• cytoplasmic
  • Because the sequence identity of malate dehydrogenase in the mitochondria is more closely related to its prokaryotic ancestors in comparison to the cytoplasmic isozyme, the theory that mitochondria and chloroplasts were developed through endosymbiosis is plausible. (wikipedia.org)
  • nadp
  • C4 NADP-ME has been shown to be partially inhibited by its substrate, malate, suggesting two independent binding sites: one at the active site and one at an allosteric site. (wikipedia.org)
  • K-m for malate was 100 mu M and 458 mu M in the presence of NAD(+) and NADP(+), respectively. (uclouvain.be)
  • K-m for NAD(+) and for NADP(+) in the presence of malate was 18 mu M and 207 mu M, respectively. (uclouvain.be)
  • MDH2
  • When both malate and NAD concentrations are high (10 mmol/l and 5 mmol/l, respectively), citrate can actually augment MDH2 activity. (wikipedia.org)
  • Since MDH2 plays a major role in malate-aspartate shuttling in ATP production, its overexpression likely supplies additional energy for P-glycoprotein to pump chemotherapeutic drugs out of the cells. (wikipedia.org)
  • substrates
  • Additionally, a key arginine residue at site 237 has been shown to interact both with malate and NADP+ substrates, forming key favorable electrostatic interactions to the negatively charged carboxylic-acid and phosphate group respectively. (wikipedia.org)
  • shuttle
  • In a study completed in 2008, inhibiting the malate-aspartate shuttle was found to impair the glycolysis process and essentially decreased breast adenocarcinoma cell proliferation. (wikipedia.org)
  • Thus
  • Thus, glutamate can react with the aminotransferase in this complex without glutamate inhibiting production of oxalacetate by the malate dehydrogenase in the complex. (wikipedia.org)