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  • function
  • Background: Increasing evidences indicated that adipose-derived mesenchymal stem cells (ADMSCs) can stay survive, then gradually proliferate and differentiate into myocardial cells after transplanted into damaged areas and improve function of heart.Methods: In this article, ADMSCs were isolated from adipose tissue of Wistar rats and cultured. (biomedsearch.com)
  • When treated with 5-azacytidine (5-aza), ADMSCs were differentiated into myocardial cells, then we implant these cells into myocardium of rats of DCM to observe cell population and differentiation and compare cardiac function and hemodynamics changes before and after transplantation.Results: The expression of Cardiac-specific markers indicated that ADMSCs which were isolated from adipose tissue of Wistar rats can differentiate into various cell types. (biomedsearch.com)
  • MSCs can fully suppress T cell function which involves some degree of MSC activation or 'licensing' thought to involve interferon (IFN)-γ in conjunction with IL-1α, IL-1β or tumour necrosis factor-a. (comparetrials.com)
  • various
  • Recently, significant interactions between MSCs and cells from the immune system have been demonstrated:MSCs were found to downregulate T and B lymphocytes, natural killer cells (NK) and antigen presenting cells through various mechanisms, including cell-to-cell interaction and soluble factor production. (comparetrials.com)
  • regenerative
  • Finally it is aimed to establish a new and regenerative treatment protocol alternative to liver transplantation. (clinicaltrials.gov)
  • Several different forms of stem cells have been identified and studied in the field of regenerative medicine. (wikipedia.org)
  • Therefore, an ideal treatment must possess both immunomodulating properties to control irregular autoimmune responses to prevent further damage and regenerative properties to stimulate natural repair mechanisms and replace damaged cells. (wikipedia.org)
  • Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke. (wikipedia.org)
  • Marrow nucleated cells are used every day in regenerative orthopedics. (wikipedia.org)
  • myocardial infarction
  • The study was designed to investigate whether ultrasound-mediated microbubble destruction can enhance the transplantation of bone marrow mesenchymal stem cells (BMSCs) for myocardial infarction therapy, twenty myocardial infarction pigs were assigned into the ultrasound microbubble destruction group (n=12, Sonovue were infused into coronary artery with ultrasonic radiation, then BMSCs were injected into infarction region) and the cell control group (n=8, only subjected to BMSCs treatment). (omicsonline.org)
  • Our study first time testifies in pig models that ultrasound-mediated microbubble destruction promotes BMSC transplantation for myocardial infarction therapy. (omicsonline.org)
  • Myocardial infarction is caused by the loss of myocardial cells and decreased cardiac systolic and diastolic functions, and inevitably leads to heart failure. (omicsonline.org)
  • An example of this is the use of cells to replace cardiomyocytes after myocardial infarction. (wikipedia.org)
  • Among several clinical trials that have reported that adult stem-cell therapy is safe and effective, powerful effects have been reported from only a few laboratories, infarcts as well as heart failure not arising from myocardial infarction. (wikipedia.org)
  • Stem-cell therapy for treatment of myocardial infarction usually makes use of autologous bone-marrow stem cells (a specific type or all), however other types of adult stem cells may be used, such as adipose-derived stem cells. (wikipedia.org)
  • tissues
  • Mesenchymal stem cells have the capacity to become any type of fully developed cell, which can contribute to replacing muscle tissues or internal organs. (wikipedia.org)
  • Pluripotent stem cells can be directly obtained from normal human mesenchymal tissues without using artificial manipulations such as gene introduction. (wikipedia.org)
  • As a result, that leaves the very small number of MSC's in the marrow as cells capable of differentiating into tissues of interest to joint preservation. (wikipedia.org)
