cell Shared Resource assists investigators in generating, maintaining and storing germline-altered mice. (vanderbilt.edu)
  • This resource, which has been in existence for over 23 years, has generated over 2700 transgenic founder mice from over 750 different DNA constructs and 5000 chimeric mice from over 800 different mouse ES cell clones. (vanderbilt.edu)
  • The Vanderbilt Transgenic Mouse/ES Cell Shared Resource (TMESCSR) provides services, consultation and collaborations to enable the generation, storage and regeneration of genetically altered mice at Vanderbilt. (vanderbilt.edu)
  • We are sorry, but due to low utilization we can no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter ). (vanderbilt.edu)
  • The Transgenic Mouse/ES Cell Shared Resource is supported by NIH grants DK020593 and CA68485. (vanderbilt.edu)
  • gene
  • 1 2 To evaluate the phenotype in more detail, this gene has been introduced in mice, the APOE*3-Leiden mouse. (ahajournals.org)
  • Transgenic mice expressing the human APOE*3-Leiden gene were generated and bred with C57Bl/6J females as described earlier. (ahajournals.org)
  • FOURTH ANNUAL 'GENE TARGETING IN ES CELLS AND TRANSGENIC MICE' WORKSHOP July 28 -- August 9, 1996 CAROLINA WORKSHOPS University of North Carolina at Chapel Hill Students will be introduced to the mouse as a developmental system, and will learn molecular genetic manipulations of this mammal. (bio.net)
  • A seminar series on the application of gene targeting and transgenics in various research areas will run concurrently with the course. (bio.net)
  • Cold Spring Harbor Laboratory, 1994) and Gene Targeting: A Practical Approach by A.L. Joyner, (Oxford University Press, 1993) both provide an excellent introduction to mouse genetics and techniques covered in the course. (bio.net)
  • Another mouse has had a gene altered that is involved in glucose metabolism and runs faster, lives longer, is more sexually active and eats more without getting fat than the average mouse (see Metabolic supermice). (wikipedia.org)
  • vitro
  • Therefore, we have studied different aspects of Btc transgenic female's and male's reproduction, including puberty initiation, ovulation, in vivo and in vitro oocyte maturation, sperm parameters, in vivo and in vitro fertilization, and implantation in order to uncover the reason for their reduced fertility. (uni-muenchen.de)
  • Therefore, we decided to evaluate the in vitro fertilization rate, which turned out to be impaired in the transgenic group. (uni-muenchen.de)
  • The expression pattern at the cellular level, studied by immunohistochemistry, revealed a high expression of Btc in the transgenic cumulus cells, which could be an explanation for the altered in vivo and in vitro fertilization. (uni-muenchen.de)
  • cells
  • Mice transgenic for BAFF have vastly increased numbers of mature B and effector T cells, and develop autoimmune-like manifestations such as the presence of high levels of rheumatoid factors, circulating immune complexes, anti-DNA autoantibodies, and immunoglobulin deposition in the kidneys. (rupress.org)
  • Reprogramming cells within live mice yields a new type of induced pluripotent stem cell. (the-scientist.com)
  • In addition, the direct product of β-secretase cleavage, C99, has toxic or pathogenic effects in cultured cells and in transgenic mice ( 28 , 29 ). (pnas.org)
  • The Takashima mice are remarkable, as they appear to express high levels of the mutant tau protein in only a very few cells in the brain: that is, in some of the pyramidal cells of the hippocampus. (alzforum.org)
  • Because of the very limited number of cells expressing the mutant protein, it appears that the mice will survive (unlike the Lewis/Hutton mice) to advanced age. (alzforum.org)
  • Future studies are needed to clarify whether the altered fertilization is in fact caused by the high expression of Btc in the transgenic cumulus cells. (uni-muenchen.de)
  • In cerebral cortex, Ngb-Tg mice showed increased numbers of neurons ( Fig. 2 a ), astrocytes ( Fig. 2 b ), and endothelial cells (not shown) that constitutively expressed Ngb. (pnas.org)
  • Ngb was also constitutively expressed in Ngb-Tg mouse heart ( Fig. 2 c ), where it was associated most prominently with vascular endothelial cells ( Fig. 2 d ). (pnas.org)
