• Many factors and components play a role in the cell cycle of K562 cells in terms of growth, cell differentiation, and apoptosis. (wikipedia.org)
  • The growth of these leukemic cells are controlled by either initiating cell differentiation or apoptosis to occur. (wikipedia.org)
  • These changes can also drive the leukemic cells to a state of stress, which allows for increased sensitivity of the cells to drugs that initiate apoptosis. (wikipedia.org)
  • Apoptosis is an important mechanism in regulating K562 cells and can be induced by the changes in the metabolic state of the cells. (wikipedia.org)
  • There are many different cellular components involved in the cycle of apoptosis such as BCR/ABL, Bcl-2, Bax protein, and cytochrome C. The tumor suppressor gene p53 is also important in the cell cycle regulation of K562 cells. (wikipedia.org)
  • This gene targets the cyclin-dependent kinase inhibitor, p21, and causes cell differentiation, cell cycle arrest in G1, and ultimately apoptosis. (wikipedia.org)
  • When the levels of these components are thrown off, they can either no longer inhibit apoptosis of the cancer cells, a role fulfilled by BCR/ABL, or they cause apoptosis to be induced, in the same vein as Bax and cytochrome C. These components are key in the mitochondria, and due to this, it has been supported that apoptosis uses the mitochondrial apoptosis pathway. (wikipedia.org)
  • Other methods being focused on in the regulation of K562 cells include therapeutic methods like polyphyllin D, which caused differentiation from the progenitor state to occur, and for apoptosis to begin. (wikipedia.org)
  • The effects of S.M. extract on cell viability, production of ROS, nitric oxide (NO), and apoptosis were examined. (unina.it)
  • These potent effects are driven by curcumin's ability to induce G2/M cell cycle arrest, induce autophagy, activate apoptosis, disrupt molecular signaling, inhibit invasion and metastasis, and increase the efficacy of current chemotherapeutics. (hindawi.com)
  • Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
  • In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
  • Gamma-linolenic acid induces apoptosis and lipid peroxidation in human chronic myelogenous leukemia K562 cells. (omega-research.com)
  • Some evidence indicates that polyunsaturated fatty acid can kill cells by apoptosis. (omega-research.com)
  • Flow cytometric analysis also demonstrated that GLA caused dose-dependent apoptosis of K562 cells. (omega-research.com)
  • These results demonstrate that GLA could induce apoptosis in K562 cells. (omega-research.com)
  • AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. (spandidos-publications.com)
  • The result on this occasion was the initiation of apoptosis (programmed cell death) in cancer cells, which shows that curcumin can be an anti-cancer agent. (antiaging-systems.com)
  • Inhibition of DNA-dependent protein kinase catalytic subunit by small molecule inhibitor NU7026 sensitizes human leukemic K562 cells to benzene metabolite-induced apoptosis. (cdc.gov)
  • Sox6-overexpression in K562 cells and in human primary ex vivo erythroid cultures enhances erythroid differentiation and leads to hemoglobinization, the hallmark of erythroid maturation. (edu.sa)
  • To obtain an overview on processes downstream to Sox6 expression, we performed a differential proteomic analysis on human erythroid K562 cells overexpressing Sox6. (edu.sa)
  • Chronic myeloid leukemia (CML) is a myeloproliferative disease that activates multiple signaling pathways, causing cells to produce higher levels of reactive oxygen species (ROS). (unina.it)
  • We can anticipate energetic signaling pathways in K562 one cells using the epigenetic data and concur that the forecasted outcomes highly correlate with real energetic signaling pathways discovered by RNA-seq outcomes. (conferencedequebec.org)
  • KEGG revealed that DEGs were typically enriched in the Rap1 signaling pathway, focal adhesion , proteoglycans and transcriptional misregulation in cancer , signaling pathways regulating pluripotency of stem cells and some immune-related pathways. (bvsalud.org)
  • The dynamic interplay between genes and signaling pathways is associated with TKIs resistance and RHoGDI2 is identified as a biomarker in IR-K562. (bvsalud.org)
  • Following activation, NK cells mediate killing of target cells through two major pathways that require direct contact between NK cells and their target cells ( 6 ). (frontiersin.org)
  • Rab27A is expressed in many human tissues and it regulates the secretion of several types of cellular vesicles, such as melanosomes, insulin granules of pancreatic beta cells, and lytic granules of cytotoxic T lymphocytes. (bdbiosciences.com)
  • Other T lymphocytes (ie, T-suppressor/cytotoxic cells) possess the killing function and also inhibit B-cell development. (medscape.com)