  • The term encompasses multipotent cells derived from other non-marrow tissues, such as placenta, umbilical cord blood, adipose tissue, adult muscle, corneal stroma or the dental pulp of deciduous baby teeth. (wikipedia.org)
  • tissue
  • By this way, non-immunogeneic mesenchymal stem cells which have healthy genetic structure, can manufacture the required enzyme, will be repopulated in the damaged tissue and contribute to the clinical improvement. (clinicaltrials.gov)
  • In this special topic, different views on the mechanisms of immunomodulation and regeneration by MSC and on the possible applications of MSC in transplantation and tissue regeneration will be put together. (frontiersin.org)
  • Stem cells (SC) are the foundation cells for every organ, tissue and cell in the body. (clinicaltrials.gov)
  • Replenish new functional cells through spontaneous differentiation into tissue-compatible cells. (wikipedia.org)
  • Muse cells are shown to home into the damage site and spontaneously differentiate into tissue-compatible cells according to the microenvironment to contribute to tissue regeneration when infused into the blood stream. (wikipedia.org)
  • These cells then integrate into the site of injury, replacing damaged tissue, and thus facilitate improved function of the organ or tissue. (wikipedia.org)
  • As a result, many researchers have focused on adult stem cells , or stem cells isolated from adult humans that can be transplanted into damaged tissue. (wikipedia.org)
  • At some point, a signal is introduced (either in culture or after transplant to the damaged tissue) for the cells to differentiate into the end tissue (in this discussion, cartilage). (wikipedia.org)
  • CordLabs licenses its proprietary cord lining stem cell storage technology to cord blood banks, allowing them to also bank cord tissue from which the cord lining stem cells are derived. (wikipedia.org)
  • A similar study combining the cord lining MSC with carrier endothelial cells within a fibrin matrix in-vivo also revealed improvement in cardiac function, reduction in scar tissue formation and better vascularization. (wikipedia.org)
  • Mesenchymal stem cell population isolated from the subepithelial layer of umbilical cord tissue. (wikipedia.org)
  • However, the implanted biological pacemaker cells still typically need to be supplemented with an artificial pacemaker while the cells form the necessary electrical connections with cardiac tissue. (wikipedia.org)
  • N. K. Venkataramana is an Indian neurosurgeon and the founder of ANSA Research Foundation, a non profit non governmental organization promoting research on neuroscience, neurological disorders, cancer biology, stem cells and tumor tissue repository. (wikipedia.org)
  • The proposed method includes the conversion of mesenchymal stem cells (cells usually intended for generation of bones and adipose tissue) into neurons, after a short exposure to retinoic acid diluted in ethanol. (wikipedia.org)
  • citation needed] Possible mechanisms of recovery include: Generation of heart muscle cells Stimulation of growth of new blood vessels to repopulate damaged heart tissue Secretion of growth factors Assistance via some other mechanism It may be possible to have adult bone-marrow cells differentiate into heart muscle cells. (wikipedia.org)
  • differentiate
  • There are cells in the human body that are capable to renew themselves and differentiate to a diverse range of specialized cell types. (clinicaltrials.gov)
  • In many laboratory studies, it is observed that human bone marrow derived mesenchymal stem cells, transplanted to animals with induced liver damage, differentiate into the albumin producing hepatocytes without fusion. (clinicaltrials.gov)
  • They can differentiate into many cell types including bone, fat, and muscle which allow them to treat a large range of disorders. (wikipedia.org)
  • In this paradigm multipotent or unipotent cells differentiate into a specific cell type in the lab or after reaching the site of injury (via local or systemic administration). (wikipedia.org)
  • Most of these cells are CD34+ heme progenitors (destined to differentiate into blood components), while very few are actually MSC's capable of differentiating into bone, cartilage, or muscle. (wikipedia.org)
  • They can be extracted, cultured, and induced to differentiate into mature cell types such as neurons. (wikipedia.org)