  • Boosting levels of a the immunosuppressive protein PD-L1 in blood stem cells halts diabetes in a mouse model of the disease. (the-scientist.com)
  • Previous attempts to culture PGCs from mice and humans produced embryonic germ cells that look and act like embryonic stem cells. (rxpgnews.com)
  • Embryonic stem cells that recombine with the genomic DNA are selected for and they are then injected into the mice blastocysts. (wikipedia.org)
  • A study in mice has shown that IL-10 is also produced by mast cells, counteracting the inflammatory effect that these cells have at the site of an allergic reaction. (wikipedia.org)
  • Monoclonal antibodies are typically made by cell culture that involves fusing myeloma cells with mouse spleen cells immunized with the desired antigen. (wikipedia.org)
  • found in their study that one-fourth of the nurse cells isolated from mice were double-positives for K5 and K8, while the rest of them were positive only for K8. (wikipedia.org)
  • Embryo
  • Manipulating the Mouse Embryo by B. Hogan, et. (bio.net)
  • In 1974 Rudolf Jaenisch created the first genetically modified animal by inserting a DNA virus into an early-stage mouse embryo and showing that the inserted genes were present in every cell. (wikipedia.org)
  • In 1981 the laboratories of Frank Ruddle from Yale University, Frank Costantini and Elizabeth Lacy from Oxford, and Ralph Brinster and Richard Palmiter in collaboration from the University of Pennsylvania and the University of Washington injected purified DNA into a single-cell mouse embryo utilizing techniques developed by Brinster in the 1960s and 1970s, showing transmission of the genetic material to subsequent generations for the first time. (wikipedia.org)
  • human
  • BAFF was also independently identified as TALL-1 ( 24 ), as THANK, which regulated apoptosis, NF-κB and c-Jun NH 2 -terminal kinase in a human myelocytic cell line ( 25 ), and, more recently, as BlyS, a factor that, when administrated to normal mice, disrupted splenic B and T cell zones and resulted in elevated serum IgM concentrations ( 26 ). (rupress.org)
  • The mice express the V337M mutation of human tau that causes the tauopathy FTDP-17 in addition to wildtype mouse tau. (alzforum.org)
  • As 11-month-old adults, the V337M mice have aggregations of phosphorylated human tau predominantly in neurons of the CA1, CA2, and CA3 areas of hippocampus. (alzforum.org)
  • Genetically modified mice are commonly used for research or as animal models of human diseases, and are also used for research on genes. (wikipedia.org)
  • Genetically modified mice are used extensively in research as models of human disease. (wikipedia.org)
  • Mice are a useful model for genetic manipulation and research, as their tissues and organs are similar to that of a human and they carry virtually all the same genes that operate in humans. (wikipedia.org)
  • Human Monoclonal Antibodies from Transgenic Mice. (wikipedia.org)
  • Alirocumab was discovered by Regeneron Pharmaceuticals using its "VelocImmune" mouse, in which many of the genes coding for antibodies have been replaced with human genes. (wikipedia.org)
  • Human data from patients and controls were used to demonstrate the translation of mouse findings in human disease. (wikipedia.org)
  • A restrictive N-terminal truncation of cTnI occurs at low levels in normal hearts of all vertebrate species examined including human and significantly increases in adaptation to hemodynamic stress and Gsα deficiency-caused failing mouse hearts. (wikipedia.org)
  • cardiac
  • Lack of IL-10 has been shown to cause COX activation and resultant Thromboxane receptor activation to cause vascular endothelial and cardiac dysfunctions in mice. (wikipedia.org)
  • cTnI begins to express in mouse heart at approximately embryonic day 10, and the level gradually increases to one-half of the total amount of TnI in the cardiac muscle at birth. (wikipedia.org)
  • genetic
  • Modeling has been most aggressively pursued in mice, for which the techniques of genetic modification are well developed. (nih.gov)
  • transgene
  • However, some mice survive due to chromosomal rearrangement of proliferating hepatocytes resulting in loss of significant functional components of the transgene. (nih.gov)
