Luciferases firefly. Medical search. Frequent questions

Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. (wikipedia.org)
Luciferase can function in two different pathways: a bioluminescence pathway and a CoA-ligase pathway. (wikipedia.org)
Firefly luciferase bioluminescence color can vary between yellow-green (λmax = 550 nm) to red (λmax = 620). (wikipedia.org)
D-luciferin is the substrate for firefly luciferase's bioluminescence reaction, while L-luciferin is the substrate for luciferyl-CoA synthetase activity. (wikipedia.org)
The customized firefly luciferase portrayed Celastrol kinase inhibitor in (Mut2) was discovered to significantly raise the awareness of bioluminescence imaging (BLI) in systemic attacks when compared with unmodified luciferase (Mut0). (thetechnoant.info)
Luciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. (thomassci.com)
Available in a variety of promoter-reporter combinations that enable multiple imaging modalities-fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)-you can select from lentivector or minicircle technologies for both integrated and episomal expression. (reportergene.com)
Firefly luciferase bioluminescence (FLB) is a highly sensitive and specific method for the analysis of adenosine-5-triphosphate (ATP) in biological samples. (ias.ac.in)
Upon receptor activation and subsequent β-arrestin2 recruitment, an active luciferase enzyme complex is produced, which can be detected by in vivo bioluminescence imaging. (nih.gov)
When these reactions occur in living organisms such as bacteria, fireflies, squid and deep-sea fishes, the process is known as bioluminescence . (howstuffworks.com)

The enzyme catalyses the oxidation of firefly luciferin, requiring oxygen and ATP. (wikipedia.org)
The chemical reaction catalyzed by firefly luciferase takes place in two steps: luciferin + ATP → luciferyl adenylate + PPi luciferyl adenylate + O2 → oxyluciferin + AMP + light Light is produced because the reaction forms oxyluciferin in an electronically excited state. (wikipedia.org)
Firefly luciferase generates light from luciferin in a multistep process. (wikipedia.org)
This shows that luciferase can differentiate between the isomers of the luciferin structure. (wikipedia.org)
The synthesized luciferase catalyzes the luminescent reaction of luciferin with O2 and ATP. (uncg.edu)
The enzyme catalyzes the oxidation of reduced luciferin in the presence of ATP-Mg 2+ and oxygen to generate CO 2 , AMP, PPi, oxyluciferin and produces a flash of light that is proportional to the quantity of luciferase in the reaction mixture. (canvaxbiotech.com)
The Luciferase Assay Substrate includes coenzyme A, ATP and luciferin. (canvaxbiotech.com)
Included in these are the luciferase uses coelenterazine as air and substrate as co-factors to emit light, as the click firefly and beetle luciferases use luciferin and ATP as co-factors. (thetechnoant.info)
The methods described here are based on the use of measuring ATP content by luciferin-luciferase assay to indicate an increased number of proliferating cells in the draining auricular lymph nodes. (nih.gov)
The luciferin-luciferase assay is a sensitive method for ATP quantitation used in a wide variety of applications (Lundin 2000). (nih.gov)
First, the luciferase catalyzes the oxidation of luciferin. (howstuffworks.com)
Luciferases from FIREFLIES , usually Photinus, that oxidizes FIREFLY LUCIFERIN to cause emission of PHOTONS . (nih.gov)

Firefly luciferase (FLuc), an ATP-dependent bioluminescent reporter enzyme, is broadly used in chemical biology and drug discovery assays. (nih.gov)
2009). On the other hand, firefly luciferase can only just end up being an intracellular enzyme, because it requirements ATP made by inner metabolic activity for light emission. (thetechnoant.info)
Firefly luciferase refers to the enzyme that was isolated from Photinus pyralis. (thomassci.com)
Researchers emphasized the clinical implications of their findings are unclear, but said the results suggest more work may be needed to make sure the screening process to select promising agents was not flawed by its effects on a firefly enzyme used as a marker. (nih.gov)
The enzyme that makes fireflies glow, called luciferase, is widely used in biomedical experiments and in high-throughput screening often utilized to discover drugs. (nih.gov)
In the PTC screen, a particular group of test compounds elicited very bright signals from the firefly enzyme. (nih.gov)
However, the NCGC researchers determined that some luciferase inhibitors-including PTC124 and related compounds belonging to the 3,5-diaryl-oxadiazole family-actually increase the signal's brightness by binding to the firefly enzyme in a way that slows its degradation within cells. (nih.gov)
They did this by borrowing the light from fireflies, adding genes from the insects that express an enzyme called luciferase. (popsci.com)
They use the term luciferase to describe the enzyme that drives, or catalyzes, the reaction. (howstuffworks.com)