  • feasibility and safety
  • Researchers at the Cleveland Clinic, University Hospitals Seidman Cancer Center, and Case Western Reserve University are collaborating on a ground-breaking study that will test the feasibility and safety of using the body's own stem cells to treat MS. (healthcanal.com)
  • patient's
  • It is aimed to enable liver regeneration, decrease fibrosis rate, improve patient's health conditions, increase ceruloplasmin synthesis, ameliorate disorder of copper metabolism, decrease the need for chelating agents, increase living standards of patients, and prolong waiting time for liver transplantation. (clinicaltrials.gov)
  • In MSC therapy the cells are extracted from the adult patient's bone marrow via a procedure called bone marrow aspiration. (wikipedia.org)
  • Stromagen is a product that is made of stem cells taken from a patient's bone marrow and grown in the laboratory. (wikipedia.org)
  • After a patient's bone marrow is destroyed by treatment with whole body irradiation or chemotherapy, these cells are injected back into the patient to help rebuild bone marrow. (wikipedia.org)
  • Genetic
  • Animal studies on cord lining EpSC have shown that genetic modifications using the proinsulin gene allowed transplanted stem cells to lower blood glucose levels in diabetic animals. (wikipedia.org)
  • These cryovials are then placed into a tray, labeled with the cell line's genetic data, and placed into cryogenic freezers. (wikipedia.org)
  • Graft
  • Stromagen has been studied in the prevention of graft-versus-host disease during stem cell transplant in patients receiving treatment for cancer. (wikipedia.org)
  • Another stem-cell therapy called Prochymal, was conditionally approved in Canada in 2012 for the management of acute graft-vs-host disease in children who are unresponsive to steroids. (wikipedia.org)
  • Biological
  • Generally speaking, a biological pacemaker is one or more types of cellular components that, when "implanted or injected into certain regions of the heart," produce specific electrical stimuli that mimic that of the body's natural pacemaker cells. (wikipedia.org)
  • The first successful experiment with biological pacemakers was carried out by Arjang Ruhparwar's group at Hannover Medical School in Germany using transplanted fetal heart muscle cells. (wikipedia.org)
  • When they are no longer able to perform their purpose, interference of new cells and biological cues is provided by a scaffold material. (wikipedia.org)
  • neural
  • born February 1970) is the chairmen of Stem Cell Transplantation Center (also called as Department of Neural Stem Cells) in the General Hospital of Chinese People's Armed Police Forces. (wikipedia.org)
  • Pharmacological activation of endogenous neural stem cells has been reported to induce neuroprotection and behavioral recovery in adult rat models of neurological disorder. (wikipedia.org)
  • neurons
  • The standard test to confirm multipotency is differentiation of the cells into osteoblasts, adipocytes and chondrocytes as well as myocytes and neurons. (wikipedia.org)
  • Stroke and traumatic brain injury lead to cell death, characterized by a loss of neurons and oligodendrocytes within the brain. (wikipedia.org)
  • lymphocytes
  • Immunological analysis revealed an increase in the proportion of CD4 + CD25 + regulatory T cells, a decrease in the proliferative responses of lymphocytes, and the expression of CD40 + , CD83 + , CD86 + , and HLA-DR on myeloid dendritic cells at 24 hours after MSC transplantation. (pubmedcentralcanada.ca)
  • In patients with MS, autoreactive lymphocytes such as T and B cells cause damage to the CNS by attacking myelin proteins. (wikipedia.org)
  • therapy
  • Till the end of 2013 he has treated over 7,000 patients with stem cell therapy with his medical team, involving many diseases, such as cerebral palsy, spinal cord injury, traumatic brain injury, diabetes, ulcerative colitis and cirrhosis. (wikipedia.org)
  • Cell therapy originated in the nineteenth century when scientists experimented by injecting animal material in an attempt to prevent and treat illness. (wikipedia.org)
  • Today two distinct categories of cell therapy are recognized. (wikipedia.org)
  • The first category is cell therapy in mainstream medicine. (wikipedia.org)