  • K562 cells can spontaneously develop characteristics similar to early-stage erythrocytes, granulocytes and monocytes and are easily killed by natural killer cells as they lack the MHC complex required to inhibit NK activity. (wikipedia.org)
  • Also zinc nanoparticles significantly (p=0.021) inhibit K562 cell line. (ac.ir)
  • Based on this finding it should be concluded that combination of zinc nanoparticles with Saccharomyces cell walls could inhibit the growth of K562 cell line in vitro . (ac.ir)
  • This is supported by pharmacological targeting where the first-generation pan-BET bromodomain inhibitor JQ1(+) displays anti-inflammatory effects and inhibit tumor cell eradication, while the novel bivalent BET bromodomain inhibitor AZD5153, which shows differential activity towards BET family members, does not. (frontiersin.org)
  • In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)
  • In previous research, the authors demonstrated that the methanol extract of A. vulgaris (AVM) has the ability to inhibit the viability of CML cells ( 10 ). (spandidos-publications.com)
  • By colorimetric MTT assay, it was found that contrast media (0-3500 μM) had no effect on both K562 and K562/adr cell viabilities, but in co-treatment with daunorubicin (DNR), diatrizoate decreased cell viability in K562/adr cells by decreasing IC so of DNR from 610.7 ±74.5 nM to 360±108.9 nM. (thescipub.com)
  • The ability to induce these changes in K562 cell cycle and cell cycle regulation provides targets for cancer drugs. (wikipedia.org)
  • DAB2 induction and megakaryocytic differentiation was abrogated when cells were co-treated with the PDGF receptor inhibitor STI571 or when the conditioned medium was derived from TPA-plus STI571-treated cells. (edu.hk)
  • Cell differentiation is induced by the deacetylase activity in these "undifferentiated progenitor cells," which alters the phenotype and morphology of the K562 cells. (wikipedia.org)
  • The change in phenotype induces a decrease in the growth rate and leads the K562 cells to the terminal path of becoming mature erythroids, monocytes, and mature macrophages. (wikipedia.org)
  • 2019). Furthermore to recording concurrent patterns of gene appearance, wide epigenomic profiling in mass cells could anticipate patterns of gene appearance and cell phenotype in response to environmental stimuli (Bock et al. (conferencedequebec.org)
  • Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. (thebiogrid.org)
  • K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. (wikipedia.org)
  • K562 cells were the first human immortalised myelogenous leukemia cell line to be established. (wikipedia.org)
  • K562 cells are part of the NCI-60 cancer cell line panel used by the National Cancer Institute. (wikipedia.org)
  • Another important group of regulators of the K562 line are Sirtuins, referred to as SIRTS. (wikipedia.org)
  • The Leukemia-Lymphoma Cell Line Factsbook. (wikipedia.org)
  • In the present study, we investigated the effect of the marine microalga Skeletonema marinoi (S.M.), a ubiquitous diatom that forms massive blooms in the oceans, on the human leukemia cell line K562. (unina.it)
  • Chronic myeloid leukemia is a common cancer in human, so the goal of this study was the use of natural compound such as cell wall obtained from Saccharomyces cerevisiae (S. cerevisiae) and Saccharomyces boulardi (S. boulardi) and zinc nanoparticles on the growth inhibition of K562 cell line. (ac.ir)
  • The results showed that S. boulardi cell wall significantly (p=0.029) inhibits the growth of K562 cell line compared to S. cerevisiae . (ac.ir)
  • Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. (sigmaaldrich.com)
  • Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. (sigmaaldrich.com)
  • For the cell line mixing experiment, we transfected four replicates each of HCT-116 and K562 cells with 5 µg PB-SRT-Puro and 5 µg HyPBase. (nih.gov)
  • In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
  • SP1 and GATA3) and HDAC2-GATA1 regulatory networks on rescued regions in K562 cell line. (biomedcentral.com)
  • Three studies have shown that monoclonal antibodies to the DENV fusion loop epitope reliably enhanced Zika virus infection (i.e., antibody-dependent enhancement [ADE]) in Fc receptor-bearing K-562 human myelogenous leukemia cells or the U-937 human monocytic cell line ( 1 - 3 ). (cdc.gov)
  • Bioinformatic analysis of the expression profile and identification of RhoGDI2 as a biomarker in imatinib-resistant K562 cells. (bvsalud.org)
  • We performed mRNA and miRNA expression profiling in imatinib -sensitive K562 cells (IS-K562) and imatinib -resistant K562 cells (IR-K562). (bvsalud.org)
