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  • Ligands
  • The L-selectin ligands on HEV are a set of mucin-like glycoproteins, for which glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) is a candidate. (nih.gov)
  • Imparting these modifications to GlyCAM-1, together with appropriate fucosylation, yields enhanced rolling ligands for both peripheral blood lymphocytes and Jurkat cells in flow chamber assays as compared with those generated with exogenous fucosyltransferase. (nih.gov)
  • receptor
  • J.RT3-T3.5 cells have a mutation in the T cell receptor beta chain locus precluding expression of this chain. (wikipedia.org)
  • They do not express surface CD3 or produce the T cell receptor alpha/beta heterodimer. (wikipedia.org)
  • The D1.1 cell line does not express CD4 molecule, an important co-receptor in the activation pathway of helper T cells. (wikipedia.org)
  • The J.gamma1 subline contains no detectable phospholipase C-gamma1 (PLC-γ1) protein and therefore has profound defects in T cell receptor (TCR) calcium mobilization, and nuclear factor of activated T-cells (NFAT) activation (an important transcription factor in T cells). (wikipedia.org)
  • To investigate the requirement for CD2 expression in activation of T lymphocytes via the CD3-Ti antigen/MHC receptor complex, we produced and characterized a series of CD2- Jurkat variants. (rupress.org)
  • Assuming that the Jurkat cell line is representative of normal cycling human T lymphocytes, we conclude that the presence of the CD2 molecule on the plasma membrane is not in itself a requirement for an operational CD3-Ti-alpha/beta receptor. (rupress.org)
  • CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the T cell co-stimulatory receptors OX40, 4-1BB and herpesvirus entry mediator (HVEM). (bpsbioscience.com)
  • It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. (wikipedia.org)
  • It associates with the cytoplasmic tails of the CD4 and CD8 co-receptors on T helper cells and cytotoxic T cells, respectively, to assist signaling from the T cell receptor (TCR) complex. (wikipedia.org)
  • human
  • Recently, three related sulfotransferases (keratan sulfate galactose-6-sulfotransferase [KSGal6ST], high endothelial cell N-acetylglucosamine-6-sulfotransferase [GlcNAc6ST], and human GlcNAc6ST) were cloned, which can generate Gal-6-sulfate and GlcNAc-6-sulfate in GlyCAM-1. (nih.gov)
  • In this study, using the human T-lymphoma cell line, Jurkat cells, we investigated the apoptotic signal transduction mediated by FTY720, in particular comparing its role on the cleavage of caspases, with that mediated by etoposide or anti-Fas antibody. (aspetjournals.org)
  • membrane
  • Though it has not been observed to localize to the cell membrane, it can be found in the outer cell surface. (wikipedia.org)
  • The N-terminal tail of Lck is myristoylated and palmitoylated, which tethers the protein to the plasma membrane of the cell. (wikipedia.org)
  • apoptotic cells
  • Pretreatment with a broad caspase inhibitor [benzyloxycarbonyl-Val-Ala-Asp-(Ome) fluoromethyl ketone] markedly decreased the incidence of apoptotic cells induced by FTY720, etoposide, and anti-Fas antibody, through the abrogation of cleavage of Bid, poly(ADP-ribose) polymerase, and caspases 3, 8, and 9. (aspetjournals.org)
  • Alternatively, apoptotic cells may secrete antigens, including eEF1A and other elongation factors, to induce an autoimmune response during cancer. (wikipedia.org)
  • induces
  • 2-Amino-2-[2-(4-octylphenyl)ethyl] propane-1,3-diol hydrochloride (FTY720), a synthetic product derived from a metabolite of Isaria sinclairii , has been demonstrated to have a potent immunosuppressive activity that induces apoptotic cell death in T cells and several other cell lines. (aspetjournals.org)
  • Thus, these two isoforms demonstrate differences in expression and function: eEF1A1 is expressed in most cells while eEF1A2 is only expressed in adult neuronal and muscle cells, and only eEF1A1 induces HSP70 during heat shock. (wikipedia.org)
  • tumor cells
  • It activates NF-κB and MAPK/JNK signaling upon interaction with its TNF-like ligand, CD70, which is expressed by numerous tumor cells. (bpsbioscience.com)
  • As eEF1A1 is over-expressed in osteoblasts, which proliferate and differentiate in the presence of tumor cells, it may serve as a serum biomarker to track the metastatic progression of prostate cancer. (wikipedia.org)
  • pathways
  • PTPN122 then acts as a switch to convert SHC1 to SgK269-mediated pathways that regulate cell invasion and morphogenesis. (wikipedia.org)
  • fraction
  • Additionally, upon T cell activation, a fraction of kinase active Lck, translocates from outside of lipid rafts (LR) to inside lipid rafts where it interacts with and activates LR-resident Fyn, which is involved in further downstream signaling activation. (wikipedia.org)
  • Adaptor