  • In these mice, the liver parenchyma is replaced by the transgene deficient hepatocytes within about 8 weeks. (nih.gov)
  • In some Alb-uPA mice there is complete loss of the transgene, and these mice eventually develop hepatocellular adenomas and carcinomas. (nih.gov)
  • However, the mice did not pass the transgene to their offspring, and the impact and applicability of this experiment were, therefore, limited. (wikipedia.org)
  • exhibit
  • The data published so far show that APOE*3-Leiden mice exhibit severe hypercholesterolemia when they are fed a high-fat/high-cholesterol (HFC) diet. (ahajournals.org)
  • onset
  • Mice overexpressing PrP(ΔGPI) developed a late-onset, spontaneous neurologic dysfunction characterized by widespread amyloid deposition in the brain and the presence of a short protease-resistant PrP fragment similar to those found in GSS patients. (nih.gov)
  • In Tg(PrP,ΔGPI) mice, disease onset could be accelerated either by inoculation with brain homogenate prepared from spontaneously ill animals or by coexpression of membrane-anchored, full-length PrP(C). In contrast, coexpression of N-terminally truncated PrP(Δ23-88) did not affect disease progression. (nih.gov)
  • The onset of puberty occurred essentially at the same age in transgenic and non-transgenic females. (uni-muenchen.de)
  • mutant
  • These techniques are the basis for generation and analysis of mutant mice via microinjection and embryonic stem cell manipulation and transfer. (bio.net)
  • neurons
  • Takashima's team also reports weakened neural activity in the hippocampi of their transgenic mice, which they attribute to a smaller number of functional neurons, as well as impaired performance in two rodent behavioral tests. (alzforum.org)
  • females
  • The observation of relatively ineffective matings involving transgenic females (non-productive matings and reduced litter size) during routine breeding led us to functionally evaluate the different stages of the reproductive process. (uni-muenchen.de)
  • The reduced fertility of Btc transgenic females could be attributed to one or more reproductive dysfunctions, such as a decreased ovulation, fertilization or implantation. (uni-muenchen.de)
  • Litter size development showed a significant difference between Btc transgenic females and controls females. (uni-muenchen.de)
  • The implantation of the Btc transgenic mice was delayed, but this was not the reason for the litter size reduction, because the mean number of total embryos either attached or recovered from the uterus of transgenic females was already markedly reduced when compared to the number of embryos present in the uterus of control females. (uni-muenchen.de)
  • We evaluated the number of ovulated oocytes and observed that this parameter did not differ between the two genotypes, however, we observed a statistically significant reduction in the percentage of fertilized oocytes in transgenic as compared to control females, identifying the reason for the reduction in the litter size. (uni-muenchen.de)
  • mutation
  • Other mice expressing the stronger P301L tau mutation of FTDP-17 die too young to allow analysis over time. (alzforum.org)
  • Models
  • A transgenic mouse company is paying researchers who mention its animal models in scientific papers. (the-scientist.com)
  • Since their initial generation in the mid 1990s, transgenic mouse models of Alzheimers's disease (AD) have been proven to be valuable model systems which are indispensable for modern AD research. (hindawi.com)
  • The disease symptoms and potential drugs or treatments can be tested against these mouse models. (wikipedia.org)
  • Sawicki identified therapeutic targets and evaluated the efficacy of many nanoparticle formulations in various cancer mouse models, including for pancreatic cancer, as well as for highly aggressive forms of ovarian cancer for which no effective long-term treatment currently exists. (wikipedia.org)
  • functional
  • Mice lacking functional expression of Fas ( lpr ) or FasL ( gld ) develop severe autoimmune lymphoproliferative disorders, leading to tissue destruction ( 13 ). (rupress.org)
  • amyloid
  • M ) Electron micrograph of PrP amyloid purified from the brain of an ill Tg8015/ Prnp 0/0 mouse at 589 d shows fibrillar aggregates upon negative staining with 2% ammonium molybdate. (nih.gov)