  • Cell therapy can be defined as therapy in which cellular material is injected into a patient. (wikipedia.org)
  • The origins of cell therapy can perhaps be traced to the nineteenth century, when Charles-Édouard Brown-Séquard (1817-1894) injected animal testicle extracts in an attempt to stop the effects of aging. (wikipedia.org)
  • In 1931 Paul Niehans (1882-1971) - who has been called the inventor of cell therapy - attempted to cure a patient by injecting material from calf embryos. (wikipedia.org)
  • Bone marrow transplants have been found to be effective, along with some other kinds of human cell therapy - for example in treating damaged knee cartilage. (wikipedia.org)
  • In recent times, cell therapy using human material has been recognized as an important field in the treatment of human disease. (wikipedia.org)
  • In mainstream medicine, cell therapy is supported by a distinct healthcare industry which sees strong prospects for future growth. (wikipedia.org)
  • Cell therapy is targeted at many clinical indications in multiple organs and by several modes of cell delivery. (wikipedia.org)
  • He is known to have introduced microdialysis of brain and stem cell therapy for cerebral palsy for the first time in Asia. (wikipedia.org)
  • The therapy consists in removing cells from the bone marrow of patients, their in vitro manipulation (incubation of stem cells for 2 hours in an 18 micromolar solution of retinoic acid), and finally their infusion into patients themselves. (wikipedia.org)
  • Stem-cell therapy is the use of stem cells to treat or prevent a disease or condition. (wikipedia.org)
  • this is the only form of stem-cell therapy that is widely practiced. (wikipedia.org)
  • regeneration
  • As the number of MSC's that can be isolated from bone marrow is fairly limited, most research in cartilage regeneration has focused on the use of culture expanded cells. (wikipedia.org)
  • In order to reduce the length of time for regeneration, further research is being conducted to speed regeneration such as nerve grafts and stem cells. (wikipedia.org)
  • function
  • Background: Increasing evidences indicated that adipose-derived mesenchymal stem cells (ADMSCs) can stay survive, then gradually proliferate and differentiate into myocardial cells after transplanted into damaged areas and improve function of heart.Methods: In this article, ADMSCs were isolated from adipose tissue of Wistar rats and cultured. (biomedsearch.com)
  • When treated with 5-azacytidine (5-aza), ADMSCs were differentiated into myocardial cells, then we implant these cells into myocardium of rats of DCM to observe cell population and differentiation and compare cardiac function and hemodynamics changes before and after transplantation.Results: The expression of Cardiac-specific markers indicated that ADMSCs which were isolated from adipose tissue of Wistar rats can differentiate into various cell types. (biomedsearch.com)
  • MSCs can fully suppress T cell function which involves some degree of MSC activation or 'licensing' thought to involve interferon (IFN)-γ in conjunction with IL-1α, IL-1β or tumour necrosis factor-a. (comparetrials.com)
  • various
  • Recently, significant interactions between MSCs and cells from the immune system have been demonstrated:MSCs were found to downregulate T and B lymphocytes, natural killer cells (NK) and antigen presenting cells through various mechanisms, including cell-to-cell interaction and soluble factor production. (comparetrials.com)
  • tissue
  • Amniotic fluid-derived mesenchymal stem cells lead to bone differentiation when cocultured with dental pulp stem cells," Tissue Engineering-Part A , vol. 17, no. 5-6, pp. 645-653, 2011. (hindawi.com)
  • Human amniotic fluid stem cells seeded in fibroin scaffold produce in vivo mineralized matrix," Tissue Engineering-Part A , vol. 17, no. 21-22, pp. 2833-2843, 2011. (hindawi.com)
  • The methodology to create induced pluripotent stem cells (iPSCs) affords the opportunity to generate cells specific to the individual providing the host tissue. (jove.com)
  • Additionally, these cells are a type of adult stem cell (found in both children and adults), unspecialized cells that can develop into tissue- or organ-specific cells. (newswise.com)