  • The SHC and its adaptor proteins transmit signaling of the cell surface receptors such as EGFR, erbV-2 and insulin receptors. (wikipedia.org)
  • proteins
  • Lck then phosphorylates and activates ZAP-70, which in turn phosphorylates another molecule in the signaling cascade called LAT (short for Linker of Activated T cells), a transmembrane protein that serves as a docking site for a number of other proteins, the most important of which are Shc-Grb2-SOS, PI3K, and phospholipase C (PLC). (wikipedia.org)
  • SHC proteins are differentially regulated by the Multiple Copies in T-cell malignancy(MCT-1). (wikipedia.org)
  • molecule
  • The T cell surface molecule CD28 can provide costimulatory signals that permit the full activation of T cells. (rupress.org)
  • In addition to its adhesive properties, CD2 also acts as a co-stimulatory molecule on T and NK cells. (wikipedia.org)
  • leukemia
  • Jurkat J6 cells have been found to produce a xenotropic murine leukemia virus (X-MLV) (referred to as XMRV) that could potentially affect experimental outcomes. (wikipedia.org)
  • oxidative
  • When a mutations such as a p66SHC deletion occurs the cardiomyocyte death is reduced and a pool of cardiac stem cells are preserved from oxidative damage - preventing diabetic cardiomyopathy. (wikipedia.org)
  • cardiac
  • mir-143 was found to be the most enriched miRNA in mouse embryonic stem cells that were differentiating into cardiac progenitor cells. (wikipedia.org)
  • It is co-transcribed with miR-145 in cardiac progenitors before becoming vascular smooth muscle cells (VSMCs). (wikipedia.org)
  • line
  • I recently thawed this cell line. (protocol-online.org)
  • This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. (bpsbioscience.com)
  • Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. (bpsbioscience.com)
  • The license does not permit modification of the cell line in any way. (bpsbioscience.com)
  • Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. (bpsbioscience.com)
  • BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. (bpsbioscience.com)
  • Publications using this cell line should reference BPS Bioscience, Inc., San Diego. (bpsbioscience.com)
  • Abstract
  • http://www.cell.com/cancer-cell/abstract/S1535-6108%2814%2900031-2 Ku DH, Chang CD, Koniecki J, Cannizzaro LA, Boghosian-Sell L, Alder H, Baserga R (Apr 1991). (wikipedia.org)
  • protein
  • When expressing the Tet-On 3G transactivator protein from the EF-1 alpha promoter, 83% of the Jurkat Tet-On 3G clones showed strong inducible expression and 33% demonstrated very high inducibility (greater than 2,000-fold). (clontech.com)
  • In TNF-treated cells, TNFR1, TNFR-associated death domain protein (TRADD), Fas-associated death domain protein, and receptor-interacting protein kinase proteins form the signaling complex via modular interaction within their C-terminal death domains. (jimmunol.org)
  • The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M. The FHA domain may be required to interact with phosphorylated proteins. (abcam.com)
  • BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression. (acris-antibodies.com)
  • JNK inhibit of action of B-cell lymphoma protein 2 ( Bcl-2 ), which blocks the release of mitochondrial proteins. (pathwaymaps.com)
  • Caspase 3 is responsible for cellular differentiation, although it is unclear how this kind of protein can promote the cell apoptosis. (wikipedia.org)
  • The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. (wikipedia.org)
  • Within the cell, the process of GroEL/ES mediated protein folding involves multiple rounds of binding, encapsulation, and release of substrate protein. (wikipedia.org)
  • Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. (wikipedia.org)
  • This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. (wikipedia.org)
  • The ability of the retrovirus to bind to its target host cell using specific cell-surface receptors is given by the surface component (SU) of the Env protein, while the ability of the retrovirus to enter the cell via membrane fusion is imparted by the membrane-anchored trans-membrane component (TM). (wikipedia.org)
  • Changes in its DNA sequence transform it into an oncogene that leads to the formation of a different protein with implications for normal cell regulation. (wikipedia.org)
  • Lck (or lymphocyte-specific protein tyrosine kinase) is a 56 kDa protein that is found inside specialized cells of the immune system called lymphocytes. (wikipedia.org)
  • Lck then phosphorylates and activates ZAP-70, which in turn phosphorylates another molecule in the signaling cascade called LAT (short for Linker of Activated T cells), a transmembrane protein that serves as a docking site for a number of other proteins, the most important of which are Shc-Grb2-SOS, PI3K, and phospholipase C (PLC). (wikipedia.org)
