Metaphase. Medical search. Frequent questions

Metaphase (from Ancient Greek μετα- (meta-) beyond, above, transcending and from Ancient Greek φάσις (phásis) 'appearance') is a stage of mitosis in the eukaryotic cell cycle in which chromosomes are at their second-most condensed and coiled stage (they are at their most condensed in anaphase). (wikipedia.org)
Metaphase (from the Greek μετά, "adjacent" and φάσις, "stage") is a stage of mitosis in the eukaryotic cell cycle in which chromosomes are at their second-most condensed and coiled stage (they are at their most condensed in anaphase). (askdifference.com)
The stage of mitosis and meiosis, following prophase and preceding anaphase, during which the chromosomes are aligned along the metaphase plate. (askdifference.com)
A deconvolved wide-field fluorescence microscope image of human HeLa cancer cells in metaphase of mitosis. (mitosispictures.com)
Metaphase: What Happens in this Stage of Mitosis & Meiosis? (sciencing.com)
Metaphase is one of the stages of mitosis which is characterized by the appearance of chromosomes due to condensation of duplicated genetic material (chromatin/genomic DNA). (laboratorynotes.com)
Mitosis includes prophase, prometaphase, metaphase, and anaphase, as well as telophase, during which chromosome copies are carefully separated in preparation for cytokinesis, where the cytoplasm divides. (coursehero.com)

The HANABI-PII Plus Metaphase Chromosome Harvester is designed for today's cytogenetic laboratories that require increased productivity and throughput - plus it delivers the consistent, professional quality you demand every time. (adsbiotec.com)

At the end of metaphase, chromosomes are aligned at the metaphase plate. (laboratorynotes.com)
The chromosomes have aligned at the metaphase plate. (cikgunaza.com)

Chromosomes are normally visible under a light microscope only during the metaphase of cell division (where all chromosomes are aligned in the center of the cell in their condensed form). (wikipedia.org)

Karyokinesis can further be divided into prophase, metaphase, anaphase, and telophase for thorough understanding, though it is a constant process. (guyhowto.com)

The centromeres of the chromosomes convene themselves on the metaphase plate (or equatorial plate), an imaginary line that is equidistant from the two centrosome poles. (wikipedia.org)
As microtubules find and attach to kinetochores in prometaphase, the centromeres of the chromosomes convene along the metaphase plate or equatorial plane, an imaginary line that is equidistant from the two centrosome poles. (cikgunaza.com)

Prophase vs. Metaphase - What's the Difference? (askdifference.com)
Primary spermatocytes are the male germ cells before meiosis I. To examine whether these 4n diploid cells are genetically competent to fertilize oocytes and support full embryo development, we introduced the nuclei of pachytene/diplotene spermatocytes into oocytes that were arrested in prophase I (germinal vesicle stage), metaphase I, or metaphase II (Met II). (elsevierpure.com)

First, imagine this is the situation in metaphase I. All three maternal chromosomes are attached to the spindle nearer to the lower pole while the three paternal chromosomes are attached to the spindle nearer to the upper pole. (homeworkclinic.com)
A previous study showed that pretreatment of mouse oocytes with 3% (0.09 M) sucrose allowed visualization of the metaphase spindle and chromosomes under standard light microscopy and led to a 100% enucleation rate. (ox.ac.uk)
The kinetochore fibers of the spindle connect to the kinetochore region at the centromere of the chromosome and align them at the equator of the spindle forming an equatorial plate or metaphase plate. (guyhowto.com)
The alignment of chromosomes at the metaphase plate (spindle equator), a plane halfway between the poles of the spindle. (planteome.org)
Colcemid, an inhibitor of the mitotic spindle, inhibits the formation of mitotic spindles, thus stopping the segregation of chromosomes and cells remain in the metaphase. (laboratorynotes.com)
Tension from spindle fibers aligns chromosomes at the metaphase plate. (cellsalive.com)

Cryo-electron tomography and small-angle X-ray scattering were used to investigate the chromatin folding in metaphase chromosomes. (unibas.ch)