  • In adult patients, MSCs in the heart have been shown to reduce scar tissue, reduce inflammation, cause new small vessels to grow, and stimulate the heart to regenerate itself, causing heart muscle cells and cardiac stem cells to grow. (newswise.com)
  • Orthotopic models have their limitations too, but with proper technique tumor cells of interest can be accurately engrafted into tissue that most closely mimics conditions in the human brain. (jove.com)
  • PTH/SDF-1a cotherapy induces CD90+CD34- stromal cells migration and promotes tissue regeneration in a rat periodontal defect model. (abcam.co.jp)
  • Tissue necrosis is generally due to the cells being subjected to physical trauma or to a chemical poison, for example. (google.com)
  • This drives efforts to develop ways of using a person's own cells to grow, or re-grow cartilage tissue to replace missing or damaged cartilage. (wikipedia.org)
  • These cells are held in place by a small piece of soft tissue from the tibia, called a periosteal flap, which is sutured over the area to serve as a watertight lid. (wikipedia.org)
  • citation needed] A variation on the Carticel technique, called matrix-associated autologous chondrocyte transplantation (MACT), grows the patient's cells in a 3D matrix of resorbable tissue which is implanted via an open or arthroscopic procedure. (wikipedia.org)
  • The cells grow in self-organized spheroid matrices which are implanted via injected fluid or inserted tissue matrix. (wikipedia.org)
  • Research into new treatments for spinal cord injuries includes stem cell implantation, engineered materials for tissue support, epidural spinal stimulation, and wearable robotic exoskeletons. (wikipedia.org)
  • The damage can also be divided into primary and secondary injury: the cell death that occurs immediately in the original injury, and biochemical cascades that are initiated by the original insult and cause further tissue damage. (wikipedia.org)
  • Adult mesenchymal stem cells (MSCs) are being used by researchers in the fields of regenerative medicine and tissue engineering, to artificially reconstruct human tissue which has been previously damaged. (wikipedia.org)
  • The procedure involves removing adult stem cells of fatty tissue from lower body, and refining it to be able to re-inject living adipose stem cells into specific areas of the face without incision. (wikipedia.org)
  • The FAMI procedure differs from other fat transplantation procedures in that it takes the patient's facial vascular paths into account while using their own fatty tissue to address the loss of volume in the face without having to make incisions. (wikipedia.org)
  • The adult stem cells are taken from an area of fatty tissue in a separate area of the body, typically from the patient's hip, knee or abdomen. (wikipedia.org)
  • However, the implanted biological pacemaker cells still typically need to be supplemented with an artificial pacemaker while the cells form the necessary electrical connections with cardiac tissue. (wikipedia.org)
  • Furthermore, there are vast developments in tissue engineering, which involve leveraging of technologies from biomaterials, molecular medicine, biochemistry, nanotechnology, genetic and biomedical engineering for regeneration and cell expansion targets to restructure and/or repair human organs. (wikipedia.org)
  • Adipose tissue host progenitor cells with reported interesting cardiomyogenic and vasculogenic potential in the sense that they improve heart functions and reduce infarction size in rodent animal models. (wikipedia.org)
  • Hence, research has been focused on induced pluripotent stem cells (iPSCs) from somatic human tissue. (wikipedia.org)
  • However, cardiac remodling remains limited due to limited cell residency, impact of mechanical forces onto cell survival and tissue hypoxia. (wikipedia.org)
  • Cardiac tissue engineering is a new technology based on the use of combinations of cells with regenerative capacity, biological and/or synthetic materials, cell signaling agents to induce the regeneration of an organ or damaged tissue. (wikipedia.org)
  • Adipose tissue (lipid cells), which requires extraction by liposuction. (wikipedia.org)
  • In practice, stem cells are identified by whether they can regenerate tissue. (wikipedia.org)
