• interphase
  • The subcellular localization of chmadrin was found to be similar to that of pKi-67 throughout the cell cycle, that is, predominantly nucleolar during interphase and perichromosomal in the mitotic phase. (biologists.org)
  • chromosomes
  • Residues 494-778, which show extensive similarity to the corresponding region of pKi-67, were efficiently targeted to nucleoli, whereas a repetitive structure found at the C-terminal portion, whose similarity to pKi-67 is weak, was localized precisely to mitotic chromosomes. (biologists.org)
  • retrieval
  • Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. (abcam.com)
  • One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval (see next section below). (wikipedia.org)
  • marker
  • In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy Ki-67 is an excellent marker to determine the growth fraction of a given cell population. (wikipedia.org)
  • Human
  • Synthetic peptide within Human Ki67 aa 2300-2400 (C terminal). (abcam.com)
  • There are two conserved sequence motifs within the FHA Ki67 binding domain: TPVCTP and LERRKS, this domain binds to the forkhead-associated domain of human Ki67. (wikipedia.org)
  • index
  • Large size, extraadrenal location, dopamine secretion, SDHB mutations, a PASS score higher than 6, a high Ki-67 index are indexes for potential malignancy. (hindawi.com)
  • There are also many histological differences with variability in type, grade, mitotic index and Ki67. (biomedcentral.com)
  • MIB-1 is used in clinical applications to determine the Ki-67 labelling index. (wikipedia.org)
  • Prostate
  • They are negative for androgen receptor and prostate-specific antigen (PSA) and are positive for prostate acid phosphatase. (wikipedia.org)
  • Immunohistochemically, prostatic small cell carcinoma are positive for thyroid transcription factor 1 (TTF-1), CD56, chromogranin A, synaptophysin, neuron-specific enolase, calcitonin and bombesin/gastrin-releasing peptide, while lacking, or rarely and weakly expressing, androgen receptor and prostate-specific antigen. (wikipedia.org)
  • domain
  • The C-terminal portion was designated the 'LR domain' since several LR (leucine and arginine) pairs commonly appear in chmadrin and pKi-67. (biologists.org)
  • MKI67 FHA domain-interacting nucleolar phosphoprotein contains an RNA recognition motif (RRM) near to the N-terminus and a FHA Ki67 binding domain near to the C-terminus. (wikipedia.org)
  • found
  • The MART-1/melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. (wikipedia.org)
  • Mouse
  • Immunofluorescent antibody staining against neurofilament (green) and Ki-67 (red) in a mouse embryo 12.5 days after fertilization. (wikipedia.org)