Luciferase Assay Kit (from the firefly Photinus pyralis ) is an accurate, sensitive and easy method for studying gene reporter regulation and function in transformed cell lines in culture. (canvaxbiotech.com)

We also demonstrate that the inhibitory activity of PTC124-AMP is relieved by free coenzyme A, a component present at high concentrations in luciferase detection reagents used for cell-based assays. (nih.gov)
Hybridization assays using as a label an expressible DNA fragment encoding firefly luciferase. (uncg.edu)
Luciferase assays are quick, highly sensitive, have wide dynamic range, and are cheap to perform. (cdc.gov)

To address this issue, we transduced CD34-selected UCB cells with a lentiviral construct expressing luciferase, and determined homing and engraftment patterns in vivo by noninvasive bioluminescent imaging in sublethally irradiated NOD/SCID/IL-2Rgamma(-/-) (NSG) mice. (nih.gov)
A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. (springer.com)
Cypridina and Gaussia are both secreted luciferases, producing high bioluminescent signal intensity. (neb.com)
S1P1 luciferase signaling mice enable the bioluminescent detection of sphingosine-1-phosphate receptor 1 (S1P1) activation in real time. (nih.gov)
The researchers packaged mRNA instructions for firefly luciferase-a bioluminescent protein often used in research-into lipid nanoparticles, and then injected them into mice either intravenously or intraperitoneally. (nih.gov)
To address this issue, we used in vivo bioluminescent imaging to track the fate of transplanted hESCs stably transduced with a double-fusion reporter gene consisting of firefly luciferase and enhanced GFP. (ca.gov)

Pre-made lentiviral particles expressing firefly luciferase under EF1a promoter with Blastidicin selection marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. (reportergene.com)
Our primary goal for the present study was to evaluate the effect of lentiviral infection of GL261 cells, for expression of firefly luciferase, on immunomodulatory cytokine expression and for tumor growth in vivo . (biomedcentral.com)
Lentiviral particles with Renilla Luciferase as the reporter gene for binding of CBF1 / RBP-JK to RBP-JK TRE. (nucleusbiotech.com)

Using a split luciferase reporter system to measure protease activity, we determined that polyamines facilitate viral protease activity. (mdpi.com)
A synthetic S1P1 signaling pathway, designed to report the interaction between S1P1and β-arrestin2 via the firefly split luciferase fragment complementation system, is genetically encoded in these mice. (nih.gov)

The DNA label contains a firefly luciferase coding sequence downstream from a T7 RNA polymerase promoter. (uncg.edu)

Following addition of 50?l/well Nano-Glo Luciferase Assay Substrate (Promega) light emission was recorded utilizing a BMG Labtech Fluostar dish audience and data analysed such as Section 2.5. (bioinf.org)

This lentivirus expresses Firefly luciferase under the control of CMV promoter. (thomassci.com)
The CMV-Luciferase-EF1α-copGFP vector expresses luciferase from a CMV promoter for high expression in most cell types, and the bright copGFP from the EF1α promoter for medium expression. (reportergene.com)
This is achieved by linking the firefly luciferase gene to a promoter sequence. (cdc.gov)
Transformed from Human ES2 cell line , the firefly Luciferase 3 and RFP fluorescent report was constitutively co-expressed under the enhanced EF1a promoter as two separate proteins (not as fusion), mediated via F2A element. (gentarget.com)
Promoterless vector encoding highly destabilized luciferase for measuring the activity of promoter and enhancer sequences. (snapgene.com)

The protein structure of firefly luciferase consists of two compact domains: the N-terminal domain and the C-terminal domain. (wikipedia.org)
We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase. (springer.com)
Therefore, for further experiments, we decided to use the yeast strain, producing luciferase with a sequence of six histidine residues at the C-terminus of the protein. (springer.com)
Moreover, PTC124 was shown to increase the amount and function of critical proteins in animal and cell-culture models that do not rely on firefly luciferase as a marker, namely, the cystic fibrosis transmembrane conductance regulator protein in models of cystic fibrosis and the dystrophin protein in models of Duchenne muscular dystrophy. (nih.gov)
For that they used two different proteins: firefly luciferase and a truncated version of an avian reovirus protein, called muNS, fused to GFP, which allowed them to monitor by fluorescence the initial steps of the crystal formation in the cells. (nanowerk.com)
To observe the RNA inhibition process, McCaffrey injected mice with a firefly gene called luciferase that makes a light-producing protein. (stanford.edu)
In the experiments scientists used DNA of a gene, coding luciferase, a glowing protein, belonging to fireflies. (russia-ic.com)