  • After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Cells can be isolated from solid tissues by digesting the extracellular matrix using enzymes such as collagenase, trypsin, or pronase, before agitating the tissue to release the cells into suspension. (wikipedia.org)
  • Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. (wikipedia.org)
  • endothelial cells
  • Our aim was to develop and characterize a highly reproducible rat syngeneic in vitro model of the BBB using co-cultures of primary rat brain endothelial cells (RBEC) and astrocytes to study receptors involved in transcytosis across the endothelial cell monolayer. (jove.com)
  • Recent studies[citation needed] have suggested that mammal hearts also host naturally occurring cardiac stem cells which are capable of differentiating themselves into cardiomyocytes, endothelial cells and cardiac fibroblasts. (wikipedia.org)
  • therapeutic
  • The current lack of tools for easy assessment of cancer stem cells (CSC) prevents the development of therapeutic strategies for hepatocellular carcinoma (HCC). (aacrjournals.org)
  • Non/less-invasive methods for assessing cancer stem cells (CSC) have yet to be developed, which has prevented the development of novel therapeutic strategies for hepatocellular carcinoma (HCC). (aacrjournals.org)
  • To help discover the therapeutic uses of these stem cells they are grown in laboratories or by using medication to stimulate new cell growth within the human body. (wikipedia.org)
  • Adoptive ex vivo transfer of EBNA-1-specific T cells is a feasible and well-tolerated therapeutic option, however for optimal efficacy expansion protocols should use antigenic sequences from relevant EBV strains. (wikipedia.org)
  • Mice
  • In this model, cells are directly implanted into the ventral lobe of the prostate in Balb/c athymic mice, and allowed to progress for 4-6 weeks. (jove.com)
  • Increased glomerular filtration rate and impaired contractile function of mesangial cells in TRPC6 knockout mice. (abcam.co.jp)
  • We have shown that γherpesvirus infection causes persistent virus infection, injury of lung epithelial cells, and pulmonary fibrosis in IFN-γR −/− mice with similar features to the human disease. (pubmedcentralcanada.ca)
  • It is important to mention that Long-Term Hematopoietic Stem Cells (LT-HSCs) in mice and humans are the hematopoietic cells with the greatest self-renewal capacity. (wikipedia.org)
  • Other genetic manipulation studies in mice indicate that the gene is required for the development and maintenance of bone marrow-based blood cell formation and the vascular network. (wikipedia.org)
  • This expression has the ability to produce B-cell lymphomas in transgenic mice and contribute to the survival of Burkitt's lymphoma in vitro. (wikipedia.org)
  • Transgenic mice expressing EBNA1 in B cell lines showed a predisposition for developing B cell lymphoma, thus demonstrating that EBNA1 is a viral oncogene and that it likely plays a role in B cell neoplasia. (wikipedia.org)
  • Experiments in alpha-sarcoglycan deficient dystrophic mice have shown that mesoangioblast transplantation can restore muscle function. (wikipedia.org)
  • Assays
  • Here we use tumor cell migration and invasion as an example and describe two related assays to illustrate the commonly used, easily accessible methods to measure these processes. (jove.com)
  • Stem cells can also be identified by functional assays. (wikipedia.org)
  • Below is a list of genes/protein products that can be used to identify various types of stem cells, or functional assays that do the same. (wikipedia.org)
  • Properties of stem cells can be illustrated in vitro, using methods such as clonogenic assays, in which single cells are assessed for their ability to differentiate and self-renew. (wikipedia.org)
  • fetal
  • Single-cell genome analyses reveal the amount of mutations a human brain cell will collect from its fetal beginnings until death. (the-scientist.com)
  • The first successful experiment with biological pacemakers was carried out by Arjang Ruhparwar's group at Hannover Medical School in Germany using transplanted fetal heart muscle cells. (wikipedia.org)
  • epithelial
  • Apoptosis of type II lung epithelial cells in aging lungs was accompanied by up-regulation of endoplasmic reticulum stress marker, binding immunoglobulin protein, and splicing of X-box-binding protein 1. (pubmedcentralcanada.ca)