Hypertonic medium treatment for localization of nuclear material in bovine metaphase II oocytes. (ox.ac.uk)
Oocytes enucleated at the second metaphase stage (MII) are often used as recipient cytoplasts for nuclear transfer. (ox.ac.uk)
Staining of DNA with Hoechst 33342 revealed that these projections coincided with the position of the metaphase chromosomes in 100% of sucrose-treated oocytes, whereas only 31% of oocytes showed alignment of the position of Pb1 with their nuclear materials. (ox.ac.uk)
This study demonstrated that 0.3 M sucrose treatment of bovine oocytes facilitates the localization of metaphase chromosomes under normal light microscopy and hence increases enucleation efficiency without compromising the in vitro development potential of cloned embryos by nuclear transfer. (ox.ac.uk)
In this study, we investigated the role of Cdc25B during metaphase II (MII) arrest in mouse oocytes. (molcells.org)
After activation of the Met II oocytes that were produced, those microfertilized at metaphase I showed the best developmental ability in vitro, and three of these embryos developed into full-term offspring after embryo transfer. (elsevierpure.com)

Chromosomal ultrastructure and ribonucleoproteins (RNP) at mitotic metaphase in garlic (Allium sativum L. ) were studied. (jipb.net)

B: First meiotic metaphase. (cdc.gov)
E: First meiotic metaphase of the same insect shown in 2D. (cdc.gov)

The analysis of metaphase chromosomes is one of the main tools of classical cytogenetics and cancer studies. (wikipedia.org)
Preparation of metaphase chromosome spread is an important technique in cytogenetics. (laboratorynotes.com)

citation needed] In metaphase, microtubules from both duplicated centrosomes on opposite poles of the cell have completed attachment to kinetochores on condensed chromosomes. (wikipedia.org)

In metaphase chromosome is a duplicated structure having two sister chromatids, connected at a point called centromere or primary constriction . (guyhowto.com)
Intensity Integrated Laplacian Based Thickness Measurement for Detecting Human Metaphase Chromosome Centromere Location. (cytognomix.com)
Accurate detection of the human metaphase chromosome centromere is an important step in many chromosome analysis and medical diagnosis algorithms. (cytognomix.com)

In certain types of cells, chromosomes do not line up at the metaphase plate and instead move back and forth between the poles randomly, only roughly lining up along the midline. (cikgunaza.com)

Three chromosome-specific repetitive probes labeled with either amino acetyl fluorene (AAF), mercury, or biotin were hybridized simultaneously to metaphase chromosomes prepared from human blood lymphocytes or to interphase tumor nuclei. (nih.gov)

Cohesin holds the sister CHROMATIDS together during METAPHASE and its cleavage results in chromosome segregation. (bvsalud.org)

There's no better place to meet thought leaders," declares speaker Bryce Rutter, PhD, IDSA, of Metaphase in a new video. (core77.com)

It shows the metaphase plate just about to separate the chromosomes. (mitosispictures.com)
In metaphase, chromosomes align at the metaphase plate. (guyhowto.com)
Link to all annotated objects annotated to metaphase plate congression. (planteome.org)
Link to all direct and indirect annotations to metaphase plate congression. (planteome.org)
All chromosomes (blue) but one have arrived at the metaphase plate. (cikgunaza.com)
The metaphase plate depicts strong speckled pattern with some coarse speckles standing out. (cdc.gov)

With twice the brightness, Metaphase Technologies LED Backlights offer a great alternative to Cold Cathode Fluorescent illumination. (edmundoptics.com)

Normal metaphase spreads are used in methods like FISH and as a hybridization matrix for comparative genomic hybridization (CGH) experiments. (wikipedia.org)

For classical cytogenetic analyses, cells are grown in short term culture and arrested in metaphase using mitotic inhibitor. (wikipedia.org)
Malignant cells from solid tumors or leukemia samples can also be used for cytogenetic analysis to generate metaphase preparations. (wikipedia.org)
The HANABI-PII Plus benchmark technology fully automates the multi-step process of preparing and harvesting metaphase cells derived from blood and bone marrow cell cultures and from other cell suspension cultures for cytogenetic analysis. (adsbiotec.com)

Inspection of the stained metaphase chromosomes allows the determination of numerical and structural changes in the tumor cell genome, for example, losses of chromosomal segments or translocations, which may lead to chimeric oncogenes, such as bcr-abl in chronic myelogenous leukemia. (wikipedia.org)

Cells burst when they hit the slide surface, resulting in the release of chromosomes due to lack of nuclear membrane in metaphase cells. (laboratorynotes.com)

For more than two decades, Metaphase Technologies has been developing products that advance "The Quality of Light" through engineering and manufacture of uniform diffuse high brightness and ultra brightness LED illuminators for machine vision, military, and specialty lighting applications. (saber1.com)

Here, we have applied infrared nanospectroscopy (AFM-IR) to demonstrate molecular difference between eu- and heterochromatin and generate infrared maps of single metaphase chromosomes revealing detailed information on their molecular composition, with nanometric lateral spatial resolution. (monash.edu)