  • As a result, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 (human wild‑type) or TCCY‑T315I (human imatinib‑resistant) and the Ba/F3‑(T315I/E279K/Y253H) (mouse BCR/ABL point mutation‑transfected cells). (spandidos-publications.com)
  • Interestingly, AVM appeared to be more sensitive to imatinib‑resistant (T315I, Y253H, and E279K) than wild‑type BCR/ABL cells, indicating its potential to overcome imatinib‑resistant severe issues in CML. (spandidos-publications.com)
  • Thus, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 [human wild-type (WT)] or TCCY-T315I [human imatinib-resistant (IR)] and the Ba/F3-(T315I/E279K/Y253H) (mouse BCR/ABL point mutation-tranfected cells). (spandidos-publications.com)
  • Although Maraffini and colleagues7 recently performed a systematic investigation of Cas9 RGEN specificity in bacteria, the specificities of RGENs in human cells have not been extensively defined and, to our knowledge, bona fide off-target mutations induced by Cas9 have not been identified in any eukaryotic cell or organism. (cdc.gov)
  • 2020). These observations claim that characterization of multiple histone adjustments and DNA-binding protein may have an identical potential also at an individual cell level. (conferencedequebec.org)
  • Nevertheless, technical restrictions in current single-cell epigenomic technology impede a wide profiling of histone adjustments, DNA adjustments, and DNA-binding protein. (conferencedequebec.org)
  • Right here, we examined whether reusable one cells certainly are a useful device for discovering multiple epigenetic marks, including histone adjustments (H3K27ac and H3K27me3), DNA adjustments (5hmC), and genome interacting protein (MED1 and RNA polymerase II) in the same one cell. (conferencedequebec.org)
  • If you require a native form of the protein please use the live cell version - found here . (abcam.com)
  • Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology. (rndsystems.com)
  • Gene expression in benzene-exposed workers by microarray analysis of peripheral mononuclear blood cells: induction and silencing of CYP4F3A and regulation of DNA-dependent protein kinase catalytic subunit in DNA double strand break repair. (cdc.gov)
  • moreover, CYP4F3A mRNA and protein were induced by benzene metabolite phenol in HL-60 and K562 cells as well as ex vivo in human peripheral neutrophils. (cdc.gov)
  • DNA-PKcs mRNA was found consistently increased in the patients and DNA-PKcs mRNA and protein were induced by hydroquinone in HL-60 cells. (cdc.gov)
  • Forkhead box protein M1 (FOXM1) is a key transcription factor (TF) that regulates a common set of genes related to the cell cycle in various cell types. (mdpi.com)
  • Artemis deficiency (with mutations in the Artemis protein resulting in defective VDJ recombination) decreases both T cells and B cells and can be considered part of a subset of SCIDs. (medscape.com)
  • Mutations in the Neuraminidase-Like Protein of Bat Influenza H18N11 Virus Enhance Virus Replication in Mammalian Cells, Mice, and Ferrets. (cdc.gov)
  • In the Fixed Cell ATAC-Seq assay, intact nuclei from formaldehyde-fixed cell samples are treated with a hyperactive Tn5 transposase mutant. (activemotif.com)
  • The Fixed Cell ATAC-Seq Kit from Active Motif provides the reagents necessary to produce 16 unique sequencing-ready Illumina®-compatible ATAC-Seq libraries from 50,000 - 100,000 formaldehyde-fixed cells per reaction. (activemotif.com)
  • The Fixed Cell ATAC-Seq kit contains the necessary reagents and enzymes to perform 16 ATAC-Seq reactions on formaldehyde-fixed cell samples. (activemotif.com)
  • To analyze benzene-caused aberrant gene expression, we examined differential gene expression by microarray analysis of peripheral mononuclear blood cells from seven workers diagnosed with benzene poisoning and seven matched controls. (cdc.gov)
  • Platelet-derived growth factor (PDGF) is involved in megakaryocytopoiesis and is secreted into the culture medium during megakaryocytic differentiation of human leukemic cells. (edu.hk)
  • GO revealed that DEGs were mainly involved in cell adhesion , cell migration , differentiation, and inflammatory response. (bvsalud.org)
  • Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. (southernbiotech.com)
  • Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)
  • Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
  • Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. (sigmaaldrich.com)
  • At higher doses, cells undergo mitochondrial destabilization due to calcium release from the endoplasmic reticulum, and die. (hindawi.com)
  • Herein, we address the complex crosstalk that involves mitochondrial biogenesis, mitochondrial destabilization accompanied by mitophagy, and cell death. (hindawi.com)