  • These results indicate that the aging lung is more susceptible to injury and fibrosis associated with endoplasmic reticulum stress, apoptosis of type II lung epithelial cells, and activation of profibrotic pathways. (pubmedcentralcanada.ca)
  • We found that aging type II alveolar epithelial cells have sustained endoplasmic reticulum stress that causes severe apoptosis. (pubmedcentralcanada.ca)
  • EBNA1 has been linked to the epithelial to mesenchymal transition (EMT) in nasopharyngeal carcinoma cells. (wikipedia.org)
  • clinical
  • Role of amniotic fluid mesenchymal cells engineered on MgHA/collagen-based scaffold allotransplanted on an experimental animal study of sinus augmentation," Clinical Oral Investigations , vol. 17, no. 7, pp. 1661-1675, 2013. (hindawi.com)
  • 6 Clinical trials with stem cells. (docplayer.net)
  • Strategic decision to target an easier disease to get cells into clinical trial = Type-1 diabetes. (docplayer.net)
  • Newswise - Baltimore, MD - December 20, 2016 - In a first-in-children randomized clinical study, medical researchers at the University of Maryland School of Medicine (UM SOM) and the Interdisciplinary Stem Cell Institute (ISCI) at the University of Miami Miller School of Medicine have begun testing to see whether adult stem cells derived from bone marrow benefit children with the congenital heart defect hypoplastic left heart syndrome (HLHS). (newswise.com)
  • Among progressing the use of stem cells for translational purposes, aiming at tapping the potential of stem cell biology towards clinical application, Hescheler actively promotes the vision of EPAA through innovative alternative testing strategies, applying stem cells towards toxicity testing. (wikipedia.org)
  • Mechanisms
  • Therefore, an ideal treatment must possess both immunomodulating properties to control irregular autoimmune responses to prevent further damage and regenerative properties to stimulate natural repair mechanisms and replace damaged cells. (wikipedia.org)
  • Two mechanisms exist to ensure that a stem cell population is maintained: Obligatory asymmetric replication: a stem cell divides into one mother cell that is identical to the original stem cell, and another daughter cell that is differentiated. (wikipedia.org)
  • Biology
  • Methods to examine cell migration are very useful and important for a wide range of biomedical research such as cancer biology, immunology, vascular biology, cell biology and developmental biology. (jove.com)
  • It is our goal to describe these methods in a highly accessible manner so that the procedures can be successfully performed in research laboratories even just with basic cell biology setup. (jove.com)
  • Electron cryo-tomography is a powerful tool in structural biology, capable of visualizing the three-dimensional structure of biological samples, such as cells, organelles, membrane vesicles, or viruses at molecular detail. (jove.com)
  • vitro
  • 10,000 cells are harvested and grown in vitro for approximately six weeks until the population reaches 10-12 million cells. (wikipedia.org)
  • These cells are then grown under controlled in vitro conditions in a lab, so that they can multiply and same time mature( also referred to as differentiated. (wikipedia.org)
  • grown
  • The Interdisciplinary Stem Cell Institute has grown from a local research center to a national cell manufacturing facility. (newswise.com)
  • Stem cells can now be artificially grown and transformed (differentiated) into specialized cell types with characteristics consistent with cells of various tissues such as muscles or nerves. (wikipedia.org)
  • Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. (wikipedia.org)
  • Most cells require a surface or an artificial substrate (adherent or monolayer culture) whereas others can be grown free floating in culture medium (suspension culture). (wikipedia.org)
  • Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37 °C, 5% CO2 for mammalian cells) in a cell incubator. (wikipedia.org)
  • cardiomyocytes
  • By specific inhibition of Ik1 below a certain level, spontaneous activity of cardiomyocytes was observed with resemblance to the action potential pattern of genuine pacemaker cells. (wikipedia.org)
  • genes
