This cassette consists of the SV40 early promoter (PSV40 e), a Tn5 kanamycin/neomycin resistance gene, and herpes simplex virus thymidine kinase (HSV TK) polyadenylation signals. (biofeng.com)
A neomycin resistance cassette (Neor ) consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV-TK) gene allow stably transfected eukaryotic cells to be selected using G418 (7). (youbio.cn)
Thymidine1
Mammalian vector with a constitutive HSV thymidine kinase promoter for expressing secreted Gaussia luciferase. (snapgene.com)
Tyrosine6
The central core region of APH(3') has a high degree of conformational similarity to regions of serine/tyrosine and threonineprotein kinases, functionally equivalent enzymes found in eukaryotes. (wikipedia.org)
Several studies have suggested that serine/tyrosine/threonine protein kinases, once thought to only occur in eukaryotes, are also found in the prokaryotes. (wikipedia.org)
Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is a type 1 proteins expressed on chronic lymphocytic leukemia (CLL) B cells, however, not on regular postpartum tissue. (rawveronica.com)
BTK, a TEC-family tyrosine kinase activated by the B-cell antigen receptor, contains a variety of regulatory domains and it is subject to complex regulation by membrane phospholipids, protein ligands, phosphorylation, and dimerization. (elifesciences.org)
The TEC family kinase, BTK (Bruton's tyrosine kinase) is best known as the target of ibrutinib (IMBRUVICA®), the first-in-class covalent kinase active site inhibitor used to treat chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), Waldenström's macroglobulinemia (WM), and chronic graft-versus-host disease (cGVHD). (elifesciences.org)
The TEC kinases are the second largest sub-family of non-receptor tyrosine kinases in the human genome after the SRC family [ 1 - 3 ]. (elifesciences.org)
Phosphotransferase4
Aminoglycoside-3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an enzyme that primarily catalyzes the addition of phosphate from ATP to the 3'-hydroxyl group of a 4,6-disubstituted aminoglycoside, such as kanamycin. (wikipedia.org)
The systematic name of this enzyme class is ATP:kanamycin 3'-O-phosphotransferase. (wikipedia.org)
This enzyme is also called neomycin-kanamycin phosphotransferase. (wikipedia.org)
A selectable marker, neomycin phosphotransferase II, conferring resistance to kanamycin, was transferred into sugar pine using a binary armed vector system. (tamu.edu)
Nucleoside diphos1
Nucleoside diphosphate kinase (NDK), which is widely conserved in both prokaryotes and eukaryotes, maintains a balanced pool of nucleotide triphosphates and their deoxy derivatives. (openbiochemistryjournal.com)
Aspartate kinase1
widespread and biotechnologically important aspartate kinase enzymes. (apoptosisblog.com)
Gene5
Assignment of the gene for adenosine kinase to chromosome 14 in Mus musculus by somatic cell hybridization. (ucdenver.edu)
Design: Allelic replacement via homologous recombination of the STPK encoding gene with a kanamycin resistant determinant has been constructed. (elsevierpure.com)
The allelic substitute mutant, VT1346, was generated by insertion of the kanamycin level of resistance (Kmr) gene, as previously defined (17). (careersfromscience.org)
In this statement, we describe cloning and Asunaprevir kinase activity assay nucleotide sequencing of the 5 endoglucanase Cel5A gene (5 used in this study was kindly supplied by N.O. van Glyswyk (National Chemical Study Laboratory, Pretoria, South Africa). (insulin-receptor.info)
Protein kinases2
Additionally, X-ray crystallography and mutagenesis of key active site residues supports claims that APH(3') and eukaryotic protein kinases are related, despite sharing less than 10% of total residue content. (wikipedia.org)
Thus, it has been speculated that APH(3') and other protein kinases originate from enzymes from the metabolic pathway for aminoglycosides, and developed in order to counteract the toxic effects of these antibiotics in the host bacterial cell. (wikipedia.org)
Confers resistance1
Selectable marker: plasmid confers resistance to kanamycin (50 μg/ml) in E. coli hosts. (biofeng.com)
Ampicillin2
M15(pREP4) harboring pQE-30 plasmids was grown at 37C or Asunaprevir kinase activity assay 18C in Luria-Bertani (LB) broth or on LB agar supplemented with ampicillin (50 g/ml) and kanamycin (25 g/ml). (insulin-receptor.info)
Contaminated cells had been gathered by centrifugation for 10 min at 3220 × as well as the pellet was resuspended in 30 mL of 2xTY supplemented with 100 μg/mL ampicillin and 50 μg/mL kanamycin (2xTY-AK). (ecologicalsgardens.com)
Inhibitor1
This exceeds the minimum clinically important difference of 5 selleck kinase inhibitor points proposed by Riegel et al (2002). (tyrosinekinases.com)
Promoter1
Going back to the sigE-kanamycin resistant protein, they tried knocking out the sigE promoter, effectively switching all these genes off. (fieldofscience.com)