  • Potentiation of vincristine-induced growth inhibition by these MFs was also tested in K562/ADM cells by means of WST-1 [2-(4-iodophenyl)-3-(4- nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] assay. (elsevierpure.com)
  • Keep flask in a vertical position until the cells reach the exponential phase of growth. (sigmaaldrich.com)
  • To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. (sigmaaldrich.com)
  • Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. (abcam.com)
  • Moreover, NK cells are poised to release cytokines such as IFN- γ , TNF-α and growth factors that can initiate inflammatory responses mediated by both the innate and the adaptive arm of the immune system. (frontiersin.org)
  • Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
  • Fig. 1: Split deaminases show activity in vitro and in cells. (nature.com)
  • Cell-based and in vitro assays show that Retro-2 blocks delivery of newly-synthesized TA-proteins to the ER-targeting factor ASNA1 (TRC40). (elifesciences.org)
  • Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-g, TNF-a and IL-10, while in vivo ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model. (rndsystems.com)
  • Here we design a high-throughput reporter assay called enhancer × promoter self-transcribing active regulatory region sequencing (ExP STARR-seq) and applied it to examine the combinatorial compatibilities of 1,000 enhancer and 1,000 promoter sequences in human K562 cells. (nature.com)
  • These functions are necessary in cells in order to divide and regenerate tissues, and play a maintenance role in homeostatic functions. (wikipedia.org)
  • Need to perform ATAC-Seq on fresh or frozen cells or tissues? (activemotif.com)
  • GLA treatment could also elevate lipid peroxidation in K562 cells, and antioxidant alpha-tocopherol could reverse the cytotoxicity of GLA. (omega-research.com)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
  • q11)) is the hallmark of chronic myelogenous leukemia (CML) but is also present in 15-30% of adult B-cell acute lymphoblastic. (karger.com)
  • In the current study, we tested the apoptotic effect of GLA on human chronic myelogenous leukemia K562 cells. (omega-research.com)
  • This study observed some iodinated radiographic contrast media, diatrizoate, iopromide and iotrolan affected the cellular energetic state and the kinetics of P-gp in drug-sensitive K562 and drug resistant K562/adr cell lines using spectrophotometer and spectrofluorometer. (thescipub.com)
  • The cells are resistant to 100μM thioguanine and 1mM ouabain. (sigmaaldrich.com)
  • Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
  • Rising single-cell technology can measure the different parts of GRNs today, like the genome, the transcriptome, as well as the proteome (Efremova and Teichmann 2020). (conferencedequebec.org)
  • 2020. Cell Host Microbe. (biolegend.com)
  • 2020. Cell Metabolism. (biolegend.com)
  • We describe here a new reversed-phase high-performance liquid chromatography with mass spectrometry detection method for quantifying intact cytokinin nucleotides in human K-562 leukemia cells. (cas.cz)
  • Chronic myeloid leukemia (CML) is an aggressive malignancy originating from hematopoietic stem cells . (bvsalud.org)
  • Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
  • In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. (southernbiotech.com)
  • Recent work has demonstrated that clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems1-3 can serve as the basis of a simple and highly efficient method for performing genome editing in bacteria, yeast and human cells, as well as in vivo in whole organisms such as fruit flies, zebrafish and mice4-13. (cdc.gov)
  • In contrast, knockdown of BRD4 but not of BRD2 impairs NK cell cytolytic responses, suggesting BRD4 as critical regulator of NK cell mediated tumor cell elimination. (frontiersin.org)
  • Natural killer cells are cytolytic lymphocytes belonging to the innate immune system and are involved in anti-viral and anti-tumor responses ( 1 ) and are recognized as major players in immune-mediated anti-tumor therapies ( 2 ). (frontiersin.org)
  • Furthermore, existing single-cell epigenomic technology cleave genomic DNA and discard the one cells after one use, stopping reanalysis from the same solo cell to verify the full total outcomes and gather data of additional epigenetic grades. (conferencedequebec.org)
  • The Sox6 transcription factor is crucial for terminal maturation of definitive red blood cells. (edu.sa)
  • IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. (prosci-inc.com)
  • Traditionally, B cells have been believed to be the lymphocytes responsible for antibody production via maturation into plasma cells (ie, humoral immunity), and T cells have been believed to be the lymphocytes responsible for killing other cells or organisms (ie, cellular immunity). (medscape.com)