During a reaction, luciferase has a conformational change and goes into a "closed" form with the two domains coming together to enclose the substrate. (wikipedia.org)

Further, considering that the luciferase is certainly a secreted proteins normally, a modified edition has been portrayed such that it will localize on the cell wall structure through the addition of a GPI-anchor (Enjalbert et al. (thetechnoant.info)
PTC Therapeutics of South Plainfield, N.J., identified PTC124 through a cell-based screening system that used firefly luciferase to gauge the power of chemical compounds to enable cells with nonsense mutations to produce normal, full-length proteins. (nih.gov)

The CMV-Luciferase-EF1α-copGFP BLIV 2.0 Lentivector for In Vivo Imaging leverages SBI's well-regarded lentivector technology for either transduction or transfection of the reporter into target cells. (reportergene.com)
6. In vivo imaging of ligand receptor binding with Gaussia luciferase complementation. (nih.gov)
Morphology and intracellular localization of in vivo firefly luciferase crystals. (nanowerk.com)
Luciferase expression in GL261 murine glioma cells does not affect GL261 proliferation, invasion, cytokine expression, or in vivo growth. (biomedcentral.com)

12. Imaging CXCR4 signaling with firefly luciferase complementation. (nih.gov)

GL261 cells were infected with lentivirus containing a gene encoding firefly luciferase (GL261.luc). (biomedcentral.com)

One of those compounds was then optimized, also using firefly luciferase tests, to develop the experimental drug PTC124. (nih.gov)
Now, in their study published in PNAS, researchers from the NIH Chemical Genomics Center (NCGC) present results that may have significant implications for the process used to identify PTC124, as well as other drug discovery efforts dependent upon firefly luciferase tests. (nih.gov)
The NCGC's findings related to PTC124 arose from the center's efforts to optimize its own screening process by characterizing the types of chemical compounds that interfere with firefly luciferase. (nih.gov)

Because of their similar activities, luciferase is able to replace fatty acyl-CoA synthetase and convert long-chain fatty acids into fatty-acyl CoA for beta oxidation. (wikipedia.org)

The inoculums were washed out, and Gaussia luciferase activity and cellular ATP levels were measured at 30 h postinfection. (medscape.com)
Cypridina can be used in combination with Gaussia, Renilla and Firefly luciferases and lacZ reporter in a co-transfection. (neb.com)
For more information on Gaussia Luciferase products, please click here . (neb.com)

In half the mice, he also injected RNA that inhibits luciferase production. (stanford.edu)

Similarly, other results have indicated that the microenvironment of luciferase can force oxyluciferin into a more rigid, high-energy structure, forcing it to emit a high-energy green light. (wikipedia.org)

The ABP Firefly Luciferase Assay Kit provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. (mobitec.com)
correlation between luciferase gene expression and light output for transfection. (canvaxbiotech.com)
Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast and inexpensive tool for the determination of dioxin-like compounds in a large number of samples and requires only small sample volumes, the use of this technique in human biomonitoring programs provides a good alternative to GC-HRMS. (nih.gov)
Luciferase reporter system for studying the effect of nanoparticle s on gene expression. (cdc.gov)
Genetic Luciferase Reporter System or Reporter gene assay has become an invaluable tool in studies of gene expression. (cdc.gov)
Here we describe the Genetic Luciferase Reporter System as the method for detecting alteration of gene expression in response to external stimuli (e.g., nanoparticle s). (cdc.gov)

Examples of how SBI's BLIV lentivectors deliver strong luciferase activity after both transduction and transfection. (reportergene.com)
Finally, quantifying virus-encoded firefly luciferase activity in cell lysates was utilized to determine the efficacy of transduction. (news-medical.net)

Because of their simplicity and versatility, and because of the absence of endogenous luciferase activity in most cell types, this test can be applied for testing a large variety of nanomaterial s for their pathogenic or carcinogenetic effects on a wide range of mammalian cells. (cdc.gov)

