The strategies used to block the dimerization were addition of dithiothreitol to the EC1 solution, modification of the Cys thiol with iodoacetamide and polyethylene glycol. (ku.edu)
Iodoacetate3
In comparison with its acid derivative, iodoacetate, iodoacetamide reacts substantially faster. (wikipedia.org)
This observation appears contradictory to standard chemical reactivity, however the presence of a favourable interaction between the positive imidazolium ion of the catalytic histidine and the negatively charged carboxyl-group of the iodoacetate is the reason for the increased relative activity of iodoacetamide. (wikipedia.org)
Iodoacetate or iodoacetamide is very reactive towards free thiols. (creativepegworks.com)
Enzyme2
Crystal structures of the dehalogenase enzyme complexed with iodoacetamide, chloroacetamide, iodide, and chloride at pH 6.2 and 8.2 revealed a halide binding site between the ring NH's of two tryptophan residues, Trp-125 and Trp-175, located in the active site. (rcsb.org)
Enzyme activity was stable at temperatures between 10 and 30°C. The activity of purified catalase was inhibited by azide, cyanide, \beta -mercaptoethanol, dithiotreitol (DTT) and iodoacetamide. (tubitak.gov.tr)
Solution3
Add iodoacetamide solution to a final concentration of 100 mM and incubate for 20 min at room temperature in the dark. (childrenshospital.org)
Add 10 µl prepared Iodoacetamide Solution and 90 µl Urea Solution (no DTT, no SDS). (neb.com)
Prepare Iodoacetamide Solution: add 100 µl prepared Urea Solution (no DTT, no SDS) to 1 tube Iodoacetamide (provided with FASP Kit). (neb.com)
Website1
This graph shows the total number of publications written about "Iodoacetamide" by people in this website by year, and whether "Iodoacetamide" was a major or minor topic of these publications. (umassmed.edu)
Reagents1
Inhibition studies with the reagents iodoacetamide and iodoacetate revealed that iodoacetamide was 15 times as effective as an inhibitor as was iodoacetate. (figshare.com)
Iodoacetamide is an irreversible inhibitor of all cysteine peptidases, with the mechanism of inhibition occurring from alkylation of the catalytic cysteine residue (see schematic). (wikipedia.org)
Cysteine1
Iodoacetamide (IAM) inactivated the DHA reductase activities of RPLTT and C25S, confirming the essential role of cysteine in the reaction mechanism. (nih.gov)
Term1
Marked epithelial hyperplasia of the rat glandular stomach induced by long-term administration of iodoacetamide. (nih.gov)
English1
If you want download english civil war defective rustle, it is movement Iodoacetamide-induced. (pckltdlaw.com)