Although ketosteroid isomerase (KSI) is a commonly used affinity tag for expression and purification of short peptides, KSI tag needs to be removed with the toxic reagent cyanogen bromide (CNBr). (a-star.edu.sg)
Quantitative dissection of hydrogen bond-mediated proton transfer in the ketosteroid isomerase active site. (cornell.edu)
We have carried out in-depth interrogations of the proton transfer equilibrium within a hydrogen bond network formed to bound phenols in the active site of ketosteroid isomerase. (cornell.edu)
134. P. Hanoian, P. A. Sigala, D. Herschlag, and S. Hammes-Schiffer, "Hydrogen bonding in the active site of ketosteroid isomerase: Electronic inductive effects and hydrogen bond coupling," Biochemistry 49 , 10339-10348 (2010). (hammes-schiffer-group.org)
The enzyme 3 beta-hydroxysteroid dehydrogenase/5-ene-4-ene isomerase (3 beta-HSD) catalyses the oxidation and isomerisation of 5-ene-3 beta-hydroxypregnene and 5-ene-hydroxyandrostene steroid precursors into the corresponding 4-ene-ketosteroids necessary for the formation of all classes of steroid hormones. (embl.de)
However, the structure of the ketosteroid isomerase had to be refined in P1 at atomic resolution, although it refines well in C2221 at lower resolution such as 1.5A. (uni-konstanz.de)
Steroids1
5beta-Reduced steroids and human Delta(4)-3-ketosteroid 5beta-reductase (AKR1D1). (medlineplus.gov)
Examples of 17-ketosteroids include: Androstenedione Androstanedione Androsterone Dehydroepiandrosterone Epiandrosterone Epietiocholanolone Etiocholanolone 17-Ketosteroids are endogenous steroid hormones. (wikipedia.org)
Ketone2
A ketosteroid, or an oxosteroid, is a steroid in which a hydrogen atom has been replaced with a ketone (C=O) group. (wikipedia.org)
A 17-ketosteroid is a ketosteroid in which the ketone is located specifically at the C17 position (in the upper right corner of most structure diagrams). (wikipedia.org)
1953 ) Acylation of 17-hydroxy-20-ketosteroids Journal of the American Chemical Society . (academictree.org)
Tests1
Newer tests have superseded 17-ketosteroid testing, and the assay is seldom used now. (medscape.com)
Total1
This graph shows the total number of publications written about "17-Ketosteroids" by people in this website by year, and whether "17-Ketosteroids" was a major or minor topic of these publications. (rush.edu)
Reductase5
AKR1D1 (steroid 5beta-reductase) reduces all Delta(4)-3-ketosteroids to form 5beta-dihydrosteroids, a first step in the clearance of steroid hormones and an essential step in the synthesis of all bile acids. (nih.gov)
The reduction of the carbon-carbon double bond in an alpha,beta-unsaturated ketone by 5beta-reductase is a unique reaction in steroid enzymology because hydride transfer from NADPH to the beta-face of a Delta(4)-3-ketosteroid yields a cis-A/B-ring configuration with an approximately 90 degrees bend in steroid structure. (nih.gov)
Here, we report the first x-ray crystal structure of a mammalian steroid hormone carbon-carbon double bond reductase, human Delta(4)-3-ketosteroid 5beta-reductase (AKR1D1), and its complexes with intact substrates. (nih.gov)
The activity of 3 beta-hydroxysteroid dehydrogenase/isomerase (3 beta-HSD), 17-hydroxylase (17-OHase), 17,20-desmolase (17,20D), 17-ketosteroid reductase (17-KSR) and aromatase were measured using a constant amount (50 microM) of 14C-labelled substrates in the presence of varying concentrations of pure ketoconazole. (regrowth.com)
The glutamate-histidine substitution prevents a 3-ketosteroid from penetrating the active site so that hydride transfer is directed toward the C3 carbonyl group rather than the Δ(4)-double bond and confers 3β-HSD activity on the 5β-reductase. (anl.gov)
Progesterone1
The substrate spectrum of N. simplex 3-ketosteroid-Δ 1 -dehydrogenase was expanded in this study with progesterone and its 17α-hydroxylated and 11α,17α-dihydroxylated derivatives, that effectively were 1(2)-dehydrogenated in vivo by the whole cells of the N. simplex VKM Ac-2033D. (biomedcentral.com)
Steroid4
A ketosteroid, or an oxosteroid, is a steroid in which a hydrogen atom has been replaced with a ketone (C=O) group. (wikipedia.org)
Examples of 17-ketosteroids include: Androstenedione Androstanedione Androsterone Dehydroepiandrosterone Epiandrosterone Epietiocholanolone Etiocholanolone 17-Ketosteroids are endogenous steroid hormones. (wikipedia.org)
17-ketosteroids are substances that form when the body breaks down male steroid sex hormones called androgens and other hormones released by the adrenal glands in males and females, and by the testes in males. (medlineplus.gov)
A study by Adriaansen et al suggested that salivary samples of the 17-ketosteroid androstenedione as well as of another steroid, 17-hydroxyprogesterone, can be used to monitor the efficacy of treatment in congenital adrenal hyperplasia. (medscape.com)
Receptors1
6. Human ketosteroid receptors interact with hazardous phthalate plasticizers and their metabolites: an in silico study. (nih.gov)
Hydroxysteroid1
AKR1D1 catalyzes the 5β-reduction of Δ(4)-3-ketosteroids, whereas AKR1C enzymes are hydroxysteroid dehydrogenases (HSDs). (anl.gov)
Hammond GL, 1995) whereas C19 17-ketosteroids such as dehydroepiandrosterone (DHEA) and androstendione do not bind so easily. (cdc.gov)
An important point is not vigorous at birth, vitamin d - hydroxycholecalciferol - oh pregnenolone, desmolase dhea - ol dehydrogenase urinary ketosteroids dhea elevated plasma bicarbonate concentration or carrying capacity is maintained. (elastizell.com)
Enzymes1
The genome sequence of R.pyridinivorans strain AK37 has identified several key enzymes involved in the six pathways of monocyclic aromatic compound biodegradation: protocatechuate 3,4-dioxygenase, benzoate 1,2-dioxygenase, 3-ketosteroid-9α-hydroxylase, 3-ketosteroid-σ-dehydrogenase, and so on. (kenyon.edu)
The strain of Nocardioides simplex VKM Ac-2033D is well known mainly for its superior 3-ketosteroid Δ 1 -dehydrogenase activity towards various 3-oxosteroids and other important reactions of sterol degradation. (biomedcentral.com)
Superior 3-ketosteroid-Δ 1 -dehydrogenase activity of N. simplex VKM Ac-2033D is due to the kstDs redundancy in the genome, with the highest expression level of the gene KR76_27125 orthologous to kstD2, in response to cortisone 21-acetate. (biomedcentral.com)
Search1
Search Medline for 3-ketosteroid 9alpha-hydroxylase . (ethz.ch)