Examples of how SBI's BLIV minicircle vectors deliver strong luciferase activity in small animal models. (reportergene.com)
Examples of how SBI's BLIV lentivectors and minicircle vectors can be used to generate stable cell lines with strong luciferase activity. (reportergene.com)

In both pathways, luciferase initially catalyzes an adenylation reaction with MgATP. (wikipedia.org)

There are currently several different mechanisms describing how the structure of luciferase affects the emission spectrum of the photon and effectively the color of light emitted. (wikipedia.org)
We modified the firefly luciferase to be able to improve its optimum emission strength in debt light range (600C700 nm) aswell concerning improve its thermostability in mice. (thetechnoant.info)
As the emission top of luciferase reaches 480 nm, firefly luciferase comes with an emission top at around. (thetechnoant.info)

In the ongoing function shown right here, we utilized a codon-optimized Celastrol kinase inhibitor firefly luciferase reporter program for discovering in mice. (thetechnoant.info)
To conclude, BLI using a red-shifted firefly luciferase was discovered to be always a effective tool for tests the destiny of in a variety of mice infection versions. (thetechnoant.info)
In mice receiving both luciferase and the RNA, whole-body scans showed 80 percent to 90 percent less light compared to mice that received the luciferase gene alone. (stanford.edu)
In a related experiment, McCaffrey hooked the luciferase gene to a small part of a gene from the hepatitis C virus and injected the hybrid gene into mice along with RNA that is specific to the DNA found in hepatitis C. Once again, mice that received both the gene and the RNA produced significantly less light than mice receiving only the luciferase gene. (stanford.edu)
Injecting this novel RNA into mice was as effective at inhibiting the luciferase gene as injecting double-stranded RNA. (stanford.edu)
A complex molecule, consisting of DNA of a luciferase gene and a hybrid peptide, was introduced into caudal (tail) vein of pregnant female mice by means of hydrodynamic DNA injection. (russia-ic.com)

The amino acid sequences on the surface of the two domains facing each other are conserved in bacterial and firefly luciferase, thereby strongly suggesting that the active site is located in the cleft between the domains. (wikipedia.org)

Detection and quantification of Firefly Luciferase. (canvaxbiotech.com)

The genes that control the luciferase activated during different parts of the circadian cycle, providing a bright cue for a process that is otherwise invisible. (popsci.com)
Of the 82 genes examined by RT-PCR array, seven (9%) exhibited statistically significant change after luciferase modification. (biomedcentral.com)

Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes. (nih.gov)

Firefly Luciferase has an apparent molecular weight of 62 kDa, which is active as a monomer and does not require subsequent processing for its activity. (canvaxbiotech.com)
The Firefly luciferase activity and cellular ATP levels were measured. (medscape.com)
Relative luciferase activity was normalized with cellular ATP levels. (medscape.com)

Modification of tumor cells for luciferase expression enables non-invasive monitoring of orthotopic tumor growth, and has proven useful for studying glioblastoma response to novel therapeutics. (biomedcentral.com)
However, tumor modification for luciferase has the potential for evoking host immune response against otherwise syngeneic tumor cells, thereby mitigating the tumor cells' value for tumor immunology and immunotherapy studies. (biomedcentral.com)

Because of the requirement of ATP, firefly luciferases have been used extensively in biotechnology. (wikipedia.org)

The lysate was centrifuged (8000 g , 30 min) at 4°C and the membrane fraction containing luciferase was pelleted by ultracentrifugation (150 000 g , 90 min) at 4°C. To select the conditions for luciferase solubilization, the membranes were suspended in 50 mM HEPES-Na buffer (pH 8.0) containing 500 mM NaCl, 20% glycerol (buffer A), and various detergents at 4°C overnight ( Fig. 1 ). (springer.com)
Adsorbed luciferase was eluted with buffer B containing 0.1% DDM and 200 mM imidazole. (springer.com)

Organisms9

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes11

Disciplines and Occupations1

Information Science2

Bioluminescence10

Luciferin12

Enzyme9

Photinus1

Assays3

Bioluminescent6

Lentiviral3

Split luciferase2

Coding sequence1

Assay Substrate1

Promoter5

Protein7

Substrate1

Proteins2

Vivo4

Complementation1

Lentivirus1

PTC1243

Oxidation1

Gaussia3

Inhibits1

Oxyluciferin1

Gene expression6

Transduction2

Mammalian cells1

Vectors2

Catalyzes1

Emission3

Mice6

Bacterial1

Detection1

Genes2

Light1

Activity3

Cells2

Biotechnology1

Buffer2