  • In the present study, MMP-9, uPAR and cathepsin B genes were downregulated in glioma xenograft cells using shRNA plasmid constructs and we evaluated the involvement of integrins and changes in their adhesion, migration and invasive potential. (jove.com)
  • Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. (wikipedia.org)
  • Both mutant proteins failed to enter cell nuclei normally and had a reduced capacity to target genes regulated by the normal ETV6 protein. (wikipedia.org)
  • EBNA1 is the only identified latent protein-encoding genes that it consistently expressed in Burkitt's lymphoma cells and is believed to contribute to EBV malignancies through B cell-directed expression. (wikipedia.org)
  • tumors
  • With the exception of some derived from tumors, most primary cell cultures have limited lifespan. (wikipedia.org)
  • regeneration
  • In order to reduce the length of time for regeneration, further research is being conducted to speed regeneration such as nerve grafts and stem cells. (wikipedia.org)
  • monolayer
  • The first method is the cell culture wound closure assay in which a scratch is generated on a confluent cell monolayer. (jove.com)
  • From a cellular perspective, available techniques are monolayer cell construct onto temperature-sensitive polymer, where their detachment is driven by behavior of the mechanical properties of the synthetic support without the need of any enzymatic digestion such as trypsin. (wikipedia.org)
  • cardiac
  • Nevertheless, all methods aforementioned have been associated with limited cardiac function improvements and limited cell survival once implanted in the fibrous myocardium. (wikipedia.org)
  • CD34
  • CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. (wikipedia.org)
  • Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
  • Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. (wikipedia.org)
  • Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration. (wikipedia.org)
  • citation needed] CD34 is expressed in roughly 20% of murine hematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
  • vivo
  • Recent advances in cell surface and intracellular marker identification and single-cell analyses led to greater resolution and high-throughput ex-vivo quantification. (wikipedia.org)
  • isolate
  • It should also be possible to isolate stem cells from the transplanted individual, which can themselves be transplanted into another individual without HSCs, demonstrating that the stem cell was able to self-renew. (wikipedia.org)
  • characterization
  • At experiment termination, several distinct endpoints can be measured, such as size and molecular characterization of the primary tumor, the presence and quantification of circulating tumor cells in the blood and bone marrow, and formation of metastasis to the lung. (jove.com)
  • iPSCs
  • Resulting iPSCs were further characterized and deemed free of transfected DNA, integrated transgene DNA, and lack detectable gene rearrangements such as those within the immunoglobulin heavy chain and T cell receptor loci of more differentiated cell types. (jove.com)
  • Research
  • This is the first HLHS research in the United States to use stem cells known as allogeneic mesenchymal stem cells (MSC). (newswise.com)
  • Research has shown that CD34+ cells are relatively more in men than in women in the reproductive age among Spinal Cord Injury victims. (wikipedia.org)
  • citation needed] Furthermore, he is founder and chairman to the German Society for Stem Cell Research (GSZ). (wikipedia.org)
  • He thus pioneered the establishment of stem cell research for application in transplantation medicine. (wikipedia.org)
  • In 2004 he founded and since chaired the German Society of Stem Cell Research (GSZ). (wikipedia.org)
  • Research into stem cells grew out of findings by Ernest A. McCulloch and James E. Till at the University of Toronto in the 1960s. (wikipedia.org)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • gene
  • Promoter CpG methylation contributes to ES cell gene regulation in parallel with Oct4/Nanog, PcG complex, and histone H3 K4/K27 trimethylation," Cell Stem Cell , vol. 2, no. 2, pp. 160-169, 2008. (hindawi.com)
  • This potential ability is suppressed by the inward-rectifier potassium current Ik1 encoded by the gene Kir2 which is not expressed in pacemaker cells. (wikipedia.org)
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)