• KOMP
  • The Knockout Mouse Project (KOMP) is a trans-National Institutes of Health (NIH) initiative that aims to generate a comprehensive and public resource comprised of mice containing a null mutation in every gene in the mouse genome. (genome.gov)
  • In developing the NIH KOMP plan, this working group considered the current state of the field and recommendations from members of mouse research community made during a second workshop in March 2005. (genome.gov)
  • To request information or products, researchers can call 1-888-KOMP-MICE or e-mail service@komp.org . (genome.gov)
  • To collect information generated by the KOMP , track progress of the knockout mutant production pipelines, and make the data readily available to all members of the KOMP research network to support, coordinate, and synergize their individual research programs. (genome.gov)
  • Genome Informatics
  • The data will also be exported to other relevant community databases such as Ensembl, the UCSC Genome Browser, NCBI, and Mouse Genome Informatics (MGI). (genome.gov)
  • These data are immediately shared among the scientific and medical research community through a bespoke open access database, and summaries are displayed in other online resources, including the Mouse Genome Informatics database and the Wikipedia-based Gene Wiki. (wikipedia.org)
  • Deficient
  • The SAP-deficient mouse model recapitulates several features of XLP: hyperproliferative T cell response following infections, impaired NK and CD8 cell cytotoxicity, defective humoral immune responses, abnormal germinal center formation, reduction in IgG+ memory B cell numbers, and the absence of NKT and other innate T cells. (taconic.com)
  • C57BL
  • By capitalizing on efficiencies of scale and a centralized production effort, the project intends to make this catalog of mutants available in mouse strain C57BL/6 for two reasons: it is the most widely used strain, and it is the strain for which complete genome sequence has been made available. (genome.gov)
  • For each mutant line, groups of seven male and seven female mice move through a standard analysis pipeline aimed at detecting traits that differ from healthy C57BL/6 mice. (wikipedia.org)
  • laboratory
  • Mice are currently the laboratory animal species most closely related to humans for which the knockout technique can easily be applied. (wikipedia.org)
  • Such mice were found to be 'smarter' than normal mice and were able to handle complex tasks more intelligently compared to 'normal' mice bred in the laboratory. (wikipedia.org)
  • roles
  • Over the past few years, multiple urea transporter knockout mouse models have been generated enabling us to explore the physiological roles of the different urea transporters. (frontiersin.org)
  • normal mice
  • They also found that the mice overfilled their bladders and took much longer to urinate than the normal mice. (redorbit.com)
  • Usually, the new sequence is also given a marker gene, a gene that normal mice don't have and that confers resistance to a certain toxic agent (e.g., neomycin) or that produces an observable change (e.g. colour or fluorescence). (wikipedia.org)
  • mammalian
  • Only a few cell types express Cre recombinase and no mammalian cells express it so there is no risk of accidental activation of lox sites when using conditional gene knockout in mammals. (wikipedia.org)
  • Disorders
  • Gene targeting has already produced more than five hundred different mouse models of human disorders, including cardiovascular and neuro-degenerative diseases, diabetes and cancer. (scienceblogs.com)
  • metabolic
  • Alcohol and aldehyde dehydrogenases: retinoid metabolic effects in mouse knockout models. (nih.gov)
  • Elucidation of the vitamin A metabolic pathway and investigation of the endogenous function of vitamin A metabolites has been greatly improved by development of mouse ADH, RDH, and RALDH loss-of-function models. (nih.gov)
  • Another mouse has had a gene altered that is involved in glucose metabolism and runs faster, lives longer, is more sexually active and eats more without getting fat than the average mouse (see Metabolic supermice). (wikipedia.org)
  • models
  • These mouse models serve as instrumental tools for providing new insight into retinoid function. (nih.gov)
  • Knockout animal models also have provided a platform on which to develop and test novel drug therapies. (britannica.com)
  • Furthermore, knocking out a gene may not produce any phenotypic change, and the changes observed in mouse models may be quite different from those observed in humans when the same gene is inactivated in both species. (britannica.com)