  • As a consequence, the mice can develop amyloid plaques at around 13 months old. (wikipedia.org)
  • genetics
  • Previous experience running this course indicates that both students with and without any prior hands-on experience with tissue culture and mouse genetics will find the course useful. (bio.net)
  • control
  • The timing of nuclear maturation differed between transgenic and control oocytes. (uni-muenchen.de)
  • We engineered transgenic mice that overexpress murine Ngb under the control of a chicken β-actin promoter, resulting in enhanced Ngb expression in multiple cell types and multiple tissues, including brain and heart. (pnas.org)
  • research
  • The Center for the Advancement of Science in Space (CASIS) obtains tissue samples from mice flown to the International Space Station to support valuable commercial muscle wasting research. (nasa.gov)
  • Rodent Research-1 consists of Rodent Research-1 NASA and Rodent Research-1 CASIS and plans to launch 20 mice, including 10 NASA mice, and 10 CASIS mice. (nasa.gov)
  • While that earlier research was done with chimeric chickens, it demonstrated the enormous opportunities that transgenic chickens hold as a therapeutics production system," said Robert Kay, Ph.D. Origen Therapeutics president and chief executive officer. (rxpgnews.com)
  • Great care should be taken when deciding how to use genetically modified mice in research. (wikipedia.org)
  • tissue
  • On-orbit operations require the ability to launch and maintain rodents (mice and rats) in a habitat and in some cases also require rodent euthanasia, dissection, and tissue sample preparation and preservation. (nasa.gov)
  • observation
  • In summary, these mice might enable a more detailed observation of how tau pathology develops over time than was possible before (See also related news item ). (alzforum.org)
  • development
  • Conclusions -This study shows that in APOE*3-Leiden mice, duration of an HFC diet is associated with (1) a craniocaudal progression of lesion development and (2) an increased complexity of atherosclerotic lesions. (ahajournals.org)
  • It is essential for development, and absence of huntingtin is lethal in mice. (wikipedia.org)
  • heart
  • Here we report that Ngb transgenic (Ngb-Tg) mice show reduced sensitivity to ischemia both in brain, where Ngb is normally expressed, and in heart, where it is not. (pnas.org)
  • animals
  • The Transporter and Access Unit are de-stowed and animals are transferred from the Transporter to two Habitats using Mouse Transfer Boxes. (nasa.gov)
  • Before entry into the study, animals were kept on standard mouse chow (SRM-A, Hope Farms). (ahajournals.org)
  • analysis
  • PrP was detected with Fab HuM-P. ( D-L ) Neuropathologic analysis of the hippocampus ( Top ), thalamus ( Middle ), and cerebellum ( Bottom ) from spontaneously ill Tg8015/ Prnp 0/0 mice. (nih.gov)
  • Led by Akihiko Takashima of the RIKEN Brain Science Institute in Saitama, Japan, first author Kentaro Tanemura and colleagues report their analysis of tau transgenic mice that enable the study of tau-induced neurodegeneration in vivo. (alzforum.org)
  • Nature
  • The company made the announcement in conjunction with the publication of an article this week by Origen scientists and a collaborator from the University of California, Davis on its transgenic technology in the journal Nature. (rxpgnews.com)
  • high
  • High levels of hepatic production of uPA lead to hemorrhage and death of many of the mice shortly after birth. (nih.gov)
  • proteins
  • The ability to readily create transgenic chickens through this technology, and then to scale up production through conventional breeding further adds to the practicality of this technology for large-scale production of therapeutic proteins. (rxpgnews.com)
  • technology
  • RxPG] Origen Therapeutics announced today that it has succeeded in developing a robust and versatile technology for genetically modifying chickens that, for the first time, puts avian transgenics on a par with transgenic mice. (rxpgnews.com)
  • system
  • In an investor presentation, Regeneron claimed that with their system, it took only about 19 months from when they first immunized mice with PCSK9 until they filed their IND. (wikipedia.org)