Metaphase chromosome spread is used for karyotyping including analysis of numerical or structural changes in chromosomes. (laboratorynotes.com)

Metaphase Technologies Inc. will have its new dark field line light called the "Eclipser" on display at Automate 2015. (vision-systems.com)
Metaphase Technologies Inc. (vision-systems.com)
First, to standardize built-in constant current drivers, Metaphase continues to synergize cutting-edge machine vision LED lighting & control technologies in an effort to simplify innovation and increase customer return on investment. (saber1.com)

MetaPhase Consulting was named a 2023 Elev8 GovCon Honoree by Orange Slices AI. (metaphaseconsulting.com)
MetaPhase Consulting won SECAF's 2022 Government Contractor of the Year Award in the $27.5M - $50M in revenue category. (metaphaseconsulting.com)
As the CEO at MetaPhase Consulting, 弗雷德负责通过"敬业团队"来实现公司的目标, Meaningful Work (faceoff-6.com)
Brett McLaren is the Chief Strategy Officer of MetaPhase Consulting. (faceoff-6.com)
Prior to MetaPhase, Andy was a Senior Director at Eagle Hill Consulting, Vice President at ICF International, and Managing Consultant at IBM. (faceoff-6.com)
Danielle Toole is a Vice President at MetaPhase Consulting. (faceoff-6.com)

These metaphase cells are lysed in a controlled manner on a slide that causes the release and spread of chromosomes from individual cells that remain close to each other, thus making it possible to analyze chromosomes from individual cells. (laboratorynotes.com)
To lyse metaphase cells in a controlled manner, cells are treated with a hypotonic solution that results in an increase in the volume of cells and cells become prone to lyse upon mechanical stress. (laboratorynotes.com)
MDI-GSH also increased the number of cells in metaphase. (cdc.gov)

This is a cell during metaphase I. For each pair of chromosomes, any gamete produced by this cell could contain either the maternal chromosome or the paternal chromosome. (homeworkclinic.com)

Here is a second possible arrangement of the chromosomes at metaphase. (homeworkclinic.com)

Metaphase spread of normal human male chromosomes, showing Y-banding. (wellcomecollection.org)

The proposed method was observed to be more accurate and statistically significant compared to a centerline based method when tested with 226 human metaphase chromosomes. (cytognomix.com)

Methods available in literature yield unreliable results mainly due to high variability of morphology in metaphase chromosomes and boundary noise present in the image. (cytognomix.com)

However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. (monash.edu)

Metaphase has extensive expertise in engineering flexible lighting solutions for ease of integration into vision systems design. (saber1.com)

MetaPhase works at the intersection of business-to-IT partnerships-creating, deploying, and supporting practical solutions that serve as a force multiplier for government. (metaphaseconsulting.com)

Metaphase is the third of the five phases of biological cell division, or more specifically, the division of what is inside that cell's nucleus. (sciencing.com)

and fluorescent in, situ hybridization (FISH) to metaphase chromosomes revealed an insertion of part of chromosome 16 on chromosome 11. (lu.se)

During metaphase the X-shaped structure is called a metaphase chromosome, which is highly condensed and thus easiest to distinguish and study. (wikipedia.org)

Each of these in turn includes its own metaphase, appropriately named metaphase I and metaphase II. (sciencing.com)

This is a general protocol for the preparation of metaphase chromosomes spread from any adherent cell lines. (laboratorynotes.com)

Chromosomes are condensed (thickened) and highly coiled in metaphase, which makes them most suitable for visual analysis. (wikipedia.org)

Anatomy35

Organisms8

Diseases8

Chemicals and Drugs33

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes49

Disciplines and Occupations1

Information Science1

Mitosis7

HANABI-PII Plus Metaphase Chromosome Harvester1

Aligned at the metaphase plate2

Chromosomes are aligned1

Anaphase1

Centromeres of the chromosomes convene2

Prophase2

Spindle6

Chromatin1

Oocytes6

Mitotic metaphase1

Meiotic2

Cytogenetics2

Opposite poles of the cell1

Centromere3

Poles1

Interphase1

Chromosome segregation1

Bryce Rutter1

Plate6

Backlights1

Spreads1

Cytogenetic3

Chromosomal1

Nuclear1

Brightness1

Generate1

Structural changes1

Technologies3

Consulting6

Cells3

Gamete1

Arrangement1

Normal1

Human1

Results1

Molecular1

Vision Systems Design1

Solutions1

Cell Division1

FISH1

Structure1

Includes1

Preparation1

Highly1