  • Many mouse models are named after the gene that has been inactivated. (wikipedia.org)
  • Other mouse models are named according to their physical characteristics or behaviours. (wikipedia.org)
  • Genetically modified mice are used extensively in research as models of human disease. (wikipedia.org)
  • The disease symptoms and potential drugs or treatments can be tested against these mouse models. (wikipedia.org)
  • Multiple studies have been done using mouse knockout models to determine IRG function. (wikipedia.org)
  • abnormalities
  • Since UT-B has a wide tissue distribution, multiple phenotypic abnormalities have been found in UT-B null mice, such as defective urine concentration, exacerbated heart blockage with aging, depression-like behavior, and earlier male sexual maturation. (frontiersin.org)
  • sequences
  • Artificial DNA sequences typically are introduced into mouse ES cells using a retrovirus or other viral vector, and the modified ES cells are then grown in cell cultures . (britannica.com)
  • studies
  • This review summarizes the new insights of urea transporter functions in different organs, gleaned from studies of urea transporter knockout mice, and explores some of the potential pharmacological prospects of urea transporters. (frontiersin.org)
  • Mouse knockout studies show that PDGF-C is required for palatogenesis. (wikipedia.org)
  • Studies in mice have characterized the importance of the type 2 effector molecule IFNγ in various cell types and gone on to determine the importance of these proteins in intracellular pathogen resistance. (wikipedia.org)
  • Dependency on the IRGs is best exemplified in mouse studies. (wikipedia.org)
  • human
  • Considering the high concentration of urea in human (285 mmol/L), rat (700 mmol/L), and mouse (1800 mmol/L) urine, the amount of urea in the urine should inevitably cause osmotic diuresis in the renal collecting ducts. (frontiersin.org)
  • The SAP Knockout mouse was generated in 2001 by Michael J. Czar and Pamela Schwartzberg of the National Human Genome Research Institute, NIH. (taconic.com)
  • protein
  • Both groups of mice had normal appearing bladders, but the group without the integrin protein had very little bladder control. (redorbit.com)
  • The mouse will likely produce the protein from the wild-type copy of the gene, but depending on how the gene is regulated it is likely that expression of the protein will be below wild-type levels. (biology-online.org)
  • If there is some of the protein made, the mouse is a knock-down and not a knock-out. (biology-online.org)
  • SAP protein is not present in these mice. (taconic.com)
  • For example, the p53 knockout mouse is named after the p53 gene which codes for a protein that normally suppresses the growth of tumours by arresting cell division and/or inducing apoptosis. (wikipedia.org)
  • study
  • In the present study, we therefore compared blood pressure variability in knockout mice that lack specifically the gene for endothelial nitric oxide synthase with their respective wild-type controls. (ahajournals.org)
  • generations
  • In 1981 the laboratories of Frank Ruddle from Yale University, Frank Costantini and Elizabeth Lacy from Oxford, and Ralph Brinster and Richard Palmiter in collaboration from the University of Pennsylvania and the University of Washington injected purified DNA into a single-cell mouse embryo utilizing techniques developed by Brinster in the 1960s and 1970s, showing transmission of the genetic material to subsequent generations for the first time. (wikipedia.org)
  • research
  • The research team tested two groups of mice. (redorbit.com)
  • The University of California, Davis (UC Davis) and Children's Hospital Oakland Research Institute (CHORI) in Oakland, Calif., will collaborate to preserve, protect and make available knockout mice and related products available to the research community. (genome.gov)
  • Examples of research in which knockout mice have been useful include studying and modeling different kinds of cancer, obesity, heart disease, diabetes, arthritis, substance abuse, anxiety, aging and Parkinson's disease. (wikipedia.org)
  • Great care should be taken when deciding how to use genetically modified mice in research. (wikipedia.org)
  • animals
  • Regardless, this 52% increase can be almost solely attributed to the increasing use of knockout and knock-in animals (see the earlier post for details). (scienceblogs.com)