  • The N-terminal tail of Lck is myristoylated and palmitoylated, which tethers the protein to the plasma membrane of the cell. (wikipedia.org)
  • inhibit
  • Additionally, NADA has been observed to suppress inflammatory activation of human Jurkat T cells and to inhibit the release of prostaglandin E2 (PGE2) from lipopolysaccharide (LPS)-activated astrocytes, microglia and mouse brain ECs (MEC-Brain). (wikipedia.org)
  • Regulation
  • In humans, homozygous deficiency of any of the early components of the classical pathway of complement activation (C1q, C1r, C1s, C4, and C2) predisposes to the development of SLE ( 9 ), suggesting that complement is involved in removal of dying cells and the immune regulation associated with this process. (jimmunol.org)
  • Fyn is functionally distinct from its family members in that it interacts with FAK and paxillin (PXN) in the regulation of cell morphology and motility. (wikipedia.org)
  • Cancer occurs when a somatic cell which normally cannot divide undergoes mutations which cause de-regulation of the normal cell cycle controls leading to uncontrolled proliferation. (wikipedia.org)
  • pathway
  • The pivotal role of the complement system in the handling of dying cells has been demonstrated for the classical pathway (via C1q) and lectin pathway (via mannose-binding lectin and ficolin). (jimmunol.org)
  • By binding to late apoptotic or necrotic cells, properdin serves as a focal point for local amplification of alternative pathway complement activation. (jimmunol.org)
  • Our data indicate that direct recognition of dying cells by properdin is essential to drive alternative pathway complement activation. (jimmunol.org)
  • Nevertheless, the role of the complement system in the handling of dying cells has been mostly linked to the classical pathway (via C1q) and lectin pathway (via mannose-binding lectin (MBL) and ficolin) ( 10 , 11 , 12 , 13 , 14 ). (jimmunol.org)
  • We found that properdin binds predominantly to late apoptotic and necrotic cells independently of C3b, but not to early apoptotic cells, leading to alternative pathway-mediated complement activation. (jimmunol.org)
  • When this normal biology is compromised, the altered Fyn becomes involved in the neoplastic transformation of normal cells to cancerous ones following the pathway from pre-invasive, to invasive, and ultimately metastasis. (wikipedia.org)
  • kinase
  • IκB kinase β plays a critical role in TNFR1 phosphorylation of S381, which leads to subsequent T cell migration and accumulation. (jimmunol.org)
  • It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. (wikipedia.org)
  • Additionally, upon T cell activation, a fraction of kinase active Lck, translocates from outside of lipid rafts (LR) to inside lipid rafts where it interacts with and activates LR-resident Fyn, which is involved in further downstream signaling activation. (wikipedia.org)
  • Molecular
  • The cellular receptors have not been characterized, but it's hypothesized that it has a molecular structure with near ubiquitous prevalence, since a wide range of cells are permissible to infection. (wikipedia.org)
  • When fyn is activated it causes downstream activation of molecular signals that drive processes crucial to growth and motility of cells. (wikipedia.org)
  • epithelial
  • We found that silencing of INF2 in epithelial RPE-1 cells produced a dramatic drop in tubulin acetylation, increased the G-actin/F-actin ratio, and impaired myocardin-related transcription factor (MRTF)/serum response factor (SRF)-dependent transcription, which is known to be repressed by increased levels of G-actin. (rupress.org)
  • The effect of INF2 silencing on microtubule acetylation was also observed in epithelial ECV304 cells, but not in Jurkat T cells. (rupress.org)
  • apoptotic
  • Herein we report that the only known naturally occurring positive regulator of complement, properdin, binds predominantly to late apoptotic and necrotic cells, but not to early apoptotic cells. (jimmunol.org)
  • Apoptotic cells are a rich source of autoantigens, which are involved in the induction of self-tolerance and autoimmunity ( 4 ). (jimmunol.org)
  • Compelling evidence has emerged that abnormal clearance of apoptotic cells is associated with development of the autoimmune disease systemic lupus erythematosus (SLE) ( 5 , 6 ). (jimmunol.org)
  • Soluble factors from the innate immune system such as complement or pentraxins can opsonize apoptotic cells, thereby promoting their removal by phagocytes ( 6 , 7 , 8 ). (jimmunol.org)
  • Complement-mediated clearance of apoptotic cells has been well documented both in vitro ( 10 ) and in vivo ( 11 ). (jimmunol.org)
  • The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. (wikipedia.org)
  • Per usual in non-apoptotic growing cells caspase activated dnase is held in check inactivated in the cytoplasm thanks to the association with its inhibitor, inhibitor of caspase-activated DNase (ICAD) also known as DNA fragmentation factor 45 kDa (DFF45). (wikipedia.org)