Plasmid2
strains had been preserved in Luria-Bertani (LB) moderate at 37C with or with no addition of kanamycin (50 g/ml) and chloramphenicol (34 g/ml) when necessary for plasmid selection. (careersfromscience.org)
Removing the sigE operon and placing it on a separate plasmid, that activated resistance to Kanamycin. (fieldofscience.com)
Enzyme1
a novel enzyme that produces (Rosetta (DE3) (Novagen) cells were transformed with the expression vector constructed above and the recombinant cells were cultured in 1?l LB medium containing 10?μg?ml?1 kanamycin at 310?K until the OD600 reached 0.6. (biomasswars.com)
Casein2
The bound complex is internalized and translocates into the oocyte nucleus thanks to an SV40 nuclear localization signal, enhanced by an adjacent casein kinase phosphorylation site. (biologists.com)
Along with S65, S95 is proposed to constitute a casein kinase 1 interaction site while S119 might be modified by cyclin-dependent kinase 5 (Talbot et al. (springer.com)
In the present study, the role of cell-envelop associated eukaryotic serine/threonine protein kinase (STPK) in S. mutans during interspecies competition has been investigated. (elsevierpure.com)
Zhu, L & Kreth, J 2010, ' Role of Streptococcus mutans eukaryotic-type serine/threonine protein kinase in interspecies interactions with Streptococcus sanguinis ', Archives of Oral Biology , vol. 55, no. 5, pp. 385-390. (elsevierpure.com)
Resistant1
Callus proliferated from the galls grew without hormones and in some cases, kanamycin-resistant callus could be cultured. (tamu.edu)
Substrate1
Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell. (nih.gov)
19971
Structures of the autoinhibited SRC family kinases were solved in 1997 revealing, for the first time, the compact arrangement of the SH3 and SH2 domains assembled onto the distal side of the catalytic kinase domain [ 4 ]. (elifesciences.org)
Sensor1
CseC is a sensor (a histadine protein-kinase sensor for those who are interested) while cseB is the response regulator, acting out a response when it receives a signal from cseC. (fieldofscience.com)
Fragment1
In 2015 the BTK SH3-SH2-Kinase fragment was crystallized [ 5 ]. (elifesciences.org)
Agar1
The bacteria were then plated on agar containing antibiotics and challenged with a kanamycin disk. (fieldofscience.com)
Cell2
The resulting clone was grown by adding 500 mL of Luria-Bertani (LB) medium containing 500 μL of 30 mg/mL kanamycin, 25 mL of 10% glucose, and a small amount of transformed cell glycerol stock scraping to a 2 L baffled flask at 30 °C with overnight shaking (250 rpm). (apoptosisblog.com)
Cell wall attacking antibiotics induced kanamycin, whereas antibiotics that attacked (say) the ribosome didn't. (fieldofscience.com)
Dimer2
Membrane-induced dimerization activates BTK and we present here a crystal structure of an activation loop swapped BTK kinase domain dimer that likely represents the conformational state leading to trans-autophosphorylation. (elifesciences.org)
The compact structure of the SH3-SH2-kinase region of BTK is exacted from the domain swapped dimer structure (PDB: 4XI2) solved by Wang et al. (elifesciences.org)
Site2
On the way to activation, disassembly of the SH3-SH2- kinase core opens a new autoinhibitory site on the kinase domain for PHTH domain binding that is ultimately released upon interaction of PHTH with PIP3. (elifesciences.org)
5 ]. The three domains (SH3, SH2, kinase), the SH2-kinase linker, the activation loop and the active site are labeled. (elifesciences.org)
Activity2
The anaerobic cellulolytic bacterium is normally sporadically Asunaprevir kinase activity assay dominant in the rumen (18). (insulin-receptor.info)
XL1-Blue MRF and TOP10F were used as the hosts for pBluescript II SK(+) (Stratagene) and pCRT7-TOPO (Invitrogen) Asunaprevir kinase activity assay cloning, respectively. (insulin-receptor.info)
Date1
The nearest full-length structure of BTK to date consists of the autoinhibited SH3-SH2-kinase core. (elifesciences.org)
Expression1
Recombinant expression, purification, and characterization of Toxoplasma gondii adenosine kinase. (ucdenver.edu)
Data1
Together, these data provide the first structural elucidation of full-length BTK and allow a deeper understanding of allosteric control over the BTK kinase domain during distinct stages of activation. (elifesciences.org)
Total1
This graph shows the total number of publications written about "Adenosine Kinase" by people in this website by year, and whether "Adenosine Kinase" was a major or minor topic of these publications. (ucdenver.edu)
Cells had been cultured in RPMI1640 supplemented with 10% fetal bovine serum, 1% poultry serum, 2 millimeter L-glutamine, 10 Meters 2-mercaptoethanol and 100 g/mL kanamycin in 287383-59-9 5% Company2 at 39 C. DNA transfection and RT-PCR had been performed as previously referred to [22]. (liveconscience.com)