  • Using a chemo-genetic approach, we identify BET bromodomain containing proteins BRD2 and BRD4 as central regulators of NK cell functions, including direct cytokine secretion, NK cell contact-dependent inflammatory cytokine secretion from monocytes as well as NK cell cytolytic functions. (frontiersin.org)
  • However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
  • Typical apoptotic nuclei were shown by staining of K562 cells with DNA-binding fluorochrome Hoechst 33342, characterized by chromatin condensation and nuclear fragmentation. (omega-research.com)
  • Access thousands of knockout cell lysates, generated from commonly used cancer cell lines. (abcam.com)
  • Independent Antibody Validation in Cell Lines (Figure 2) shows similar IL-21 expression profile in human and mouse cell lines detected by two independent anti-IL-21 antibodies that recognize different epitopes, 2465 against central domain and 2463 against C-terminus domain. (prosci-inc.com)
  • IL-21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. (prosci-inc.com)
  • IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. (prosci-inc.com)
  • To this end we used deep sequencing (GRO-seq and PRO-seq) and analyzed nascent mtDNA-encoded RNA transcripts in diverse human cell lines and metazoan organisms. (biorxiv.org)
  • This pausing site correlated with the presence of a bacterial pausing sequence motif, yet the transcription pausing index varied quantitatively among the cell lines. (biorxiv.org)
  • The results showed that 3500 μM diatrizoate decreased ΔΨm from 162.2±0.3 mV to 86.9±9.9 mV in K562/adr cells. (thescipub.com)
  • Results Technique style: a reusable one cell for epigenomic evaluation (REpi-seq) The brand new technique includes two primary sequential techniques. (conferencedequebec.org)
  • Fixed Cell ATAC-Seq in general yields higher background results and lower peak intensity. (activemotif.com)
  • Single cell calling card library preparation was performed using the Nextera Mate Pair Sample Prep Kit with modifications to the manufacturer's protocol. (nih.gov)
  • It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. (biolegend.com)
  • 11. Jacobsen J, Haabeth OW, Tveita AA, Schjetne KW, Munthe LA, Bogen B. Naive idiotope-specific B and T cells collaborate efficiently in the absence of dendritic cells. (southernbiotech.com)
  • Inhibiting these is an important regulation mechanism of cancer, because it prevents cells from progressing into mitosis. (wikipedia.org)
  • THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO 2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO. (sigmaaldrich.com)
  • Natural killer (NK) cells are innate lymphocytes that play a pivotal role in the immune surveillance and elimination of transformed or virally infected cells. (frontiersin.org)
  • Currently, certain T lymphocytes (ie, T-helper cells) are known to be responsible for helping immature B cells develop into mature B cells. (medscape.com)
  • 1] Deoxyguanosine and deoxyadenosine are more toxic in lymphocytes than in other cell types. (medscape.com)
  • GRN510, through a mechanism of telomerase activation, also suppressed the accumulation of senescent cells in the lung. (antiaging-systems.com)
  • 5. Kline GH, Hayden TA, Riegert P. The initiation of B cell clonal expansion occurs independently of pre-B cell receptor formation. (southernbiotech.com)
  • Rees, H. A. & Liu, D. R. Base editing: precision chemistry on the genome and transcriptome of living cells. (nature.com)
  • Genome browser tracks of sorted CD4+ cells from human blood samples compared with K562 cells and ENCODE CD4+ data. (activemotif.com)
  • Eukaryotic cells destroy many endocytosed pathogens by targeting them to the lysosome. (elifesciences.org)
  • Understanding the scope of RGEN-mediated off-target effects in human and other eukaryotic cells will be critically essential if these nucleases are to be used widely for research and therapeutic applications. (cdc.gov)
  • MFs increased the uptake of [ 3 H]vincristine into MDR cells and exhibited MDR-reversing effects. (elsevierpure.com)
  • Cutting edge: Primary and secondary effects of CD19 deficiency on cells of the marginal zone. (southernbiotech.com)
  • To begin to define the specificity determinants of RGENs in human cells, we sought to perform a large-scale test in which we assessed the effects of systematically mismatching various positions within multiple gRNA/target DNA interfaces. (cdc.gov)
  • Frequently, low doses of natural chemical products activate an adaptive stress response, whereas high doses activate acute responses like autophagy and cell death. (hindawi.com)
  • CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation. (biolegend.com)
  • Curcumin causes cell death and primarily initiates an autophagic step (mitophagy). (hindawi.com)