  • Caspase-3 is activated in the apoptotic cell. (wikipedia.org)
  • neurons
  • Eric Dubuis: Cell labelling with Di‐8‐ANEPPS, Fura-2 or Fluo4-AM (for Ca2+ measurements ) or DiI (e.g. retrograde labelling of neurons) Cell Labelling - Eric Dubuis (PDF) . (imperial.ac.uk)
  • BRN3A (POU4F1) is a class IV POU domain-containing transcription factor highly expressed in the developing peripheral sensory nervous system (dorsal root ganglia, trigeminal ganglion, and hindbrain sensory ganglia), certain regions of the central nervous system, retinal neurons called ganglion cells, and in cells of the B- and T-lymphocytic lineages. (wikipedia.org)
  • promoter
  • EF-1 alpha promoter versions of the Tet-On 3G Tetracycline Inducible Expression System provide for consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells. (clontech.com)
  • Concentration
  • In Jurkat T cells, unlike the parent cyclic inosine 5'-diphosphoribose N1-cIDPR 2, 6-thio N1-cIDPR antagonizes both cADPR- and N1-cIDPR-induced Ca(2+) release but possesses weak agonist activity at higher concentration. (ox.ac.uk)
  • unlike
  • In Pseudomonas aeruginosa it causes damages to the cell membrane of these bacteria and, unlike other terpenes, inhibits their proliferation. (wikipedia.org)
  • activation
  • Overall, in uFB cultured HCC cells, a high degree of genetic instability appears to facilitate the inactivation of regular and tumor suppressor proteins at the same time as the activation of HCC relevant proteins like CYP1A and CYP3A. (mek-inhibitors.com)
  • ligand
  • FasR is ubiquitously expressed in various tissues, but its ligand FasL is expressed mainly in activated T lymphocytes and natural killer cells. (pathwaymaps.com)
  • inflammatory
  • Consistently, we observed in inflammatory bowel disease specimens that TNFR1 was constitutively phosphorylated on S381 in those inflammatory T cells, which had accumulated in high numbers in the inflamed mucosa. (jimmunol.org)
  • NADA also promotes the inflammatory resolution of human endothelial cells activated by both endogenous (i.e. (wikipedia.org)
  • vitro
  • He then presented data on HDAC inhibitors in the Gilead's collection, in particular a compound named " GSI-002" which increased histone acetylation in vitro and in vivo without activating T cells, and is well tolerated in rats after a 2-3 weeks dosing: figures 2 & 3. (hiv-reservoir.net)
  • Immortalised cell lines have undergone similar mutations allowing a cell type which would normally not be able to divide to be proliferated in vitro. (wikipedia.org)
  • Immortalized cell lines find use in biotechnology where they are a cost-effective way of growing cells similar to those found in a multicellular organism in vitro. (wikipedia.org)
  • regulates
  • INF2 regulates the circuit, and hence microtubule acetylation, in cell types where it has a prominent role in actin polymerization. (rupress.org)
  • tumor
  • Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation. (acris-antibodies.com)
  • stem cells
  • An immortalized cell line should not be confused with stem cells, which can also divide indefinitely, but form a normal part of the development of a multicellular organism. (wikipedia.org)
  • Some of them are normal cell lines (e.g. derived from stem cells). (wikipedia.org)
  • rats
  • In 2015, another study using rats with carcinogenic DMH injections showed carvacrol at 40 mg/kg bt.w reversed carcinogenic effects on colon cells, however, the therapeutic effects were substantially increased when combined X-radiation treatment. (wikipedia.org)
  • immune
  • Soluble opsonins such as complement can opsonize dying cells, thereby promoting their removal by phagocytes and modulating the immune response. (jimmunol.org)
  • expression
  • This means that in these cell types long term tetracycline inducible expression can be achieved. (clontech.com)
  • We tested the EF-1 alpha version in Jurkat cells, a cell line known to show reduced expression and clonal variation in expression from CMV-based vectors. (clontech.com)
  • cancers
  • Fyn overexpression has been studied in relation to the following cancers: prostate cancer, glioblastoma multiform, squamous cell carcinoma of the head and neck, pancreatic cancer, chronic melogenic leukemia, and melanoma. (wikipedia.org)
  • analysis
  • This allows an analysis to be repeated many times on genetically identical cells which is desirable for repeatable scientific experiments. (wikipedia.org)
  • The alternative, performing an analysis on primary cells from multiple tissue donors, does not have this advantage. (wikipedia.org)
  • Immunity
  • It remains to be determined if this kind of agent is able to enhance immunity to help eliminate HIV-infected cells (figure 5). (hiv-reservoir.net)